ChEMBL ligand: CHEMBL227744 (L-Nitroarginine, L-N-Nitroarginine, L-Nw-Nitroarginine)
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] Inducible NOS/Nitric oxide synthase, inducible in Rat [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Inhibition of nNOS assessed as conversion of L-[3H]arginine to L-[3H]citrulline	B	6.3	pKi	500	nM	Ki	Bioorg Med Chem (2008) 16: 6193-6206 [PMID:18477512]
GtoPdb	-	-	7.82	pKi	15	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme.	B	7.82	pKi	15	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of human nNOS by hemoglobin capture assay	B	5.36	pIC50	4400	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase	B	6.3	pIC50	500	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	Concentration required to inhibit neuronal nitric oxide synthase	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL	Inhibition of neuronal nitric oxide synthase (nNOS) in mice	B	6.3	pIC50	500	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of rat recombinant nNOS	B	5.34	pKi	4550	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of wild type rat nNOS by hemoglobin capture assay	B	5.22	pIC50	6000	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibition of recombinant rat nNOS expressed in Escherichia coli by hemoglobin capture assay	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL	Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL	Inhibition of rat nNOS L337H mutant by hemoglobin capture assay	B	5.4	pIC50	4000	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibition of rat brain nNOS expressed in baculovirus infected sf9 cells measured after 72 hrs	B	5.85	pIC50	1400	nM	IC50	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay	B	6.22	pIC50	600	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons)	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
GtoPdb	-	-	7.52	pKi	30	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme.	B	7.52	pKi	30	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS)	B	6.3	pIC50	500	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against recombinant human Endothelial nitric oxide synthase	B	6.49	pIC50	320	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of endothelial nitric oxide synthase.	B	6.49	pIC50	320	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Inhibition of bovine recombinant eNOS	B	6.12	pKi	750	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of bovine recombinant endothelial NOS by hemoglobin capture assay	B	6.14	pKi	720	nM	Ki	J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL	Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells	B	5.92	pIC50	1200	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
GtoPdb	-	-	6.17	pKi	670	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme.	B	6.17	pKi	670	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS)	B	5.12	pIC50	7600	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against human Inducible nitric oxide synthase	B	5.26	pIC50	5500	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of inducible nitric oxide synthase.	B	5.26	pIC50	5500	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Inhibition of iNOS in mouse RAW264.7 cells assessed as inhibition constant in presence of NADPH	B	5.36	pKi	4400	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of mouse recombinant inducible NOS by hemoglobin capture assay	B	5.37	pKi	4280	nM	Ki	J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL	Inhibition of mouse recombinant iNOS	B	6.24	pKi	570	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of inducible nitric oxide synthase (iNOS) in mice	B	4.1	pIC50	80000	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
ChEMBL	Inhibition of iNOS in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction incubated for 24 hrs by Griess assay	B	4.56	pIC50	27550	nM	IC50	Bioorg Med Chem (2020) 28: 115733-115733 [PMID:33065432]
ChEMBL	Inhibition of inducible NOS mediated nitric oxide production in LPS-activated Kunming mouse macrophages	B	4.83	pIC50	14740	nM	IC50	Bioorg Med Chem (2008) 16: 578-585 [PMID:17937988]
ChEMBL	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	B	4.85	pIC50	14000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Tested for inhibition of mouse inducible nitric oxide synthase	B	6.3	pIC50	500	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]
Inducible NOS/Nitric oxide synthase, inducible in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
ChEMBL	Tested for inhibition of rat brain inducible nitric oxide synthase	B	4.7	pIC50	20000	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]