ChEMBL ligand: CHEMBL2018302 (Tubastatin A)
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] histone deacetylase 1/Histone deacetylase 1 in Mouse [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 10/Histone deacetylase 10 in Human [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 11/Histone deacetylase 11 in Human [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 2/Histone deacetylase 2 in Human [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3 in Human [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 4/Histone deacetylase 4 in Human [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 5/Histone deacetylase 5 in Human [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] histone deacetylase 6/Histone deacetylase 6 in Mouse [ChEMBL: CHEMBL2878] [GtoPdb: 2618] [UniProtKB: Q9Z2V5] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 7/Histone deacetylase 7 in Human [ChEMBL: CHEMBL2716] [GtoPdb: 2661] [UniProtKB: Q8WUI4] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase 8 in Schistosoma mansoni [ChEMBL: CHEMBL3797017] [UniProtKB: A5H660] histone deacetylase 8/Histone deacetylase 8 in Human [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 9/Histone deacetylase 9 in Human [ChEMBL: CHEMBL4145] [GtoPdb: 2620] [UniProtKB: Q9UKV0] There should be some charts here, you may need to enable JavaScript!
Polyamine deacetylase HDAC10 in Danio rerio [ChEMBL: CHEMBL4802002] [UniProtKB: F1QCV2] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Inhibition of human HDAC1	B	5.42	pKi	3823.3	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Binding affinity to recombinant HDAC1 (unknown origin) assessed as inhibition constant using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	5.89	pKi	1280	nM	Ki	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant human HDAC1 using RHKKAc fluorogenic peptide substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2019) 62: 1138-1166 [PMID:30645113]
ChEMBL	Inhibition of human HDAC1 using p53 fluorogenic peptide (379 to 382 residues) RHKKAc as substrate by fluorescence-based assay	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2018) 157: 447-461 [PMID:30103193]
ChEMBL	Inhibition of human recombinant HDAC1 protein using RHKKAc from p53 as substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human recombinant HDAC1 using RHKKAc as substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2013) 56: 7201-7211 [PMID:23964961]
ChEMBL	Inhibition of full length GST-tagged human HDAC1 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	4.79	pIC50	16400	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2015) 96: 340-359 [PMID:25899338]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKKAc fluorogenic peptide as substrate by fluorescence assay	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of HADC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of human recombinant HDAC1 using RHKKAc peptide as substrate incubated for 5 to mins prior to substrate addition measured after 2 hrs	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2012) 55: 639-651 [PMID:22165909]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic peptide RHKKAc-AMC as substrate by fluorescence-based assay	B	4.79	pIC50	16400	nM	IC50	Bioorg Med Chem Lett (2023) 81: 129148-129148 [PMID:36690041]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2012) 55: 9891-9899 [PMID:23009203]
ChEMBL	Inhibition of human recombinant HDAC1 using RHKKAc as substrate	B	4.79	pIC50	16400	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2017) 135: 174-195 [PMID:28453994]
ChEMBL	Inhibition of human recombinant full-length HDAC1 (1 to 482 residues) expressed in baculovirus using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	4.79	pIC50	16400	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of recombinant human HDAC1 using Fluor de Lys-SIRT1 as substrate incubated for 15 mins by fluorescence assay	B	4.84	pIC50	14350	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
GtoPdb	-	-	4.86	pIC50	13800	nM	IC50	US8748451. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at room temperature in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	4.86	pIC50	13800	nM	IC50	US-8748451-B2. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf21 insect cells by using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	5	pIC50	>10000	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-His-tagged HDAC1 expressed in Sf21 cells using RHKK(Ac) as substrate after 60 mins by fluorimetric method	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2017) 127: 115-127 [PMID:28038324]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	5.09	pIC50	8100	nM	IC50	J Med Chem (2020) 63: 10246-10262 [PMID:32815366]
ChEMBL	Inhibition of human HDAC1	B	5.09	pIC50	8100	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of human full length recombinant HDAC1 using p53 (379 to 382 residues) (RHKK(Ac)AMC) as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence method	B	5.1	pIC50	8000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and further incubated for incubated for 1 hrs by fluorescence microtiter plate reader assay	B	5.12	pIC50	7582.5	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of recombinant human HDAC1 using Z-(Ac)-Lys-AMC as substrate incubated for 40 mins by fluorescence based assay	B	5.15	pIC50	7048	nM	IC50	Eur J Med Chem (2021) 218: 113392-113392 [PMID:33831778]
ChEMBL	Inhibition of HDAC1 (unknown origin) measured after 30 mins by fluorescence microplate reader assay	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of recombinant human HDAC1 using RHKKAc peptide as substrate	B	5.49	pIC50	3259	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2021) 64: 26-41 [PMID:33346659]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	5.52	pIC50	>3000	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate measured after 30 mins	B	5.53	pIC50	2980	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate measured after 30 mins	B	5.53	pIC50	2977	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of recombinant human KDAC1 using acetylated p53 (379 to 382 residues) as substrate by fluorescence assay	B	5.54	pIC50	2870	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of HDAC1 in human HeLa-S3 cell lysates preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 40 mins by fluorimetric method	B	5.57	pIC50	2700	nM	IC50	Eur J Med Chem (2018) 143: 1790-1806 [PMID:29150330]
ChEMBL	Inhibition of full length his6/FLAG-tagged human recombinant HDAC1(1 to 482 residues) expressed in Sf9 insect cells	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2022) 65: 14764-14791 [PMID:36306372]
ChEMBL	Inhibition of HDAC1 in human HeLaS3 cells preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 45 mins by ELISA	B	5.57	pIC50	2700	nM	IC50	ACS Med Chem Lett (2017) 8: 281-286 [PMID:28337317]
ChEMBL	Inhibition of human recombinant HDAC1 using ZMAL (Z-Lys(Ac)-AMC) fluorogenic substrate incubated for 90 mins by fluorescence based assay	B	5.6	pIC50	2490	nM	IC50	J Med Chem (2020) 63: 10339-10351 [PMID:32803970]
ChEMBL	Inhibition of recombinant full length C-terminal FLAG/His-tagged human HDAC1 expressed in baculovirus infected Sf9 cells using Z-Lys(Ac)-AMC as substrate incubated for 90 mins followed by trypsin addition and measured after 30 mins by fluorescence based assay	B	5.6	pIC50	2490	nM	IC50	Medchemcomm (2019) 10: 1109-1115 [PMID:31391882]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis	B	5.6	pIC50	2490	nM	IC50	J Med Chem (2022) 65: 15457-15472 [PMID:36351184]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	5.65	pIC50	2240	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of human recombinant GST-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells using MOCPAC as substrate after 4 hrs by UHPLC-ESI-MS/MS analysis	B	5.7	pIC50	>2000	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of full length C-terminal His/FLAG-tagged human HDAC1 expressed in baculovirus expression system using ZMAL as substrate incubated for 90 mins by fluorescence assay	B	5.72	pIC50	1910	nM	IC50	ACS Med Chem Lett (2020) 11: 2268-2276 [PMID:33214839]
ChEMBL	Inhibition of recombinant C-terminal His/FLAG-tagged human HDAC1 expressed in baculovirus expression system using fluorogenic ZMAL as substrate incubated for 90 mins by fluorescence assay	B	5.72	pIC50	1910	nM	IC50	J Med Chem (2019) 62: 1138-1166 [PMID:30645113]
ChEMBL	Inhibition of recombinant human HDAC1 using ZMAL (Z-(Ac)Lys-AMC as substrate incubated for 20 mins and measured by homogenous fluorescence assay	B	5.72	pIC50	1910	nM	IC50	Eur J Med Chem (2022) 234: 114272-114272 [PMID:35306288]
ChEMBL	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	5.85	pIC50	1400	nM	IC50	US-9249087-B2. HDAC inhibitors and therapeutic methods using the same (2016)
ChEMBL	Inhibition of HDAC1 in human SHSY5Y cells using MOCPAC as substrate after 8 hrs by UHPLC-ESI-MS/MS analysis	B	5.95	pIC50	1109.7	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of human HDAC1 pre-incubated for 5 mins before substrate addition and measured after 30 mins by fluorescence based assay	B	6.12	pIC50	752	nM	IC50	J Med Chem (2021) 64: 1116-1126 [PMID:33356256]
ChEMBL	Inhibition of HDAC1 (unknown origin) measured by fluorescence based assay	B	6.41	pIC50	392	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	Inhibition of HDAC1 (unknown origin) assessed as fluorescence intensity measured after 60 mins incubation at room temperature by trypsin-free microfluidic lab-on-a-chip assay	B	6.84	pIC50	144	nM	IC50	J Med Chem (2013) 56: 1772-1776 [PMID:23368884]
histone deacetylase 1/Histone deacetylase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106]
ChEMBL	Inhibition of HDAC1 in mouse N2A cells	B	5.09	pIC50	8100	nM	IC50	ACS Med Chem Lett (2020) 11: 706-712 [PMID:32435374]
histone deacetylase 10/Histone deacetylase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of human recombinant HDAC10 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human recombinant HDAC10 using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 7201-7211 [PMID:23964961]
ChEMBL	Inhibition of N-terminal GST-tagged human HDAC10 (1 to 481 residues) using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at room temperature in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	4.52	pIC50	>30000	nM	IC50	US-8748451-B2. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Inhibition of HDAC10 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC10 (unknown origin) using RHKKAc fluorogenic peptide as substrate by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of human recombinant HDAC10 protein using RHKKAc from p53 as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 135: 174-195 [PMID:28453994]
ChEMBL	Inhibition of human full length recombinant HDAC10 using p53 (379 to 382 residues) (RHKK(Ac)AMC) as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence method	B	4.6	pIC50	25000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	5.43	pIC50	3710	nM	IC50	US-9249087-B2. HDAC inhibitors and therapeutic methods using the same (2016)
ChEMBL	Inhibition of dye-labeled tracer binding to HDAC10 (unknown origin) transfected in human HeLa cells measured after 2 hrs by nano-luciferase reporter gene-based BRET assay	B	7.9	pIC50	12.59	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
ChEMBL	Inhibition of tubastatin-Alexa647-tracer binding to recombinant GST-tagged HDAC10 (unknown origin) measured after 1 hr by TR-FRET assay	B	7.9	pIC50	12.59	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
histone deacetylase 11/Histone deacetylase 11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of human recombinant HDAC11 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human recombinant HDAC11 using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 7201-7211 [PMID:23964961]
ChEMBL	Inhibition of full length N-terminal GST-tagged human HDAC11 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at room temperature in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	4.52	pIC50	>30000	nM	IC50	US-8748451-B2. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 135: 174-195 [PMID:28453994]
ChEMBL	Inhibition of HDAC11 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC11 (unknown origin) using RHKKAc fluorogenic peptide as substrate by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of human recombinant HDAC11 protein using RHKKAc from p53 as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of human full length recombinant HDAC11 using p53 (379 to 382 residues) (RHKK(Ac)AMC) as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence method	B	4.8	pIC50	16000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of human recombinant HDAC11 using fluorogenic substrate measured after 30 mins	B	5.06	pIC50	8780	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of recombinant human HDAC11 measured after 30 mins by fluorescence microplate reader assay	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of HDAC11 (unknown origin) measured by fluorescence based assay	B	5.3	pIC50	>5000	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	5.42	pIC50	3790	nM	IC50	US-9249087-B2. HDAC inhibitors and therapeutic methods using the same (2016)
histone deacetylase 2/Histone deacetylase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769]
ChEMBL	Inhibition of C-terminal GST-tagged recombinant human HDAC2 (1 to 488 residues) expressed in Baculovirus infected insect cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and further incubation for 90 mins measured after 15 mins by microplate reader based fluorescence assay	B	5.24	pKi	5770	nM	Ki	Bioorg Med Chem (2019) 27: 115036-115036 [PMID:31431326]
ChEMBL	Inhibition of human recombinant HDAC2 using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of human recombinant HDAC2 protein using RHKKAc from p53 as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of human recombinant HDAC2 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human recombinant HDAC2 using RHKKAc as substrate	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2013) 56: 7201-7211 [PMID:23964961]
ChEMBL	Inhibition of full length C-terminal 6x-His tagged human HDAC2 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at room temperature in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	4.52	pIC50	>30000	nM	IC50	US-8748451-B2. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 135: 174-195 [PMID:28453994]
ChEMBL	Inhibition of human recombinant full-length C-terminal GST-tagged HDAC2 expressed in baculovirus infected Sf9 cells using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of HADC2 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of human HDAC2 using p53 fluorogenic peptide (379 to 382 residues) RHKKAc as substrate by fluorescence-based assay	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKKAc fluorogenic peptide as substrate by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of human full length recombinant HDAC2 using p53 (379 to 382 residues) (RHKK(Ac)AMC) as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence method	B	4.72	pIC50	19000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of full length human recombinant C-terminal GST-tagged HDAC2 expressed in insect cells using RHKK(Ac) as substrate after 60 mins by fluorimetric method	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2017) 127: 115-127 [PMID:28038324]
ChEMBL	Inhibition of recombinant human C-terminal GST-tagged HDAC2 (1 to 488 residues) expressed in insect cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	5	pIC50	>10000	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and further incubated for incubated for 1 hrs by fluorescence microtiter plate reader assay	B	5.06	pIC50	8674.5	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	5.2	pIC50	6270	nM	IC50	US-9249087-B2. HDAC inhibitors and therapeutic methods using the same (2016)
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of HDAC2 (unknown origin) measured after 30 mins by fluorescence microplate reader assay	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of HDAC2 in human HeLa-S3 cell lysates preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 40 mins by fluorimetric method	B	5.41	pIC50	3900	nM	IC50	Eur J Med Chem (2018) 143: 1790-1806 [PMID:29150330]
ChEMBL	Inhibition of HDAC2 in human HeLaS3 cells preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 45 mins by ELISA	B	5.41	pIC50	3900	nM	IC50	ACS Med Chem Lett (2017) 8: 281-286 [PMID:28337317]
ChEMBL	Inhibition of full length C-terminal his6-tagged human recombinant HDAC2 (1 to 488 residues)	B	5.41	pIC50	3900	nM	IC50	J Med Chem (2022) 65: 14764-14791 [PMID:36306372]
ChEMBL	Inhibition of recombinant human HDAC2 using RHKKAc peptide as substrate	B	5.45	pIC50	3575	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	5.52	pIC50	>3000	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of human recombinant HDAC2 using fluorogenic substrate measured after 30 mins	B	5.53	pIC50	2920	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of HDAC2 (unknown origin) assessed as fluorescence intensity measured after 60 mins incubation at room temperature by trypsin-free microfluidic lab-on-a-chip assay	B	6.44	pIC50	360	nM	IC50	J Med Chem (2013) 56: 1772-1776 [PMID:23368884]
histone deacetylase 3/Histone deacetylase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379]
ChEMBL	Inhibition of HDAC3 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of full length C-terminal 6x-His tagged human HDAC3 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at room temperature in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	B	4.52	pIC50	>30000	nM	IC50	US-8748451-B2. HDAC inhibitors and therapeutic methods of using same (2014)
ChEMBL	Inhibition of HDAC3 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 135: 174-195 [PMID:28453994]
ChEMBL	Inhibition of human recombinant full-length C-terminal GST-tagged HDAC3 expressed in baculovirus infected Sf9 cells using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of HADC3 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of HDAC3 (unknown origin)	B	4.52	pIC50	>30000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC3 (unknown origin) using RHKKAc fluorogenic peptide as substrate by fluorescence assay	B	4.52	pIC50