TRPM3 | Transient Receptor Potential channels | IUPHAR/BPS Guide to PHARMACOLOGY

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TRPM3

Target not currently curated in GtoImmuPdb

Target id: 495

Nomenclature: TRPM3

Family: Transient Receptor Potential channels

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 0 1554 9q21.11 TRPM3 transient receptor potential cation channel subfamily M member 3 3,8,11
Mouse 6 1 1709 19 B Trpm3 transient receptor potential cation channel, subfamily M, member 3 14
Rat 6 0 1721 1q51 Trpm3 transient receptor potential cation channel
Previous and Unofficial Names
LTRPC3 | MLSN2 | GON-2 | transient receptor potential cation channel
Database Links
CATH/Gene3D
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
Not determined
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
calmodulin 14,26
Functional Characteristics
TRPM31235: γ = 83 pS (Na+ current), 65 pS (Ca2+ current); conducts mono and di-valent cations non-selectively (PCa/PNa = 1.6) TRPM3α1: selective for monovalent cations (PCa/PCs~0.1);
TRPM3α2: conducts mono- and di-valent cations non-selectively (PCa/PCs = 1-10);
In- and outwardly rectifying currents by co-application of pregnenolone sulphate and clotrimazole or single application of CIM0216 [5,19].
Ion Selectivity and Conductance
Species:  Human
Rank order:  Cs+ = Ca2+ > Ca2+ [73.0 - 133.0 pS]
References:  3
Ion Selectivity and Conductance Comments
Listed conductance is for TRPM31325 variant. Selectivity and conductance is heavily dependent upon splice variant and conditions [3-4,14].
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  -61.0 20.0 – 100.0 4 HEK 293 cells. Human
Inactivation  - -
Comments  TRPM31325 splice variant, activation by 10 μM D-erythrosphingosine, strong outward rectification.
Physical activators (Human)
heat (Q10 = 7.2 between 15 - 25°C), hypotonic cell swelling  [3,20-21]

Download all structure-activity data for this target as a CSV file

Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
NN-dimethyl-D-erythrosphingosine Hs Agonist - - 2x10-5 Physiological 4
Conc range: 2x10-5 M [4]
Holding voltage: Physiological
CIM2016 Hs Activation 6.1 pEC50 - - 5,18
pEC50 6.1 [5,18]
sphingosine Hs Agonist 4.9 pEC50 - Physiological 4
pEC50 4.9 (EC50 1.259x10-5 M) [4]
Holding voltage: Physiological
pregnenolone sulphate Hs - 4.9 pEC50 - - 22
pEC50 4.9 [22]
sphinganine Hs Agonist 4.7 pEC50 - - 4
pEC50 4.7 [4]
nifedipine Hs - - - - -
epipregnanolone sulphate Hs - - - - - 9
[9]
Activator Comments
TRPM3 has been shown to be constitutively open [3-4,8,14]. Also activated by hypotonic cell swelling [3]. Activation by store depletion was initially reported [8] but is disputed [3].
Gating inhibitors
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
2-APB Hs Antagonist 4.0 pIC50 - - 25
pIC50 4.0 [25]
Channel Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
isosakuranetin Hs - 6.3 pIC50 - - 15
pIC50 6.3 [15]
primidone Hs - 6.2 pIC50 - - 7
pIC50 6.2 [7]
maprotiline Hs - 5.8 pIC50 - - 7
pIC50 5.8 [7]
diclofenac Hs - 5.2 pIC50 - - 17
pIC50 5.2 [17]
liquiritigenin Hs - 5.2 pIC50 - - 15
pIC50 5.2 [15]
naringenin Hs - 5.2 pIC50 - - 15-16
pIC50 5.2 [15-16]
Gd3+ Hs Antagonist 4.0 pIC50 - - 3,8
pIC50 4.0 [3,8]
La3+ Hs Antagonist 4.0 pIC50 - - 3,8
pIC50 4.0 [3,8]
Mg2+ Mm Antagonist 2.0 pIC50 - - 14
pIC50 2.0 [14]
rosiglitazone Hs - - - - - 10
[10]
extracellular Na+ Hs - - - - -
TRPM3α2 only
mefenamic acid Hs - - - - - 6
[6]
troglitazone Hs - - - - -
pioglitazone Hs - - - - - 10
independent of PPAR-γ [10]
View species-specific channel blocker tables
Channel Blocker Comments
Extracellular monovalent cations reduced (Na+>K+) outward currents through TRPM3α2 (short pore) but not through TRPM3α1 (long pore). Extracellular divalent cations reduced (Ca2+>Mg2+) outward currents through both TRPM3α1 and TRPM3α2 [14].
Tissue Distribution
Kidney, brain, pulmonary endothelium, cultured trigeminal neurones, neuroblastoma cells, liver, pancreas, ovaries, testes, spinal cord, iris, retinal pigmented cells.
Species:  Human
Technique:  RT-PCR, Immunocytochemistry, Northern Blot, Western Blot.
References:  1-3,8,12,23-24
Brain (dentate gyrus, intermediate lateral septal nuclei, indusium giriseum, tenia tecta, epithelium of choroid plexus).
Species:  Mouse
Technique:  In situ hybridisation, Western Blot.
References:  14
Functional Assays
Two-electrode voltage clamp, patch-clamp (whole-cell and single-channel recordings).
Species:  Human
Tissue:  HEK 293 cells.
Response measured:  Constitutively active current, responses to cell swelling, activation by ligands.
References:  3,8
Physiological Functions
Probable role in osmosensation and renal Ca2+ homeostasis, mechanosensor, cation homeostasis in cerebrospinal fluid.
Species:  Human
Tissue:  Kidney, Brain.
References:  3,8
Clinically-Relevant Mutations and Pathophysiology
Disease:  Amyotrophic lateral sclerosis with frontotemporal dementia
Comments: 
References:  8,13
Disease:  Cataract 26, multiple types; CTRCT26
OMIM: 605749
Comments: 
References:  8,13
Disease:  Hemophagocytic lymphohistiocytosis
Synonyms: Hemophagocytic syndrome [Orphanet: ORPHA158032]
Disease Ontology: DOID:0050120
OMIM: 603552
Orphanet: ORPHA158032
Comments: 
References:  8,13
Disease:  Nephronophthisis 2; NPHP2
Synonyms: Nephronophthisis [Disease Ontology: DOID:12712]
Disease Ontology: DOID:12712
OMIM: 602088
Comments: 
References:  8,13

References

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1. Bollimuntha S, Ebadi M, Singh BB. (2006) TRPC1 protects human SH-SY5Y cells against salsolinol-induced cytotoxicity by inhibiting apoptosis. Brain Res., 1099 (1): 141-9. [PMID:16765919]

2. Fantozzi I, Zhang S, Platoshyn O, Remillard CV, Cowling RT, Yuan JX. (2003) Hypoxia increases AP-1 binding activity by enhancing capacitative Ca2+ entry in human pulmonary artery endothelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 285 (6): L1233-45. [PMID:12909593]

3. Grimm C, Kraft R, Sauerbruch S, Schultz G, Harteneck C. (2003) Molecular and functional characterization of the melastatin-related cation channel TRPM3. J. Biol. Chem., 278 (24): 21493-501. [PMID:12672799]

4. Grimm C, Kraft R, Schultz G, Harteneck C. (2005) Activation of the melastatin-related cation channel TRPM3 by D-erythro-sphingosine [corrected]. Mol. Pharmacol., 67 (3): 798-805. [PMID:15550678]

5. Held K, Kichko T, De Clercq K, Klaassen H, Van Bree R, Vanherck JC, Marchand A, Reeh PW, Chaltin P, Voets T et al.. (2015) Activation of TRPM3 by a potent synthetic ligand reveals a role in peptide release. Proc. Natl. Acad. Sci. U.S.A., 112 (11): E1363-72. [PMID:25733887]

6. Klose C, Straub I, Riehle M, Ranta F, Krautwurst D, Ullrich S, Meyerhof W, Harteneck C. (2011) Fenamates as TRP channel blockers: mefenamic acid selectively blocks TRPM3. Br. J. Pharmacol., 162 (8): 1757-69. [PMID:21198543]

7. Krügel U, Straub I, Beckmann H, Schaefer M. (2017) Primidone inhibits TRPM3 and attenuates thermal nociception in vivo. Pain, 158 (5): 856-867. [PMID:28106668]

8. Lee N, Chen J, Sun L, Wu S, Gray KR, Rich A, Huang M, Lin JH, Feder JN, Janovitz EB, Levesque PC, Blanar MA. (2003) Expression and characterization of human transient receptor potential melastatin 3 (hTRPM3). J. Biol. Chem., 278 (23): 20890-7. [PMID:12672827]

9. Majeed Y, Agarwal AK, Naylor J, Seymour VA, Jiang S, Muraki K, Fishwick CW, Beech DJ. (2010) Cis-isomerism and other chemical requirements of steroidal agonists and partial agonists acting at TRPM3 channels. Br. J. Pharmacol., 161 (2): 430-41. [PMID:20735426]

10. Majeed Y, Bahnasi Y, Seymour VA, Wilson LA, Milligan CJ, Agarwal AK, Sukumar P, Naylor J, Beech DJ. (2011) Rapid and contrasting effects of rosiglitazone on transient receptor potential TRPM3 and TRPC5 channels. Mol. Pharmacol., 79 (6): 1023-30. [PMID:21406603]

11. Nagase T, Kikuno R, Nakayama M, Hirosawa M, Ohara O. (2000) Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res., 7 (4): 273-81. [PMID:10997877]

12. Nealen ML, Gold MS, Thut PD, Caterina MJ. (2003) TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat. J. Neurophysiol., 90 (1): 515-20. [PMID:12634279]

13. Nilius B, Owsianik G, Voets T, Peters JA. (2007) Transient receptor potential cation channels in disease. Physiol. Rev., 87 (1): 165-217. [PMID:17237345]

14. Oberwinkler J, Lis A, Giehl KM, Flockerzi V, Philipp SE. (2005) Alternative splicing switches the divalent cation selectivity of TRPM3 channels. J. Biol. Chem., 280 (23): 22540-8. [PMID:15824111]

15. Straub I, Krügel U, Mohr F, Teichert J, Rizun O, Konrad M, Oberwinkler J, Schaefer M. (2013) Flavanones that selectively inhibit TRPM3 attenuate thermal nociception in vivo. Mol. Pharmacol., 84 (5): 736-50. [PMID:24006495]

16. Straub I, Mohr F, Stab J, Konrad M, Philipp SE, Oberwinkler J, Schaefer M. (2013) Citrus fruit and fabacea secondary metabolites potently and selectively block TRPM3. Br. J. Pharmacol., 168 (8): 1835-50. [PMID:23190005]

17. Suzuki H, Sasaki E, Nakagawa A, Muraki Y, Hatano N, Muraki K. (2016) Diclofenac, a nonsteroidal anti-inflammatory drug, is an antagonist of human TRPM3 isoforms. Pharmacol Res Perspect, 4 (3): e00232. [PMID:27433342]

18. Thiel G, Rubil S, Lesch A, Guethlein LA, Rössler OG. (2017) Transient receptor potential TRPM3 channels: Pharmacology, signaling, and biological functions. Pharmacol. Res., 124: 92-99. [PMID:28720517]

19. Vriens J, Held K, Janssens A, Tóth BI, Kerselaers S, Nilius B, Vennekens R, Voets T. (2014) Opening of an alternative ion permeation pathway in a nociceptor TRP channel. Nat. Chem. Biol., 10 (3): 188-95. [PMID:24390427]

20. Vriens J, Owsianik G, Hofmann T, Philipp SE, Stab J, Chen X, Benoit M, Xue F, Janssens A, Kerselaers S et al.. (2011) TRPM3 is a nociceptor channel involved in the detection of noxious heat. Neuron, 70 (3): 482-94. [PMID:21555074]

21. Vriens J, Voets T. (2018) Sensing the heat with TRPM3. Pflugers Arch., 470 (5): 799-807. [PMID:29305649]

22. Wagner TF, Loch S, Lambert S, Straub I, Mannebach S, Mathar I, Düfer M, Lis A, Flockerzi V, Philipp SE et al.. (2008) Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic beta cells. Nat. Cell Biol., 10 (12): 1421-30. [PMID:18978782]

23. Wistow G, Bernstein SL, Ray S, Wyatt MK, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K. (2002) Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium. Mol. Vis., 8: 185-95. [PMID:12107412]

24. Wistow G, Bernstein SL, Wyatt MK, Fariss RN, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K. (2002) Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants. Mol. Vis., 8: 205-20. [PMID:12107410]

25. Xu SZ, Zeng F, Boulay G, Grimm C, Harteneck C, Beech DJ. (2005) Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltage-dependent effect. Br. J. Pharmacol., 145 (4): 405-14. [PMID:15806115]

26. Yap KL, Kim J, Truong K, Sherman M, Yuan T, Ikura M. (2000) Calmodulin target database. J. Struct. Funct. Genomics, 1 (1): 8-14. [PMID:12836676]

Contributors

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How to cite this page

David E. Clapham, Bernd Nilius, Grzegorz Owsianik.
Transient Receptor Potential channels: TRPM3. Last modified on 06/03/2019. Accessed on 24/08/2019. IUPHAR/BPS Guide to PHARMACOLOGY, http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=495.