ChEMBL ligand: CHEMBL12980
Muscarinic acetylcholine receptor DM1 in Drosophila melanogaster [ChEMBL: CHEMBL2366467] [UniProtKB: P16395] There should be some charts here, you may need to enable JavaScript!
M1 receptor/Muscarinic acetylcholine receptor M1 in Human [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229] Muscarinic acetylcholine receptor M1 in Bovine [ChEMBL: CHEMBL2672] [UniProtKB: Q8WMX0] There should be some charts here, you may need to enable JavaScript!
M2 receptor/Muscarinic acetylcholine receptor M2 in Human [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172] M2 receptor/Muscarinic acetylcholine receptor M2 in Rat [ChEMBL: CHEMBL309] [GtoPdb: 14] [UniProtKB: P10980] There should be some charts here, you may need to enable JavaScript!
Muscarinic acetylcholine receptor M3 in Guinea pig [ChEMBL: CHEMBL5498] [UniProtKB: Q8VH26] There should be some charts here, you may need to enable JavaScript!
Vesicular acetylcholine transporter in Rat [ChEMBL: CHEMBL2125] [GtoPdb: 1013] [UniProtKB: Q62666] There should be some charts here, you may need to enable JavaScript!
M4 receptor in Human [GtoPdb: 16] [UniProtKB: P08173] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Muscarinic acetylcholine receptor DM1 in Drosophila melanogaster (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366467] [UniProtKB: P16395]
ChEMBL	Displacement of [3H]QNB from Drosophila melanogaster mAChR by scintillation counting	B	9.3	pKi	0.5	nM	Ki	J Agric Food Chem (2007) 55: 2276-2281 [PMID:17319687]
ChEMBL	Displacement of [3H]AF-DX 384 from Drosophila melanogaster mAChR by scintillation counting	B	9.3	pKi	0.5	nM	Ki	J Agric Food Chem (2007) 55: 2276-2281 [PMID:17319687]
M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
ChEMBL	Binding affinity for muscarinic acetylcholine receptor M1 by measuring displacement of [3H]QNB from guinea pig cerebral cortex	B	10.29	pKi	0.05	nM	Ki	J Med Chem (1995) 38: 473-487 [PMID:7853341]
Muscarinic acetylcholine receptor M1 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2672] [UniProtKB: Q8WMX0]
ChEMBL	In vitro binding affinity against bovine striatal membrane using [3H]pirenzepine	B	9.66	pKi	0.22	nM	Ki	J Med Chem (1995) 38: 1711-1719 [PMID:7752195]
M2 receptor/Muscarinic acetylcholine receptor M2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172]
ChEMBL	Binding affinity for muscarinic acetylcholine receptor M2 by measuring displacement of [3H]QNB from guinea pig heart	B	10.35	pKi	0.04	nM	Ki	J Med Chem (1995) 38: 473-487 [PMID:7853341]
M2 receptor/Muscarinic acetylcholine receptor M2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL309] [GtoPdb: 14] [UniProtKB: P10980]
ChEMBL	In vitro binding affinity against rat heart membrane using [3H]-AF-DX-384	B	9.74	pKi	0.18	nM	Ki	J Med Chem (1995) 38: 1711-1719 [PMID:7752195]
Muscarinic acetylcholine receptor M3 in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5498] [UniProtKB: Q8VH26]
ChEMBL	In vitro binding affinity against guinea pig ileum using [3H]N-methylscopolamine	B	8.33	pKi	4.63	nM	Ki	J Med Chem (1995) 38: 1711-1719 [PMID:7752195]
ChEMBL	Binding affinity for muscarinic acetylcholine receptor M3 by measuring displacement of [3H]QNB from guinea pig parotid gland	B	9.62	pKi	0.24	nM	Ki	J Med Chem (1995) 38: 473-487 [PMID:7853341]
Vesicular acetylcholine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2125] [GtoPdb: 1013] [UniProtKB: Q62666]
ChEMBL	Displacement of [125I]O-iodo-trans-decalinvesamicol from VAChT in Sprague-Dawley rat cerebral membrane after 1 hr by gamma counting	B	6.24	pKi	581.7	nM	Ki	Bioorg Med Chem (2012) 20: 4936-4941 [PMID:22831799]
M4 receptor in Human [GtoPdb: 16] [UniProtKB: P08173]
GtoPdb	-	-	10.4	pKi	0.04	nM	Ki	Invest Ophthalmol Vis Sci (2018) 59: 2778-2791 [PMID:29860464]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]