Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Plasmodium falciparum 3D7	-	734		IspD	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, putative

Previous and Unofficial Names

IspD | Pf3D7_01_v3:289,849..292,802(+) | PFA0340w

Database Links
Alphafold	Q8I273 (Pf3D7)
PlasmoDB	PF3D7_0106900 (Pf3D7)
UniProtKB	Q8I273 (Pf3D7)

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Inhibitors
Key to terms and symbols	View all chemical structures	Click column headers to sort
Ligand Sp. Action Value Parameter Reference MMV008138 Pf Plasmodium falciparum P. falciparum (Pf) is one of five protozoan parasite species of the genus Plasmodium that cause malaria in humans. Pf is responsible for the majority of malaria related deaths and is the most prevalent species in sub-Saharan Africa. ✖ Inhibition 8.1 pIC50 2 ⤷ pIC50 8.1 (IC50 7x10-9 M) [2] Description: Enzymatic activity measured using a pyrophosphate release assay

Whole Organism Assay Data Linked to This Target
Key to terms and symbols		Click column headers to sort
Ligand Sp. Assay description Type Action Value Parameter Reference MMV008138 PfW2 Plasmodium falciparum W2 P. falciparum strain W2 (PfW2) was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy. PfW2 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and susceptible to mefloquine. ✖ Parasite growth inhibition assay - - 7.0 pEC50 2 ⤷ pEC50 7.0 (EC50 1.1x10-7 M) [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) MMV008138 PfDd2 Plasmodium falciparum Dd2 P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy. PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine. ✖ Parasite growth inhibition assay - - 6.6 pIC50 3 ⤷ pIC50 6.6 (IC50 2.5x10-7 M) SYBR green assay [3] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)

Malaria Pharmacology Comments

The non-mevalonate (MEP) pathway of isoprenoid precursor biosynthesis is utilized by bacteria, plants and apicomplexan protozoa but is not present in mammals (complete pathway for P. falciparum is available at www.wikipathways.org). Enzymes of this pathway represent promising therapeutic targets for antimalarial drug development because the pathway is present in the Plasmodium parasite but not in the human host [1]. 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (PfIspD) is the third enzyme in the pathway, following PfDXR.