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Kv12.3

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Target not currently curated in GtoImmuPdb

Target id: 577

Nomenclature: Kv12.3

Family: Voltage-gated potassium channels

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 1017 17q21.2 KCNH4 potassium voltage-gated channel subfamily H member 4
Mouse 6 1 1018 11 D Kcnh4 potassium voltage-gated channel, subfamily H (eag-related), member 4
Rat 6 1 1017 10q31 Kcnh4 potassium voltage-gated channel subfamily H member 4
Previous and Unofficial Names Click here for help
BEC2 | Elk1 | brain-specific eag-like channel 2 | ELK channel 1 | ether-a-go-go-like potassium channel 1 | potassium voltage-gated channel, subfamily H (eag-related), member 4 | potassium channel, voltage gated eag related subfamily H, member 4 | potassium voltage-gated channel
Database Links Click here for help
CATH/Gene3D
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
Pharos
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Ion Selectivity and Conductance Click here for help
Species:  Rat
Macroscopic current rectification:  Ik
References:  1
Voltage Dependence Click here for help
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  -59.0 38.0 1 CHO cells. Rat
Inactivation  - -
Deactivation  - 200.0 1
Comments  τ deactivation at -80mV [1]
Gating Inhibitor Comments
Voltage clamp of Kv12.3 heterologously expressed in HEK293 cells demonstrated that external H+ is a gating inhibitor with a pKa of 7.1 and holding voltage -60mV [2].
Channel Blocker Comments
At millimolar concentrations, Ba2+ acts as a pore blocker for Kv12.3 [1].
Kv12.3 is resistant to block by external TEA, 4-AP, E-4031 [1].
Tissue Distribution Click here for help
Cerebral cortex, occipital pole, fromtal lobe, tenporal lobe, putamen, caudate nucleus, hippocampus.
Species:  Human
Technique:  Northern blot
References:  3
Piriform cortex, olfactory tubercle, cerebral cortex layers II-VI, hippocampus, basal ganglia
Species:  Rat
Technique:  In situ hybridisation
References:  4
Pituitary and clonal somato-mammotroph cells (GH3 / B6 cells)
Species:  Rat
Technique:  RT-PCR
References:  5
Lung, spinal cord, hippocampus, cortex, striatum, pituitary, medulla
Species:  Rat
Technique:  RT-PCR
References:  1
Brain, testes
Species:  Rat
Technique:  Northern blot
References:  1
Functional Assays Click here for help
Membrane currents recorded from heterologously expressed channels using whole-cell patch clamp
Species:  Rat
Tissue:  CHO (Chinese Hamster Ovary) cells
Response measured:  Remarkably low threshold of activation, may contribute to the maintenance of the resting potential. Activation kinetics not dependent on prepulse potential.
References:  1
General Comments
This channel has:
  • GFG (rather than the common GYG) potassium channel signature sequence
  • a PAS domain in the distal part of the cytosolic N-terminus
  • a cNBD domain in the proximal portion of the C-terminus

References

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1. Engeland B, Neu A, Ludwig J, Roeper J, Pongs O. (1998) Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. J Physiol (Lond.), 513 ( Pt 3): 647-54. [PMID:9824707]

2. Kazmierczak M, Zhang X, Chen B, Mulkey DK, Shi Y, Wagner PG, Pivaroff-Ward K, Sassic JK, Bayliss DA, Jegla T. (2013) External pH modulates EAG superfamily K+ channels through EAG-specific acidic residues in the voltage sensor. J Gen Physiol, 141 (6): 721-35. [PMID:23712551]

3. Miyake A, Mochizuki S, Yokoi H, Kohda M, Furuichi K. (1999) New ether-à-go-go K(+) channel family members localized in human telencephalon. J Biol Chem, 274 (35): 25018-25. [PMID:10455180]

4. Saganich MJ, Machado E, Rudy B. (2001) Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain. J Neurosci, 21 (13): 4609-24. [PMID:11425889]

5. Wulfsen I, Hauber HP, Schiemann D, Bauer CK, Schwarz JR. (2000) Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary. J Neuroendocrinol, 12 (3): 263-72. [PMID:10718922]

Contributors

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