ChEMBL ligand: CHEMBL9298 (CGS-16949A, Fadrozole)
CYP11B1/Cytochrome P450 11B1 in Human [ChEMBL: CHEMBL1908] [GtoPdb: 1359] [UniProtKB: P15538] There should be some charts here, you may need to enable JavaScript!
CYP11B2/Cytochrome P450 11B2 in Human [ChEMBL: CHEMBL2722] [GtoPdb: 1360] [UniProtKB: P19099] CYP11B2/Cytochrome P450 11B2 in Rat [ChEMBL: CHEMBL3237] [GtoPdb: 1360] [UniProtKB: P30099] There should be some charts here, you may need to enable JavaScript!
CYP11B2 in Human [GtoPdb: 1360] [UniProtKB: P19099] CYP11B2/Cytochrome P450 11B2, mitochondrial in Mouse [ChEMBL: CHEMBL3621020] [GtoPdb: 1360] [UniProtKB: P15539] There should be some charts here, you may need to enable JavaScript!
CYP17A1/Cytochrome P450 17A1 in Human [ChEMBL: CHEMBL3522] [GtoPdb: 1361] [UniProtKB: P05093] There should be some charts here, you may need to enable JavaScript!
CYP19A1/Cytochrome P450 19A1 in Human [ChEMBL: CHEMBL1978] [GtoPdb: 1362] [UniProtKB: P11511] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
CYP11B1/Cytochrome P450 11B1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1908] [GtoPdb: 1359] [UniProtKB: P15538]
GtoPdb	-	-	8.66	pKi	2.2	nM	Ki	Anal Biochem (2009) 394: 56-61 [PMID:19622340]
ChEMBL	In vitro inhibition of ACTH-stimulated corticosterone biosynthesis in rat adrenal slices	F	4.1	pIC50	80000	nM	IC50	J Med Chem (1995) 38: 2103-2111 [PMID:7783141]
ChEMBL	In Vitro Inhibition Assay: A suspension of fission yeast (S. pombe PE1) with a cell density of 3˙10^7 cells/ml was prepared on a freshly grown culture using fresh EMMG (pH 7.4) as modified according to Ehmer et al. (Ehmer, P. B. et al., 1. Steroid. Biochem. Mol. Biol. 81, 173-179 (2002)). 492.5 μl of this cell suspension was admixed with 5 μl of inhibitor solution (50 μM of the compound to be tested in ethanol or DMSO) and incubated at 32 °C. for 15 min. Controls were admixed with 5 μl of ethanol. The enzyme reaction was started by adding 2.5 μl of 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in Ethanol), followed by horizontal shaking at 32 °C. for 6 h. The test was quenched by extracting the sample with 500 μl of EtOAc. After centrifugation (10,000 g, 2 min), the EtOAc phase was removed and evaporated to dryness.	B	7.55	pIC50	28.3	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibitory concentration against human cytochrome P450 11B1 expressed in fission yeast, incubated with [14C]deoxycorticosterone	B	8	pIC50	10	nM	IC50	J Med Chem (2005) 48: 6632-6642 [PMID:16220979]
ChEMBL	Inhibition of human CYP11B1 expressed in V79 11B1 cells	B	8	pIC50	10	nM	IC50	J Med Chem (2006) 49: 2222-2231 [PMID:16570918]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79 MZh cells	B	8	pIC50	10	nM	IC50	J Med Chem (2008) 51: 5064-5074 [PMID:18672861]
ChEMBL	Inhibition of human adrenal corticoid CYP11B1 expressed in chinese hamster V79 MZh cells	B	8	pIC50	10	nM	IC50	J Med Chem (2008) 51: 6138-6149 [PMID:18763754]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79 MZh cells	B	8	pIC50	10	nM	IC50	J Med Chem (2008) 51: 8077-8087 [PMID:19049427]
ChEMBL	Inhibition of human CYP11B1 expressed in Chinese hamster V79MZ cells using [1,2-3H]11-deoxycorticosterone/11-deoxycorticosterone	B	8	pIC50	10	nM	IC50	J Med Chem (2011) 54: 1613-1625 [PMID:21341743]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79 MZh cells assessed as conversion of [4-14C]-11-deoxycorticosterone substrate by HPTLC assay	B	8	pIC50	10	nM	IC50	J Med Chem (2011) 54: 2307-2319 [PMID:21384875]
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	8	pIC50	10	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	In vitro inhibitory concentration against human CYP11B1 expressed in V79 MZh hamster fibroblasts incubated with 100 nM of substrate deoxy-corticosterone in presence of the compound	B	8	pIC50	10	nM	IC50	J Med Chem (2005) 48: 1563-1575 [PMID:15743198]
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	8	pIC50	10	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	8.01	pIC50	9.7	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	In vitro inhibition of [14C]deoxycorticosterone binding to human cytochrome P450 11B1 expressed in hamster V79 MZh cells	B	8.01	pIC50	9.7	nM	IC50	J Med Chem (2005) 48: 1796-1805 [PMID:15771425]
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	8.05	pIC50	9	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZ cells using [3H]-11-deoxycorticosterone as substrate after 1 hr by HPLC analysis	B	8.2	pIC50	6.3	nM	IC50	Eur J Med Chem (2015) 89: 597-605 [PMID:25462268]
ChEMBL	Inhibition of CYP11B1 in human V79MZ cells using [3H]-11-deoxycorticosterone as substrate incubated for 1 hr prior to substrate addition measured after 25 mins by HPLC analysis	B	8.2	pIC50	6.3	nM	IC50	Eur J Med Chem (2015) 90: 788-796 [PMID:25528333]
ChEMBL	Inhibition of human CYP11B1 expressed in V79 MZh cells using [14C]-deoxycorticosterone substrate incubated for 6 hrs by HPTLC method	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2015) 58: 2530-2537 [PMID:25711516]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using deoxycorticosterone as substrate	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2014) 57: 5179-5189 [PMID:24899257]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2013) 56: 6101-6107 [PMID:23859149]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2013) 56: 1723-1729 [PMID:23363058]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxy-corticosterone as substrate	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
ChEMBL	Inhibition of human CYP11B1 expressed in V79MZh cells using [14C]-11-deoxycorticosterone as substrate by HPTLC/phosphoimaging method	B	8.2	pIC50	6.3	nM	IC50	J Med Chem (2012) 55: 7080-7089 [PMID:22861193]
CYP11B2/Cytochrome P450 11B2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2722] [GtoPdb: 1360] [UniProtKB: P19099]
GtoPdb	-	-	9.1	pKi	0.8	nM	Ki	Anal Biochem (2009) 394: 56-61 [PMID:19622340]
ChEMBL	In Vitro Inhibition Assay: A suspension of fission yeast (S. pombe PE1) with a cell density of 3˙10^7 cells/ml was prepared on a freshly grown culture using fresh EMMG (pH 7.4) as modified according to Ehmer et al. (Ehmer, P. B. et al., 1. Steroid. Biochem. Mol. Biol. 81, 173-179 (2002)). 492.5 μl of this cell suspension was admixed with 5 μl of inhibitor solution (50 μM of the compound to be tested in ethanol or DMSO) and incubated at 32 °C. for 15 min. Controls were admixed with 5 μl of ethanol. The enzyme reaction was started by adding 2.5 μl of 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in Ethanol), followed by horizontal shaking at 32 °C. for 6 h. The test was quenched by extracting the sample with 500 μl of EtOAc. After centrifugation (10,000 g, 2 min), the EtOAc phase was removed and evaporated to dryness.	B	4.68	pIC50	20870	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	In Vitro Inhibition Assay: A suspension of fission yeast (S. pombe PE1) with a cell density of 3˙10^7 cells/ml was prepared on a freshly grown culture using fresh EMMG (pH 7.4) as modified according to Ehmer et al. (Ehmer, P. B. et al., 1. Steroid. Biochem. Mol. Biol. 81, 173-179 (2002)). 492.5 μl of this cell suspension was admixed with 5 μl of inhibitor solution (50 μM of the compound to be tested in ethanol or DMSO) and incubated at 32 °C. for 15 min. Controls were admixed with 5 μl of ethanol. The enzyme reaction was started by adding 2.5 μl of 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in Ethanol), followed by horizontal shaking at 32 °C. for 6 h. The test was quenched by extracting the sample with 500 μl of EtOAc. After centrifugation (10,000 g, 2 min), the EtOAc phase was removed and evaporated to dryness.	B	7.73	pIC50	18.7	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition of human CYP11B2 expressed in renal leiomyoblastoma cells	B	8.22	pIC50	6	nM	IC50	J Med Chem (2015) 58: 8054-8065 [PMID:26403853]
ChEMBL	Inhibition of human CYP11B2 expressed in V79 11B2 cells	B	9	pIC50	1	nM	IC50	J Med Chem (2006) 49: 2222-2231 [PMID:16570918]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79 MZh cells	B	9	pIC50	1	nM	IC50	J Med Chem (2008) 51: 5064-5074 [PMID:18672861]
ChEMBL	Inhibition of human adrenal corticoid CYP11B2 expressed in chinese hamster V79 MZh cells	B	9	pIC50	1	nM	IC50	J Med Chem (2008) 51: 6138-6149 [PMID:18763754]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79 MZh cells	B	9	pIC50	1	nM	IC50	J Med Chem (2008) 51: 8077-8087 [PMID:19049427]
ChEMBL	Inhibition of human CYP11B2 expressed in Chinese hamster V79MZ cells using [1,2-3H]11-deoxycorticosterone/11-deoxycorticosterone	B	9	pIC50	1	nM	IC50	J Med Chem (2011) 54: 1613-1625 [PMID:21341743]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79 MZh cells assessed as conversion of [4-14C]-11-deoxycorticosterone substrate by HPTLC assay	B	9	pIC50	1	nM	IC50	J Med Chem (2011) 54: 2307-2319 [PMID:21384875]
ChEMBL	In vitro inhibition of [14C]deoxycorticosterone binding to human cytochrome P450 11B2 expressed in hamster V79 MZh cells	B	9	pIC50	1	nM	IC50	J Med Chem (2005) 48: 1796-1805 [PMID:15771425]
ChEMBL	In vitro inhibitory concentration against human CYP11B2 expressed in V79MZh hamster fibroblasts incubated with 100 nM of substrate deoxy-corticosterone in presence of the compound	B	9	pIC50	1	nM	IC50	J Med Chem (2005) 48: 1563-1575 [PMID:15743198]
ChEMBL	Inhibitory concentration against human cytochrome P450 11B2 expressed in fission yeast, incubated with [14C]deoxycorticosterone	B	9	pIC50	1	nM	IC50	J Med Chem (2005) 48: 6632-6642 [PMID:16220979]
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	9	pIC50	1	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	9	pIC50	1	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	9	pIC50	1	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition Assay: V79 MZh11B1 and V79 MZh 11B2 cells (8˙10^5 cells per well) were grown to confluency on 24-well cell culture plates with 1.9 cm^2 culture area per well (Nunc, Roskilde, Denmark). Before the test, the DMEM culture medium present was removed, and 450 μl of fresh DMEM with inhibitor was added for at least three concentrations to each well to determine the IC50. After preincubation (60 min, 37 °C.), the reaction was started by adding 50 μl of DMEM with 2.5 μl of solution of the substrate 11-deoxycorticosterone (20 μM, containing 1.25 nCi of [4-14C]11-deoxycorticosterone in ethanol). Thereafter, the plate was stored at 37 °C. and 5% CO2 in a CO2 incubator. The V79 MZh 11B1 cells were incubated for 120 min, and the V79 MZh 11B2 cells were incubated for 40 min. Controls without inhibitor were treated in the same way. The enzyme reactions were quenched by extracting the supernatant with 500 μl of EtOAc. The samples were centrifuged (10,000 g, 2 min), the solvent was removed and evaporated. The residue was taken up in 10 μl of chloroform and analyzed by HPTLC (see below).	B	9	pIC50	1	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition of human CYP11B2 expressed in V79 MZh cells using [14C]-deoxycorticosterone substrate incubated for 6 hrs by HPTLC method	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2015) 58: 2530-2537 [PMID:25711516]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2013) 56: 1723-1729 [PMID:23363058]
ChEMBL	Inhibition of human CYP11B2 expressed in V79MZh cells using [14C]-11-deoxycorticosterone as substrate by HPTLC/phosphoimaging method	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2012) 55: 7080-7089 [PMID:22861193]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxy-corticosterone as substrate	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2013) 56: 6101-6107 [PMID:23859149]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using deoxycorticosterone as substrate	B	9.1	pIC50	0.8	nM	IC50	J Med Chem (2014) 57: 5179-5189 [PMID:24899257]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZ cells using [3H]-11-deoxycorticosterone as substrate after 1 hr by HPLC analysis	B	9.1	pIC50	0.8	nM	IC50	Eur J Med Chem (2015) 89: 597-605 [PMID:25462268]
ChEMBL	Inhibition of CYP11B2 in human V79MZ cells using [3H]-11-deoxycorticosterone as substrate incubated for 1 hr prior to substrate addition measured after 45 mins by HPLC analysis	B	9.1	pIC50	0.8	nM	IC50	Eur J Med Chem (2015) 90: 788-796 [PMID:25528333]
CYP11B2/Cytochrome P450 11B2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3237] [GtoPdb: 1360] [UniProtKB: P30099]
ChEMBL	In vitro inhibition of ACTH-stimulated aldosterone biosynthesis in rat adrenal slices	F	6.22	pIC50	600	nM	IC50	J Med Chem (1995) 38: 2103-2111 [PMID:7783141]
CYP11B2 in Human [GtoPdb: 1360] [UniProtKB: P19099]
GtoPdb	-	-	9.1	pKi	0.8	nM	Ki	Anal Biochem (2009) 394: 56-61 [PMID:19622340]
CYP11B2/Cytochrome P450 11B2, mitochondrial in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3621020] [GtoPdb: 1360] [UniProtKB: P15539]
ChEMBL	Inhibition of mouse CYP11B2 expressed in renal leiomyoblastoma cells	B	6.72	pIC50	190	nM	IC50	J Med Chem (2015) 58: 8054-8065 [PMID:26403853]
CYP17A1/Cytochrome P450 17A1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3522] [GtoPdb: 1361] [UniProtKB: P05093]
ChEMBL	Inhibition of human CYP17 expressed in Escherichia coli co-expressing rat NADPH-P450-reductase using progesterone as substrate	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2013) 56: 1723-1729 [PMID:23363058]
CYP19A1/Cytochrome P450 19A1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1978] [GtoPdb: 1362] [UniProtKB: P11511]
ChEMBL	Inhibition of aromatase in human placental microsomes	B	8.8	pKi	1.6	nM	Ki	Eur J Med Chem (2022) 241: 114658-114658 [PMID:35964426]
ChEMBL	Binding affinity was measured on Cytochrome P450 19A1	B	8.82	pKi	1.5	nM	Ki	J Med Chem (1990) 33: 2933-2942 [PMID:2231592]
ChEMBL	Inhibition constant for human aromatase cytochrome P450 19A1 activity	B	9.22	pKi	0.6	nM	Ki	Bioorg Med Chem Lett (1998) 8: 1041-1044 [PMID:9871704]
ChEMBL	Competitive inhibition of human aromatase using dibenzylfluorescein substrate after 10 mins preincubation measured every 10 sec for 5 mins by Michaelis-Menten and Dixon plot analysis	B	10.3	pKi	0.05	nM	Ki	Bioorg Med Chem (2012) 20: 2427-2434 [PMID:22386564]
ChEMBL	Inhibition of human aromatase cytochrome P450 19A1 activity	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem Lett (1998) 8: 1041-1044 [PMID:9871704]
ChEMBL	Inhibition of human placental microsome cytochrome P450 19A1 aromatase	B	7.28	pIC50	52	nM	IC50	J Med Chem (2001) 44: 672-680 [PMID:11262078]
ChEMBL	Inhibition of CYP19 in human placental microsomes using [1beta-3H]-androstendione as a substrate	B	7.28	pIC50	52	nM	IC50	J Med Chem (2012) 55: 7080-7089 [PMID:22861193]
ChEMBL	Inhibition of human placental microsome CYP19 using [1beta-3H] androstenedione as a substrate	B	7.28	pIC50	52	nM	IC50	J Med Chem (2011) 54: 1613-1625 [PMID:21341743]
ChEMBL	Inhibition of aromatase from human placental microsomes	B	7.28	pIC50	52	nM	IC50	J Med Chem (2010) 53: 5347-5351 [PMID:20568782]
ChEMBL	Inhibition of CYP19 in human placental microsomes	B	7.28	pIC50	52	nM	IC50	J Med Chem (2007) 50: 3420-3422 [PMID:17585752]
ChEMBL	Inhibition of CYP19	B	7.28	pIC50	52	nM	IC50	J Med Chem (2006) 49: 4777-4780 [PMID:16854084]
ChEMBL	Inhibition of human aromatase	B	7.3	pIC50	50	nM	IC50	Bioorg Med Chem (2008) 16: 8349-8358 [PMID:18782670]
ChEMBL	Inhibition of human placental CYP19 using [1beta-3H]androstenedione as substrate by 3H2O-method	B	7.39	pIC50	41	nM	IC50	J Med Chem (2013) 56: 1723-1729 [PMID:23363058]
ChEMBL	Inhibition of human placental CYP19 using androstenedione substrate	B	7.39	pIC50	41	nM	IC50	J Med Chem (2012) 55: 6629-6633 [PMID:22788843]
ChEMBL	Inhibition of human CYP19 using [1beta-3H]androstenedione as substrate by 3H2O method	B	7.39	pIC50	41	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
ChEMBL	In vitro inhibition of cytochrome P450 19A1	B	7.41	pIC50	39	nM	IC50	J Med Chem (1991) 34: 725-736 [PMID:1825337]
ChEMBL	Inhibitory concentration against human placental cytochrome P450 19A1 using [1-beta-3H]-androstenedione as substrate	B	7.52	pIC50	30	nM	IC50	J Med Chem (2005) 48: 6632-6642 [PMID:16220979]
ChEMBL	Inhibition of human placental CYP19	F	7.52	pIC50	30	nM	IC50	J Med Chem (2008) 51: 8077-8087 [PMID:19049427]
ChEMBL	CYP19 Inhibition Assay: The enzyme was obtained from the microsome fraction of fresh human placenta (St. Josephs Krankenhaus, Saarbrucken-Dudweiler, Germany) according to the method of Thompson and Siiteri (Thompson, E. A. & Siiteri, P. K., J. Biol. Chem. 249: 5364-5372 (1974)). The isolated microsomes were suspended in a mini mum volume of phosphate buffer (0.05 M; pH 7.4; 20% glycerol). In addition, DTT (10 mM) and EDTA (1 mM) were added to protect the enzyme from degradation reactions. The protein concentration was determined according to Lowry et al. (Lowry, O. H. et al., J. Biol. Chem. 193: 265-275 (1951)) and should be about 35 mg/ml after the processing.	B	7.52	pIC50	30	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	In vitro inhibitory concentration against human placental CYP19 incubated with 500 nM of substrate androstenedione in presence of the compound	B	7.52	pIC50	30	nM	IC50	J Med Chem (2005) 48: 1563-1575 [PMID:15743198]
ChEMBL	Inhibition of human placental CYP19 using androstenedione as substrate	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem Lett (2011) 21: 186-190 [PMID:21129965]
ChEMBL	CYP19 Inhibition Assay: The enzyme was obtained from the microsome fraction of fresh human placenta (St. Josephs Krankenhaus, Saarbrucken-Dudweiler, Germany) according to the method of Thompson and Siiteri (Thompson, E. A. & Siiteri, P. K., J. Biol. Chem. 249: 5364-5372 (1974)). The isolated microsomes were suspended in a mini mum volume of phosphate buffer (0.05 M; pH 7.4; 20% glycerol). In addition, DTT (10 mM) and EDTA (1 mM) were added to protect the enzyme from degradation reactions. The protein concentration was determined according to Lowry et al. (Lowry, O. H. et al., J. Biol. Chem. 193: 265-275 (1951)) and should be about 35 mg/ml after the processing.	B	7.52	pIC50	30	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition of human placental CYP19 using [1beta-3H]androstenedione substrate	B	7.52	pIC50	30	nM	IC50	J Med Chem (2011) 54: 2307-2319 [PMID:21384875]
ChEMBL	Inhibition of human recombinant CYP19 using [1beta-3H]androstenedione as substrate	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2015) 90: 788-796 [PMID:25528333]
ChEMBL	Inhibition of human placental CYP19	B	7.52	pIC50	30	nM	IC50	J Med Chem (2006) 49: 2222-2231 [PMID:16570918]
ChEMBL	Inhibition of human placental microsome CYP19	B	7.52	pIC50	30	nM	IC50	J Med Chem (2008) 51: 6138-6149 [PMID:18763754]
ChEMBL	Inhibition of human placental CYP19	B	7.52	pIC50	30	nM	IC50	J Med Chem (2008) 51: 5064-5074 [PMID:18672861]
ChEMBL	CYP19 Inhibition Assay: The enzyme was obtained from the microsome fraction of fresh human placenta (St. Josephs Krankenhaus, Saarbrucken-Dudweiler, Germany) according to the method of Thompson and Siiteri (Thompson, E. A. & Siiteri, P. K., J. Biol. Chem. 249: 5364-5372 (1974)). The isolated microsomes were suspended in a mini mum volume of phosphate buffer (0.05 M; pH 7.4; 20% glycerol). In addition, DTT (10 mM) and EDTA (1 mM) were added to protect the enzyme from degradation reactions. The protein concentration was determined according to Lowry et al. (Lowry, O. H. et al., J. Biol. Chem. 193: 265-275 (1951)) and should be about 35 mg/ml after the processing.	B	7.53	pIC50	29.5	nM	IC50	US-9271963-B2. Selective inhibitors of human corticosteroid synthases (2016)
ChEMBL	Inhibition of human placental microsome CYP19	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3050-3064 [PMID:20413308]
ChEMBL	In vitro inhibitory concentration against human placental cytochrome P450 19 with 2.5 uM testosterone	B	8.3	pIC50	5	nM	IC50	J Med Chem (2005) 48: 1796-1805 [PMID:15771425]
ChEMBL	In vitro inhibition of cytochrome P450 19A1	B	8.34	pIC50	4.6	nM	IC50	J Med Chem (1991) 34: 725-736 [PMID:1825337]
ChEMBL	Inhibition of human placental microsome CYP19	B	8.35	pIC50	4.5	nM	IC50	J Med Chem (2014) 57: 5011-5022 [PMID:24422519]
GtoPdb	-	-	8.35	pIC50	4.5	nM	IC50	J Med Chem (1991) 34: 725-36 [PMID:1825337]
ChEMBL	Inhibition of human aromatase using dibenzylfluorescein substrate preincubated for 30 mins measured after 30 mins by fluorescence assay	B	8.52	pIC50	3	nM	IC50	Bioorg Med Chem (2012) 20: 2427-2434 [PMID:22386564]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]