Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	506	13q14.3	NEK3	NIMA related kinase 3
Mouse	-	511	8 11.07 cM	Nek3	NIMA (never in mitosis gene a)-related expressed kinase 3
Rat	-	468	16q12.5	Nek3	NIMA-related kinase 3

Previous and Unofficial Names

HSPK36 | phosphorylase B kinase kinase | NIMA-related kinase 3

Database Links
Alphafold	P51956 (Hs), Q9R0A5 (Mm)
BRENDA	2.7.11.1
ChEMBL Target	CHEMBL5679 (Hs)
Ensembl Gene	ENSG00000136098 (Hs), ENSMUSG00000031478 (Mm), ENSRNOG00000012757 (Rn)
Entrez Gene	4752 (Hs), 23954 (Mm), 306576 (Rn)
Human Protein Atlas	ENSG00000136098 (Hs)
KEGG Enzyme	2.7.11.1
KEGG Gene	hsa:4752 (Hs), mmu:23954 (Mm), rno:306576 (Rn)
OMIM	604044 (Hs)
Pharos	P51956 (Hs)
RefSeq Nucleotide	NM_002498 (Hs), NM_001162947 (Mm), XM_224971 (Rn)
RefSeq Protein	NP_002489 (Hs), NP_001156419 (Mm), XP_224971 (Rn)
UniProtKB	P51956 (Hs), Q9R0A5 (Mm)
Wikipedia	NEK3 (Hs)

Enzyme Reaction
EC Number: 2.7.11.1

Download all structure-activity data for this target as a CSV file

Inhibitors
Key to terms and symbols	View all chemical structures	Click column headers to sort
Ligand Sp. Action Value Parameter Reference compound 25 [PMID: 17935989] Hs Inhibition <5.8 pKi 4 ⤷ pKi <5.8 (Ki >1.667x10-6 M) [4]

DiscoveRx KINOMEscan® screen
A screen of 72 inhibitors against 456 human kinases. Quantitative data were derived using DiscoveRx KINOMEscan® platform. http://www.discoverx.com/services/drug-discovery-development-services/kinase-profiling/kinomescan Reference: 2,5
Key to terms and symbols		Click column headers to sort
Target used in screen: NEK3
Ligand Sp. Type Action Value Parameter tamatinib Hs Inhibitor Inhibition 6.8 pKd fedratinib Hs Inhibitor Inhibition 6.2 pKd BI-2536 Hs Inhibitor Inhibition 6.1 pKd KW-2449 Hs Inhibitor Inhibition 6.0 pKd ruxolitinib Hs Inhibitor Inhibition 6.0 pKd neratinib Hs Inhibitor Inhibition 5.6 pKd SB203580 Hs Inhibitor Inhibition <5.5 pKd ruboxistaurin Hs Inhibitor Inhibition <5.5 pKd erlotinib Hs Inhibitor Inhibition <5.5 pKd linifanib Hs Inhibitor Inhibition <5.5 pKd
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EMD Millipore KinaseProfilerTM screen/Reaction Biology Kinase HotspotSM screen
A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfilerTM service. A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase HotspotSM platform. http://www.millipore.com/techpublications/tech1/pf3036 http://www.reactionbiology.com/webapps/main/pages/kinase.aspx Reference: 1,3
Key to terms and symbols		Click column headers to sort
Target used in screen: NEK3/NEK3
Ligand Sp. Type Action % Activity remaining at 0.5µM % Activity remaining at 1µM % Activity remaining at 10µM Cdk1/2 inhibitor III Hs Inhibitor Inhibition 19.6 6.0 3.0 Tpl2 kinase inhibitor Hs Inhibitor Inhibition 56.0 37.0 46.0 TWS119 Hs Inhibitor Inhibition 57.5 42.0 23.0 Syk inhibitor Hs Inhibitor Inhibition 66.2 72.0 19.0 Cdk2 inhibitor IV Hs Inhibitor Inhibition 69.4 47.0 18.0 JAK inhibitor I Hs Inhibitor Inhibition 74.4 92.0 46.0 EGFR/ErbB-2/ErbB-4 inhibitor Hs Inhibitor Inhibition 76.2 107.0 84.0 GSK-3beta inhibitor VIII Hs Inhibitor Inhibition 78.6 74.0 96.0 PKR inhibitor Hs Inhibitor Inhibition 80.2 102.0 32.0 Cdc2-like kinase inhibitor Hs Inhibitor Inhibition 82.5 81.0 91.0
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