topotecan [Ligand Id: 7101] activity data from GtoPdb and ChEMBL
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| ChEMBL ligand: CHEMBL84 (Ff-10850 (liposomal topotecan), Hycamptin, NSC-609699, NSC-641007, SKF-104864, Topotecan, Topotecane) |
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| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| ABCG2/ATP-binding cassette sub-family G member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0] | ||||||||
| ChEMBL | TP_TRANSPORTER: drug resistance(Imatinib mesylate) in BCRP-expressing SaoS2 cells | F | 6.77 | pIC50 | <170 | nM | IC50 | Cancer Res (2004) 64: 2333-2337 [PMID:15059881] |
| CYP3A4/Cytochrome P450 3A4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL340] [GtoPdb: 1337] [UniProtKB: P08684] | ||||||||
| ChEMBL | DRUGMATRIX: CYP450, 3A4 enzyme inhibition (substrate: 7-Benzyloxy-4-(trifluoromethyl)-coumarin) | B | 5.82 | pIC50 | 1521.1 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| DNA topoisomerase I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1781] [GtoPdb: 2636] [UniProtKB: P11387] | ||||||||
| ChEMBL | Binding affinity to topoisomerase 1 | B | 7.58 | pKd | 26.5 | nM | Kd | J Nat Prod (2011) 74: 1401-1407 [PMID:21542600] |
| ChEMBL | Inhibition of human topoisomerase I using DNA pBR322 as substrate assessed as DNA relaxation incubated for 45 mins by ethidium bromide staining based agarose gel electrophoresis | B | 4.64 | pIC50 | 22880 | nM | IC50 | Eur J Med Chem (2021) 226: 113817-113817 [PMID:34537445] |
| ChEMBL | Inhibition of DNA topoisomerase 1 in Homo sapiens (human) SW480 cells | B | 5.02 | pIC50 | 9650 | nM | IC50 | Med Chem Res (2010) 19: 817-835 |
| ChEMBL | Inhibition of DNA topoisomerase 1 in Homo sapiens (human) HCT116 cells | B | 5.26 | pIC50 | 5550 | nM | IC50 | Med Chem Res (2010) 19: 817-835 |
| ChEMBL | Inhibition of DNA topoisomerase 1 in Homo sapiens (human) VACO 241 cells | B | 5.28 | pIC50 | 5250 | nM | IC50 | Med Chem Res (2010) 19: 817-835 |
| ChEMBL | Inhibition of human topoisomerase 1 | B | 5.49 | pIC50 | 3200 | nM | IC50 | Eur J Med Chem (2021) 220: 113555-113555 [PMID:34052677] |
| ChEMBL | Inhibition of Top1 in human MCF7 cells using supercoiled pBS (SK+) DNA as substrate incubated for 30 mins by ethidium bromide staining based agarose gel electrophoresis analysis | B | 5.74 | pIC50 | 1800 | nM | IC50 | Eur J Med Chem (2020) 202: 112551-112551 [PMID:32682183] |
| ChEMBL | Poison activity at TOP1 in human MCF7 cells assessed as decrease in relaxed supercoiled pBS(SK+) DNA mobility measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis | B | 5.74 | pIC50 | 1800 | nM | IC50 | J Med Chem (2019) 62: 3428-3446 [PMID:30897325] |
| ChEMBL | Average concentration of compound to cause 50% inhibition of topoisomerase-1 isolated from calf thymus | B | 5.96 | pIC50 | 1100 | nM | IC50 | J Med Chem (1996) 39: 713-719 [PMID:8576914] |
| ChEMBL | Inhibition of topoisomerase I activity was determined in vitro by using the cleavable complex assay(calf thymus) | B | 5.99 | pIC50 | 1028 | nM | IC50 | J Med Chem (1995) 38: 395-401 [PMID:7853331] |
| ChEMBL | Inhibition of Topoisomerase I by cleavage complex formation in human HL-60 cells | B | 6 | pIC50 | 1000 | nM | IC50 | J Med Chem (1993) 36: 2689-2700 [PMID:8410981] |
| ChEMBL | Inhibition of topoisomerase I-DNA complex in trapping assay | B | 7.3 | pIC50 | 50 | nM | IC50 | J Med Chem (2005) 48: 2336-2345 [PMID:15801827] |
| ChEMBL | Inhibition of recombinant human Top1 expressed in baculovirus infected sf9 insect cells using supercoiled pBS (SK+) DNA as substrate incubated for 30 mins by ethidium bromide staining based agarose gel electrophoresis analysis | B | 7.68 | pIC50 | 21 | nM | IC50 | Eur J Med Chem (2020) 202: 112551-112551 [PMID:32682183] |
| ChEMBL | Poison activity at recombinant human TOP1 expressed in baculovirus infected Sf9 insect cells assessed as decrease in relaxed supercoiled pBS(SK+) DNA mobility measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis | B | 7.68 | pIC50 | 21 | nM | IC50 | J Med Chem (2019) 62: 3428-3446 [PMID:30897325] |
| ChEMBL | Inhibition of topoisomerase-1 in human U251 cells assessed as inhibition of HIF-1-mediated hypoxia-induced VEGF expression after 24 hrs by luciferase reporter gene assay | B | 7.15 | pEC50 | 71.3 | nM | EC50 | Eur J Med Chem (2012) 49: 24-40 [PMID:22305612] |
| ChEMBL | Inhibition of topoisomerase-1 in human U251 cells assessed as inhibition of hypoxia-induced HIF-1alpha accumulation in nuclear extract after 6 to 24 hrs by immunoblot analysis | B | 7.27 | pEC50 | 54 | nM | EC50 | Eur J Med Chem (2012) 49: 24-40 [PMID:22305612] |
| DNA topoisomerase I in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2814] [GtoPdb: 2636] [UniProtKB: Q04750] | ||||||||
| ChEMBL | Average concentration to cause 50% inhibition of topo 1 using the cleavable complex assay | B | 4.7 | pIC50 | 20000 | nM | IC50 | J Med Chem (2001) 44: 1594-1602 [PMID:11334569] |
| Hypoxia-inducible factor 1 alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4261] [UniProtKB: Q16665] | ||||||||
| ChEMBL | Inhibition of HIF1alpha in human U251 cells under normoxic condition by luciferase reporter gene assay | B | 5.52 | pIC50 | >3000 | nM | IC50 | Bioorg Med Chem Lett (2010) 20: 6426-6429 [PMID:20932758] |
| ChEMBL | Inhibition of HIF1alpha in human U251 cells under hypoxic condition by luciferase reporter gene assay | B | 7.22 | pIC50 | 60 | nM | IC50 | Bioorg Med Chem Lett (2010) 20: 6426-6429 [PMID:20932758] |
| ChEMBL | Inhibition of human HIF1alpha-mediated transcription expressed in human U251 cells co-transfected with pGL2-TK-HRE incubated for 24 hrs by luciferase reporter gene assay | B | 7.92 | pEC50 | 12 | nM | EC50 | Medchemcomm (2014) 5: 923-926 |
| Multidrug and toxin extrusion/Multidrug and toxin extrusion protein 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1743126] [GtoPdb: 1216] [UniProtKB: Q96FL8] | ||||||||
| ChEMBL | Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay | B | 5.89 | pIC50 | 1300 | nM | IC50 | J Med Chem (2013) 56: 781-795 [PMID:23241029] |
| MATE2/Multidrug and toxin extrusion protein 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1743127] [GtoPdb: 1217] [UniProtKB: Q86VL8] | ||||||||
| ChEMBL | Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay | B | 5.07 | pIC50 | 8600 | nM | IC50 | J Med Chem (2013) 56: 781-795 [PMID:23241029] |
| Organic cation transporter 2/Solute carrier family 22 member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1743122] [GtoPdb: 1020] [UniProtKB: O15244] | ||||||||
| ChEMBL | Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay | B | 4.21 | pIC50 | 61000 | nM | IC50 | J Med Chem (2013) 56: 781-795 [PMID:23241029] |
ChEMBL data shown on this page come from version 34:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
