L-NNA [Ligand Id: 8759] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL227744 (L-Nitroarginine, L-N-Nitroarginine, L-Nw-Nitroarginine)
  • Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
  • Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
  • Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
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  • Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
  • Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
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  • Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
  • Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
  • Inducible NOS/Nitric oxide synthase, inducible in Rat [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL Inhibition of nNOS assessed as conversion of L-[3H]arginine to L-[3H]citrulline B 6.3 pKi 500 nM Ki Bioorg Med Chem (2008) 16: 6193-6206 [PMID:18477512]
GtoPdb - - 7.82 pKi 15 nM Ki J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme. B 7.82 pKi 15 nM Ki J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of human nNOS by hemoglobin capture assay B 5.36 pIC50 4400 nM IC50 J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase B 6.3 pIC50 500 nM IC50 J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS B 7.1 pIC50 80 nM IC50 Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL Concentration required to inhibit neuronal nitric oxide synthase B 7.1 pIC50 80 nM IC50 Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL Inhibition of neuronal nitric oxide synthase (nNOS) in mice B 6.3 pIC50 500 nM IC50 Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL Inhibition of rat recombinant nNOS B 5.34 pKi 4550 nM Ki J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of wild type rat nNOS by hemoglobin capture assay B 5.22 pIC50 6000 nM IC50 J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibition of recombinant rat nNOS expressed in Escherichia coli by hemoglobin capture assay B 5.3 pIC50 5000 nM IC50 Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay B 5.3 pIC50 5000 nM IC50 Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL Inhibition of rat nNOS L337H mutant by hemoglobin capture assay B 5.4 pIC50 4000 nM IC50 J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay B 6.22 pIC50 600 nM IC50 J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons) B 6.22 pIC50 600 nM IC50 Bioorg Med Chem Lett (1996) 6: 999-1002
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
GtoPdb - - 7.52 pKi 30 nM Ki J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme. B 7.52 pKi 30 nM Ki J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS) B 6.3 pIC50 500 nM IC50 J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against recombinant human Endothelial nitric oxide synthase B 6.49 pIC50 320 nM IC50 Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL In vitro inhibition of endothelial nitric oxide synthase. B 6.49 pIC50 320 nM IC50 Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL Inhibition of bovine recombinant eNOS B 6.12 pKi 750 nM Ki J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of bovine recombinant endothelial NOS by hemoglobin capture assay B 6.14 pKi 720 nM Ki J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells B 5.92 pIC50 1200 nM IC50 J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
GtoPdb - - 6.17 pKi 670 nM Ki J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme. B 6.17 pKi 670 nM Ki J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS) B 5.12 pIC50 7600 nM IC50 J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against human Inducible nitric oxide synthase B 5.26 pIC50 5500 nM IC50 Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL In vitro inhibition of inducible nitric oxide synthase. B 5.26 pIC50 5500 nM IC50 Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL Inhibition of mouse recombinant inducible NOS by hemoglobin capture assay B 5.37 pKi 4280 nM Ki J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL Inhibition of mouse recombinant iNOS B 6.24 pKi 570 nM Ki J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of inducible nitric oxide synthase (iNOS) in mice B 4.1 pIC50 80000 nM IC50 Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
ChEMBL Inhibition of iNOS in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction incubated for 24 hrs by Griess assay B 4.56 pIC50 27550 nM IC50 Bioorg Med Chem (2020) 28: 115733-115733 [PMID:33065432]
ChEMBL Inhibition of inducible NOS mediated nitric oxide production in LPS-activated Kunming mouse macrophages B 4.83 pIC50 14740 nM IC50 Bioorg Med Chem (2008) 16: 578-585 [PMID:17937988]
ChEMBL Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells B 4.85 pIC50 14000 nM IC50 J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase B 5 pIC50 10000 nM IC50 Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL Tested for inhibition of mouse inducible nitric oxide synthase B 6.3 pIC50 500 nM IC50 J Med Chem (1994) 37: 3886-3888 [PMID:7525961]
Inducible NOS/Nitric oxide synthase, inducible in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
ChEMBL Tested for inhibition of rat brain inducible nitric oxide synthase B 4.7 pIC50 20000 nM IC50 J Med Chem (1994) 37: 3886-3888 [PMID:7525961]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]