ChEMBL ligand: CHEMBL334773 (2,3-Bis(4-Hydroxyphenyl)Propanenitrile, Diarylpropionitrile)
Estrogen receptor-α/Estrogen receptor alpha in Human [ChEMBL: CHEMBL206] [GtoPdb: 620] [UniProtKB: P03372] There should be some charts here, you may need to enable JavaScript!
Estrogen receptor-β/Estrogen receptor beta in Human [ChEMBL: CHEMBL242] [GtoPdb: 621] [UniProtKB: Q92731] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Estrogen receptor-α/Estrogen receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL206] [GtoPdb: 620] [UniProtKB: P03372]
ChEMBL	Antagonist activity at estrogen receptor alpha (unknown origin) assessed as inhibition of estradiol-induced response after 22 hrs by cell-based luciferase reporter gene assay	B	5	pEC50	>10000	nM	EC50	J Med Chem (2018) 61: 4720-4738 [PMID:29741891]
ChEMBL	Agonist activity at estrogen receptor alpha (unknown origin) after 22 hrs by cell-based luciferase reporter gene assay	B	5.64	pEC50	2300	nM	EC50	J Med Chem (2018) 61: 4720-4738 [PMID:29741891]
ChEMBL	Agonist activity at human N-His6-tagged terbium-labelled ERalpha ligand binding domain expressed in Escherichia coli BL21 cells assessed as recruitment of fluorescein-labelled SRC3 after 1 hr by TR-FRET assay	B	6.06	pEC50	876	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
ChEMBL	Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	F	6.52	pEC50	304	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
ChEMBL	Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	F	6.68	pEC50	208	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
GtoPdb	-	-	7.18	pEC50	66	nM	EC50	Brain Res (2009) 1290: 1-11 [PMID:19596275]
ChEMBL	Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	F	7.18	pEC50	66	nM	EC50	Eur J Med Chem (2009) 44: 3412-3424 [PMID:19286283]
ChEMBL	Transcriptional potency (EC50) at Human estrogen receptor alpha	B	7.7	pEC50	20	nM	EC50	J Med Chem (2001) 44: 4230-4251 [PMID:11708925]
ChEMBL	Agonist activity at human N-His6-tagged terbium-labelled NRID-SRC3 of ERalpha ligand binding domain expressed in Escherichia coli BL21 cells after 1 hr by TR-FRET assay	B	8.47	pEC50	3.4	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
Estrogen receptor-β/Estrogen receptor beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL242] [GtoPdb: 621] [UniProtKB: Q92731]
GtoPdb	-	-	8.6	pKi	-	-	-	J Med Chem (2000) 43: 4934-47 [PMID:11150164]; J Med Chem (2001) 44: 4230-51 [PMID:11708925]
ChEMBL	Antagonist activity at estrogen receptor beta (unknown origin) assessed as inhibition of estradiol-induced response after 22 hrs by cell-based luciferase reporter gene assay	B	5	pEC50	>10000	nM	EC50	J Med Chem (2018) 61: 4720-4738 [PMID:29741891]
ChEMBL	Estrogenic activity at ERbeta (unknown origin) expressed in human MDA-MB-231/beta41 cells after 18 hrs by renilla luciferase reporter gene assay	B	7.7	pEC50	20	nM	EC50	J Nat Prod (2018) 81: 966-975 [PMID:29641206]
ChEMBL	Agonist activity at human N-His6-tagged terbium-labelled ERbeta ligand binding domain expressed in Escherichia coli BL21 cells assessed as recruitment of fluorescein-labelled SRC3 after 1 hr by TR-FRET assay	B	7.78	pEC50	16.7	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
ChEMBL	Transcriptional potency (EC50) at Human estrogen receptor Beta	B	8.22	pEC50	6	nM	EC50	J Med Chem (2001) 44: 4230-4251 [PMID:11708925]
ChEMBL	Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	F	8.43	pEC50	3.7	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
ChEMBL	Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	F	8.48	pEC50	3.3	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]
ChEMBL	Binding affinity to human GST-tagged estrogen receptor beta ligand binding domain after 1 hr by TR-FRET assay	B	8.72	pEC50	1.9	nM	EC50	J Med Chem (2018) 61: 4720-4738 [PMID:29741891]
ChEMBL	Agonist activity at estrogen receptor beta (unknown origin) after 22 hrs by cell-based luciferase reporter gene assay	B	8.96	pEC50	1.1	nM	EC50	J Med Chem (2018) 61: 4720-4738 [PMID:29741891]
GtoPdb	-	-	9.07	pEC50	0.85	nM	EC50	Brain Res (2009) 1290: 1-11 [PMID:19596275]
ChEMBL	Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	F	9.07	pEC50	0.85	nM	EC50	Eur J Med Chem (2009) 44: 3412-3424 [PMID:19286283]
ChEMBL	Agonist activity at human ERbeta receptor expressed in HEK293T cells transfected with pGL4.27-(ERE)3-Luc assessed as transcriptional activation measured after 16 hrs by Dual-Glo luciferase assay	B	9.09	pEC50	0.82	nM	EC50	Bioorg Med Chem (2016) 24: 5895-5910 [PMID:27692995]
ChEMBL	Agonist activity at human N-His6-tagged terbium-labelled NRID-SRC3 of ERbeta ligand binding domain expressed in Escherichia coli BL21 cells after 1 hr by TR-FRET assay	B	9.15	pEC50	0.71	nM	EC50	J Med Chem (2012) 55: 528-537 [PMID:22122563]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]