ChEMBL ligand: CHEMBL184238 (KDS-4103, ORG-231295, Urb-597, URB 597, URB597)
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519] Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914] Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612] There should be some charts here, you may need to enable JavaScript!
Cyclooxygenase-1 in Sheep [ChEMBL: CHEMBL2949] [UniProtKB: P05979] There should be some charts here, you may need to enable JavaScript!
COX-2 /Cyclooxygenase-2 in Human [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354] There should be some charts here, you may need to enable JavaScript!
epoxide hydrolase 2/Epoxide hydratase in Human [ChEMBL: CHEMBL2409] [GtoPdb: 2970] [UniProtKB: P34913] There should be some charts here, you may need to enable JavaScript!
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7] There should be some charts here, you may need to enable JavaScript!
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519]
ChEMBL	Inhibition of human recombinant FAAH expressed in HEK293 cells by [3H]anandamide carbon filtration assay	B	5.66	pIC50	2187	nM	IC50	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of recombinant human N-terminal -His6 tagged FAAH (32 to 579 residues) expressed in Escherichia coli BL21-AI preincubated for 5 mins followed by olamide substrate addition measured every 10 sec intervals for 30 mins by spectrophotometry	B	6.01	pIC50	986	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
ChEMBL	Inhibition of human recombinant FAAH expressed in sf21 cells using AMC-AA as substrate preincubated for 15 mins followed by substrate addition measured after 2 hrs by spectrophotometric analysis	B	6.59	pIC50	260	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2701-2705 [PMID:27117424]
ChEMBL	Inhibition of human recombinant FAAH overexpressed in African green monkey COS7 cells using [3H]-anandamide as substrate preincubated for 10 mins before substrate addition measured after 10 mins by liquid scintillation counting analysis	B	6.77	pIC50	170	nM	IC50	J Med Chem (2013) 56: 8484-8496 [PMID:24083878]
ChEMBL	Inhibition of human recombinant FAAH overexpressed in African green monkey COS7 cells using [3H]-anandamide as substrate preincubated for 10 mins before substrate addition measured after 10 mins by liquid scintillation counting analysis	B	6.78	pIC50	165.96	nM	IC50	J Med Chem (2013) 56: 8484-8496 [PMID:24083878]
ChEMBL	Inhibition of recombinant human N-terminal -His6 tagged FAAH (32 to 579 residues) expressed in Escherichia coli BL21 preincubated for 60 mins followed by olamide substrate addition measured every 10 sec intervals for 30 mins by spectrophotometry	B	6.96	pIC50	109	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
ChEMBL	Inhibition of FAAH	B	6.96	pIC50	109	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
GtoPdb	-	-	7	pIC50	100	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]
ChEMBL	Inhibition of human recombinant FAAH in assessed as hydrolysis of [14C]-anandamide preincubated for 20 mins before [14C]-anandamide addition measured after 30 mins	B	7.05	pIC50	90	nM	IC50	ACS Med Chem Lett (2012) 3: 182-186 [PMID:24900454]
ChEMBL	Inhibition of human FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	7.11	pIC50	78	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of human recombinant FAAH using AMC arachidonoyl amide as substrate after 30 mins by fluorescence assay	B	7.23	pIC50	59	nM	IC50	Eur J Med Chem (2015) 97: 289-305 [PMID:26002335]
ChEMBL	Inhibition of human recombinant FAAH using AMC arachidonoyl amide as substrate preincubated with protein for 0 min followed by substrate addition by fluorescence assay	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2015) 97: 289-305 [PMID:26002335]
ChEMBL	Inhibition of human recombinant FAAH expressed in HEK293 cells preincubated for 5 mins before substrate addition by [3H]anandamide carbon filtration assay	B	7.33	pIC50	47	nM	IC50	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of human recombinant FAAH expressed in Escherichia coli using [3H]AEA substrate incubated at 37 degC for 10 mins by liquid scintillation counting method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2011) 19: 3777-3786 [PMID:21612933]
ChEMBL	Inhibition of human FAAH using N-(6-methoxypyridin-3-yl)octanamide as a substrate assessed as reduction in 6-methoxypyridin-3-amine formation by fluorescence microplate reader assay	B	7.49	pIC50	32	nM	IC50	Bioorg Med Chem (2021) 51: 116507-116507 [PMID:34794001]
ChEMBL	Inhibition of human FAAH expressed in baculovirus-infected High Five cells S9 fraction using OMP substrate by fluorescence based assay	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2018) 28: 762-768 [PMID:29366648]
ChEMBL	Inhibition of human recombinant FAAH expressed in HEK293 cells preincubated for 30 mins before substrate addition by [3H]anandamide carbon filtration assay	B	7.8	pIC50	16	nM	IC50	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of human recombinant FAAH expressed in Escherichia coli assessed as hydrolysis of [3H]-AEA	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1322-1326 [PMID:24508127]
ChEMBL	Inhibition of human recombinant FAAH using AMC arachidonoyl amide as substrate preincubated with protein for 30 mins followed by substrate addition by fluorescence assay	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2015) 97: 289-305 [PMID:26002335]
ChEMBL	Inhibition of human recombinant FAAH expressed in HEK293 cells preincubated for 60 mins before substrate addition by [3H]anandamide carbon filtration assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of human recombinant FAAH using AMC arachidonoyl amide as substrate preincubated with protein for 60 mins followed by substrate addition by fluorescence assay	B	8.16	pIC50	6.9	nM	IC50	Eur J Med Chem (2015) 97: 289-305 [PMID:26002335]
ChEMBL	Inhibition of human FAAH	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2008) 51: 7327-7343 [PMID:18983142]
ChEMBL	Inhibition of FAAH	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2012) 55: 6898-6915 [PMID:22779702]
ChEMBL	Inhibition of FAAH	B	8.34	pIC50	4.6	nM	IC50	Bioorg Med Chem Lett (2011) 21: 4674-4685 [PMID:21764305]
ChEMBL	Irreversible inhibition of human recombinant FAAH assessed as hydrolysis of [3H]-AEA after 10 mins by liquid scintillation counting	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2011) 54: 6812-6823 [PMID:21899370]
ChEMBL	Inhibition of human FAAH assessed as hydrolysis of anandamido-amino-methyl-cumarin	B	8.4	pIC50	4	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2492-2496 [PMID:21392988]
ChEMBL	Inhibition of FAAH (unknown origin)	B	8.4	pIC50	4	nM	IC50	Bioorg Med Chem (2013) 21: 28-41 [PMID:23218778]
ChEMBL	Inhibition of FAAH (unknown origin)	B	8.42	pIC50	3.8	nM	IC50	Eur J Med Chem (2013) 63: 64-75 [PMID:23455058]
ChEMBL	Inhibition of human FAAH expressed in HEK293-TRex cells using arachadonyl 7-amino 4-methyl coumarin amide as substrate by fluorescence assay	B	8.52	pIC50	3	nM	IC50	Medchemcomm (2012) 3: 1258-1263
ChEMBL	Inhibition of human FAAH expressed in HEK293 cells using arachadonyl 7-amino 4-methyl coumarin amide as substrate by fluorimetric assay	B	8.64	pIC50	2.3	nM	IC50	Medchemcomm (2012) 3: 611-619
ChEMBL	Inhibition of MBP-fused human recombinant FAAH with truncated N-terminal transmembrane domain expressed in Escherichia coli T7 using D-AMC substrate measured over 40 mins by fluorescence based assay	B	9.62	pIC50	0.24	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2965-2973 [PMID:27130358]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914]
ChEMBL	Inhibition of mouse FAAH isolated from brain homogenate using [3H-ethanolamine]AEA as substrate incubated for 20 mins prior to substrate addition measured after 15 mins by beta counting analysis	B	8.92	pKi	1.2	nM	Ki	J Med Chem (2012) 55: 6898-6915 [PMID:22779702]
ChEMBL	Inhibition of FAAH in mouse brain membranes using [14C]-AEA as substrate incubated for 15 mins by scintillation counting method	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2019) 183: 111674-111674 [PMID:31518969]
ChEMBL	Inhibition of FAAH in mouse brain microsomes using OMP substrate by fluorescence based assay	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2018) 28: 762-768 [PMID:29366648]
ChEMBL	Inhibition of mouse FAAH isolated from brain homogenate using [3H-ethanolamine]AEA as substrate incubated for 20 mins prior to substrate addition measured after 15 mins by beta counting analysis	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2012) 55: 6898-6915 [PMID:22779702]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612]
ChEMBL	Inhibition of rat cortex FAAH by [3H]anandamide carbon filtration assay	B	8.4	pKi	4	nM	Ki	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of FAAH from rat brain microsomes by RP-HPLC	B	6.62	pIC50	240	nM	IC50	Bioorg Med Chem (2010) 18: 945-952 [PMID:20005725]
ChEMBL	Inhibition of FAAH in rat brain membrane at pH 7.4 using N-arachidonoyl-[14C]-ethanolamine as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by scintillation counting method	B	6.85	pIC50	140.8	nM	IC50	J Med Chem (2016) 59: 6387-6406 [PMID:27309570]
ChEMBL	Inhibition of Sprague-Dawley rat brain microsome FAAH assessed as reduction in 4-pyren-1-ylbutanoic acid release after 60 mins by reverse phase HPLC-based fluorescence method	B	7.17	pIC50	67	nM	IC50	Bioorg Med Chem (2012) 20: 1100-1112 [PMID:22196515]
ChEMBL	Inhibition of rat brain FAAH using [3H]AEA as substrate preincubated for 10 mins at pH 6	B	7.19	pIC50	64	nM	IC50	Eur J Med Chem (2017) 136: 523-542 [PMID:28535469]
ChEMBL	Inhibition of rat FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	7.2	pIC50	63	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of FAAH in Sprague-Dawley rat brain microsomes assessed as N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide conversion to 4-pyren-1-ylbutanoic acid preincubated for 10 mins measured after 45 mins by HPLC analysis	B	7.21	pIC50	62	nM	IC50	Eur J Med Chem (2013) 63: 64-75 [PMID:23455058]
ChEMBL	Inhibition of FAAH in rat brain microsomes assessed as reduction in enzyme-mediated 4-pyren-1-ylbutanoic acid release using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate incubated fro 60 mins by reversed phase HPLC method	B	7.22	pIC50	60	nM	IC50	Medchemcomm (2017) 8: 616-620 [PMID:30108777]
ChEMBL	Inhibition of FAAH in rat brain microsomes using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate after 60 mins by fluorescence-based reversed phase HPLC analysis	B	7.22	pIC50	60	nM	IC50	Bioorg Med Chem (2017) 25: 825-837 [PMID:27989417]
ChEMBL	Inhibition of FAAH in rat brain microsomes using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate after 60 mins by fluorescence-based HPLC analysis	B	7.22	pIC50	60	nM	IC50	Eur J Med Chem (2018) 160: 183-192 [PMID:30340141]
ChEMBL	Inhibition of Sprague-Dawley rat brain microsomal FAAH using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate assessed as 4-pyren-1-ylbutanoic acid release preincubated for 10 mins with substrate followed by protein addition measured after 60 mins by reverse phase HPLC with fluorescence detection method	B	7.22	pIC50	60	nM	IC50	Medchemcomm (2014) 5: 932-936
ChEMBL	Inhibition of rat FAAH preincubated for 20 mins	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4838-4843 [PMID:18693015]
ChEMBL	Inhibition of FAAH in rat brain membrane using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate measured after 60 mins by reverse-phase HPLC-based fluorescence assay	B	7.37	pIC50	43	nM	IC50	J Med Chem (2020) 63: 13159-13186 [PMID:33089691]
ChEMBL	Inhibition of FAAH in rat brain membrane at pH 9 using N-arachidonoyl-[14C]-ethanolamine as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by scintillation counting method	B	7.63	pIC50	23.5	nM	IC50	J Med Chem (2016) 59: 6387-6406 [PMID:27309570]
ChEMBL	Inhibition of rat FAAH incubated 3 hrs prior to addition of arachidonyl-amino-methyl-coumarin amide	B	7.66	pIC50	22	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of rat brain FAAH using [3H]AEA as substrate preincubated for 10 mins at pH 8	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2017) 136: 523-542 [PMID:28535469]
ChEMBL	Inhibition of FAAH in rat brain microsomes using OMP substrate by fluorescence based assay	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem Lett (2018) 28: 762-768 [PMID:29366648]
ChEMBL	High Throughput FAAH Inhibition Assays: The assays for compounds described herein are amenable to high throughput screening. Preferred assays thus detect binding of the inhibitor to FAAH or the release of a reaction product (e.g., fatty acid amide or ethanolamine) produced by the hydrolysis of a substrate such as oleoylethanolamide or anandamide. The substrate may be labeled to facilitate detection of the released reaction products. High throughput assays for the presence, absence, or quantification of particular reaction products are well known to those of skill in the art. Thus, for example, U.S. Pat. No. 5,559,410 discloses high throughput screening methods for proteins, and U.S. Pat. Nos. 5,576,220 and 5,541,061 disclose high throughput methods of screening for ligand/antibody binding.	B	8.11	pIC50	7.7	nM	IC50	US-9187413-B2. Peripherally restricted FAAH inhibitors (2015)
ChEMBL	Inhibition of FAAH in rat brain membranes assessed as hydrolysis of [14C]-anandamide preincubated for 20 mins before [14C]-anandamide addition measured after 30 mins	B	8.22	pIC50	6	nM	IC50	ACS Med Chem Lett (2012) 3: 182-186 [PMID:24900454]
ChEMBL	Inhibition of rat histidine tagged FAAH expressed in Escherichia coli by coumarin ester substrate fluorescence assay	B	8.31	pIC50	4.9	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4585-4592 [PMID:22738638]
ChEMBL	Inhibition of [3H]anandamide binding to fatty acid amide hydrolase of rat brain membranes	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2004) 47: 4998-5008 [PMID:15456244]
ChEMBL	Inhibition of rat brain membrane FAAH	B	8.34	pIC50	4.6	nM	IC50	Bioorg Med Chem (2012) 20: 1100-1112 [PMID:22196515]
ChEMBL	Inhibition of FAAH in rat brain membrane using anandamide[ethanolamine-3H] as substrate assessed as reduction in [3H]-ethanolamine production incubated for 30 mins by scintillation counting method	B	8.34	pIC50	4.6	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2701-2705 [PMID:27117424]
ChEMBL	Displacement of [3H]anandamide from FAAH in rat brain membranes after 30 mins by liquid scintillation counting	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2017) 60: 2287-2304 [PMID:28182408]
ChEMBL	Inhibition of FAAH in rat brain membrane assessed as [3H]anandamide hydrolysis	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2008) 51: 3487-3498 [PMID:18507372]
ChEMBL	Inhibition of FAAH in Wistar rat brain membranes using [3H]-anandamide as substrate preincubated for 10 mins followed by substrate addition measured after 4 mins by liquid scintillation counting method	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
ChEMBL	Inhibition of FAAH1 in Wistar rat brain homogenates using [3H]anandamide as substrate incubated for 30 mins by liquid scintillation counting method	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2018) 61: 7902-7916 [PMID:30126274]
ChEMBL	Inhibition of FAAH in Wistar rat brain membranes assessed as reduction in [3H]anandamide hydrolysis using [3H]anandamide as substrate preincubated for 10 mins followed by substrate addition and measured after 4 mins by HPLC/MS analysis	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (2019) 62: 10995-11003 [PMID:31407888]
ChEMBL	Displacement of [3H]-anandamide from rat brain FAAH after 30 mins by scintillation counting	B	8.34	pIC50	4.57	nM	IC50	Eur J Med Chem (2011) 46: 4466-4473 [PMID:21820769]
ChEMBL	Inhibition of FAAH in Wistar rat cerebral membrane by liquid scintillation counting	B	8.42	pIC50	3.8	nM	IC50	Eur J Med Chem (2009) 44: 2994-3008 [PMID:19232787]
ChEMBL	Inhibition of Wistar rat brain FAAH	B	8.42	pIC50	3.8	nM	IC50	J Med Chem (2006) 49: 4650-4656 [PMID:16854070]
ChEMBL	Inhibition of rat FAAH assessed as hydrolysis of anandamido-amino-methyl-cumarin	B	8.52	pIC50	3	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2492-2496 [PMID:21392988]
ChEMBL	Inhibition of rat FAAH expressed in HEK293-TRex cells using arachadonyl 7-amino 4-methyl coumarin amide as substrate by fluorescence assay	B	8.52	pIC50	3	nM	IC50	Medchemcomm (2012) 3: 1258-1263
ChEMBL	Inhibition of FAAH in Sprague-Dawley rat brain homogenates preincubated for 10 mins followed by addition of substrate measured after 30 mins by liquid scintillation counting	B	8.77	pIC50	1.7	nM	IC50	Eur J Med Chem (2016) 109: 216-237 [PMID:26774927]
ChEMBL	Inhibition of rat FAAH expressed in HEK293 cells using arachadonyl 7-amino 4-methyl coumarin amide as substrate by fluorimetric assay	B	9.15	pIC50	0.7	nM	IC50	Medchemcomm (2012) 3: 611-619
ChEMBL	Inhibition of FAAH in Wistar rat brain intact neurons assessed as reduction in [3H]anandamide hydrolysis using [3H]anandamide as substrate preincubated for 10 mins followed by substrate addition and measured after 4 mins by HPLC/MS analysis	B	9.3	pIC50	0.5	nM	IC50	J Med Chem (2019) 62: 10995-11003 [PMID:31407888]
ChEMBL	Inhibition of rat intact neuron FAAH	B	9.3	pIC50	0.5	nM	IC50	Bioorg Med Chem (2012) 20: 1100-1112 [PMID:22196515]
Cyclooxygenase-1 in Sheep (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2949] [UniProtKB: P05979]
ChEMBL	Inhibition of ovine COX1 assessed as production of PGF2-alpha preincubated with compound followed by the addition of 5 uM arachidonic acid as substrate by enzyme immunoassay	B	4	pIC50	>100000	nM	IC50	Eur J Med Chem (2016) 109: 216-237 [PMID:26774927]
COX-2 /Cyclooxygenase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354]
ChEMBL	Inhibition of recombinant human COX2 assessed as production of PGF2-alpha preincubated with compound followed by the addition of 5 uM arachidonic acid as substrate by enzyme immunoassay	B	4	pIC50	>100000	nM	IC50	Eur J Med Chem (2016) 109: 216-237 [PMID:26774927]
epoxide hydrolase 2/Epoxide hydratase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2409] [GtoPdb: 2970] [UniProtKB: P34913]
ChEMBL	Inhibition of human recombinant soluble epoxide hydrolase expressed in baculovirus-infected High Five cells S9 fraction using CMNPC substrate by fluorescence based assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2018) 28: 762-768 [PMID:29366648]
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7]
GtoPdb	-	-	8.3	pIC50	5.01	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]; Anesth Analg (2009) 108: 316-29 [PMID:19095868]
ChEMBL	Inhibition of recombinant human N-terminal FLAG-tagged/C-terminal Myc-His6 tagged FAAH2 (32 to 579 residues) expressed in COS7 cell membranes	B	8.3	pIC50	5	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762]
GtoPdb	Fluorometric Imaging Plate Reader (FLIRP) calcium-influx assay	-	4.6	pEC50	24000	nM	EC50	Mol Pharmacol (2007) 71: 1209-16 [PMID:17314320]
ChEMBL	Agonist activity at human TRPA1 channel expressed in HEK293 cells assessed as increase in intracellular calcium influx	F	4.61	pEC50	24500	nM	EC50	J Med Chem (2010) 53: 5085-5107 [PMID:20356305]
ChEMBL	Agonist activity at GDH-tagged human TRPA1 receptor expressed in HEK293F cells assessed as increase in intracellular Ca2+ concentration by FLIPR assay	B	4.61	pEC50	24500	nM	EC50	Eur J Med Chem (2013) 63: 118-132 [PMID:23474898]
ChEMBL	Agonist activity at human TRPA1 expressed in HEK293F cells assessed as increase in calcium influx by FLIPR analysis	B	4.61	pEC50	24500	nM	EC50	Eur J Med Chem (2019) 170: 141-156 [PMID:30878828]
ChEMBL	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	B	7	pEC50	100	nM	EC50	Eur J Med Chem (2019) 170: 141-156 [PMID:30878828]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]