ChEMBL ligand: CHEMBL917 (5-Fluoro-2-deoxyuridine, 5-fudr, Floxuridine, Fluoruridine deoxyribose, F.u.d.r., Fudr, FUDR, NSC-27640)
thymidylate synthetase/Thymidylate synthase in Mouse [ChEMBL: CHEMBL3160] [GtoPdb: 2642] [UniProtKB: P07607] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
thymidylate synthetase/Thymidylate synthase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3160] [GtoPdb: 2642] [UniProtKB: P07607]
ChEMBL	Inhibition of cellular thymidylate synthase activity of murine leukemia cell line (L1210) in permeabilised cells.	B	4.23	pIC50	59000	nM	IC50	J Med Chem (1996) 39: 4569-4575 [PMID:8917645]
ChEMBL	Inhibition of cellular thymidylate synthase activity of mouse fibroblast (L929 TK-) in permeabilised cells.	B	4.96	pIC50	11000	nM	IC50	J Med Chem (1996) 39: 4569-4575 [PMID:8917645]
ChEMBL	Inhibition of cellular thymidylate synthase activity of mouse fibroblast (L929 TK-) in intact cells.	B	5.14	pIC50	7300	nM	IC50	J Med Chem (1996) 39: 4569-4575 [PMID:8917645]
ChEMBL	Thymidylate synthase inhibition in thymidine kinase deficient LM cells after 2 hr treatment	B	5.27	pIC50	5400	nM	IC50	J Med Chem (2001) 44: 4475-4480 [PMID:11728193]
ChEMBL	Thymidylate synthase inhibition in wild type LM cells after 2 hr treatment	B	6.59	pIC50	260	nM	IC50	J Med Chem (2001) 44: 4475-4480 [PMID:11728193]
ChEMBL	Inhibition of cellular thymidylate synthase activity of murine leukemia cell line (L1210) in intact cells.	B	7.37	pIC50	43	nM	IC50	J Med Chem (1996) 39: 4569-4575 [PMID:8917645]
ChEMBL	Thymidylate synthase inhibition in thymidine kinase deficient /TK cells after 2 hr treatment	B	7.7	pIC50	20	nM	IC50	J Med Chem (2001) 44: 4475-4480 [PMID:11728193]
ChEMBL	Thymidylate synthase inhibition in L1210 mouse leukemia cells after 2 hr treatment	B	8.1	pIC50	7.9	nM	IC50	J Med Chem (2001) 44: 4475-4480 [PMID:11728193]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxycytidine after preincubation for 4 hrs by liquid scintillation counting	B	9.15	pIC50	0.7	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxycytidine after preincubation for 24 hrs by liquid scintillation counting	B	9.15	pIC50	0.7	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxyuridine after preincubation for 15 mins by liquid scintillation counting	B	9.22	pIC50	0.6	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxycytidine after preincubation for 15 mins by liquid scintillation counting	B	9.22	pIC50	0.6	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxyuridine after preincubation for 24 hrs by liquid scintillation counting	B	9.4	pIC50	0.4	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]
ChEMBL	Inhibition of thymidylate synthase in mouse L1210 cells assessed as inhibition of tritium release from [5-3H]deoxyuridine after preincubation for 4 hrs by liquid scintillation counting	B	9.4	pIC50	0.4	nM	IC50	Eur J Med Chem (2011) 46: 993-1005 [PMID:21330014]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]