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TRPC3

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Immunopharmacology Ligand target has curated data in GtoImmuPdb

Target id: 488

Nomenclature: TRPC3

Family: Transient Receptor Potential channels (TRP)

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 836 4q27 TRPC3 transient receptor potential cation channel subfamily C member 3 38
Mouse 6 1 836 3 17.93 cM Trpc3 transient receptor potential cation channel, subfamily C, member 3 23
Rat 6 1 836 2q25 Trpc3 transient receptor potential cation channel, subfamily C, member 3 24
Previous and Unofficial Names Click here for help
TRP3 [38] | short transient receptor potential channel 3 | transient receptor protein 3 | trp-related protein 3 | transient receptor potential cation channel
Database Links Click here for help
Alphafold
CATH/Gene3D
ChEMBL Target
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
Pharos
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Associated Proteins Click here for help
Heteromeric Pore-forming Subunits
Name References
TRPC6 5
TRPC7 5
TRPC1 36-37
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
VAMP2 32
MxA 19
Sodium/calcium exchanger 1 29
syntaxin 3
FKBP prolyl isomerase 1A 32
C-terminal tail-truncated TRPP2 22
IP3R1 1
GluD2 14
Functional Characteristics Click here for help
γ = 66 pS; conducts mono and di-valent cations non-selectively (PCa/PNa = 1.6); monovalent cation current suppressed by extracellular Ca2+; dual (inward and outward) rectification
Ion Selectivity and Conductance Click here for help
Species:  Human
Rank order:  Ca2+ > Na+ [66.0 pS]
References:  39
Ion Selectivity and Conductance Comments
Non-selective cation channel.
Chemical activators (Human)
diacylglycerols

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Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
OAG Small molecule or natural product Click here for species-specific activity table Hs Agonist - - 1x10-4 -60.0 10
Conc range: 1x10-4 M [10]
Holding voltage: -60.0 mV
pyrazolopyrimidine 4n Small molecule or natural product Click here for species-specific activity table Hs Activation 7.7 pEC50 - - 26
pEC50 7.7 (EC50 1.9x10-8 M) [26]
GSK1702934A Small molecule or natural product Click here for species-specific activity table Hs Agonist 7.1 pEC50 - - 35
pEC50 7.1 (EC50 8x10-8 M) [35]
Description: Activation of hTRPC3-mediated currents in HEK293 cells in whole-cell patch-clamp experiments.
Channel Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
norgestimate Small molecule or natural product Approved drug Click here for species-specific activity table Hs - 5.5 pKi - - 21
pKi 5.5 (Ki 3x10-6 M) [21]
Description: Norgestimate-induced inhibition of hTRPC3-mediated Ca2+ entry in transfected CHO cells stimulated with the OAG diacylglycerol analog.
Gd3+ Click here for species-specific activity table Hs Antagonist 7.0 pEC50 - -60.0 7
pEC50 7.0 (EC50 1x10-7 M) [7]
Holding voltage: -60.0 mV
GSK2833503A Small molecule or natural product Click here for species-specific activity table Hs - 7.7 pIC50 - 80.0 31
pIC50 7.7 (IC50 2.1x10-8 M) [31]
Holding voltage: 80.0 mV
Description: Measuring whole-cell current by patch-clamp in HEK cells expressing hTRPC3 with cation flux activated by OAG.
GSK417651A Small molecule or natural product Click here for species-specific activity table Hs Antagonist 7.1 pIC50 - - 34
pIC50 7.1 (IC50 8x10-8 M) [34]
Description: Inhibition of recombinant hTRPC3-mediated, carbachol-stimulated Ca2+/Na+ influx in HEK293-MSRII cells assessed by FLIPR assay.
SAR7334 Small molecule or natural product Click here for species-specific activity table Ligand has a PDB structure Hs - 6.6 pIC50 - - 20
pIC50 6.6 (IC50 2.82x10-7 M) [20]
BTP2 Small molecule or natural product Click here for species-specific activity table Immunopharmacology Ligand Hs Antagonist 6.5 pIC50 - -80.0 9
pIC50 6.5 (IC50 3x10-7 M) [9]
Holding voltage: -80.0 mV
Pyr3 Small molecule or natural product Hs - 6.2 pIC50 - - 15
pIC50 6.2 (IC50 7x10-7 M) [15]
Pyr10 Small molecule or natural product Hs Antagonist 6.1 pIC50 - - 30
pIC50 6.1 (IC50 7.2x10-7 M) [30]
Description: Measuring blockade of TRPC3-mediated receptor operated Ca2+ entry in vitro.
SH045 Small molecule or natural product Click here for species-specific activity table Hs - 6.1 pIC50 - - 11
pIC50 6.1 (IC50 8.4x10-7 M) [11]
Description: Measuring inhibition of calcium flux in HEK293 cells expressing hTRPC3, in a FLIPR assay.
La3+ Click here for species-specific activity table Hs Antagonist 5.4 pIC50 - -60.0 7
pIC50 5.4 (IC50 4x10-6 M) [7]
Holding voltage: -60.0 mV
clemizole Small molecule or natural product Click here for species-specific activity table Ligand has a PDB structure Immunopharmacology Ligand Hs - 5.0 pIC50 - - 28
pIC50 5.0 (IC50 9.1x10-6 M) [28]
2-APB Small molecule or natural product Click here for species-specific activity table Ligand has a PDB structure Hs Antagonist 5.0 pIC50 - Physiological 18
pIC50 5.0 (IC50 1x10-5 M) [18]
Holding voltage: Physiological
SKF96365 Small molecule or natural product Click here for species-specific activity table Hs - - - - -
ACAA Small molecule or natural product Click here for species-specific activity table Ligand has a PDB structure Hs - - - - -
Ni2+ Click here for species-specific activity table Hs - - - - -
KB-R7943 Small molecule or natural product Click here for species-specific activity table Hs - - - - -
Immunopharmacology Comments
Expressed on B cells, T cells, neutrophils and monocytes/macrophages [25].
Cell Type Associations
Immuno Cell Type:  T cells
Comment:  TRPC channels (e.g. TRPC3, TRPC5, TRPC6) are involved in TCR signalling, proliferation and autoimmune suppression.
References:  25
Immuno Cell Type:  Granulocytes
Comment:  TRPC channels (e.g. TRPC3, TRPC6) are involved in chemotaxis, LTC4 secretion and PAF signalling
References:  25
Immuno Cell Type:  Macrophages & monocytes
Comment:  TRPC channels (e.g. TRPC3, TRPC6) are involved in macrophage activation.
References:  25
Tissue Distribution Click here for help
Pituitary gland > brain, heart, lung, dorsal root ganglia.
Species:  Human
Technique:  RT-PCR
References:  27
Brain, thymus, spleen, liver, kidney, ovary, dorsal root ganglion, skin, skeletal and smooth muscle, retina, heart, lung, testis, innear ear.
Species:  Mouse
Technique:  RT-PCR, northern blot
References:  6,12-13,33
Functional Assays Click here for help
Suppression of oxidant-induced membrane currents by expression of a dominant negative fragment of hTRPC3.
Species:  Human
Tissue:  Aortic endothelial cells.
Response measured:  The tBHP-induced membrane depolarization was reduced in N-terminal fragment of hTrp3-transfected endothelial cells.
References:  2
Activation of TrkB and PLC leads to a TRPC3-dependent cation influx in SNS neurones.
Species:  Rat
Tissue:  Brain.
Response measured:  BDNF-induced cation influx mediated by TRPC3 channel.
References:  16
TRPC3 contributes to the BDNF-induced chemo-attractive turning.
Species:  Rat
Tissue:  Cerebellum
Response measured: 
References:  17
Physiological Functions Click here for help
TRPC3 null mice exhibit an impaired walking behavior.
Species:  Mouse
Tissue:  Brain
References:  8
A point mutation in TRPC3 causes cerebellar ataxia in moonwalker mice.
Species:  Mouse
Tissue:  Brain
References:  4
Phenotypes, Alleles and Disease Models Click here for help Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Trpc3tm1.1Akon Trpc3tm1.1Akon/Trpc3tm1.1Akon
involves: 129S/SvEv * 129S4/SvJaeSor
MGI:109526  MP:0002910 abnormal excitatory postsynaptic currents PMID: 18701065 
Trpc3tm1.1Akon Trpc3tm1.1Akon/Trpc3tm1.1Akon
involves: 129S/SvEv * 129S4/SvJaeSor
MGI:109526  MP:0001406 abnormal gait PMID: 18701065 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0001406 abnormal gait PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0002272 abnormal nervous system electrophysiology PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0008572 abnormal Purkinje cell dendrite morphology PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0000877 abnormal Purkinje cell morphology PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0001265 decreased body size PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0000880 decreased Purkinje cell number PMID: 19351902 
Trpc3+|Trpc3Mwk Trpc3Mwk/Trpc3+
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0001525 impaired balance PMID: 19351902 
Trpc3tm1.1Akon Trpc3tm1.1Akon/Trpc3tm1.1Akon
involves: 129S/SvEv * 129S4/SvJaeSor
MGI:109526  MP:0001524 impaired limb coordination PMID: 18701065 
Trpc3Mwk Trpc3Mwk/Trpc3Mwk
involves: BALB/cAnN * C3H/HeH
MGI:109526  MP:0002083 premature death PMID: 19351902 

References

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1. Adebiyi A, Zhao G, Narayanan D, Thomas-Gatewood CM, Bannister JP, Jaggar JH. (2010) Isoform-selective physical coupling of TRPC3 channels to IP3 receptors in smooth muscle cells regulates arterial contractility. Circ Res, 106 (10): 1603-12. [PMID:20378853]

2. Balzer M, Lintschinger B, Groschner K. (1999) Evidence for a role of Trp proteins in the oxidative stress-induced membrane conductances of porcine aortic endothelial cells. Cardiovasc Res, 42 (2): 543-9. [PMID:10533589]

3. Bandyopadhyay BC, Swaim WD, Liu X, Redman RS, Patterson RL, Ambudkar IS. (2005) Apical localization of a functional TRPC3/TRPC6-Ca2+-signaling complex in polarized epithelial cells. Role in apical Ca2+ influx. J Biol Chem, 280 (13): 12908-16. [PMID:15623527]

4. Becker EB, Oliver PL, Glitsch MD, Banks GT, Achilli F, Hardy A, Nolan PM, Fisher EM, Davies KE. (2009) A point mutation in TRPC3 causes abnormal Purkinje cell development and cerebellar ataxia in moonwalker mice. Proc Natl Acad Sci USA, 106 (16): 6706-11. [PMID:19351902]

5. Dietrich A, Kalwa H, Rost BR, Gudermann T. (2005) The diacylgylcerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch, 451 (1): 72-80. [PMID:15971081]

6. Gilliam JC, Wensel TG. (2011) TRP channel gene expression in the mouse retina. Vision Res, 51 (23-24): 2440-52. [PMID:22037305]

7. Halaszovich CR, Zitt C, Jungling E, Luckhoff A. (2000) Inhibition of TRP3 channels by lanthanides. Block from the cytosolic side of the plasma membrane. J Biol Chem, 275 (48): 37423-8. [PMID:10970899]

8. Hartmann J, Dragicevic E, Adelsberger H, Henning HA, Sumser M, Abramowitz J, Blum R, Dietrich A, Freichel M, Flockerzi V et al.. (2008) TRPC3 channels are required for synaptic transmission and motor coordination. Neuron, 59 (3): 392-8. [PMID:18701065]

9. He LP, Hewavitharana T, Soboloff J, Spassova MA, Gill DL. (2005) A functional link between store-operated and TRPC channels revealed by the 3,5-bis(trifluoromethyl)pyrazole derivative, BTP2. J Biol Chem, 280 (12): 10997-1006. [PMID:15647288]

10. Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T, Schultz G. (1999) Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature, 397 (6716): 259-63. [PMID:9930701]

11. Häfner S, Burg F, Kannler M, Urban N, Mayer P, Dietrich A, Trauner D, Broichhagen J, Schaefer M. (2018) A (+)-Larixol Congener with High Affinity and Subtype Selectivity toward TRPC6. ChemMedChem, 13 (10): 1028-1035. [PMID:29522264]

12. Inada H, Iida T, Tominaga M. (2006) Different expression patterns of TRP genes in murine B and T lymphocytes. Biochem Biophys Res Commun, 350 (3): 762-7. [PMID:17027915]

13. Jang Y, Lee Y, Kim SM, Yang YD, Jung J, Oh U. (2012) Quantitative analysis of TRP channel genes in mouse organs. Arch Pharm Res, 35 (10): 1823-30. [PMID:23139135]

14. Kato AS, Knierman MD, Siuda ER, Isaac JT, Nisenbaum ES, Bredt DS. (2012) Glutamate receptor δ2 associates with metabotropic glutamate receptor 1 (mGluR1), protein kinase Cγ, and canonical transient receptor potential 3 and regulates mGluR1-mediated synaptic transmission in cerebellar Purkinje neurons. J Neurosci, 32 (44): 15296-308. [PMID:23115168]

15. Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T et al.. (2009) Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. Proc Natl Acad Sci USA, 106 (13): 5400-5. [PMID:19289841]

16. Li HS, Xu XZ, Montell C. (1999) Activation of a TRPC3-dependent cation current through the neurotrophin BDNF. Neuron, 24 (1): 261-73. [PMID:10677043]

17. Li Y, Jia YC, Cui K, Li N, Zheng ZY, Wang YZ, Yuan XB. (2005) Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor. Nature, 434 (7035): 894-8. [PMID:15758952]

18. Lievremont JP, Bird GS, Putney Jr JW. (2005) Mechanism of inhibition of TRPC cation channels by 2-aminoethoxydiphenylborane. Mol Pharmacol, 68 (3): 758-62. [PMID:15933213]

19. Lussier MP, Cayouette S, Lepage PK, Bernier CL, Francoeur N, St-Hilaire M, Pinard M, Boulay G. (2005) MxA, a member of the dynamin superfamily, interacts with the ankyrin-like repeat domain of TRPC. J Biol Chem, 280 (19): 19393-400. [PMID:15757897]

20. Maier T, Follmann M, Hessler G, Kleemann HW, Hachtel S, Fuchs B, Weissmann N, Linz W, Schmidt T, Löhn M et al.. (2015) Discovery and pharmacological characterization of a novel potent inhibitor of diacylglycerol-sensitive TRPC cation channels. Br J Pharmacol, 172 (14): 3650-60. [PMID:25847402]

21. Miehe S, Crause P, Schmidt T, Löhn M, Kleemann HW, Licher T, Dittrich W, Rütten H, Strübing C. (2012) Inhibition of diacylglycerol-sensitive TRPC channels by synthetic and natural steroids. PLoS ONE, 7 (4): e35393. [PMID:22530015]

22. Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T et al.. (2009) A pathogenic C terminus-truncated polycystin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. J Biol Chem, 284 (49): 34400-12. [PMID:19812035]

23. Mori Y, Takada N, Okada T, Wakamori M, Imoto K, Wanifuchi H, Oka H, Oba A, Ikenaka K, Kurosaki T. (1998) Differential distribution of TRP Ca2+ channel isoforms in mouse brain. Neuroreport, 9 (3): 507-15. [PMID:9512398]

24. Ohki G, Miyoshi T, Murata M, Ishibashi K, Imai M, Suzuki M. (2000) A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart. J Biol Chem, 275 (50): 39055-60. [PMID:10984475]

25. Parenti A, De Logu F, Geppetti P, Benemei S. (2016) What is the evidence for the role of TRP channels in inflammatory and immune cells?. Br J Pharmacol, 173 (6): 953-69. [PMID:26603538]

26. Qu C, Ding M, Zhu Y, Lu Y, Du J, Miller M, Tian J, Zhu J, Xu J, Wen M et al.. (2017) Pyrazolopyrimidines as Potent Stimulators for Transient Receptor Potential Canonical 3/6/7 Channels. J Med Chem, 60 (11): 4680-4692. [PMID:28395140]

27. Riccio A, Medhurst AD, Mattei C, Kelsell RE, Calver AR, Randall AD, Benham CD, Pangalos MN. (2002) mRNA distribution analysis of human TRPC family in CNS and peripheral tissues. Brain Res Mol Brain Res, 109 (1-2): 95-104. [PMID:12531519]

28. Richter JM, Schaefer M, Hill K. (2014) Clemizole hydrochloride is a novel and potent inhibitor of transient receptor potential channel TRPC5. Mol Pharmacol, 86 (5): 514-21. [PMID:25140002]

29. Rosker C, Graziani A, Lukas M, Eder P, Zhu MX, Romanin C, Groschner K. (2004) Ca(2+) signaling by TRPC3 involves Na(+) entry and local coupling to the Na(+)/Ca(2+) exchanger. J Biol Chem, 279 (14): 13696-704. [PMID:14736881]

30. Schleifer H, Doleschal B, Lichtenegger M, Oppenrieder R, Derler I, Frischauf I, Glasnov TN, Kappe CO, Romanin C, Groschner K. (2012) Novel pyrazole compounds for pharmacological discrimination between receptor-operated and store-operated Ca(2+) entry pathways. Br J Pharmacol, 167 (8): 1712-22. [PMID:22862290]

31. Seo K, Rainer PP, Shalkey Hahn V, Lee DI, Jo SH, Andersen A, Liu T, Xu X, Willette RN, Lepore JJ et al.. (2014) Combined TRPC3 and TRPC6 blockade by selective small-molecule or genetic deletion inhibits pathological cardiac hypertrophy. Proc Natl Acad Sci USA, 111 (4): 1551-6. [PMID:24453217]

32. Sinkins WG, Goel M, Estacion M, Schilling WP. (2004) Association of immunophilins with mammalian TRPC channels. J Biol Chem, 279 (33): 34521-9. [PMID:15199065]

33. Takumida M, Anniko M. (2009) Expression of canonical transient receptor potential channel (TRPC) 1-7 in the mouse inner ear. Acta Otolaryngol, 129 (12): 1351-8. [PMID:19922081]

34. Washburn DG, Holt DA, Dodson J, McAtee JJ, Terrell LR, Barton L, Manns S, Waszkiewicz A, Pritchard C, Gillie DJ et al.. (2013) The discovery of potent blockers of the canonical transient receptor channels, TRPC3 and TRPC6, based on an anilino-thiazole pharmacophore. Bioorg Med Chem Lett, 23 (17): 4979-84. [PMID:23886683]

35. Xu X, Lozinskaya I, Costell M, Lin Z, Ball JA, Bernard R, Behm DJ, Marino JP, Schnackenberg CG. (2013) Characterization of Small Molecule TRPC3 and TRPC6 agonist and Antagonists. Biophys J, 104 (2): 454a Meeting abstract. DOI: 10.1016/j.bpj.2012.11.2513

36. Yuan JP, Zeng W, Huang GN, Worley PF, Muallem S. (2007) STIM1 heteromultimerizes TRPC channels to determine their function as store-operated channels. Nat Cell Biol, 9 (6): 636-45. [PMID:17486119]

37. Zagranichnaya TK, Wu X, Villereal ML. (2005) Endogenous TRPC1, TRPC3, and TRPC7 proteins combine to form native store-operated channels in HEK-293 cells. J Biol Chem, 280 (33): 29559-69. [PMID:15972814]

38. Zhu X, Jiang M, Peyton M, Boulay G, Hurst R, Stefani E, Birnbaumer L. (1996) trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry. Cell, 85 (5): 661-71. [PMID:8646775]

39. Zitt C, Obukhov AG, Strübing C, Zobel A, Kalkbrenner F, Lückhoff A, Schultz G. (1997) Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J Cell Biol, 138 (6): 1333-41. [PMID:9298988]

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