L-NNA [Ligand Id: 8759] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL227744 (L-Nitroarginine, L-Nw-Nitroarginine, L-N-Nitroarginine)
  • Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
  • Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
  • Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
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  • Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
  • Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
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  • Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
  • Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
  • Inducible NOS/Nitric oxide synthase, inducible in Rat [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL Inhibition of nNOS assessed as conversion of L-[3H]arginine to L-[3H]citrulline B 6.3 pKi 500 nM Ki Bioorg. Med. Chem. (2008) 16: 6193-6206 [PMID:18477512]
GtoPdb - - 7.82 pKi 15 nM Ki J. Med. Chem. (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme. B 7.82 pKi 15 nM Ki J. Med. Chem. (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of human nNOS by hemoglobin capture assay B 5.36 pIC50 4400 nM IC50 J. Med. Chem. (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase B 6.3 pIC50 500 nM IC50 J. Med. Chem. (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS B 7.1 pIC50 80 nM IC50 Bioorg. Med. Chem. Lett. (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL Concentration required to inhibit neuronal nitric oxide synthase B 7.1 pIC50 80 nM IC50 Bioorg. Med. Chem. Lett. (2002) 12: 2561-2564 [PMID:12182860]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL Inhibition of neuronal nitric oxide synthase (nNOS) in mice B 6.3 pIC50 500 nM IC50 Bioorg. Med. Chem. Lett. (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL Inhibition of rat recombinant nNOS B 5.34 pKi 4550 nM Ki J. Med. Chem. (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of wild type rat nNOS by hemoglobin capture assay B 5.22 pIC50 6000 nM IC50 J. Med. Chem. (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibition of recombinant rat nNOS expressed in Escherichia coli by hemoglobin capture assay B 5.3 pIC50 5000 nM IC50 Bioorg. Med. Chem. (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay B 5.3 pIC50 5000 nM IC50 Bioorg. Med. Chem. (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL Inhibition of rat nNOS L337H mutant by hemoglobin capture assay B 5.4 pIC50 4000 nM IC50 J. Med. Chem. (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay B 6.22 pIC50 600 nM IC50 J. Med. Chem. (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons) B 6.22 pIC50 600 nM IC50 Bioorg. Med. Chem. Lett. (1996) 6: 999-1002
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
GtoPdb - - 7.52 pKi 30 nM Ki J. Med. Chem. (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme. B 7.52 pKi 30 nM Ki J. Med. Chem. (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS) B 6.3 pIC50 500 nM IC50 J. Med. Chem. (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against recombinant human Endothelial nitric oxide synthase B 6.49 pIC50 320 nM IC50 Bioorg. Med. Chem. Lett. (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL In vitro inhibition of endothelial nitric oxide synthase. B 6.49 pIC50 320 nM IC50 Bioorg. Med. Chem. Lett. (2002) 12: 2561-2564 [PMID:12182860]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL Inhibition of bovine recombinant eNOS B 6.12 pKi 750 nM Ki J. Med. Chem. (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of bovine recombinant endothelial NOS by hemoglobin capture assay B 6.14 pKi 720 nM Ki J. Med. Chem. (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells B 5.92 pIC50 1200 nM IC50 J. Med. Chem. (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
GtoPdb - - 6.17 pKi 670 nM Ki J. Med. Chem. (1998) 41: 2858-71 [PMID:9667974]
ChEMBL Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme. B 6.17 pKi 670 nM Ki J. Med. Chem. (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS) B 5.12 pIC50 7600 nM IC50 J. Med. Chem. (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL Inhibitory concentration against human Inducible nitric oxide synthase B 5.26 pIC50 5500 nM IC50 Bioorg. Med. Chem. Lett. (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL In vitro inhibition of inducible nitric oxide synthase. B 5.26 pIC50 5500 nM IC50 Bioorg. Med. Chem. Lett. (2002) 12: 2561-2564 [PMID:12182860]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL Inhibition of mouse recombinant inducible NOS by hemoglobin capture assay B 5.37 pKi 4280 nM Ki J. Med. Chem. (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL Inhibition of mouse recombinant iNOS B 6.24 pKi 570 nM Ki J. Med. Chem. (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL Inhibition of inducible nitric oxide synthase (iNOS) in mice B 4.1 pIC50 80000 nM IC50 Bioorg. Med. Chem. Lett. (2004) 14: 313-316 [PMID:14698148]
ChEMBL Inhibition of inducible NOS mediated nitric oxide production in LPS-activated Kunming mouse macrophages B 4.83 pIC50 14740 nM IC50 Bioorg. Med. Chem. (2008) 16: 578-585 [PMID:17937988]
ChEMBL Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells B 4.85 pIC50 14000 nM IC50 J. Med. Chem. (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase B 5 pIC50 10000 nM IC50 Bioorg. Med. Chem. Lett. (1996) 6: 999-1002
ChEMBL Tested for inhibition of mouse inducible nitric oxide synthase B 6.3 pIC50 500 nM IC50 J. Med. Chem. (1994) 37: 3886-3888 [PMID:7525961]
Inducible NOS/Nitric oxide synthase, inducible in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
ChEMBL Tested for inhibition of rat brain inducible nitric oxide synthase B 4.7 pIC50 20000 nM IC50 J. Med. Chem. (1994) 37: 3886-3888 [PMID:7525961]

ChEMBL data shown on this page come from version 27:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]