ChEMBL ligand: CHEMBL3109630
euchromatic histone lysine methyltransferase 2/Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 in Human [ChEMBL: CHEMBL6032] [GtoPdb: 2652] [UniProtKB: Q96KQ7] There should be some charts here, you may need to enable JavaScript!
euchromatic histone lysine methyltransferase 1/Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 in Human [ChEMBL: CHEMBL6031] [GtoPdb: 2651] [UniProtKB: Q9H9B1] There should be some charts here, you may need to enable JavaScript!
Spindlin-1 in Human [ChEMBL: CHEMBL4523509] [UniProtKB: Q9Y657] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
euchromatic histone lysine methyltransferase 2/Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6032] [GtoPdb: 2652] [UniProtKB: Q96KQ7]
ChEMBL	Inhibition of N-terminal GST tagged human recombinant EHMT2 (785 to 1210 residues) expressed in baculovirus infected Sf9 insect cells assessed as reduction in conversion of SAH to AMP using histone H3 as substrate and SAH as cosubstrate incubated for 30 mins by AMP Glo detection assay	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2516-2524 [PMID:31350126]
GtoPdb	-	-	8.48	pIC50	3.3	nM	IC50	A-366: a chemical Probe for G9a/GLP.. thesgc.org; ACS Med Chem Lett (2014) 5: 205-9 [PMID:24900801]
ChEMBL	Inhibition of G9a (unknown origin) using biotinylated-histone H3(1-21) peptide as substrate after 3 hrs by AlphaLISA assay	B	8.48	pIC50	3.3	nM	IC50	ACS Med Chem Lett (2014) 5: 205-209 [PMID:24900801]
ChEMBL	Inhibition of G9a (unknown origin)	B	8.48	pIC50	3.3	nM	IC50	J Med Chem (2015) 58: 2569-2583 [PMID:25406944]
ChEMBL	Inhibition of G9a (unknown origin)	B	8.48	pIC50	3.3	nM	IC50	Bioorg Med Chem (2016) 24: 6102-6108 [PMID:27720557]
ChEMBL	Inhibition of G9a (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate by scintillation proximity assay	B	8.52	pIC50	3	nM	IC50	ACS Med Chem Lett (2014) 5: 205-209 [PMID:24900801]
ChEMBL	Inhibition of G9a (unknown origin)	B	8.52	pIC50	3	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
euchromatic histone lysine methyltransferase 1/Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6031] [GtoPdb: 2651] [UniProtKB: Q9H9B1]
ChEMBL	Inhibition of GLP (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate by scintillation proximity assay	B	7.42	pIC50	38	nM	IC50	ACS Med Chem Lett (2014) 5: 205-209 [PMID:24900801]
ChEMBL	Inhibition of N-terminal GST tagged human recombinant EHMT1 (794 to 1294 residues) expressed in baculovirus infected Sf9 insect cells assessed as reduction in conversion of SAH to AMP using histone H3 as substrate and SAH as cosubstrate incubated for 30 mins by AMP Glo detection assay	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2516-2524 [PMID:31350126]
GtoPdb	-	-	8.52	pIC50	38	nM	IC50	A-366: a chemical Probe for G9a/GLP.. thesgc.org; ACS Med Chem Lett (2014) 5: 205-9 [PMID:24900801]
Spindlin-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523509] [UniProtKB: Q9Y657]
ChEMBL	Binding affinity to recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry	B	6.41	pKd	393	nM	Kd	J Med Chem (2019) 62: 9008-9025 [PMID:31550156]
ChEMBL	Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry	B	6.74	pKd	180	nM	Kd	J Med Chem (2019) 62: 9008-9025 [PMID:31550156]
ChEMBL	Inhibition of H3(1-12)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by FTSA assay	B	6.96	pKd	110	nM	Kd	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method	B	5.82	pIC50	1500	nM	IC50	J Med Chem (2019) 62: 9008-9025 [PMID:31550156]
ChEMBL	Inhibition of FL-H3K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 30 mins by fluorescence polarization displacement assay	B	6.68	pIC50	207	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by AlphaLISA assay	B	6.73	pIC50	186	nM	IC50	J Med Chem (2019) 62: 9008-9025 [PMID:31550156]
ChEMBL	Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay	B	7.14	pIC50	72	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]