ChEMBL ligand: CHEMBL13486 (3',4',7-Trihydroxyisoflavone, 7,3',4'-Trihydroxyisoflavone)
Macrophage migration inhibitory factor in Human [ChEMBL: CHEMBL2085] [UniProtKB: P14174] There should be some charts here, you may need to enable JavaScript!
tyrosinase/Tyrosinase in Mouse [ChEMBL: CHEMBL5346] [GtoPdb: 2643] [UniProtKB: P11344] There should be some charts here, you may need to enable JavaScript!
xanthine dehydrogenase/Xanthine dehydrogenase in Human [ChEMBL: CHEMBL1929] [GtoPdb: 2646] [UniProtKB: P47989] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Macrophage migration inhibitory factor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2085] [UniProtKB: P14174]
ChEMBL	Inhibition of recombinant human MIF tautomerase activity expressed in Escherichia coli using 4-hydroxyphenyl pyruvic acid as substrate assessed as borate-enol complex formation incubated for 30 mins followed substrate addition by microplate reader	B	4.82	pKi	15000	nM	Ki	Bioorg Med Chem Lett (2016) 26: 2764-2767 [PMID:27156768]
ChEMBL	Inhibitory activity against tautomerase macrophage migration inhibitory factor (MIF)	B	7.42	pKi	38	nM	Ki	J Med Chem (2001) 44: 540-547 [PMID:11170644]
ChEMBL	Inhibition of human recombinant MIF tautomerase	B	7.42	pKi	38	nM	Ki	J Med Chem (2009) 52: 416-424 [PMID:19090668]
ChEMBL	Inhibition of human MIF tautomerase activity expressed in Escherichia coli BL21 (DE3) using 4-hydroxyphenyl pyruvic acid as substrate	B	7.42	pIC50	38	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2764-2767 [PMID:27156768]
ChEMBL	Inhibition of recombinant human His-tagged MIF tautomerization expressed in Escherichia coli BL21 (DE3) using D-dopachrome as substrate after 10 mins by spectrophotometric analysis	B	7.42	pIC50	38	nM	IC50	J Med Chem (2018) 61: 8104-8119 [PMID:29812929]
tyrosinase/Tyrosinase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5346] [GtoPdb: 2643] [UniProtKB: P11344]
ChEMBL	Inhibition of tyrosinase in mouse Melan-a cells by ELISA	B	5.28	pIC50	5230	nM	IC50	Bioorg Med Chem Lett (2010) 20: 1162-1164 [PMID:20022495]
xanthine dehydrogenase/Xanthine dehydrogenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1929] [GtoPdb: 2646] [UniProtKB: P47989]
ChEMBL	Inhibition of xanthine oxidase assessed as decrease of superoxide generation	B	4.77	pIC50	16800	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5006-5009 [PMID:18722771]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]