ChEMBL ligand: CHEMBL279453 (AF-DX-384)
Muscarinic acetylcholine receptor DM1 in Drosophila melanogaster [ChEMBL: CHEMBL2366467] [UniProtKB: P16395] There should be some charts here, you may need to enable JavaScript!
M1 receptor/Muscarinic acetylcholine receptor M1 in Human [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229] There should be some charts here, you may need to enable JavaScript!
M2 receptor/Muscarinic acetylcholine receptor M2 in Human [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172] There should be some charts here, you may need to enable JavaScript!
M3 receptor/Muscarinic acetylcholine receptor M3 in Human [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309] There should be some charts here, you may need to enable JavaScript!
M4 receptor/Muscarinic acetylcholine receptor M4 in Human [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173] There should be some charts here, you may need to enable JavaScript!
M5 receptor/Muscarinic acetylcholine receptor M5 in Human [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Muscarinic acetylcholine receptor DM1 in Drosophila melanogaster (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366467] [UniProtKB: P16395]
ChEMBL	Displacement of [3H]AF-DX 384 from Drosophila melanogaster mAChR by scintillation counting	B	8.21	pKi	6.1	nM	Ki	J Agric Food Chem (2007) 55: 2276-2281 [PMID:17319687]
ChEMBL	Displacement of [3H]QNB from Drosophila melanogaster mAChR by scintillation counting	B	8.32	pKi	4.8	nM	Ki	J Agric Food Chem (2007) 55: 2276-2281 [PMID:17319687]
M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
ChEMBL	Affinity for Muscarinic acetylcholine receptor M1 expressed in CHO cells by [3H]NMS displacement.	B	7.51	pKi	30.9	nM	Ki	Bioorg. Med. Chem. Lett. (2001) 11: 1241-1243 [PMID:11354386]
ChEMBL	Displacement of [3H]-NMS from human muscarinic M1 receptor expressed in stable CHO-K1 cells by radioligand binding assay	B	7.51	pKi	30.9	nM	Ki	J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL	Compound was evaluated for displacement of [3H]QNB from human Muscarinic m1 receptor in CHO cells.	B	7.91	pKi	12.4	nM	Ki	Bioorg. Med. Chem. Lett. (1996) 6: 785-788
M2 receptor/Muscarinic acetylcholine receptor M2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172]
GtoPdb	-	-	9	pKd	-	-	-	J Pharmacol Exp Ther (1991) 259: 601-7 [PMID:1941609]; Front Pharmacol (2018) 9: 124 [PMID:29515448]; Brain Sci (2020) 10: [PMID:32079174]
ChEMBL	Affinity for Muscarinic acetylcholine receptor M2 expressed in CHO cells by [3H]NMS displacement.	B	8.22	pKi	6.03	nM	Ki	Bioorg. Med. Chem. Lett. (2001) 11: 1241-1243 [PMID:11354386]
ChEMBL	Displacement of [3H]-NMS from human muscarinic M2 receptor expressed in stable CHO-K1 cells by radioligand binding assay	B	8.22	pKi	6.03	nM	Ki	J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL	Compound was evaluated for displacement of [3H]-QNB from human Muscarinic m2 receptor in CHO cells.	B	8.28	pKi	5.28	nM	Ki	Bioorg. Med. Chem. Lett. (1996) 6: 785-788
M3 receptor/Muscarinic acetylcholine receptor M3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309]
ChEMBL	Affinity for Muscarinic acetylcholine receptor M3 expressed in CHO cells by [3H]-NMS displacement.	B	7.18	pKi	66.07	nM	Ki	Bioorg. Med. Chem. Lett. (2001) 11: 1241-1243 [PMID:11354386]
ChEMBL	Displacement of [3H]-NMS from human muscarinic M3 receptor expressed in stable CHO-K1 cells by radioligand binding assay	B	7.18	pKi	66.07	nM	Ki	J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
M4 receptor/Muscarinic acetylcholine receptor M4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173]
GtoPdb	-	-	8.7	pKd	-	-	-	J Pharmacol Exp Ther (1991) 259: 601-7 [PMID:1941609]; Front Pharmacol (2018) 9: 124 [PMID:29515448]; Brain Sci (2020) 10: [PMID:32079174]
ChEMBL	Affinity for Muscarinic acetylcholine receptor M4 expressed in CHO cells by [3H]NMS displacement.	B	8	pKi	10	nM	Ki	Bioorg. Med. Chem. Lett. (2001) 11: 1241-1243 [PMID:11354386]
ChEMBL	Displacement of [3H]-NMS from human muscarinic M4 receptor expressed in stable CHO-K1 cells by radioligand binding assay	B	8	pKi	10	nM	Ki	J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
M5 receptor/Muscarinic acetylcholine receptor M5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912]
ChEMBL	Affinity for Muscarinic acetylcholine receptor M5 expressed in CHO cells by [3H]NMS displacement.	B	6.27	pKi	537.03	nM	Ki	Bioorg. Med. Chem. Lett. (2001) 11: 1241-1243 [PMID:11354386]
ChEMBL	Displacement of [3H]-NMS from human muscarinic M5 receptor expressed in stable CHO-K1 cells by radioligand binding assay	B	6.27	pKi	537.03	nM	Ki	J Med Chem (2019) 62: 5358-5369 [PMID:31074983]

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]