(R)-CR8   Click here for help

GtoPdb Ligand ID: 11065

PDB Ligand
Compound class: Synthetic organic
Comment: CR8 is a small molecule inhibitor of cyclin-dependent kinases [1]. It is structurally related to seliciclib (roscovitine). This entry is for the R isomer, which is more inhibitory at glycogen synthase kinase (GSK-3α/β) than the S isomer. Both isomers are equipotent across CDK1, 2, 5, 7, and 9.

(R)-CR8 has been shown to act as a molecular glue degrader that depletes cyclin K [2], thus behaving as a functional CDK inhibitor. (R)-CR8 binding to CDK12 induces formation of a complex between CDK12-cyclin K and the CUL4 adaptor protein DDB1 which presents cyclin K for ubiquitination and degradation. Of the >8,000 proteins assessed by quantitative proteome-wide mass spectrometry, cyclin K was the only one that was consistently decreased in abundance in response to (R)-CR8 exposure.
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2D Structure
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Physico-chemical Properties
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Hydrogen bond acceptors 7
Hydrogen bond donors 3
Rotatable bonds 9
Topological polar surface area 100.78
Molecular weight 431.24
XLogP 4.28
No. Lipinski's rules broken 0
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Canonical SMILES CC[C@@H](Nc1nc(NCc2ccc(cc2)c2ccccn2)c2c(n1)n(cn2)C(C)C)CO
Isomeric SMILES CC[C@@H](Nc1nc(NCc2ccc(cc2)c2ccccn2)c2c(n1)n(cn2)C(C)C)CO
InChI InChI=1S/C24H29N7O/c1-4-19(14-32)28-24-29-22(21-23(30-24)31(15-27-21)16(2)3)26-13-17-8-10-18(11-9-17)20-7-5-6-12-25-20/h5-12,15-16,19,32H,4,13-14H2,1-3H3,(H2,26,28,29,30)/t19-/m1/s1
1. Bettayeb K, Oumata N, Echalier A, Ferandin Y, Endicott JA, Galons H, Meijer L. (2008)
CR8, a potent and selective, roscovitine-derived inhibitor of cyclin-dependent kinases.
Oncogene, 27 (44): 5797-807. [PMID:18574471]
2. Słabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, Donovan KA, Sievers QL, Koeppel J, Suchyta D et al.. (2020)
The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K.
Nature, 585 (7824): 293-297. [PMID:32494016]