ChEMBL ligand: CHEMBL76688 (LY-544349, LY544349, ONO-5046, Sivelestat)
cathepsin G/Cathepsin G in Human [ChEMBL: CHEMBL4071] [GtoPdb: 2348] [UniProtKB: P08311] There should be some charts here, you may need to enable JavaScript!
kallikrein 1/Kallikrein 1 in Human [ChEMBL: CHEMBL2319] [GtoPdb: 2865] [UniProtKB: P06870] There should be some charts here, you may need to enable JavaScript!
elastase, neutrophil expressed/Leukocyte elastase in Human [ChEMBL: CHEMBL248] [GtoPdb: 2358] [UniProtKB: P08246] There should be some charts here, you may need to enable JavaScript!
proteinase 3/Leukocyte proteinase 3 in Human [ChEMBL: CHEMBL3900] [GtoPdb: 2401] [UniProtKB: P24158] There should be some charts here, you may need to enable JavaScript!
elastase, neutrophil expressed in Human [GtoPdb: 2358] [UniProtKB: P08246] elastase, neutrophil expressed/Neutrophil elastase in Mouse [ChEMBL: CHEMBL5156] [GtoPdb: 2358] [UniProtKB: Q3UP87] There should be some charts here, you may need to enable JavaScript!
coagulation factor II, thrombin/Thrombin in Human [ChEMBL: CHEMBL204] [GtoPdb: 2362] [UniProtKB: P00734] Thrombin in Bovine [ChEMBL: CHEMBL4471] [UniProtKB: P00735] There should be some charts here, you may need to enable JavaScript!
plasminogen activator, urokinase/Urokinase-type plasminogen activator in Human [ChEMBL: CHEMBL3286] [GtoPdb: 2393] [UniProtKB: P00749] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
cathepsin G/Cathepsin G in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4071] [GtoPdb: 2348] [UniProtKB: P08311]
ChEMBL	Inhibition of human cathepsin G using Suc-Ala-Ala-Pro-Phe-p-nitroanilide as substrate incubated for 30 mins prior to substrate addition measured for 30 mins by spectrophotometry	B	4	pIC50	>100000	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]
ChEMBL	Inhibition of human cathepsin G using N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as substrate measured for 180 mins by spectrophotometry	B	5	pIC50	>10000	nM	IC50	Bioorg Med Chem (2015) 23: 1123-1134 [PMID:25650311]
kallikrein 1/Kallikrein 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2319] [GtoPdb: 2865] [UniProtKB: P06870]
ChEMBL	Inhibition of human kallikrein using H-Pro-Phe-Arg-AMC acetate salt as substrate measured for 30 mins by spectrophotometry	B	5.7	pIC50	>2000	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]
elastase, neutrophil expressed/Leukocyte elastase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL248] [GtoPdb: 2358] [UniProtKB: P08246]
ChEMBL	Binding affinity against Elastase.	B	6.7	pKi	200	nM	Ki	J Med Chem (2000) 43: 305-341 [PMID:10669559]
ChEMBL	Inhibition of human neutrophil elastase	B	6.7	pKi	200	nM	Ki	J Med Chem (2020) 63: 13258-13265 [PMID:32692176]
ChEMBL	Inhibition of human leukocyte elastase	B	7.43	pKi	37	nM	Ki	ACS Med Chem Lett (2018) 9: 345-350 [PMID:29670698]
ChEMBL	Inhibition of human neutrophil elastase	B	7.62	pKi	24	nM	Ki	Bioorg Med Chem Lett (2021) 52: 128380-128380 [PMID:34563669]
ChEMBL	Inhibition Assay: Each of Compounds (1) to (7) was added into 50 μL buffer A (containing 20 mM Tris-HCl (pH 7.4), 0.1% NaN3 and 5 mM CaCl2) to obtain compound solutions with concentrations of 0.02, 0.2, 2, or 20 μM for each of Compounds (1) to (7), followed by m adding 50 μL of the compound solutions into wells of a 96-well plate. The buffer A and Sivelestat (under the same solvent condition and concentrations as those of Compounds (1) to (7)) with the same volume were respectively used as a control group and a positive control group. Each well of the 96-well plate was further added with 25 μL of human neutrophil elastase (Enzo, 200 nM, in buffer B containing 20 mM Tris-HCl (pH 7.4) and 0.1% NaN3) and 25 μL MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide (500 μM, prepared in the buffer B), followed by reacting in an incubator (30° C.) for 30 minutes.	B	4.19	pIC50	65040	nM	IC50	US-9073833-B1. Oxime-based compound, pharmaceutical composition containing the same and method for preparing the same (2015)
ChEMBL	Inhibition of human neutrophil elastase incubated for 40 mins in presence of Boc-Gln-Ala-Arg-AMC by fluorescence microtiter plate method	B	6.15	pIC50	704	nM	IC50	J Med Chem (2022) 65: 2971-2987 [PMID:35005973]
ChEMBL	Inhibition of human neutrophil elastase assessed as proteolysis using N-(methoxysuccinyl)-Ala-Ala-Pro-Val-p-nitroanilide substrate incubated for 15 mins prior to substrate addition measured every 30 seconds	B	6.87	pIC50	136	nM	IC50	J Med Chem (2013) 56: 1276-1290 [PMID:23350733]
ChEMBL	Inhibition of elastase in human neutrophil using N-(OMe-succinyl)-Ala-Ala-Pro-Val-p-nitroanilide as substrate preincubated with enzyme for 15 mins followed by substrate addition and measured every 30s for 30 mins by absorbance based analysis	B	6.89	pIC50	128	nM	IC50	Bioorg Med Chem (2020) 28: 115756-115756 [PMID:33002682]
ChEMBL	Inhibition of human neutrophil elastase measured for 30 mins by spectrophotometry	B	7.15	pIC50	70	nM	IC50	Bioorg Med Chem (2015) 23: 1123-1134 [PMID:25650311]
ChEMBL	Release Assay: Each of Compounds (1) to (6) was dissolved in 100% DMSO so as to obtain stock to compound solutions with concentrations of 0.01, 0.03, 0.1, 0.3, 1, 3 or 10 mM) for each of Compounds (1) to (6). 7500 of the neutrophil suspension obtained in -3. Preparation of human neutrophil under the section of -Experimental materials was added with 200 uM of MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide (454454, Calbiochem Limited, dissolved in 750 ul HBSS and functioned as a substrate for human neutrophils) at a is volume ratio of 1:1 (the final volume was 1.5 ml), stirred at 37 C. for 2 minutes, followed by addition with 1.5 ul of the aforesaid compound solution and reaction at 37 C. for 2 minutes. In a control group, 100% DMSO was used to substitute the compound solution. Moreover, Sivelestat (with concentrations of 0.01, 0.03, 0.1, 0.3, 1, 3 or 10 mM in DMSO) was used to substitute the compound solution as a positive control group.	B	7.3	pIC50	50	nM	IC50	US-9073833-B1. Oxime-based compound, pharmaceutical composition containing the same and method for preparing the same (2015)
ChEMBL	Inhibition of human neutrophil elastase using N-methylsuccinyl-Ala-Ala-Pro-Val-7-amino-4-methylcoumarin as substrate measured for every 30 sec for 10 mins by fluorescence based assay	B	7.3	pIC50	50	nM	IC50	Bioorg Med Chem (2021) 29: 115836-115836 [PMID:33218895]
ChEMBL	Inhibition of human neutrophil elastase	B	7.36	pIC50	44	nM	IC50	J Med Chem (2020) 63: 13258-13265 [PMID:32692176]
ChEMBL	Inhibitory concentration in vitro against Human neutrophil elastase	B	7.36	pIC50	44	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2349-2353 [PMID:12161131]
ChEMBL	Inhibition of human neutrophil elastase using N-methylsuccinyl-Ala-Ala-Pro-Val-7-amino-4-methyl-coumarin as substrate measured every 30 secs for 10 mins by fluorescence microplate reader	B	7.36	pIC50	44	nM	IC50	Bioorg Med Chem (2011) 19: 4460-4472 [PMID:21741848]
ChEMBL	Inhibitory concentration against elastase	B	7.36	pIC50	44	nM	IC50	J Med Chem (2000) 43: 305-341 [PMID:10669559]
GtoPdb	-	-	7.4	pIC50	44	nM	IC50	Bioorg Med Chem (2011) 19: 4460-72 [PMID:21741848]
ChEMBL	Inhibition of human neutrophil elastase using MeO-Suc-Ala-Ala-Pro-Val-AMC as substrate incubated for 30 mins prior to substrate addition measured for 30 mins by fluorescence assay	B	8	pIC50	10	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]
ChEMBL	Inhibition of elastase in human neutrophil using MeOSuc-AAPV-AMC as substrate by fluorescence based analysis	B	8.46	pIC50	3.5	nM	IC50	Bioorg Med Chem (2020) 28: 115756-115756 [PMID:33002682]
proteinase 3/Leukocyte proteinase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3900] [GtoPdb: 2401] [UniProtKB: P24158]
ChEMBL	Inhibition of human proteinase 3 using N-MeOSuc-Ala-Ala-Pro-Val-p-nitroanilide as substrate measured for 30 mins by spectrophotometry	B	4	pIC50	>100000	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]
ChEMBL	Inhibition of Pr3 (unknown origin) using t-butyloxycarbonyl-Ala-Ala-Nva-thiobenzyl ester as substrate measured for 180 mins by spectrophotometry	B	6.47	pIC50	340	nM	IC50	Bioorg Med Chem (2015) 23: 1123-1134 [PMID:25650311]
elastase, neutrophil expressed in Human [GtoPdb: 2358] [UniProtKB: P08246]
GtoPdb	-	-	7.4	pIC50	44	nM	IC50	Bioorg Med Chem (2011) 19: 4460-72 [PMID:21741848]
elastase, neutrophil expressed/Neutrophil elastase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5156] [GtoPdb: 2358] [UniProtKB: Q3UP87]
ChEMBL	Inhibition of recombinant mouse neutrophil elastase using methoxysuccinyl-Ala-Ala-Pro-Val-pnitroanilide as substrate preincubated for 15 mins before substrate addition measured after 90 mins by spectrophotometry	B	6.84	pIC50	145.39	nM	IC50	Bioorg Med Chem (2015) 23: 1123-1134 [PMID:25650311]
coagulation factor II, thrombin/Thrombin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL204] [GtoPdb: 2362] [UniProtKB: P00734]
ChEMBL	Inhibition of human thrombin using Z-Gly-Gly-Arg-AMC.HCl as substrate measured for 30 mins by spectrophotometry	B	5.7	pIC50	>2000	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]
Thrombin in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4471] [UniProtKB: P00735]
ChEMBL	Inhibition of bovine plasma thrombin using Benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin hydrochloride by spectrophotometry	B	5	pIC50	>10000	nM	IC50	Bioorg Med Chem (2015) 23: 1123-1134 [PMID:25650311]
plasminogen activator, urokinase/Urokinase-type plasminogen activator in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3286] [GtoPdb: 2393] [UniProtKB: P00749]
ChEMBL	Inhibition of human urokinase using Z-Gly-Gly-Arg-AMC.HCl as substrate measured for 30 mins by spectrophotometry	B	5.7	pIC50	>2000	nM	IC50	J Med Chem (2013) 56: 9802-9806 [PMID:24224573]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]