ChEMBL ligand: CHEMBL273094
A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] Adenosine A1 receptor in Bovine [ChEMBL: CHEMBL4975] [UniProtKB: P28190] A1 receptor/Adenosine A1 receptor in Rat [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099] Adenosine A1 receptor in Guinea pig [ChEMBL: CHEMBL2304404] [UniProtKB: P47745] There should be some charts here, you may need to enable JavaScript!
A2A receptor/Adenosine A2a receptor in Human [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274] A2A receptor/Adenosine A2a receptor in Rat [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543] There should be some charts here, you may need to enable JavaScript!
A2B receptor/Adenosine A2b receptor in Human [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275] There should be some charts here, you may need to enable JavaScript!
A3 receptor/Adenosine A3 receptor in Human [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8] A3 receptor/Adenosine A3 receptor in Rat [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
ChEMBL	The A1-StaR receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore.	B	7.83	pKd	14.68	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	Displacement of [3H]DPCPX from human adenosine A1 receptor expressed in human CHO cells incubated for 30 mins by fluorescence polarization binding assay	B	4.54	pKi	29100	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Displacement of [3H]DPCPX from human A1AR expressed in CHO cell membrane incubated for 60 mins by microbeta trilux scintillation counter analysis	B	7.54	pKi	29.1	nM	Ki	J Med Chem (2022) 65: 2091-2106 [PMID:35068155]
ChEMBL	Displacement of MRS7416 from recombinant human adenosine receptor A1 expressed in HEK293 cell membranes after 1 hr by FACS analysis	B	7.76	pKi	17.2	nM	Ki	Medchemcomm (2017) 8: 1659-1667 [PMID:29250307]
ChEMBL	Displacement of [3H]DPCPX from human recombinant adenosine A1 receptor expressed in HEK293T cells	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2007) 15: 3235-3240 [PMID:17350268]
ChEMBL	Displacement of [3H]DPCPX from human recombinant adenosine A1 receptor expressed in HEK293T cells	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2008) 16: 3825-3830 [PMID:18262425]
ChEMBL	Displacement of [3H]DPCPX from human recombinant adenosine A1 receptor expressed in HEK293T cells by scintillation counting	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2009) 17: 4280-4284 [PMID:19481943]
ChEMBL	Antagonist activity at human adenosine A1 receptor assessed as cAMP level by cell based assay	F	8.08	pKi	8.3	nM	Ki	Bioorg Med Chem Lett (2013) 23: 3427-3433 [PMID:23602401]
ChEMBL	Antagonist activity against recombinant human adenosine A1 receptor expressed in HEK293 cells in presence of [125I]IABA radioligand.	F	8.17	pKi	6.82	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
Adenosine A1 receptor in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4975] [UniProtKB: P28190]
ChEMBL	Binding affinity at bovine Adenosine A1 receptor.	B	8.89	pKi	1.3	nM	Ki	J Med Chem (2002) 45: 4875-4887 [PMID:12383013]
ChEMBL	Ability to displace the specific binding of [3H]CHA to adenosine A1 receptor from bovine brain cortical membranes	B	8.89	pKi	1.3	nM	Ki	J Med Chem (2000) 43: 2814-2823 [PMID:10956189]
ChEMBL	Affinity for Adenosine A1 receptor using [N6-[(3-[125I]-iodo-4-aminophenyl)ethyl]adenosine in calf brain	B	9.52	pKi	0.3	nM	Ki	J Med Chem (1989) 32: 1043-1051 [PMID:2709373]
A1 receptor/Adenosine A1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099]
ChEMBL	Binding affinity towards rat Adenosine A1 receptor	B	8.89	pKi	1.3	nM	Ki	J Med Chem (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Displacement of [3H]DPCPX binding to adenosine A1 receptor of rat brain cortical membrane	B	8.92	pKi	1.2	nM	Ki	J Med Chem (2001) 44: 749-762 [PMID:11262085]
ChEMBL	Binding affinity towards adenosine A1 receptor in rat cortical membranes using N6-[3H]cyclohexyladenosine as radioligand	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1992) 35: 3066-3075 [PMID:1501234]
ChEMBL	Inhibition of [3H]- PIA binding at adenosine A1 receptor on rat cerebral cortex membranes.	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1987) 30: 211-214 [PMID:3806597]
ChEMBL	Displacement of [3H]PIA from adenosine A1 receptors of rat brain membrane	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1994) 37: 3373-3382 [PMID:7932565]
ChEMBL	Binding affinity for adenosine A1 receptor using [3H]- CHA or [3H]- PIA	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1992) 35: 407-422 [PMID:1738138]
ChEMBL	Binding affinity towards adenosine A1 receptor in rat cerebral cortical membranes by [3H]cyclohexyladenosine displacement.	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1985) 28: 1334-1340 [PMID:2993622]
ChEMBL	Antagonist activity against adenosine A1 receptor in rat brain membrane in presence of [3H]R-PIA radioligand.	F	8.92	pKi	1.2	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Inhibition of binding of 1 nM [3H]N-6-(phenylisopropyl)adenosine to adenosine A1 receptor in rat cortical membranes	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1989) 32: 1873-1879 [PMID:2754711]
ChEMBL	Inhibition of (R)-N6-([3H]-phenylisopropyl) adenosine binding to adenosine A1 receptor from rat cortical membranes	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1992) 35: 2342-2345 [PMID:1613758]
ChEMBL	Affinity for adenosine A1 receptor using [3H]N6-(phenylisopropyl)adenosine (R)-[3H]-PIA as a radioligand in rat brain.	B	8.96	pKi	1.1	nM	Ki	J Med Chem (1989) 32: 1043-1051 [PMID:2709373]
Adenosine A1 receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2304404] [UniProtKB: P47745]
ChEMBL	Binding affinity against adenosine A1 receptor in guinea pig forebrain membranes using N6-[3H]cyclohexyladenosine as radioligand	B	7.96	pKi	11	nM	Ki	J Med Chem (1992) 35: 3066-3075 [PMID:1501234]
ChEMBL	Binding affinity against adenosine A1 receptor from guinea pig forebrain membranes, using N6-[3H]cyclohexyladenosine as radioligand.	B	7.96	pKi	11	nM	Ki	J Med Chem (1992) 35: 2342-2345 [PMID:1613758]
A2A receptor/Adenosine A2a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274]
ChEMBL	The A2A-StaR2 receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore. A minimum of three measurements were recorded for each interaction.	B	7.12	pKd	76.29	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	Displacement of [3H]ZM241385 from stabilized human adenosine receptor A2a expressed in HEK293 cells followed by receptor capturing on Biocore chips by SPR method	B	7.7	pKd	19.95	nM	Kd	J Med Chem (2011) 54: 4312-4323 [PMID:21661720]
ChEMBL	The A2A-StaR2 receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Then it has been reconstituted into rHDLs membrane discs.Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore. A minimum of three measurements were recorded for each interaction.	B	7.73	pKd	18.46	nM	Kd	K4DD drug target binding kinetics data
GtoPdb	-	-	8	pKd	-	-	-	J Biol Chem (1995) 270: 13987-97 [PMID:7775460]
ChEMBL	Displacement of [3H] 4-2-7-amino-2-2-furyl1,2,4 triazolo2,3-a1,3,5triazin-5-ylaminoethyl phenol from human adenosine A2A receptor expressed in human HeLa cells incubated for 30 mins by fluorescence polarization binding assay	B	6	pKi	1000	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Ability to displace the specific binding of [3H]-CGS- to adenosine A2A receptor form bovine brain striatal membranes	B	7.2	pKi	63	nM	Ki	J Med Chem (2000) 43: 2814-2823 [PMID:10956189]
ChEMBL	Displacement of MRS5346 from C-terminal 10xHis and 1D4-tagged C-terminal-truncated human A2A adenosine receptor (1 to 316 residues) expressed in Pichia pastoris expression system after 1 hr by fluorescence polarization assay	B	7.3	pKi	50.1	nM	Ki	ACS Med Chem Lett (2017) 8: 660-665 [PMID:28626529]
ChEMBL	Binding affinity for HA-tagged mutant human Adenosine A2A receptor (V84L), using [3H]CGS-21680 as radioligand expressed in COS-7 cells	B	7.34	pKi	45.6	nM	Ki	J Med Chem (1997) 40: 2588-2595 [PMID:9258366]
ChEMBL	Displacement of [3H]ZM241385 from stabilized human adenosine receptor A2a expressed in HEK293 cells followed by receptor capturing on Biocore chips by SPR method	B	7.6	pKi	25.12	nM	Ki	J Med Chem (2011) 54: 4312-4323 [PMID:21661720]
ChEMBL	Antagonist activity against recombinant human adenosine A2A receptor expressed in HEK293 cells in presence of [125I]iodo-ZM-241385 radioligand.	F	7.74	pKi	18.4	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Binding affinity for HA-tagged mutant human Adenosine A2A receptor (H250N) using [3H]-CGS-21,680 as radioligand expressed in COS-7 cells	B	7.81	pKi	15.6	nM	Ki	J Med Chem (1997) 40: 2588-2595 [PMID:9258366]
ChEMBL	Binding affinity for HA-tagged wild type human Adenosine A2A receptor (WT) using [3H]CGS-21680 as radioligand expressed in COS-7 cells	B	8.1	pKi	7.89	nM	Ki	J Med Chem (1997) 40: 2588-2595 [PMID:9258366]
ChEMBL	Antagonist activity at human adenosine A2A receptor assessed as cAMP level by cell based assay	F	8.24	pKi	5.8	nM	Ki	Bioorg Med Chem Lett (2013) 23: 3427-3433 [PMID:23602401]
ChEMBL	Competitive binding affinity to human adenosine A2A receptor expressed in HEK293 cells after 60 mins by fluorescence polarization assay in presence of MRS5346	B	8.43	pKi	3.7	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [3H]CGS21680 from human adenosine A2A receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting analysis	B	9	pKi	1	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [3H]HZ241385 from human A2AAR expressed in HeLa cell membrane incubated for 30 mins by microbeta trilux scintillation counter analysis	B	9	pKi	1	nM	Ki	J Med Chem (2022) 65: 2091-2106 [PMID:35068155]
A2A receptor/Adenosine A2a receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543]
ChEMBL	Antagonist activity against adenosine A2A receptor in rat striatal membrane in presence of [3H]CGS-21680 radioligand.	F	7.2	pKi	63	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Displacement of [3H]ZM-241385 binding in adenosine A2A receptor of rat striatal membrane	B	7.2	pKi	63	nM	Ki	J Med Chem (2001) 44: 749-762 [PMID:11262085]
ChEMBL	Displacement of [3H]-CGS- 21680 from adenosine A2a receptors of rat striatal membrane	B	7.2	pKi	63	nM	Ki	J Med Chem (1994) 37: 3373-3382 [PMID:7932565]
ChEMBL	Binding affinity towards rat Adenosine A2A receptor	B	7.2	pKi	63	nM	Ki	J Med Chem (2002) 45: 1500-1510 [PMID:11906291]
A2B receptor/Adenosine A2b receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A2B receptor expressed in human HEK293 cells incubated for 30 mins by fluorescence polarization binding assay	B	3.85	pKi	141000	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Displacement of [3H]DPCPX from human A2BAR expressed in HEK293 cell membrane incubated for 30 mins by microbeta trilux scintillation counter analysis	B	6.85	pKi	141	nM	Ki	J Med Chem (2022) 65: 2091-2106 [PMID:35068155]
ChEMBL	Binding affinity towards human Adenosine A2B receptor	B	7.32	pKi	48	nM	Ki	J Med Chem (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Antagonist activity against human A2B adenosine receptor expressed in HEK293 cells uisng [3H]ZM-241385 or [125I]-IABOPX	F	8.11	pKi	7.75	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
A3 receptor/Adenosine A3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8]
ChEMBL	Displacement of [3H]NECA from human adenosine A3 receptor expressed in human HeLa cells incubated for 30 mins by fluorescence polarization binding assay	B	4.04	pKi	91900	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Displacement of CELT-228 from human adenosine A3 receptor expressed in human HeLa cells incubated for 30 mins by fluorescence polarization binding assay	B	4.6	pKi	25300	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Binding affinity at Mutant (H272E) human adenosine A3 receptor expressed in COS-7 cells	B	4.7	pKi	20100	nM	Ki	J Med Chem (2001) 44: 4125-4136 [PMID:11708915]
ChEMBL	Binding affinity at wild-type Adenosine A3 receptor expressed in COS-7 cells	B	6.96	pKi	110	nM	Ki	J Med Chem (2001) 44: 4125-4136 [PMID:11708915]
ChEMBL	Displacement of [125I]AB-MECA from human adenosine A3 receptor expressed in HEK 293 cells	B	6.96	pKi	110	nM	Ki	J Med Chem (2001) 44: 749-762 [PMID:11262085]
ChEMBL	Displacement of [3H]NECA from human A3AR expressed in HeLa cell membrane incubated for 180 mins by microbeta trilux scintillation counter analysis	B	7.04	pKi	91.9	nM	Ki	J Med Chem (2022) 65: 2091-2106 [PMID:35068155]
ChEMBL	Binding affinity for human Adenosine A3 receptor	B	7.15	pKi	70	nM	Ki	J Med Chem (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Antagonist activity against human adenosine A3 receptor expressed in HEK cells in presence of [125]IAB-MECA or [125I]IABA radioligand.	F	7.59	pKi	25.6	nM	Ki	J Med Chem (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Competitive binding affinity to human adenosine A3 receptor expressed in CHO cells after 90 mins by flow cytometric analysis in presence of MRS5449	B	7.7	pKi	19.9	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [125I]I-AB-MECA from human adenosine A3 receptor expressed in CHO cells after 90 mins by gamma counting analysis	B	7.86	pKi	13.8	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
A3 receptor/Adenosine A3 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647]
ChEMBL	Displacement of [125I]AB-MECA from membranes of CHO cells stably transfected with the rat adenosine A3 receptor cDNA	B	4.54	pKi	29000	nM	Ki	J Med Chem (1994) 37: 3373-3382 [PMID:7932565]
ChEMBL	Ability to displace the specific binding of [3H](R)-PIA to adenosine A3 receptor from rat testis membranes	B	6.38	pKi	418	nM	Ki	J Med Chem (2000) 43: 2814-2823 [PMID:10956189]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay	F	4.9	pIC50	12589.25	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay	F	4.9	pIC50	12589.25	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum Dd2 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]