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Kir2.4

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Target not currently curated in GtoImmuPdb

Target id: 433

Nomenclature: Kir2.4

Family: Inwardly rectifying potassium channels (KIR)

Contents:

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 2 1 436 19q13.33 KCNJ14 potassium inwardly rectifying channel subfamily J member 14 2,8
Mouse 2 1 434 7 B3 Kcnj14 potassium inwardly-rectifying channel, subfamily J, member 14 6
Rat 2 1 434 1q22 Kcnj14 potassium inwardly-rectifying channel, subfamily J, member 14 9
Previous and Unofficial Names Click here for help
potassium inwardly rectifying channel subfamily J member 14 | ATP-sensitive inward rectifier potassium channel 14 | IRK4 | potassium channel, inwardly rectifying subfamily J, member 14 | potassium inwardly-rectifying channel | potassium voltage-gated channel subfamily J member 14
Database Links Click here for help
Alphafold Q9UNX9 (Hs), Q8JZN3 (Mm), O70596 (Rn)
CATH/Gene3D 2.60.40.1400
Ensembl Gene ENSG00000182324 (Hs), ENSMUSG00000058743 (Mm), ENSRNOG00000021056 (Rn)
Entrez Gene 3770 (Hs), 211480 (Mm), 276720 (Rn)
Human Protein Atlas ENSG00000182324 (Hs)
KEGG Gene hsa:3770 (Hs), mmu:211480 (Mm), rno:276720 (Rn)
OMIM 603953 (Hs)
Pharos Q9UNX9 (Hs)
RefSeq Nucleotide NM_013348 (Hs), NM_145963 (Mm), NM_170718 (Rn)
RefSeq Protein NP_037480 (Hs), NP_666075 (Mm), NP_733836 (Rn)
UniProtKB Q9UNX9 (Hs), Q8JZN3 (Mm), O70596 (Rn)
Wikipedia KCNJ14 (Hs)
Associated Proteins Click here for help
Heteromeric Pore-forming Subunits
Name References
Kir2.1 5
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
Not determined
Functional Characteristics Click here for help
IK1 in heart, ‘strong’ inward–rectifier current
Ion Selectivity and Conductance Click here for help
Species:  Rat
Rank order:  K+ [15.0 - 19.4 pS]
References:  7,9

Download all structure-activity data for this target as a CSV file go icon to follow link

Inhibitors
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
Intracellular Mg2+ Click here for species-specific activity table Ligand is endogenous in the given species Hs - - - - -
⤷
Gating inhibitors Click here for help
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
Extracellular H+ Click here for species-specific activity table Ligand is endogenous in the given species Hs Antagonist 7.1 pKi - -120.0 2
⤷ pKi 7.1 [2]
Holding voltage: -120.0 mV
Gating Inhibitor Comments
Endogenous inhibitors are intracellular Mg2+ and polyamines (spermine4+, spermidine3+, putrescine2+).
Channel Blockers
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
Cs+ Click here for species-specific activity table Hs Antagonist 3.0 – 4.1 pKd - -60.0 – -100.0 2
⤷ pKd 3.0 – 4.1 [2]
Holding voltage: -60.0 – -100.0 mV
Ba2+ Click here for species-specific activity table Hs Antagonist 3.3 pKd - 0.0 2
⤷ pKd 3.3 [2]
Holding voltage: 0.0 mV
Ba2+ Click here for species-specific activity table Rn Antagonist 3.4 – 4.2 pKi 1x10-6 - 1x10-3 -80.0 – -100.0 7,9
⤷ pKi 3.4 – 4.2 Conc range: 1x10-6 - 1x10-3 M [7,9]
Holding voltage: -80.0 – -100.0 mV
Cs+ Click here for species-specific activity table Rn Antagonist 2.1 pKi - -80.0 9
⤷ pKi 2.1 [9]
Holding voltage: -80.0 mV
View species-specific channel blocker tables
Tissue Distribution Click here for help
Retina.
Species:  Human
Technique:  Northern Blot
References:  2
Cultured pulmonary smooth muscle cells.
Species:  Human
Technique:  RT-PCR
References:  7
Aortic endothelial cells (mRNA, but not protein, can be detected in this tissue).
Species:  Human
Technique:  RT-PCR
References:  1
Cranial nerve motor nuclei in midbrain, pons and medulla (cholinergic neurons).
Species:  Rat
Technique:  In situ hybridisation, immunohistochemistry.
References:  3,9
Striatum (cholinergic interneurons).
Species:  Rat
Technique:  Immunohistochemistry
References:  4
Functional Assays Click here for help
Kir2.1 and Kir2.4 contribute to inward rectifier potassium current in cultured human pulmonary smooth muscle cells.
Species:  None
Tissue:  Cultured pulmonary smooth muscle cells.
Response measured:  Measurement of K+ current.
References:  7
Control of the excitability of brainstem motorneurons by setting the membrane potential near EK.
Species:  Rat
Tissue:  Hypoglossal motorneurons.
Response measured:  Spike discharge.
References:  9
Physiological Functions Click here for help
Controls the excitability of brainstem motorneurons by setting the membrane potential near EK.
Species:  Rat
Tissue:  Hypoglossal motorneurons.
References:  9

References

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1. Fang Y, Schram G, Romanenko VG, Shi C, Conti L, Vandenberg CA, Davies PF, Nattel S, Levitan I. (2005) Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2. Am J Physiol, Cell Physiol, 289 (5): C1134-44. [PMID:15958527]

2. Hughes BA, Kumar G, Yuan Y, Swaminathan A, Yan D, Sharma A, Plumley L, Yang-Feng TL, Swaroop A. (2000) Cloning and functional expression of human retinal kir2.4, a pH-sensitive inwardly rectifying K(+) channel. Am J Physiol, Cell Physiol, 279 (3): C771-84. [PMID:10942728]

3. Prüss H, Derst C, Lommel R, Veh RW. (2005) Differential distribution of individual subunits of strongly inwardly rectifying potassium channels (Kir2 family) in rat brain. Brain Res Mol Brain Res, 139 (1): 63-79. [PMID:15936845]

4. Prüss H, Wenzel M, Eulitz D, Thomzig A, Karschin A, Veh RW. (2003) Kir2 potassium channels in rat striatum are strategically localized to control basal ganglia function. Brain Res Mol Brain Res, 110 (2): 203-19. [PMID:12591157]

5. Schram G, Melnyk P, Pourrier M, Wang Z, Nattel S. (2002) Kir2.4 and Kir2.1 K(+) channel subunits co-assemble: a potential new contributor to inward rectifier current heterogeneity. J Physiol (Lond.), 544 (2): 337-49. [PMID:12381809]

6. Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF et al.. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci USA, 99 (26): 16899-903. [PMID:12477932]

7. Tennant BP, Cui Y, Tinker A, Clapp LH. (2006) Functional expression of inward rectifier potassium channels in cultured human pulmonary smooth muscle cells: evidence for a major role of Kir2.4 subunits. J Membr Biol, 213 (1): 19-29. [PMID:17347781]

8. Töpert C, Döring F, Derst C, Daut J, Grzeschik KH, Karschin A. (2000) Cloning, structure and assignment to chromosome 19q13 of the human Kir2.4 inwardly rectifying potassium channel gene (KCNJ14). Mamm Genome, 11 (3): 247-9. [PMID:10723734]

9. Töpert C, Döring F, Wischmeyer E, Karschin C, Brockhaus J, Ballanyi K, Derst C, Karschin A. (1998) Kir2.4: a novel K+ inward rectifier channel associated with motoneurons of cranial nerve nuclei. J Neurosci, 18 (11): 4096-105. [PMID:9592090]

Contributors

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