ChEMBL ligand: CHEMBL4297454 (Abbv-744, ABBV-744)
bromodomain containing 2/Bromodomain-containing protein 2 in Human [ChEMBL: CHEMBL1293289] [GtoPdb: 1944] [UniProtKB: P25440] There should be some charts here, you may need to enable JavaScript!
bromodomain containing 3/Bromodomain-containing protein 3 in Human [ChEMBL: CHEMBL1795186] [GtoPdb: 2725] [UniProtKB: Q15059] There should be some charts here, you may need to enable JavaScript!
bromodomain containing 4/Bromodomain-containing protein 4 in Human [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885] There should be some charts here, you may need to enable JavaScript!
bromodomain testis associated/Bromodomain testis-specific protein in Human [ChEMBL: CHEMBL1795185] [GtoPdb: 2729] [UniProtKB: Q58F21] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
bromodomain containing 2/Bromodomain-containing protein 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293289] [GtoPdb: 1944] [UniProtKB: P25440]
ChEMBL	Inhibition of N-terminal His6-tagged human BRD2 BD1 (73 to 194 residues) after 1 hr by TR-FRET assay	B	5.93	pKi	1162	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of N-terminal His6-tagged human BRD2 BD2 (348 to 455 residues) after 1 hr by TR-FRET assay	B	8.34	pKi	4.6	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of C-terminal His6-tagged BRD2 BD2 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 hrs by HTRF assay	B	9.04	pIC50	0.92	nM	IC50	J Med Chem (2022) 65: 5760-5799 [PMID:35333526]
bromodomain containing 3/Bromodomain-containing protein 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795186] [GtoPdb: 2725] [UniProtKB: Q15059]
ChEMBL	Inhibition of BRD3 BD1 (unknown origin) after 1 hr by TR-FRET assay	B	5.5	pKi	3140	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of BRD3 BD2 (unknown origin) after 1 hr by TR-FRET assay	B	8.31	pKi	4.9	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of C-terminal His6-tagged BRD3 BD2 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 hrs by HTRF assay	B	9.06	pIC50	0.88	nM	IC50	J Med Chem (2022) 65: 5760-5799 [PMID:35333526]
bromodomain containing 4/Bromodomain-containing protein 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885]
ChEMBL	Binding affinity to BRD4 BD1 (unknown origin) assessed as dissociation constant by Discover X assay	B	5.48	pKd	3300	nM	Kd	J Med Chem (2021) 64: 10497-10511 [PMID:34236185]
ChEMBL	Inhibition of BRD4-BD1 (unknown origin) assessed as dissociation constant by TR-FRET method	B	6.28	pKd	520	nM	Kd	J Med Chem (2022) 65: 5660-5674 [PMID:35348328]
ChEMBL	Binding affinity to human partial length BRD4 BD2 expressed in bacterial expression system assessed as dissociation constant by BROMOscan assay	B	8.68	pKd	2.1	nM	Kd	J Med Chem (2021) 64: 10497-10511 [PMID:34236185]
ChEMBL	Inhibition of BRD4-BD2 (unknown origin) assessed as dissociation constant by TR-FRET method	B	8.8	pKd	1.6	nM	Kd	J Med Chem (2022) 65: 5660-5674 [PMID:35348328]
ChEMBL	Inhibition of human N-terminal His6 tagged BRD4 BD1 (57 to 168) residues expressed in Escherichia coli BL21(DE3) cells after 1 hr by TR-FRET assay	B	6.28	pKi	520	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of BRD4 BD2 (unknown origin)	B	6.28	pKi	520	nM	Ki	J Med Chem (2021) 64: 2419-2435 [PMID:33616410]
ChEMBL	Inhibition of human N-terminal His6 tagged BRD4 BD2 (352 to 457) residues expressed in Escherichia coli BL21(DE3) cells after 1 hr by TR-FRET assay	B	8.8	pKi	1.6	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of BRD4 BD1 (unknown origin)	B	8.8	pKi	1.6	nM	Ki	J Med Chem (2021) 64: 2419-2435 [PMID:33616410]
ChEMBL	Inhibition of NanoLuc-tagged BRD4 BD1 (unknown origin) expressed in human HeLa cells after 2 hrs by NanoBRET assay	B	4.68	pIC50	20700	nM	IC50	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of His-tagged BRD4 bromodomain 1 (unknown origin) using [Lys (5,8,12,16) Ac] H4(1-21) as substrate by fluorescence based assay	B	6.48	pIC50	327.5	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of BRD4-BD1 (unknown origin) preincubated for 30 mins followed by substrate addition and measured after 3 hr by HTRF assay	B	6.52	pIC50	299.75	nM	IC50	Eur J Med Chem (2022) 236: 114259-114259 [PMID:35395439]
ChEMBL	Inhibition of C-terminal His6-tagged BRD4 BD1 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 hrs by HTRF assay	B	6.62	pIC50	238	nM	IC50	J Med Chem (2022) 65: 5760-5799 [PMID:35333526]
ChEMBL	Inhibition of NanoLuc-tagged BRD4 BD2 (unknown origin) expressed in human HeLa cells after 2 hrs by NanoBRET assay	B	7.56	pIC50	27.5	nM	IC50	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of His-tagged BRD4 bromodomain 2 (unknown origin) using [Lys (5,8,12,16) Ac] H4(1-21) as substrate by fluorescence based assay	B	8.48	pIC50	3.3	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of BRD4-BD2 (unknown origin) preincubated for 30 mins followed by substrate addition and measured after 3 hr by HTRF	B	8.75	pIC50	1.78	nM	IC50	Eur J Med Chem (2022) 236: 114259-114259 [PMID:35395439]
ChEMBL	Inhibition of C-terminal His6-tagged BRD4 BD2 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 hrs by HTRF assay	B	9.19	pIC50	0.65	nM	IC50	J Med Chem (2022) 65: 5760-5799 [PMID:35333526]
bromodomain testis associated/Bromodomain testis-specific protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795185] [GtoPdb: 2729] [UniProtKB: Q58F21]
ChEMBL	Inhibition of BRDT BD1 (unknown origin) after 1 hr by TR-FRET assay	B	6.04	pKi	917	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
ChEMBL	Inhibition of BRDT BD2 (unknown origin) after 1 hr by TR-FRET assay	B	9	pKi	1	nM	Ki	J Med Chem (2020) 63: 5585-5623 [PMID:32324999]
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Inhibition of HDAC1 (unknown origin) by fluorescence based assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
histone deacetylase 6/Histone deacetylase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7]
ChEMBL	Inhibition of HDAC6 (unknown origin) by fluorescence based assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]