ChEMBL ligand: CHEMBL22 (BW 56-72, BW-56-72, Infectotrimet, Ipral, Ledatrim, Monotrim, NIH-204, NSC-106568, Polytrim, Primsol, Proloprim, Proloprin, Syraprim, TCMDC-125538, Tiempe, Trimethoprim, Trimethoprime, Trimethoprimum, Trimetoprima, Trimogal, Trimopan, Trimpex, Trimpex 200, Triprimix 200, Wellcoprim)
Bacterial dihydrofolate reductase in Bacteria [ChEMBL: CHEMBL2364669] [UniProtKB: P0ABQ4] There should be some charts here, you may need to enable JavaScript!
Bifunctional dihydrofolate reductase-thymidylate synthase in Cryptosporidium parvum [ChEMBL: CHEMBL2366489] [UniProtKB: Q27552] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase in Neisseria gonorrhoeae [ChEMBL: CHEMBL3565] [UniProtKB: P04174] Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4] Dihydrofolate reductase in S.aureus [ChEMBL: CHEMBL1681620] [UniProtKB: P0A017] Dihydrofolate reductase in Bacillus anthracis [ChEMBL: CHEMBL5270] [UniProtKB: Q81R22] Dihydrofolate reductase in Lactobacillus casei [ChEMBL: CHEMBL2902] [UniProtKB: P00381] Dihydrofolate reductase in Mycobacterium avium [ChEMBL: CHEMBL5457] [UniProtKB: O30463] Dihydrofolate reductase in Mycobacterium tuberculosis [ChEMBL: CHEMBL6065] [UniProtKB: P9WNX1] Dihydrofolate reductase in Pneumocystis carinii [ChEMBL: CHEMBL1926] [UniProtKB: P16184] Dihydrofolate reductase in Candida albicans [ChEMBL: CHEMBL2329] [UniProtKB: P22906] Dihydrofolate reductase in Leishmania major [ChEMBL: CHEMBL4614] [UniProtKB: P07382] Dihydrofolate reductase in Toxoplasma gondii [ChEMBL: CHEMBL2425] [UniProtKB: Q07422] Dihydrofolate reductase in Plasmodium berghei str. ANKA [ChEMBL: CHEMBL3963] [UniProtKB: Q27713] Dihydrofolate reductase in Plasmodium falciparum K1 [ChEMBL: CHEMBL1939] [UniProtKB: P13922] dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] dihydrofolate reductase/Dihydrofolate reductase in Mouse [ChEMBL: CHEMBL4564] [GtoPdb: 2603] [UniProtKB: P00375] dihydrofolate reductase/Dihydrofolate reductase in Rat [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2] Dihydrofolate reductase in Pneumocystis jirovecii [ChEMBL: CHEMBL2057] [UniProtKB: Q9UUP5] Dihydrofolate reductase in Salmonella enterica subsp. enterica serovar Typhi [ChEMBL: CHEMBL4888] [UniProtKB: Q8Z9J9] Dihydrofolate reductase in Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [ChEMBL: CHEMBL4296314] [UniProtKB: Q54801] Dihydrofolate reductase in Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcuslactis) [ChEMBL: CHEMBL4012] [UniProtKB: Q59487] Dihydrofolate reductase in Saccharomyces cerevisiae S288c [ChEMBL: CHEMBL2576] [UniProtKB: P07807] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase type 1 in Escherichia coli [ChEMBL: CHEMBL2627] [UniProtKB: P00382] There should be some charts here, you may need to enable JavaScript!
NH(3)-dependent NAD(+) synthetase in Bacillus subtilis (strain 168) [ChEMBL: CHEMBL4615] [UniProtKB: P08164] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Organic cation transporter 1/Solute carrier family 22 member 1 in Human [ChEMBL: CHEMBL5685] [GtoPdb: 1019] [UniProtKB: O15245] There should be some charts here, you may need to enable JavaScript!
TAAR5/Trace amine-associated receptor 5 in Mouse [ChEMBL: CHEMBL3784908] [GtoPdb: 170] [UniProtKB: Q5QD14] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Bacterial dihydrofolate reductase in Bacteria (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2364669] [UniProtKB: P0ABQ4]
ChEMBL	In vitro inhibition of Pneumocystis carinii (Pc) dihydrofolate reductase.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2002) 45: 233-241 [PMID:11754594]
ChEMBL	Inhibitory activity against Dihydrofolate reductase in Neisseria gonorrhoeae	B	6.35	pIC50	450	nM	IC50	J Med Chem (1989) 32: 1927-1935 [PMID:2502632]
Bifunctional dihydrofolate reductase-thymidylate synthase in Cryptosporidium parvum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366489] [UniProtKB: Q27552]
ChEMBL	Inhibition of Cryptosporidium hominis DHFR	B	4.85	pIC50	14000	nM	IC50	Bioorg Med Chem Lett (2006) 16: 4366-4370 [PMID:16750361]
ChEMBL	Inhibition of Cryptosporidium parvum recombinant DHFR using dihydrofolate as substrate incubated for 5 min prior to substrate addition by spectrophotometric assay	B	5.4	pIC50	4000	nM	IC50	Med Chem Res (2008) 17: 541-563
Dihydrofolate reductase in Neisseria gonorrhoeae (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3565] [UniProtKB: P04174]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) of Neisseria gonorrhoea	B	6.35	pIC50	450	nM	IC50	J Med Chem (1987) 30: 348-356 [PMID:3100802]
ChEMBL	Inhibitory activity against Neisseria gonorrhoeae dihydrofolate reductase at 10e8 M	B	6.35	pIC50	450	nM	IC50	J Med Chem (1991) 34: 550-555 [PMID:1995876]
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4]
ChEMBL	Inhibition constant against Dihydrofolate reductase	B	8.42	pKi	3.8	nM	Ki	Bioorg Med Chem Lett (2003) 13: 2493-2496 [PMID:12852950]
ChEMBL	Inhibition constant against binding of Escherichia coli dihydrofolate reductase	B	8.87	pKi	1.35	nM	Ki	J Med Chem (1988) 31: 1396-1406 [PMID:3290487]
ChEMBL	Antibacterial activity against Escherichia coli	B	8.89	pKi	1.3	nM	Ki	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Binding affinity against Dihydrofolate reductase of Escherichia coli	B	8.89	pKi	1.3	nM	Ki	J Med Chem (1985) 28: 303-311 [PMID:3973902]
ChEMBL	Binding affinity for Escherichia coli Dihydrofolate reductase	B	8.89	pKi	1.3	nM	Ki	J Med Chem (1982) 25: 1120-1122 [PMID:6754932]
ChEMBL	Apparent binding affinity against Dihydrofolate reductase in Escherichia coli	B	8.89	pKi	1.3	nM	Ki	J Med Chem (1989) 32: 1942-1949 [PMID:2666668]
ChEMBL	Inhibition of recombinant Escherichia coli DHFR expressed in Escherichia coli BL21(DE3) cells assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition	B	9.66	pKi	0.22	nM	Ki	ACS Med Chem Lett (2016) 7: 692-696 [PMID:27437079]
ChEMBL	Inhibition of dihydrofolate reductase from pneumocystis carinii.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2003) 46: 5074-5082 [PMID:14584957]
ChEMBL	Inhibition of dihydrofolate reductase from Toxoplasma gondii.	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2003) 46: 5074-5082 [PMID:14584957]
ChEMBL	Inhibitory activity against recombinant Escherichia coli dihydrofolate reductase	B	6	pIC50	1000	nM	IC50	J Med Chem (2001) 44: 1993-2003 [PMID:11384244]
ChEMBL	Inhibition of Escherichia coli DHFR incubated for 10 mins in presence of NADPH by absorbance based analysis	B	7.25	pIC50	56.6	nM	IC50	J Med Chem (2023) 66: 13946-13967 [PMID:37698518]
ChEMBL	Inhibition of Escherichia coli Dihydrofolate reductase in presence of 100 uM Dihydrofolate reductase	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem Lett (2003) 13: 2493-2496 [PMID:12852950]
ChEMBL	Inhibitory activity against Escherichia coli (ec) Dihydrofolate reductase	B	7.7	pIC50	20	nM	IC50	J Med Chem (2002) 45: 5173-5181 [PMID:12408727]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	7.7	pIC50	20	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibition of Escherichia coli DHFR	B	7.7	pIC50	20	nM	IC50	J Med Chem (2010) 53: 1563-1578 [PMID:20092323]
ChEMBL	Inhibition of Escherichia coli Dihydrofolate reductase in presence of 30 uM Dihydrofolate reductase	B	7.74	pIC50	18	nM	IC50	Bioorg Med Chem Lett (2003) 13: 2493-2496 [PMID:12852950]
ChEMBL	Inhibitory activity against recombinant Dihydrofolate reductase from Escherichia coli (ecDHFR)	B	7.74	pIC50	18	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
ChEMBL	Compound was evaluated as inhibitor of Escherichia coli Dihydrofolate reductase	B	7.82	pIC50	15	nM	IC50	J Med Chem (2003) 46: 591-600 [PMID:12570380]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Escherichia coli DHFR	B	8	pIC50	10	nM	IC50	J Med Chem (2008) 51: 5789-5797 [PMID:18800768]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	8	pIC50	10	nM	IC50	J Med Chem (2009) 52: 4892-4902 [PMID:19719239]
ChEMBL	Inhibitory activity against Escherichia coli dihydrofolate reductase at 10e8 M	B	8.15	pIC50	7	nM	IC50	J Med Chem (1991) 34: 550-555 [PMID:1995876]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) from Escherichia coli	B	8.15	pIC50	7	nM	IC50	J Med Chem (1987) 30: 348-356 [PMID:3100802]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase (DHFR) enzyme by Iclaprim	B	8.15	pIC50	7	nM	IC50	Bioorg Med Chem Lett (2003) 13: 4217-4221 [PMID:14623005]
ChEMBL	In vitro inhibition of dihydrofolate reductase of Escherichia coli	B	8.22	pIC50	6	nM	IC50	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Compound was tested for inhibition of Escherichia coli Dihydrofolate reductase.	B	8.23	pIC50	5.89	nM	IC50	J Med Chem (1996) 39: 4825-4832 [PMID:8941396]
ChEMBL	Inhibitory activity against Dihydrofolate reductase obtained from E. coli; Range 0.005-0.007	B	8.3	pIC50	5	nM	IC50	J Med Chem (1991) 34: 1383-1394 [PMID:2016714]
ChEMBL	Inhibitory activity against Dihydrofolate reductase enzyme purified from Escherichia coli	B	8.3	pIC50	5	nM	IC50	J Med Chem (1980) 23: 379-384 [PMID:6991692]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) enzyme from Escherichia coli	B	8.3	pIC50	5	nM	IC50	J Med Chem (1987) 30: 1998-2004 [PMID:3312605]
ChEMBL	Inhibition against dihydrofolate reductase enzyme of Escherichia coli	B	8.3	pIC50	5	nM	IC50	J Med Chem (1980) 23: 535-541 [PMID:6991695]
ChEMBL	In vitro inhibition of Escherichia coli dihydrofolate reductase.	B	8.3	pIC50	5	nM	IC50	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Inhibitory activity against Dihydrofolate reductase in Escherichia coli	B	8.3	pIC50	5	nM	IC50	J Med Chem (1989) 32: 1936-1942 [PMID:2502633]
ChEMBL	Inhibitory activity against Dihydrofolate reductase of Escherichia coli	B	8.3	pIC50	5	nM	IC50	J Med Chem (1981) 24: 933-941 [PMID:7035668]
Dihydrofolate reductase in S.aureus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1681620] [UniProtKB: P0A017]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus recombinant DHFR F98Y/H149R double mutant expressed in Escherichia coli BL21 (DE3) cells assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	5.76	pKi	1729	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus recombinant DHFR H30N/F98Y double mutant expressed in Escherichia coli BL21 (DE3) cells assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	6.23	pKi	595.1	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus recombinant DHFR H149R mutant expressed in Escherichia coli BL21 (DE3) cells assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	6.62	pKi	240.8	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Inhibition of TMP-resistant Staphylococcus aureus DHFR F99Y mutant assessed as oxidation of NADPH using dihydrofolate as substrate pre-incubated for 10 mins before substrate addition by spectrophotometry	B	7.05	pKi	90	nM	Ki	Bioorg Med Chem Lett (2011) 21: 5171-5176 [PMID:21831637]
ChEMBL	Inhibition of Staphylococcus aureus DHFR F98Y mutant by MTS assay	B	7.27	pKi	53.6	nM	Ki	Antimicrob Agents Chemother (2010) 54: 3825-3833 [PMID:20606069]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus recombinant DHFR F98Y mutant expressed in Escherichia coli BL21 (DE3) cells assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	7.83	pKi	14.68	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus recombinant DHFR H30N mutant expressed in Escherichia coli BL21 (DE3) cells assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	8.16	pKi	6.89	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Antibacterial activity against Staphylococcus aureus	B	8.29	pKi	5.1	nM	Ki	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Inhibition of Staphylococcus aureus wild type recombinant DHFR by MTS assay	B	8.36	pKi	4.4	nM	Ki	Antimicrob Agents Chemother (2010) 54: 3825-3833 [PMID:20606069]
ChEMBL	Inhibition of TMP/methicillin-resistant Staphylococcus aureus wild type DHFR assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihydrofolate substrate addition	B	8.46	pKi	3.43	nM	Ki	J Med Chem (2016) 59: 6493-6500 [PMID:27308944]
ChEMBL	Inhibition of recombinant Staphylococcus aureus DHFR expressed in Escherichia coli BL21(DE3) cells assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition	B	8.46	pKi	3.43	nM	Ki	ACS Med Chem Lett (2016) 7: 692-696 [PMID:27437079]
ChEMBL	Inhibition of Staphylococcus aureus DHFR assessed as oxidation of NADPH using dihydrofolate as substrate pre-incubated for 10 mins before substrate addition by spectrophotometry	B	8.91	pKi	1.24	nM	Ki	Bioorg Med Chem Lett (2011) 21: 5171-5176 [PMID:21831637]
ChEMBL	Inhibition of Staphylococcus aureus DHFR using dihydrofolate as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric analysis in presence of NADPH	B	8.92	pKi	1.2	nM	Ki	J Med Chem (2014) 57: 651-668 [PMID:24428639]
ChEMBL	Inhibition of trimethoprim-resistant Staphylococcus aureus DHFR F98Y mutant	B	4.35	pIC50	45000	nM	IC50	J Med Chem (2007) 50: 4374-4381 [PMID:17696333]
ChEMBL	Tested for inhibition of TMP-Resistant Dihydrofolate reductase from Staphylococcus aureus 157/4696.	B	4.72	pIC50	19000	nM	IC50	J Med Chem (2003) 46: 2304-2312 [PMID:12773035]
ChEMBL	Inhibition of trimethoprim-resistant Staphylococcus aureus DfrB type dihydrofolate reductase F98Y mutant	B	4.99	pIC50	10243	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3620-3627 [PMID:19546364]
ChEMBL	Inhibition of methicillin-resistant Staphylococcus aureus DHFR F98Y mutant	B	5.77	pIC50	1700	nM	IC50	Medchemcomm (2013) 4: 908-915
ChEMBL	Inhibition of Staphylococcus aureus DHFR F98Y mutant by MTS assay	B	6.27	pIC50	539.7	nM	IC50	Antimicrob Agents Chemother (2010) 54: 3825-3833 [PMID:20606069]
ChEMBL	Antibacterial activity against TMP-Resistant Dihydrofolate reductase from Staphylococcus aureus 157/4696	B	7.57	pIC50	27	nM	IC50	J Med Chem (2003) 46: 2304-2312 [PMID:12773035]
ChEMBL	Inhibition of methicillin-resistant Staphylococcus aureus DHFR	B	7.64	pIC50	23	nM	IC50	Medchemcomm (2013) 4: 908-915
ChEMBL	Inhibition of Staphylococcus aureus wild type recombinant DHFR by MTS assay	B	7.84	pIC50	14.4	nM	IC50	Antimicrob Agents Chemother (2010) 54: 3825-3833 [PMID:20606069]
ChEMBL	Inhibition of Staphylococcus aureus dihydrofolate reductase (DHFR) enzyme by Iclaprim	B	8.15	pIC50	7	nM	IC50	Bioorg Med Chem Lett (2003) 13: 4217-4221 [PMID:14623005]
Dihydrofolate reductase in Bacillus anthracis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5270] [UniProtKB: Q81R22]
ChEMBL	Inhibition of wild type Bacillus anthracis recombinant DHFR	B	4.62	pKi	24100	nM	Ki	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
ChEMBL	Inhibition of Bacillus anthracis recombinant DHFR F96I mutant	B	4.86	pKi	13700	nM	Ki	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
ChEMBL	Inhibition of Bacillus anthracis recombinant DHFR Y102F mutant	B	6.32	pKi	480	nM	Ki	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
ChEMBL	Inhibition of Bacillus anthracis DHFR	B	4.11	pIC50	77000	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3065-3073 [PMID:19364848]
ChEMBL	Inhibition of wild type Bacillus anthracis recombinant DHFR	B	4.15	pIC50	71400	nM	IC50	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
ChEMBL	Inhibition of Bacillus anthracis recombinant DHFR expressed in mouse M15 cells assessed as rate of enzyme-dependent NADPH oxidation	B	4.15	pIC50	71000	nM	IC50	J Med Chem (2008) 51: 7532-7540 [PMID:19007108]
ChEMBL	Inhibition of Bacillus anthracis recombinant DHFR F96I mutant	B	5.24	pIC50	5760	nM	IC50	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
ChEMBL	Inhibition of Bacillus anthracis recombinant DHFR Y102F mutant	B	5.42	pIC50	3780	nM	IC50	J Med Chem (2010) 53: 7327-7336 [PMID:20882962]
Dihydrofolate reductase in Lactobacillus casei (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2902] [UniProtKB: P00381]
ChEMBL	Inhibitory activity against Lactobacillus casei dihydrofolate reductase	B	6.88	pKi	131.83	nM	Ki	J Med Chem (1989) 32: 1895-1905 [PMID:2502631]
ChEMBL	Compound was evaluated as inhibitor of Lactobacillus casei Dihydrofolate reductase	B	6.21	pIC50	620	nM	IC50	J Med Chem (2003) 46: 591-600 [PMID:12570380]
ChEMBL	Inhibition of Lactobacillus casei DHFR	B	6.21	pIC50	620	nM	IC50	J Med Chem (2007) 50: 3283-3289 [PMID:17569517]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Lactobacillus casei	B	6.99	pIC50	103	nM	IC50	J Med Chem (1995) 38: 1778-1785 [PMID:7752201]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Lactobacillus casei (lcDHFR)	B	7.27	pIC50	54	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
Dihydrofolate reductase in Mycobacterium avium (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5457] [UniProtKB: O30463]
ChEMBL	Inhibition of Mycobacterium avium DHFR	B	3.52	pIC50	300000	nM	IC50	J Med Chem (2007) 50: 3046-3053 [PMID:17552508]
ChEMBL	Concentration required to inhibit the Mycobacterium avium Dihydrofolate reductase by 50% was determined; Range: 260-350	B	6.52	pIC50	300	nM	IC50	J Med Chem (2004) 47: 2475-2485 [PMID:15115391]
ChEMBL	Inhibition of Mycobacterium avium DHFR at 37 degC by spectrophotometry	B	6.52	pIC50	300	nM	IC50	Bioorg Med Chem (2010) 18: 953-961 [PMID:20056546]
ChEMBL	Inhibition of Dihydrohydrofolate reductase(DHFR) of Mycobacterium avium	B	6.52	pIC50	300	nM	IC50	J Med Chem (2003) 46: 1726-1736 [PMID:12699390]
ChEMBL	Inhibitory concentration against Mycobacterium avium DHFR	B	6.52	pIC50	300	nM	IC50	J Med Chem (2005) 48: 4420-4431 [PMID:15974594]
ChEMBL	Inhibitory concentration against dihydrofolate reductase of Mycobacterium avium	B	6.52	pIC50	300	nM	IC50	J Med Chem (2004) 47: 1475-1486 [PMID:14998335]
ChEMBL	Inhibition of partially purified dihydrofolate reductase (DHFR) from Mycobacterium avium (Ma)	B	6.72	pIC50	190	nM	IC50	J Med Chem (2002) 45: 233-241 [PMID:11754594]
ChEMBL	Inhibitory activity against DHFR (Dihydrofolate reductase) from Mycobacterium avium	B	6.72	pIC50	190	nM	IC50	J Med Chem (1999) 42: 4853-4860 [PMID:10579848]
ChEMBL	Inhibition of Mycobacterium avium DHFR	B	6.72	pIC50	190	nM	IC50	J Med Chem (2007) 50: 3283-3289 [PMID:17569517]
Dihydrofolate reductase in Mycobacterium tuberculosis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6065] [UniProtKB: P9WNX1]
ChEMBL	Inhibition of recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis	B	4.06	pIC50	88000	nM	IC50	Bioorg Med Chem (2020) 28: 115600-115600 [PMID:32631571]
Dihydrofolate reductase in Pneumocystis carinii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1926] [UniProtKB: P16184]
ChEMBL	Binding affinity was reported with purified recombinant Pneumocystis carinii Dihydrofolate reductase	B	6.82	pKi	152	nM	Ki	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibition of dihydrofolate reductase (DHFR) from Pneumocystis carinii.	B	6.82	pKi	151.36	nM	Ki	J Med Chem (1995) 38: 967-972 [PMID:7699713]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	1.92	pIC50	12000000	nM	IC50	J Med Chem (2007) 50: 3046-3053 [PMID:17552508]
ChEMBL	Inhibition of Pneumocystis carinii dihydrofolate reductase (DHFR) enzyme by Iclaprim	B	4.37	pIC50	43000	nM	IC50	Bioorg Med Chem Lett (2003) 13: 4217-4221 [PMID:14623005]
ChEMBL	Inhibitory concentration against Pneumocystis carinii dihydrofolate reductases (DHFR)	B	4.4	pIC50	40000	nM	IC50	J Med Chem (1993) 36: 3437-3443 [PMID:8230134]
ChEMBL	Inhibitory concentration against dihydrofolate reductase dihydrofolate reductase from Pneumocystis carinii.	B	4.57	pIC50	26800	nM	IC50	J Med Chem (2001) 44: 2391-2402 [PMID:11448221]
ChEMBL	Inhibitory activity against pneumocystis carinii Dihydrofolate reductase	B	4.82	pIC50	15000	nM	IC50	J Med Chem (2002) 45: 5173-5181 [PMID:12408727]
ChEMBL	Inhibitory concentration against Pneumocystis carinii DHFR	B	4.89	pIC50	13000	nM	IC50	J Med Chem (2005) 48: 4420-4431 [PMID:15974594]
ChEMBL	Inhibition of Dihydrohydrofolate reductase(DHFR) of Pneumocystis carinii	B	4.89	pIC50	13000	nM	IC50	J Med Chem (2003) 46: 1726-1736 [PMID:12699390]
ChEMBL	Concentration required to inhibit the Pneumocystis carinii Dihydrofolate reductase by 50% was determined; Range: 10000-16000	B	4.89	pIC50	13000	nM	IC50	J Med Chem (2004) 47: 2475-2485 [PMID:15115391]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	4.9	pIC50	12500	nM	IC50	J Med Chem (2013) 56: 4422-4441 [PMID:23627352]
ChEMBL	Inhibitory activity against dihydrofolate reductase from pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1998) 41: 1263-1271 [PMID:9548816]
ChEMBL	The ability to inhibit Pneumocystis carinii Dihydrofolate reductase was tested	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1999) 42: 1007-1017 [PMID:10090784]
ChEMBL	Concentration inhibiting Pneumocystis carinii dihydrofolate reductase.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 2615-2620 [PMID:7629801]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Pneumocystis carinii (pcDHFR)	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
ChEMBL	Inhibition against Dihydrofolate reductase in Pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1997) 40: 1173-1177 [PMID:9089339]
ChEMBL	Inhibition of Pneumocystis carinii Dihydrofolate Reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 1778-1785 [PMID:7752201]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) from Pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1998) 41: 4533-4541 [PMID:9804692]
ChEMBL	Inhibition of Dihydrofolate Reductase of Pneumocystis carinii.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1997) 40: 479-485 [PMID:9046338]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase (in 90 uM dihydrofolic acid)	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]
ChEMBL	Inhibition of Pneumocystis carinii (pc) Dihydrofolate reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 2158-2165 [PMID:7783147]
ChEMBL	Inhibition of Pneumocystis carinii dihydrofolate reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1998) 41: 1409-1416 [PMID:9554874]
ChEMBL	Inhibition of Dihydrofolate reductase of Pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	4.92	pIC50	12000	nM	IC50	Bioorg Med Chem Lett (2006) 16: 4366-4370 [PMID:16750361]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2007) 50: 3283-3289 [PMID:17569517]
ChEMBL	Inhibition of Pneumocystis carinii dihydrofolate reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2008) 51: 6195-6200 [PMID:18771252]
ChEMBL	Inhibition of Pneumocystis carinii DHFR using dihydrofolic acid substrate and NADPH cofactor	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibition of Pneumocystis carinii DHFR at 37 degC by spectrophotometry	B	4.92	pIC50	12000	nM	IC50	Bioorg Med Chem (2010) 18: 953-961 [PMID:20056546]
ChEMBL	Inhibitory activity against DHFR (Dihydrofolate reductase) from Pneumocystis carinii.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1999) 42: 4853-4860 [PMID:10579848]
ChEMBL	Inhibitory concentration against Dihydrofolate reductase from Pneumocystis carinii (pc)	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1997) 40: 470-478 [PMID:9046337]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Pneumocystis carinii at 37 centigrade.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Compound was tested for inhibition activity against Dihydrofolate reductase in pneumocystis carinii (Pneumocystis carinii).	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against Pneumocystis carinii Dihydrofolate reductase	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibitory concentration against dihydrofolate reductace enzyme of Pneumocystis carinii	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2004) 47: 1475-1486 [PMID:14998335]
ChEMBL	Inhibitory activity against purified DHFR (Dihydrofolate reductase) from Pneumocystis carinii.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1995) 38: 3660-3668 [PMID:7658454]
ChEMBL	The compound was tested for inhibition of dihydrofolate reductase(DHFR) from Pneumocystis carinii(pc)	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1999) 42: 2447-2455 [PMID:10395486]
ChEMBL	Concentration required to inhibit the Pneumocystis carinii Dihydrofolate reductase by 50% was determined (reported by workers at Hoffman-LaRoche)	B	7.34	pIC50	46	nM	IC50	J Med Chem (2004) 47: 2475-2485 [PMID:15115391]
Dihydrofolate reductase in Candida albicans (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2329] [UniProtKB: P22906]
ChEMBL	Inhibition of dihydrofolate reductase in Candida albicans (in vitro).	B	4.3	pIC50	50000	nM	IC50	J Med Chem (1995) 38: 3608-3616 [PMID:7658448]
ChEMBL	Inhibition of Candida albicans DHFR expressed in Escherichia coli BL21 (DE3) assessed as rate of NADPH consumption using dihydrofolate as substrate	B	4.38	pIC50	42000	nM	IC50	Bioorg Med Chem (2009) 17: 4866-4872 [PMID:19560363]
ChEMBL	Inhibition of Candida albicans DHFR	B	4.39	pIC50	41000	nM	IC50	Medchemcomm (2013) 4: 908-915
ChEMBL	Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Candida albicans	B	4.52	pIC50	30300	nM	IC50	J Med Chem (2004) 47: 240-253 [PMID:14695838]
ChEMBL	Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Mycobacterium lufu	B	6.57	pIC50	270	nM	IC50	J Med Chem (2004) 47: 240-253 [PMID:14695838]
ChEMBL	Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Escherichia coli	B	8.66	pIC50	2.2	nM	IC50	J Med Chem (2004) 47: 240-253 [PMID:14695838]
Dihydrofolate reductase in Leishmania major (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4614] [UniProtKB: P07382]
ChEMBL	Inhibition of Leishmania major DHFR assessed by NADPH oxidation measured by spectrophotometric method	B	6.92	pKi	120	nM	Ki	Eur J Med Chem (2021) 210: 112986-112986 [PMID:33187806]
ChEMBL	Inhibition of Leishmania major DHFR in promastigote stage Leishmania major using dihydrofolic acid as substrate in presence of NADPH measured after 5 days by spectrophotometric method	B	4.69	pIC50	20500	nM	IC50	Eur J Med Chem (2021) 210: 112986-112986 [PMID:33187806]
Dihydrofolate reductase in Toxoplasma gondii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2425] [UniProtKB: Q07422]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	2.55	pIC50	2800000	nM	IC50	J Med Chem (2007) 50: 3046-3053 [PMID:17552508]
ChEMBL	Inhibition of Toxoplasma gondii DHFR-TS expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 60 mins by resazurin dye based diaphorase-coupled assay	B	4.48	pIC50	33100	nM	IC50	J Med Chem (2019) 62: 1562-1576 [PMID:30624926]
ChEMBL	Inhibitory concentration against Toxoplasma gondii dihydrofolate reductases (DHFR)	B	5	pIC50	10000	nM	IC50	J Med Chem (1993) 36: 3437-3443 [PMID:8230134]
ChEMBL	Inhibition of Toxoplasma gondii DHFR-TS	B	5.1	pIC50	8000	nM	IC50	J Med Chem (2007) 50: 940-950 [PMID:17269758]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	5.17	pIC50	6800	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	5.17	pIC50	6800	nM	IC50	J Med Chem (2008) 51: 5789-5797 [PMID:18800768]
ChEMBL	Inhibition of Toxoplasma gondii dihydrofolate reductase	B	5.17	pIC50	6800	nM	IC50	J Med Chem (2009) 52: 4892-4902 [PMID:19719239]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase	B	5.43	pIC50	3700	nM	IC50	J Med Chem (2001) 44: 1993-2003 [PMID:11384244]
ChEMBL	Inhibitory activity against Toxoplasma gondii (tg) Dihydrofolate reductase (DHFR)	B	5.47	pIC50	3400	nM	IC50	J Med Chem (2002) 45: 5173-5181 [PMID:12408727]
ChEMBL	Inhibition of Toxoplasma gondii dihydrofolate reductase	B	5.54	pIC50	2900	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibitory activity against Dihydrohydrofolate reductase(DHFR) of Toxoplasma gondii	B	5.55	pIC50	2800	nM	IC50	J Med Chem (2003) 46: 1726-1736 [PMID:12699390]
ChEMBL	Concentration required to inhibit the Toxoplasma gondii Dihydrofolate reductase by 50% was determined; Range: 2400-3300	B	5.55	pIC50	2800	nM	IC50	J Med Chem (2004) 47: 2475-2485 [PMID:15115391]
ChEMBL	Inhibition of Toxoplasma gondii DHFR at 37 degC by spectrophotometry	B	5.55	pIC50	2800	nM	IC50	Bioorg Med Chem (2010) 18: 953-961 [PMID:20056546]
ChEMBL	Inhibitory concentration against dihydrofolate reductase of Toxoplasma gondii	B	5.55	pIC50	2800	nM	IC50	J Med Chem (2004) 47: 1475-1486 [PMID:14998335]
ChEMBL	Inhibitory concentration against Toxoplasma gondii DHFR	B	5.55	pIC50	2800	nM	IC50	J Med Chem (2005) 48: 4420-4431 [PMID:15974594]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	5.56	pIC50	2730	nM	IC50	J Med Chem (2007) 50: 3283-3289 [PMID:17569517]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase	B	5.56	pIC50	2730	nM	IC50	J Med Chem (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase (in 90 uM dihydrofolic acid)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1996) 39: 1438-1446 [PMID:8691474]
ChEMBL	Inhibition of Toxoplasma gondii (tc) Dihydrofolate reductase	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1995) 38: 2158-2165 [PMID:7783147]
ChEMBL	Inhibition of Toxoplasma gondii Dihydrofolate reductase	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1998) 41: 1409-1416 [PMID:9554874]
ChEMBL	Inhibition of partially purified dihydrofolate reductase (DHFR) from Toxoplasma gondii (Tg)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2002) 45: 233-241 [PMID:11754594]
ChEMBL	Inhibition of Dihydrofolate reductase of Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibition of Toxoplasma gondii dihydrofolate reductase	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2008) 51: 6195-6200 [PMID:18771252]
ChEMBL	Inhibitory activity against DHFR (Dihydrofolate reductase) from Toxoplasma gondii.	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1999) 42: 4853-4860 [PMID:10579848]
ChEMBL	Inhibitory concentration against Dihydrofolate reductase from Toxoplasma gondii (tg)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1997) 40: 470-478 [PMID:9046337]
ChEMBL	Compound was tested for inhibition activity against Dihydrofolate reductase in Toxoplasma gondii (Toxoplasma gondii)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase.	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1996) 39: 1836-1845 [PMID:8627607]
ChEMBL	Inhibitory concentration against dihydrofolate reductase DHFR from Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2001) 44: 2391-2402 [PMID:11448221]
ChEMBL	Inhibitory activity against Toxoplasma gondii Dihydrofolate reductase	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibitory activity against purified DHFR (Dihydrofolate reductase) from Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1995) 38: 3660-3668 [PMID:7658454]
ChEMBL	The compound was tested for inhibition of dihydrofolate reductase(DHFR) from Toxoplasma gondii(tg)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1999) 42: 2447-2455 [PMID:10395486]
ChEMBL	Inhibitory activity against dihydrofolate reductase from Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1998) 41: 1263-1271 [PMID:9548816]
ChEMBL	The ability to inhibit Toxoplasma gondii Dihydrofolate reductase was tested	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1999) 42: 1007-1017 [PMID:10090784]
ChEMBL	Concentration inhibiting Toxoplasma gondii dihydrofolate reductase.	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1995) 38: 2615-2620 [PMID:7629801]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Toxoplasma gondii (tgDHFR)	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
ChEMBL	Inhibition against Dihydrofolate reductase in Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1997) 40: 1173-1177 [PMID:9089339]
ChEMBL	Inhibition of Toxoplasma gondii Dihydrofolate Reductase	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1995) 38: 1778-1785 [PMID:7752201]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) from Toxoplasma gondii	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1998) 41: 4533-4541 [PMID:9804692]
ChEMBL	Inhibition of Dihydrofolate Reductase of Toxoplasma gondii.	B	5.57	pIC50	2700	nM	IC50	J Med Chem (1997) 40: 479-485 [PMID:9046338]
ChEMBL	Inhibitory activity against recombinant Dihydrofolate reductase from Toxoplasma gondii (tgDHFR)	B	8.14	pIC50	7.2	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
Dihydrofolate reductase in Plasmodium berghei str. ANKA (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3963] [UniProtKB: Q27713]
ChEMBL	In vitro inhibition of dihydrofolate reductase of Plasmodium berghei	B	6.92	pIC50	120	nM	IC50	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) of Plasmodium berghei	B	6.92	pIC50	120	nM	IC50	J Med Chem (1987) 30: 348-356 [PMID:3100802]
ChEMBL	Compound was tested for its inhibition against dihydrofolate reductase enzyme of Plasmodium berghei	B	6.92	pIC50	120	nM	IC50	J Med Chem (1980) 23: 535-541 [PMID:6991695]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) enzyme from Plasmodium berghei	B	6.92	pIC50	120	nM	IC50	J Med Chem (1987) 30: 1998-2004 [PMID:3312605]
ChEMBL	Inhibitory activity against Dihydrofolate reductase enzyme purified from Plasmodium berghei.	B	6.92	pIC50	120	nM	IC50	J Med Chem (1980) 23: 379-384 [PMID:6991692]
Dihydrofolate reductase in Plasmodium falciparum K1 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1939] [UniProtKB: P13922]
ChEMBL	Inhibitory activity against quadruple mutant dihydrofolate reductase (N51I C59R S108N I164L DHFR)	B	5.18	pKi	6664.5	nM	Ki	J Med Chem (2004) 47: 345-354 [PMID:14711307]
ChEMBL	Inhibitory activity against triple mutant dihydrofolate reductase (C59R S108N I164L DHFR)	B	5.24	pKi	5688.7	nM	Ki	J Med Chem (2004) 47: 345-354 [PMID:14711307]
ChEMBL	Inhibition of the C59R+S108N mutant of dihydrofolate reductase (DHFR)	B	6.62	pKi	242.1	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibitory activity against double mutant dihydrofolate reductase (C59R+S108N DHFR)	B	6.62	pKi	242.1	nM	Ki	J Med Chem (2004) 47: 345-354 [PMID:14711307]
ChEMBL	Inhibition of the S108N mutant of dihydrofolate reductase (DHFR)	B	6.88	pKi	132.3	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibitory activity against wild-type dihydrofolate reductase (S108N DHFR)	B	7.99	pKi	10.3	nM	Ki	J Med Chem (2004) 47: 345-354 [PMID:14711307]
ChEMBL	Inhibition of the wild-type dihydrofolate reductase (DHFR)	B	7.99	pKi	10.3	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition of wild type Plasmodium falciparum DHFR using DHF as substrate assessed as inhibition constant in presence of NADPH by UV-Vis spectrometry analysis	B	9.23	pKi	0.59	nM	Ki	RSC Med Chem (2023) 14: 1755-1766 [PMID:37731689]
ChEMBL	Inhibition of Plasmodium falciparum DHFR	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2006) 16: 4366-4370 [PMID:16750361]
dihydrofolate reductase/Dihydrofolate reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374]
ChEMBL	Inhibition of human DHFR	B	4.32	pKi	48000	nM	Ki	Eur J Med Chem (2017) 135: 467-478 [PMID:28477572]
ChEMBL	Inhibition of recombinant human DHFR assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition	B	4.34	pKi	45783.5	nM	Ki	ACS Med Chem Lett (2016) 7: 692-696 [PMID:27437079]
ChEMBL	Inhibition of human DHFR assessed as oxidation of NADPH using dihydrofolate as substrate pre-incubated for 10 mins before substrate addition by spectrophotometry	B	4.72	pKi	19070	nM	Ki	Bioorg Med Chem Lett (2011) 21: 5171-5176 [PMID:21831637]
ChEMBL	Inhibition of human DHFR using dihydrofolate as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric analysis in presence of NADPH	B	4.72	pKi	19070	nM	Ki	J Med Chem (2014) 57: 651-668 [PMID:24428639]
ChEMBL	Inhibition of human DHFR in presence of variable DHFA level by enzyme kinetics assay	B	5.31	pKi	4900	nM	Ki	Bioorg Med Chem (2009) 17: 7324-7336 [PMID:19748785]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	5.86	pKi	1380	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Trypanosoma cruzi.	B	6	pKi	1000	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of human dihydrofolate reductase (DHFR) enzyme	B	6.71	pKi	194.98	nM	Ki	J Med Chem (1995) 38: 967-972 [PMID:7699713]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Leishmania major.	B	6.92	pKi	120	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	8	pKi	10	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of human DHFR assessed as NADPH consumption for conversion of dihydrofolic acid to tetrahydrofolic acid after 6 mins every 5 sec by UV-Visible Spectrophotometer method	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2011) 21: 5428-5431 [PMID:21788136]
ChEMBL	Inhibitory activity against TMP-susceptible Dihydrofolate reductase from Staphylococcus pneumoniae ATCC 49619	B	4.47	pIC50	34000	nM	IC50	J Med Chem (2003) 46: 2304-2312 [PMID:12773035]
ChEMBL	Inhibition of human recombinant DHFR	B	4.49	pIC50	32200	nM	IC50	J Med Chem (2013) 56: 4422-4441 [PMID:23627352]
ChEMBL	Inhibitory concentration against dihydrofolate reductace enzyme of Cryptosporidium parvum of human origin	B	5.3	pIC50	5000	nM	IC50	J Med Chem (2004) 47: 1475-1486 [PMID:14998335]
ChEMBL	Compound was evaluated as inhibitor of human Dihydrofolate reductase	B	5.47	pIC50	3400	nM	IC50	J Med Chem (2003) 46: 591-600 [PMID:12570380]
ChEMBL	Antibacterial activity against TMP-susceptible Dihydrofolate reductase from Staphylococcus pneumoniae ATCC 49619	B	5.52	pIC50	3000	nM	IC50	J Med Chem (2003) 46: 2304-2312 [PMID:12773035]
ChEMBL	Inhibitory concentration against dihydrofolate reductace enzyme of Cryptosporidium parvum of bovine origin	B	5.7	pIC50	2000	nM	IC50	J Med Chem (2004) 47: 1475-1486 [PMID:14998335]
ChEMBL	Concentration required to inhibit the human Dihydrofolate reductase by 50% was determined (reported by workers at Hoffman-LaRoche)	B	6.05	pIC50	900	nM	IC50	J Med Chem (2004) 47: 2475-2485 [PMID:15115391]
ChEMBL	In vitro inhibition of dihydrofolate reductase of Neisseria gonorrhoeae	B	6.35	pIC50	450	nM	IC50	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
ChEMBL	Inhibitory activity against Dihydrofolate reductase in Neisseria gonorrhoeae	B	6.35	pIC50	450	nM	IC50	J Med Chem (1989) 32: 1936-1942 [PMID:2502633]
ChEMBL	Inhibitory activity against dihydrofolate reductase (DHFR) enzyme from Neisseria gonorrhoeae	B	6.35	pIC50	450	nM	IC50	J Med Chem (1987) 30: 1998-2004 [PMID:3312605]
ChEMBL	Inhibition of Neisseria gonorrhoeae Dihydrofolate reductase(DHFR)	B	6.35	pIC50	450	nM	IC50	J Med Chem (1988) 31: 122-129 [PMID:3121854]
ChEMBL	Inhibition of DHFR using dihydrofolate as substrate following 3 mins substrate preincubation measured after 3 mins by spectrophotometric analysis	B	6.91	pIC50	123	nM	IC50	Bioorg Med Chem Lett (2012) 22: 2428-2433 [PMID:22406152]
ChEMBL	Inhibitory activity against TMP-susceptible DHFR from Staphylococcus pneumoniae ATCC 49619	B	7.12	pIC50	75	nM	IC50	J Med Chem (2003) 46: 2304-2312 [PMID:12773035]
ChEMBL	Inhibition of Streptococcus pneumoniae dihydrofolate reductase (DHFR) enzyme by Iclaprim	B	7.12	pIC50	75	nM	IC50	Bioorg Med Chem Lett (2003) 13: 4217-4221 [PMID:14623005]
ChEMBL	Inhibitory activity against Dihydrofolate reductase from recombinant human (hDHFR)	B	7.27	pIC50	54	nM	IC50	J Med Chem (1998) 41: 3426-3434 [PMID:9719595]
dihydrofolate reductase/Dihydrofolate reductase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4564] [GtoPdb: 2603] [UniProtKB: P00375]
ChEMBL	Competitive inhibition of mouse DHFR by enzyme kinetics assay in presence of variable DHFA level	B	6.3	pKi	500	nM	Ki	Bioorg Med Chem (2009) 17: 7324-7336 [PMID:19748785]
dihydrofolate reductase/Dihydrofolate reductase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2]
ChEMBL	Inhibition of rat liver DHFR by enzyme kinetics assay in presence of variable DHFA level	B	4.84	pKi	14300	nM	Ki	Bioorg Med Chem (2009) 17: 7324-7336 [PMID:19748785]
ChEMBL	Inhibition of rat liver DHFR	B	0.74	pIC50	180000000	nM	IC50	J Med Chem (2007) 50: 3046-3053 [PMID:17552508]
ChEMBL	Inhibitory concentration against rat liver (RL) dihydrofolate reductases (DHFR)	B	4.4	pIC50	40000	nM	IC50	J Med Chem (1993) 36: 3437-3443 [PMID:8230134]
ChEMBL	Inhibition of Rat liver Dihydrofolate reductase(DHFR)	B	4.47	pIC50	34000	nM	IC50	J Med Chem (1988) 31: 122-129 [PMID:3121854]
Dihydrofolate reductase in Pneumocystis jirovecii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2057] [UniProtKB: Q9UUP5]
ChEMBL	Inhibition of Pneumocystis jirovecii recombinant DHFR expressed in Escherichia coli Rosetta Gami B (DE3) competent cells using DHFA as substrate and NADPH	B	7.04	pIC50	92	nM	IC50	Bioorg Med Chem (2018) 26: 2640-2650 [PMID:29691153]
Dihydrofolate reductase in Salmonella enterica subsp. enterica serovar Typhi (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4888] [UniProtKB: Q8Z9J9]
ChEMBL	Inhibitory activity against Escherichia coli dihydrofolate reductase	B	8.08	pKi	8.32	nM	Ki	J Med Chem (1991) 34: 46-54 [PMID:1899453]
ChEMBL	Inhibitory activity against Escherichia coli dihydrofolate reductase	B	8.08	pKi	8.32	nM	Ki	J Med Chem (1991) 34: 46-54 [PMID:1899453]
Dihydrofolate reductase in Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4296314] [UniProtKB: Q54801]
ChEMBL	Inhibition of trimethoprim-resistant Streptococcus pneumoniae DHFR I100L mutant	B	4.38	pIC50	42000	nM	IC50	J Med Chem (2007) 50: 4374-4381 [PMID:17696333]
Dihydrofolate reductase in Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcuslactis) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4012] [UniProtKB: Q59487]
ChEMBL	In vitro inhibition of dihydrofolate reductase enzymes in Neisseria gonorrhoeae	B	6.35	pIC50	450	nM	IC50	J Med Chem (1989) 32: 1949-1958 [PMID:2754716]
Dihydrofolate reductase in Saccharomyces cerevisiae S288c (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2576] [UniProtKB: P07807]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase.	B	4.92	pIC50	12000	nM	IC50	J Med Chem (1996) 39: 1836-1845 [PMID:8627607]
Dihydrofolate reductase type 1 in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2627] [UniProtKB: P00382]
ChEMBL	Inhibitory activity to dihydrofolate reductase in Escherichia coli	B	6.3	pIC50	500	nM	IC50	J Med Chem (1989) 32: 1927-1935 [PMID:2502632]
NH(3)-dependent NAD(+) synthetase in Bacillus subtilis (strain 168) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4615] [UniProtKB: P08164]
ChEMBL	Inhibitory activity against Bacillus subtilis NAD synthetase	B	6	pIC50	>1000	nM	IC50	J Med Chem (2003) 46: 3371-3381 [PMID:12852767]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JB8 after 72 hrs by SYBR green assay	F	4.15	pIC50	70393	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum WE2 after 72 hrs by SYBR green assay	F	4.21	pIC50	61785	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum DAN after 72 hrs by SYBR green assay	F	4.22	pIC50	59746	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum KC2 after 72 hrs by SYBR green assay	F	4.3	pIC50	50695	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum AL2 after 72 hrs by SYBR green assay	F	4.31	pIC50	48456	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum DEV after 72 hrs by SYBR green assay	F	4.32	pIC50	48211	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JF6 after 72 hrs by SYBR green assay	F	4.32	pIC50	48189	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum KC5 after 72 hrs by SYBR green assay	F	4.35	pIC50	44582	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum XB3 after 72 hrs by SYBR green assay	F	4.36	pIC50	43831	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum KB8 after 72 hrs by SYBR green assay	F	4.38	pIC50	41742	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JB12 after 72 hrs by SYBR green assay	F	4.4	pIC50	40053	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum W2 harboring polymorphic DHFR after 72 hrs by SYBR green assay	F	4.4	pIC50	40000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum XD8 after 72 hrs by SYBR green assay	F	4.4	pIC50	39806	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JC9 after 72 hrs by SYBR green assay	F	4.4	pIC50	39642	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum NF10 after 72 hrs by SYBR green assay	F	4.4	pIC50	39605	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay	F	4.44	pIC50	36531	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antimalarial activity against Plasmodium falciparum Kenyan isolates expressing DHFR Ser108Asn, Asn51Ile and Cys59Arg triple mutant by [3H]hypoxanthine incorporation assay	F	4.47	pIC50	33655	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3793-3798 [PMID:19528269]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum WC4 after 72 hrs by SYBR green assay	F	4.49	pIC50	32669	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum D10 harboring polymorphic DHFR after 72 hrs by SYBR green assay	F	4.52	pIC50	30000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum D2 after 72 hrs by SYBR green assay	F	4.53	pIC50	29852	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antimalarial activity against Plasmodium falciparum Kenyan isolates by [3H]hypoxanthine incorporation assay	F	4.53	pIC50	29656	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3793-3798 [PMID:19528269]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum LC12 after 72 hrs by SYBR green assay	F	4.62	pIC50	24165	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum HB3 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antimalarial activity against Plasmodium falciparum Kenyan isolates expressing DHFR Ser108Asn and Cys59Arg or Ser108Asn and Asn51Ile double mutant by [3H]hypoxanthine incorporation assay	F	5.03	pIC50	9308.6	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3793-3798 [PMID:19528269]
ChEMBL	In vitro antiplasmodial activity (IC50) against Plasmodium falciparum wtTM4/8.2	F	5.17	pIC50	6700	nM	IC50	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Antiplasmodial activity against PYR-sensitive Plasmodium falciparum TM4 assessed as inhibition of parasite growth by [3H]-hypoxanthine incorporation assay	F	5.17	pIC50	6700	nM	IC50	RSC Med Chem (2023) 14: 1755-1766 [PMID:37731689]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum GB4 harboring polymorphic DHFR after 72 hrs by SYBR green assay	F	5.39	pIC50	4100	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum XG10 after 72 hrs by SYBR green assay	F	5.48	pIC50	3311	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum D10 after 72 hrs by SYBR green assay	F	5.5	pIC50	3162.28	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 harboring polymorphic DHFR after 72 hrs by SYBR green assay	F	5.55	pIC50	2800	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum WF12 after 72 hrs by SYBR green assay	F	5.58	pIC50	2657	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against transgenic Plasmodium falciparum Dd2 pDT harboring polymorphic DHFR after 72 hrs by SYBR green assay	F	5.59	pIC50	2600	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum AUD after 72 hrs by SYBR green assay	F	5.61	pIC50	2481	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum NIC after 72 hrs by SYBR green assay	F	5.64	pIC50	2303	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum XF12 after 72 hrs by SYBR green assay	F	5.64	pIC50	2273	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum QF5 after 72 hrs by SYBR green assay	F	5.67	pIC50	2155	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum KA6 after 72 hrs by SYBR green assay	F	5.68	pIC50	2108	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JON after 72 hrs by SYBR green assay	F	5.68	pIC50	2071	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JC3 after 72 hrs by SYBR green assay	F	5.69	pIC50	2032	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JF11 after 72 hrs by SYBR green assay	F	5.79	pIC50	1640	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum JH6 after 72 hrs by SYBR green assay	F	5.82	pIC50	1503	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum KH7 after 72 hrs by SYBR green assay	F	5.85	pIC50	1412	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay	F	5.9	pIC50	1258.93	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay	F	5.9	pIC50	1258.93	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum LA10 after 72 hrs by SYBR green assay	F	5.93	pIC50	1183	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum TF1 after 72 hrs by SYBR green assay	F	5.96	pIC50	1096	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum XE7 after 72 hrs by SYBR green assay	F	6.07	pIC50	855	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antimalarial activity against pyrimethamine-sensitive Plasmodium falciparum 3D7 expressing wild type DHFR by [3H]hypoxanthine incorporation assay	F	6.39	pIC50	406.7	nM	IC50	Antimicrob Agents Chemother (2009) 53: 3793-3798 [PMID:19528269]
ChEMBL	Antimalarial activity against Plasmodium falciparum FCR3 infected in human erythrocytes by Giemsa staining analysis	F	6.42	pIC50	380.19	nM	IC50	Bioorg Med Chem (2019) 27: 3574-3586 [PMID:31272837]
Organic cation transporter 1/Solute carrier family 22 member 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5685] [GtoPdb: 1019] [UniProtKB: O15245]
ChEMBL	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	B	4.25	pIC50	56800	nM	IC50	J Med Chem (2008) 51: 5932-5942 [PMID:18788725]
ChEMBL	Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay	B	4.3	pIC50	50680	nM	IC50	J Med Chem (2017) 60: 2685-2696 [PMID:28230985]
TAAR5/Trace amine-associated receptor 5 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3784908] [GtoPdb: 170] [UniProtKB: Q5QD14]
ChEMBL	Agonist activity at mouse TAAR5 expressed in HEK293 cells assessed as cAMP accumulation after 20 mins by BRET assay	F	5	pEC50	>10000	nM	EC50	Eur J Med Chem (2017) 127: 781-792 [PMID:27823885]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]