ChEMBL ligand: CHEMBL34259 (Abitrexate, ADX-2191, Amethopterin, CL 14377, CL-14377, D-amethopterin, D-methotrexate, Ebetrex, EMT-25299, Emtexate high-pot, Emtexate pf, Folex, Jylamvo, Maxtrex, Methotrexate, Methotrexate, d-, Methotrexate, (r)-, Methotrexatum, Methylaminopterin, Metoject, Mexate, Mexate-AQ, Nordimet, NSC-740, Otrexup, Otrexup pfs, R-9985, Rasuvo, Reditrex, Rheumatrex, (r)-methotrexate, R-methotrexate, TCMDC-123832, TCMDC-125488, TCMDC-125858, Trexall, Zlatal)
AICAR transformylase in Human [ChEMBL: CHEMBL2518] [UniProtKB: P31939] There should be some charts here, you may need to enable JavaScript!
Bifunctional dihydrofolate reductase-thymidylate synthase in Trypanosoma cruzi [ChEMBL: CHEMBL1163130] [UniProtKB: Q27793] There should be some charts here, you may need to enable JavaScript!
ABCC3/Canalicular multispecific organic anion transporter 2 in Rat [ChEMBL: CHEMBL2073682] [GtoPdb: 781] [UniProtKB: O88563] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4] Dihydrofolate reductase in S.aureus [ChEMBL: CHEMBL1681620] [UniProtKB: P0A017] Dihydrofolate reductase in Enterococcus faecium [ChEMBL: CHEMBL3243913] [UniProtKB: P00380] Dihydrofolate reductase in Bacillus anthracis [ChEMBL: CHEMBL5270] [UniProtKB: Q81R22] Dihydrofolate reductase in Lactobacillus casei [ChEMBL: CHEMBL2902] [UniProtKB: P00381] Dihydrofolate reductase in Mycobacterium tuberculosis [ChEMBL: CHEMBL6065] [UniProtKB: P9WNX1] Dihydrofolate reductase in Pneumocystis carinii [ChEMBL: CHEMBL1926] [UniProtKB: P16184] Dihydrofolate reductase in Leishmania major [ChEMBL: CHEMBL4614] [UniProtKB: P07382] Dihydrofolate reductase in Toxoplasma gondii [ChEMBL: CHEMBL2425] [UniProtKB: Q07422] Dihydrofolate reductase in Plasmodium falciparum K1 [ChEMBL: CHEMBL1939] [UniProtKB: P13922] Dihydrofolate reductase in Chicken [ChEMBL: CHEMBL2575] [UniProtKB: P00378] dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] Dihydrofolate reductase in Bovine [ChEMBL: CHEMBL1075051] [UniProtKB: P00376] dihydrofolate reductase/Dihydrofolate reductase in Mouse [ChEMBL: CHEMBL4564] [GtoPdb: 2603] [UniProtKB: P00375] dihydrofolate reductase/Dihydrofolate reductase in Rat [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2] There should be some charts here, you may need to enable JavaScript!
folate receptor alpha/Folate receptor alpha in Human [ChEMBL: CHEMBL2121] [GtoPdb: 3212] [UniProtKB: P15328] There should be some charts here, you may need to enable JavaScript!
Folate receptor beta in Human [ChEMBL: CHEMBL5064] [UniProtKB: P14207] There should be some charts here, you may need to enable JavaScript!
Reduced folate transporter 1/Folate transporter 1 in Human [ChEMBL: CHEMBL4833] [GtoPdb: 1014] [UniProtKB: P41440] There should be some charts here, you may need to enable JavaScript!
Folylpoly-gamma-glutamate synthetase in Human [ChEMBL: CHEMBL3171] [UniProtKB: Q05932] There should be some charts here, you may need to enable JavaScript!
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase/GAR transformylase in Human [ChEMBL: CHEMBL3972] [GtoPdb: 2612] [UniProtKB: P22102] phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase/GAR transformylase in Mouse [ChEMBL: CHEMBL3690] [GtoPdb: 2612] [UniProtKB: Q64737] There should be some charts here, you may need to enable JavaScript!
high mobility group box 1/High mobility group protein B1 in Human [ChEMBL: CHEMBL2311236] [GtoPdb: 3279] [UniProtKB: P09429] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Proton-coupled folate transporter in Human [ChEMBL: CHEMBL1795188] [GtoPdb: 1213] [UniProtKB: Q96NT5] There should be some charts here, you may need to enable JavaScript!
Pteridine reductase 1 in Leishmania major [ChEMBL: CHEMBL6194] [UniProtKB: Q01782] There should be some charts here, you may need to enable JavaScript!
Thymidylate synthase in Escherichia coli [ChEMBL: CHEMBL6137] [UniProtKB: C3SWJ7] Thymidylate synthase in Lactobacillus casei [ChEMBL: CHEMBL5328] [UniProtKB: P00469] thymidylate synthetase/Thymidylate synthase in Human [ChEMBL: CHEMBL1952] [GtoPdb: 2642] [UniProtKB: P04818] thymidylate synthetase/Thymidylate synthase in Mouse [ChEMBL: CHEMBL3160] [GtoPdb: 2642] [UniProtKB: P07607] There should be some charts here, you may need to enable JavaScript!
Thymidylate synthase (EC 2.1.1.45) (TS) (TSase) in Escherichia coli [ChEMBL: CHEMBL1795153] [UniProtKB: E2QDN0] There should be some charts here, you may need to enable JavaScript!
TLR4/Toll-like receptor 4 in Human [ChEMBL: CHEMBL5255] [GtoPdb: 1754] [UniProtKB: O00206] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
AICAR transformylase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2518] [UniProtKB: P31939]
ChEMBL	Inhibition of human full length N-terminal His-tagged ATIC expressed in Chinese Hamster MTXRII-OuaR2-4 R2 cells assessed as reduction in THF formation using 10-CHOTHF as substrate by spectrophotometric method	B	6.06	pKi	880	nM	Ki	Bioorg Med Chem (2021) 37: 116093-116093 [PMID:33773393]
ChEMBL	Inhibitory activity against AICAR formyltransferase of Lactobacillus casei	B	4.7	pIC50	>20000	nM	IC50	J Med Chem (1988) 31: 150-153 [PMID:3121855]
Bifunctional dihydrofolate reductase-thymidylate synthase in Trypanosoma cruzi (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1163130] [UniProtKB: Q27793]
ChEMBL	Inhibition of Trypanosoma cruzi DHFR-TS in presence of NADPH by spectrophotometry	B	6.96	pIC50	110	nM	IC50	Bioorg Med Chem (2022) 58: 116577-116577 [PMID:35189560]
ABCC3/Canalicular multispecific organic anion transporter 2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073682] [GtoPdb: 781] [UniProtKB: O88563]
ChEMBL	TP_TRANSPORTER: inhibition of E217betaG uptake in membrane vesicles from Mrp3-expressing Sf9 cells	F	4.05	pKi	90000	nM	Ki	Pharm Res (2002) 19: 34-41 [PMID:11837698]
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4]
ChEMBL	Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 9.5	B	5.77	pKd	1700	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 9.5	B	5.96	pKd	1100	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 8	B	6.85	pKd	140	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 8	B	7.08	pKd	84	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 9.5	B	7.51	pKd	31	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 6	B	8.49	pKd	3.2	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 6	B	8.64	pKd	2.3	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Inhibitory activity against dihydrofolate reductase	B	9	pKd	<1	nM	Kd	Bioorg Med Chem Lett (2004) 14: 2285-2289 [PMID:15081026]
ChEMBL	Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 8	B	10.02	pKd	0.1	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 6	B	10.64	pKd	0.02	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Binding constant(Ki) to dihydrofolate reductase (DHFR) Tyr -31 was determined	B	5.62	pKi	2400	nM	Ki	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Binding constant(Ki) to dihydrofolate reductase (DHFR) Phe -31 was determined	B	6.48	pKi	330	nM	Ki	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Binding affinity against Dihydrofolate reductase of Escherichia coli	B	10.68	pKi	0.02	nM	Ki	J Med Chem (1985) 28: 303-311 [PMID:3973902]
ChEMBL	Inhibitory activity against recombinant Escherichia coli dihydrofolate reductase	B	5.36	pIC50	4400	nM	IC50	J Med Chem (2001) 44: 1993-2003 [PMID:11384244]
ChEMBL	Inhibition of Escherichia coli DHFR	B	7.49	pIC50	32	nM	IC50	J Med Chem (2004) 47: 6730-6739 [PMID:15615522]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase (DHFR)	B	8.05	pIC50	9	nM	IC50	J Med Chem (2002) 45: 1942-1948 [PMID:11960504]
ChEMBL	Inhibitory concentration against Escherichia coli Dihydrofolate reductase	B	8.05	pIC50	9	nM	IC50	J Med Chem (2000) 43: 3125-3133 [PMID:10956221]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.06	pIC50	8.8	nM	IC50	J Med Chem (2008) 51: 5789-5797 [PMID:18800768]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.06	pIC50	8.8	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	8.06	pIC50	8.8	nM	IC50	J Med Chem (2009) 52: 4892-4902 [PMID:19719239]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.15	pIC50	7	nM	IC50	J Med Chem (2004) 47: 6893-6901 [PMID:15615538]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase at 37 degree C pH 7.4	B	8.15	pIC50	7	nM	IC50	J Med Chem (2005) 48: 5329-5336 [PMID:16078850]
ChEMBL	Compound was evaluated as inhibitor of Escherichia coli Dihydrofolate reductase	B	8.15	pIC50	7	nM	IC50	J Med Chem (2003) 46: 591-600 [PMID:12570380]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.18	pIC50	6.6	nM	IC50	J Med Chem (2005) 48: 7215-7222 [PMID:16279780]
ChEMBL	Inhibition of Escherichia coli DHFR at 30 degC under pH 7.4 by spectrophotometry	B	8.18	pIC50	6.6	nM	IC50	Bioorg Med Chem (2010) 18: 953-961 [PMID:20056546]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.18	pIC50	6.6	nM	IC50	J Med Chem (2010) 53: 1563-1578 [PMID:20092323]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	8.18	pIC50	6.6	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibitory activity against Escherichia coli (ec) Dihydrofolate reductase	B	8.22	pIC50	6	nM	IC50	J Med Chem (2002) 45: 5173-5181 [PMID:12408727]
ChEMBL	Inhibitory concentration against isolated Escherichia coli Dihydrofolate reductase	B	8.22	pIC50	6	nM	IC50	J Med Chem (2000) 43: 3837-3851 [PMID:11052789]
ChEMBL	Inhibition of Escherichia coli DHFR by spectrophotometry	B	8.36	pIC50	4.4	nM	IC50	Bioorg Med Chem (2011) 19: 3585-3594 [PMID:21550809]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8.52	pIC50	3	nM	IC50	J Med Chem (2006) 49: 6977-6986 [PMID:17125251]
ChEMBL	Inhibitory activity against recombinant ec dihydrofolate reductase(in 50 uM dihydrofolic acid)	B	8.66	pIC50	2.2	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]
Dihydrofolate reductase in S.aureus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1681620] [UniProtKB: P0A017]
ChEMBL	Inhibition of Staphylococcus aureus DHFR	B	9	pKi	1	nM	Ki	ACS Med Chem Lett (2016) 7: 692-696 [PMID:27437079]
Dihydrofolate reductase in Enterococcus faecium (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3243913] [UniProtKB: P00380]
ChEMBL	Inhibition of Streptococcus faecium dihydrofolate reductase using dihydrofolate as substrate	B	8.85	pIC50	1.4	nM	IC50	J Med Chem (1977) 20: 588-591 [PMID:850245]
Dihydrofolate reductase in Bacillus anthracis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5270] [UniProtKB: Q81R22]
ChEMBL	Inhibition of Bacillus anthracis DHFR	B	7.82	pIC50	15	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3065-3073 [PMID:19364848]
ChEMBL	Inhibition of Bacillus anthracis DHFR	B	7.91	pIC50	12.2	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3065-3073 [PMID:19364848]
Dihydrofolate reductase in Lactobacillus casei (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2902] [UniProtKB: P00381]
ChEMBL	Inhibitory activity against recombinant lc dihydrofolate reductase(in 50 uM dihydrofolic acid)	B	7.26	pIC50	55	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]
ChEMBL	Inhibitory concentration against isolated Lactobacillus casei Dihydrofolate reductase	B	7.36	pIC50	44	nM	IC50	J Med Chem (2000) 43: 3837-3851 [PMID:11052789]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 2)	F	7.59	pIC50	26	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	Compound was evaluated as inhibitor of Lactobacillus casei Dihydrofolate reductase	B	7.66	pIC50	22	nM	IC50	J Med Chem (2003) 46: 591-600 [PMID:12570380]
ChEMBL	Inhibition of Lactobacillus casei DHFR	B	7.66	pIC50	22	nM	IC50	J Med Chem (2004) 47: 6893-6901 [PMID:15615538]
ChEMBL	Inhibition of Lactobacillus casei dihydrofolate reductase at 37 degree C pH 7.4	B	7.66	pIC50	22	nM	IC50	J Med Chem (2005) 48: 5329-5336 [PMID:16078850]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 6)	F	7.8	pIC50	16	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 3)	F	7.8	pIC50	16	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	Evaluated for inhibition of dihydrofolate reductase (DHFR) from Lactobacillus casei	B	7.8	pIC50	16	nM	IC50	J Med Chem (1988) 31: 2164-2169 [PMID:3184124]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 2)	F	7.8	pIC50	16	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	Inhibitory activity against dihydrofolate reductase of Lactobacillus casei	B	7.8	pIC50	16	nM	IC50	J Med Chem (1988) 31: 150-153 [PMID:3121855]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 5)	F	7.82	pIC50	15	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 4)	F	7.82	pIC50	15	nM	IC50	J Med Chem (1988) 31: 181-185 [PMID:2447278]
ChEMBL	In vitro inhibition of Dihydrofolate reductase activity against Lactobacillus casei enzyme	F	7.92	pIC50	12	nM	IC50	J Med Chem (1982) 25: 877-880 [PMID:7108907]
ChEMBL	Inhibitory concentration against Lactobacillus casei Dihydrofolate reductase	B	7.96	pIC50	11	nM	IC50	J Med Chem (2000) 43: 3125-3133 [PMID:10956221]
ChEMBL	Compound was evaluated for the inhibition of dihydrofolate reductase (DHFR) derived from Lactobacillus casei ATCC 7469	B	8	pIC50	10	nM	IC50	J Med Chem (1986) 29: 1056-1061 [PMID:3712374]
ChEMBL	Compound was evaluated for the inhibition of dihydrofolate reductase (DHFR)derived from Lactobacillus casei	B	8	pIC50	10	nM	IC50	J Med Chem (1986) 29: 1786-1789 [PMID:3091834]
ChEMBL	Ability to inhibit Lactobacillus casei dihydrofolate reductase in vitro was determined	B	8.02	pIC50	9.6	nM	IC50	J Med Chem (1980) 23: 899-903 [PMID:6772788]
ChEMBL	Inhibitory activity against dihydrofolate reductase of Lactobacillus casei	B	8.07	pIC50	8.5	nM	IC50	J Med Chem (1986) 29: 1754-1760 [PMID:3091832]
ChEMBL	Concentration required for 50% inhibition against dihydrofolate reductase of Lactobacillus casei	B	8.1	pIC50	8	nM	IC50	J Med Chem (1983) 26: 605-607 [PMID:6403710]
ChEMBL	Inhibitory activity against dihydrofolate reductase derived from Lactobacillus casei	B	8.1	pIC50	8	nM	IC50	J Med Chem (1986) 29: 1263-1269 [PMID:3100797]
ChEMBL	inhibitory concentration against Lactobacillus casei DHFR(Dihydro folate reductase).	B	8.22	pIC50	6	nM	IC50	J Med Chem (1995) 38: 3798-3805 [PMID:7562910]
ChEMBL	Inhibitory concentration for DHFR in Lactobacillus casei	B	8.22	pIC50	6	nM	IC50	J Med Chem (1994) 37: 1169-1176 [PMID:8164259]
ChEMBL	Inhibition of Lactobacillus casei DHFR	B	8.22	pIC50	6	nM	IC50	J Med Chem (2007) 50: 3283-3289 [PMID:17569517]
ChEMBL	Inhibition of Lactobacillus casei DHFR by spectrophotometric analysis	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem (2010) 18: 5081-5089 [PMID:20580561]
ChEMBL	Inhibition of DCM-resistant Lactobacillus casei DHFR	B	8.35	pIC50	4.5	nM	IC50	J Med Chem (1976) 19: 825-829 [PMID:820858]
ChEMBL	Inhibition of MTX-resistant Lactobacillus casei dihydrofolate reductase	B	8.52	pIC50	3	nM	IC50	J Med Chem (1977) 20: 1323-1327 [PMID:409842]
ChEMBL	Inhibition of Lactobacillus casei ATCC 7469 dihydrofolate reductase	B	8.52	pIC50	3	nM	IC50	J Med Chem (1979) 22: 869-874 [PMID:109616]
ChEMBL	Inhibitory activity against recombinant sf dihydrofolate reductase(in 50 uM dihydrofolic acid)	B	8.66	pIC50	2.2	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]
ChEMBL	Compound was tested for the inhibition of dihydrofolate reductase in Bovine liver	B	8.89	pIC50	1.3	nM	IC50	J Med Chem (1994) 37: 1616-1624 [PMID:8201595]
Dihydrofolate reductase in Mycobacterium tuberculosis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6065] [UniProtKB: P9WNX1]
ChEMBL	Inhibition of Mycobacterium tuberculosis His-tagged DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs over 6 mins by spectrophotometric method	B	7.46	pIC50	35	nM	IC50	Eur J Med Chem (2018) 144: 262-276 [PMID:29274493]
ChEMBL	Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and measured for 2 mins by spectrophotometric method	B	8.06	pIC50	8.8	nM	IC50	Bioorg Med Chem (2019) 27: 1421-1429 [PMID:30827867]
ChEMBL	Inhibition of recombinant Mycobacterium tuberculosis DHFR using 45 uM DHF as substrate by spectrophotometry	B	8.08	pIC50	8.25	nM	IC50	ACS Med Chem Lett (2015) 6: 1140-1144 [PMID:26617968]
Dihydrofolate reductase in Pneumocystis carinii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1926] [UniProtKB: P16184]
ChEMBL	Inhibitory activity was determined against dihydrofolate reductase in Pneumocystis carinii.	B	6.06	pIC50	880	nM	IC50	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibitory concentration against isolated Pneumocystis carinii DHFR (Dihydrofolate reductase)	B	7.86	pIC50	13.8	nM	IC50	J Med Chem (2000) 43: 3837-3851 [PMID:11052789]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Pneumocystis carinii at 37 centigrade.	B	8.89	pIC50	1.3	nM	IC50	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Pneumocystis carinii was evaluated using 90 uM dihydrofolic acid as substrate	B	8.89	pIC50	1.3	nM	IC50	J Med Chem (1997) 40: 3040-3048 [PMID:9301666]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase (in 90 uM dihydrofolic acid)	B	8.89	pIC50	1.3	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]