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ChEMBL ligand: CHEMBL2012582 |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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bromodomain containing 4/Bromodomain-containing protein 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885] | ||||||||
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRD4 expressed in Escherichia coli BL21 (DE3) cells by ITC analysis | B | 6.71 | pKd | 194 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRD4 expressed in Escherichia coli BL21 (DE3) cells by MST assay | B | 7.16 | pKd | 69 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRD4 expressed in Escherichia coli BL21 (DE3) cells by qPCR assay | B | 7.18 | pKd | 66 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
bromodomain testis associated/Bromodomain testis-specific protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795185] [GtoPdb: 2729] [UniProtKB: Q58F21] | ||||||||
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRDT expressed in Escherichia coli BL21 (DE3) cells by ITC analysis | B | 5.77 | pKd | 1700 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRDT expressed in Escherichia coli BL21 (DE3) cells by MST assay | B | 6.15 | pKd | 700 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
ChEMBL | Binding affinity to recombinant human N-terminal hexaHis-tagged BRDT expressed in Escherichia coli BL21 (DE3) cells by qPCR assay | B | 6.41 | pKd | 390 | nM | Kd | J Med Chem (2021) 64: 15772-15786 [PMID:34710325] |
leucine rich repeat kinase 2/Leucine-rich repeat serine/threonine-protein kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075104] [GtoPdb: 2059] [UniProtKB: Q5S007] | ||||||||
GtoPdb | Biochemical assay dissociation constant. | - | 8.22 | pKi | 6 | nM | Ki | Bioorg Med Chem Lett (2013) 23: 3690-6 [PMID:23721803] |
ChEMBL | Inhibition of GST-tagged LRRK2 (1326 to 2527 amino acids) G2019S mutant (unknown origin) | B | 8.22 | pKi | 6 | nM | Ki | Bioorg Med Chem Lett (2013) 23: 3690-3696 [PMID:23721803] |
ChEMBL | Inhibition of LRRK2 G2019S and A2016T mutant expressed in HEK293 cells using nictide and ATP as substrate | B | 5.51 | pIC50 | 3080 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 1864-1869 [PMID:22335897] |
ChEMBL | Inhibition of LRRK2 A2016T mutant expressed in HEK293 cells using nictide and ATP as substrate | B | 5.61 | pIC50 | 2450 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 1864-1869 [PMID:22335897] |
ChEMBL | Inhibition of recombinant catalytic human GST-tagged LLRK2 (970 to 2527 residues) expressed in baculovirus expression system using LRRKtide as substrate after 1 hr by alexaFluor-ADP-based FRET assay | B | 7.7 | pIC50 | 20 | nM | IC50 | Eur J Med Chem (2017) 138: 328-342 [PMID:28688273] |
ChEMBL | Inhibition of recombinant catalytic human GST-tagged LLRK2 G2019S mutant (970 to 2527 residues) expressed in baculovirus expression system using LRRKtide as substrate after 1 hr by alexaFluor-ADP-based FRET assay | B | 7.7 | pIC50 | 20 | nM | IC50 | Eur J Med Chem (2017) 138: 328-342 [PMID:28688273] |
ChEMBL | Inhibition of wild-type GST-tagged LRRK2 (1326 to 2527 aa)(unknown origin) stably expressed in HEK293 cell lysate using [gamma-32P] after 15 mins by Cerenkov counting method | B | 7.89 | pIC50 | 13 | nM | IC50 | Eur J Med Chem (2015) 95: 29-34 [PMID:25791676] |
ChEMBL | Inhibition of wild type GST-tagged LRRK2 ((1326 to 2517 amino acids) (unknown origin) | B | 7.89 | pIC50 | 13 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 3690-3696 [PMID:23721803] |
ChEMBL | Inhibition of wild-type LRRK2 expressed in HEK293 cells using nictide and [gamma32]ATP as substrate | B | 7.89 | pIC50 | 13 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 1864-1869 [PMID:22335897] |
GtoPdb | With 0.1 mM ATP in the assay. | - | 7.89 | pIC50 | 13 | nM | IC50 | Nat Chem Biol (2011) 7: 203-5 [PMID:21378983] |
ChEMBL | Inhibition of LRRK2 G2019S mutant (1326 to 252 aa) (unknown origin) stably expressed in HEK293 cell lysate using [gamma-32P] after 15 mins by Cerenkov counting method | B | 8.1 | pIC50 | 7.9 | nM | IC50 | Eur J Med Chem (2015) 95: 29-34 [PMID:25791676] |
ChEMBL | Inhibition of GST-LRRK2 (1326 to 2527 residues) G2019S mutant (unknown origin) expressed in HEK293 cells incubated for 15 mins by cerenkov counting method | B | 8.22 | pIC50 | 6 | nM | IC50 | J Med Chem (2020) 63: 11330-11361 [PMID:32352776] |
ChEMBL | Inhibition of LRRK2 G2019S mutant expressed in HEK293 cells using nictide and ATP as substrate | B | 8.22 | pIC50 | 6 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 1864-1869 [PMID:22335897] |
ChEMBL | Inhibition of GST-tagged LRRK2 (1326 to 2527 amino acids) G2019S mutant (unknown origin) | B | 8.22 | pIC50 | 6 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 3690-3696 [PMID:23721803] |
ChEMBL | Inhibition of LRRK2 (unknown origin) | B | 8.22 | pIC50 | 6 | nM | IC50 | J Med Chem (2015) 58: 6733-6746 [PMID:25915084] |
ChEMBL | Inhibition of recombinant LRRK2 (unknown origin) using gamma-32P-ATP assessed as LRRKtide substrate phosphorylation level by autoradiography | B | 8.46 | pIC50 | 3.5 | nM | IC50 | Bioorg Med Chem Lett (2014) 24: 4630-4637 [PMID:25219901] |
ChEMBL data shown on this page come from version 32:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]