olaparib [Ligand Id: 7519] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL521686 (OLAPARIB COMPONENT OF KEYLYNK-010, AZ2281, Olaparib, AZ-2281, AZD2281, AZD-2281, KEYLYNK-010 COMPONENT OLAPARIB, KU-59436, KU-0059436, Lynparza)
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  • poly(ADP-ribose) polymerase 1/Poly [ADP-ribose] polymerase-1 in Human [ChEMBL: CHEMBL3105] [GtoPdb: 2771] [UniProtKB: P09874]
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  • poly(ADP-ribose) polymerase 2/Poly [ADP-ribose] polymerase 2 in Human [ChEMBL: CHEMBL5366] [GtoPdb: 2772] [UniProtKB: Q9UGN5]
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  • poly (ADP-ribose) polymerase 3/Poly [ADP-ribose] polymerase 3 in Human [ChEMBL: CHEMBL5083] [GtoPdb: 2864] [UniProtKB: Q9Y6F1]
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  • Poly [ADP-ribose] polymerase 4 in Human [ChEMBL: CHEMBL6142] [UniProtKB: Q9UKK3]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Mono [ADP-ribose] polymerase PARP16 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105981] [UniProtKB: Q8N5Y8]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP16 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.29 pIC50 5128.61 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP16 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.29 pIC50 5100 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
poly(ADP-ribose) polymerase 1/Poly [ADP-ribose] polymerase-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3105] [GtoPdb: 2771] [UniProtKB: P09874]
ChEMBL Binding affinity to recombinant human HisGST-tagged PARP-1 catalytic domain by surface plasmon resonance analysis B 9.62 pKd 0.24 nM Kd J. Med. Chem. (2015) 58: 6875-6898 [PMID:26222319]
ChEMBL Binding affinity to full length His-tagged PARP1 (unknown origin) after 11 mins in presence of NAD+ by TR-FRET assay B 9.1 pKi 0.79 nM Ki MedChemComm (2019) 10: 974-984
ChEMBL Inhibition of N-terminal GST-tagged human PARP1 expressed in a Baculovirus infected Sf9 insect cells using biotinylated substrate incubated for 1 hr by colorimetric method B 6.86 pIC50 139 nM IC50 Bioorg Med Chem (2019) 27: 3979-3997 [PMID:31401008]
ChEMBL Cellular Assay: Based on the preliminary PARP1 inhibition evaluation of compounds at molecular level by ELISA, compounds were further evaluated for their cellular inhibition against PARP1 using a proliferation inhibition model. B 7.06 pIC50 86.32 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of human PARP1 B 7.07 pIC50 84.96 nM IC50 Bioorg. Med. Chem. Lett. (2014) 24: 3739-3743 [PMID:25086680]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 C-3-zinc finger domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.16 pIC50 69.18 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL ELISA Assay: The HTb-PARP1 positive clones were obtained using the full-length PARP1 plasmid, through PCR amplification, enzyme digestion, ligation, and transformation into DH5a. The plasmids were extracted and determined by enzyme cleavage, and then transformed into DH10Bac. Bacmid/PARP is determined by PCR and sequencing. TNI was transfected, the viruses were collected, and cells were lysed. PARP1 protein was purified by affinity chromatography and determined by Western blotting. A plate was coated by substrate histone, NAD+ and DNA, as well as expressed PARP1 enzyme, was placed into 96-well plate reaction system. Various reaction conditions were optimized and ultimately determined. B 7.3 pIC50 <50 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL ELISA Assay: The HTb-PARP1 positive clones were obtained using the full-length PARP1 plasmid, through PCR amplification, enzyme digestion, ligation, and transformation into DH5a. The plasmids were extracted and determined by enzyme cleavage, and then transformed into DH10Bac. Bacmid/PARP is determined by PCR and sequencing. TNI was transfected, the viruses were collected, and cells were lysed. PARP1 protein was purified by affinity chromatography and determined by Western blotting. A plate was coated by substrate histone, NAD+ and DNA, as well as expressed PARP1 enzyme, was placed into 96-well plate reaction system. Various reaction conditions were optimized and ultimately determined. B 7.37 pIC50 43 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of recombinant human full length C-terminal His-tagged PARP1 expressed in Sf9 insect cells preincubated for 15 mins followed by NAD+ addition and measured after 40 mins by fluorescence based assay B 7.44 pIC50 36 nM IC50 Bioorg Med Chem Lett (2019) 29: 1904-1908 [PMID:31153806]
ChEMBL Inhibition of PARP1 (unknown origin) assessed as reduction in biotinylated poly(ADP-ribose) incorporation on to histone protein by ELISA based colorimetric assay B 7.7 pIC50 20 nM IC50 Eur J Med Chem (2017) 138: 1135-1146 [PMID:28763648]
ChEMBL Inhibition of PARP1 (unknown origin) by ELISA B 7.72 pIC50 19.1 nM IC50 Eur J Med Chem (2017) 138: 514-531 [PMID:28692916]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 C-3-zinc finger domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.72 pIC50 18.9 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of PARP-1 (unknown origin) assessed as incorporation of biotinylated poly ADP-ribose onto histone proteins by colorimetric analysis in presence of GSH and GSTP1 B 7.81 pIC50 15.5 nM IC50 J. Med. Chem. (2014) 57: 2292-2302 [PMID:24521039]
ChEMBL In Vitro Screening Assay: PARP-1 can catalyze the NAD-dependent addition of poly (ADP-ribose) to adjacent nuclear proteins such as histone. The activity of PARP was determined by detecting biotin coupled ADP-ribose that attaches to histone using chemiluminescence method with HT Universal Chemiluminescent PARP Assay Kit With Histone-coated Strip Wells purchased from TREVIGEN. The positive control compound was AZD-2281 purchased from SelleckChem.Method: The kit is based on ELISA. X ul sample/well, Y ul PARP enzyme/well and PARP cocktail 25 ul/well with a total volume of 50 ul/well were added into a 96-well plate coated with histone. Meanwhile, blank control (without enzyme and sample) and negative control (without sample) were set. The plate was incubated at room temperature for 60 min; washed with 1xPBS (+0.1% TritonX-100) for twice; Strep-HRP 50 ul/well was added and incubated at room temperature for 60 min; and then washed with 1xPBS (+0.1% TritonX-100) for twice. B 7.92 pIC50 12 nM IC50 US-9187430-B2. Poly (ADP-ribose) polymerase inhibitor (2015)
ChEMBL Inhibition of PARP1 (unknown origin) incubated for 10 mins using biotinylated NAD+ and activated DNA by colorimetric assay B 7.92 pIC50 11.9 nM IC50 J. Med. Chem. (2015) 58: 8683-8693 [PMID:26469301]
ChEMBL Inhibition of recombinant human PARP1 using histone as substrate after 1 hr in presence of NAD+ by ELISA B 7.96 pIC50 11 nM IC50 Eur J Med Chem (2018) 145: 389-403 [PMID:29335205]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.99 pIC50 10.23 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of GST-tagged recombinant human PARP-1 expressed in Escherichia coli after 30 mins by fluorescence-based assay B 8 pIC50 10 nM IC50 Bioorg. Med. Chem. Lett. (2014) 24: 462-466 [PMID:24388690]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 8 pIC50 10 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human PARP1 expressed in Escherichia coli incubated for 10 mins by colorimetric assay B 8.08 pIC50 8.39 nM IC50 Bioorg Med Chem (2019) 27: 3979-3997 [PMID:31401008]
ChEMBL Inhibition of recombinant human PARP-1 expressed in Escherichia coli BL21 (DE3) using sheared DNA as substrate in presence of biotinylated NAD after 1 hr by ELISA B 8.09 pIC50 8.1 nM IC50 Bioorg Med Chem (2017) 25: 4045-4054 [PMID:28622906]
ChEMBL Inhibition of PARP-1 (unknown origin) using biotinylated NAD+ as substrate after 60 mins by spectrophotometry B 8.15 pIC50 7 nM IC50 J. Med. Chem. (2014) 57: 5579-5601 [PMID:24922587]
ChEMBL Inhibition of human recombinant PARP-1 after 1 hr by ELISA B 8.22 pIC50 6 nM IC50 Bioorg. Med. Chem. Lett. (2014) 24: 2669-2673 [PMID:24815508]
ChEMBL Binding affinity to PARP1 in human OVCAR8 cells assessed as reduction in [125I]-KX1 binding by gamma counting analysis B 8.22 pIC50 6 nM IC50 J Med Chem (2018) 61: 5367-5379 [PMID:29856625]
ChEMBL Ex vivo inhibition of PARP1 in human SW620 cells assessed as amount of poly(ADP-ribose) by whole cell assay B 8.22 pIC50 6 nM IC50 J. Med. Chem. (2008) 51: 6581-6591 [PMID:18800822]
ChEMBL Inhibition of human PARP1 catalytic activity after 10 mins by ELISA B 8.22 pIC50 6 nM IC50 Bioorg. Med. Chem. Lett. (2013) 23: 4501-4505 [PMID:23850199]
ChEMBL Inhibition of recombinant human PARP1 using histone as substrate after 1 hr in presence of biotinylated NAD+ by ELISA B 8.3 pIC50 5.01 nM IC50 J Med Chem (2020) 63: 122-139 [PMID:31846325]
GtoPdb Assayed using PARP-1 isolated from HeLa cells, in a scintillation proximity ELISA. - 8.3 pIC50 5 nM IC50 J Med Chem (2008) 51: 6581-91 [PMID:18800822];
J Biomol Screen (2003) 8: 347-52 [PMID:12857389]
ChEMBL Inhibition of PARP1 by flashplate scintillation proximity assay B 8.3 pIC50 5 nM IC50 J. Med. Chem. (2008) 51: 6581-6591 [PMID:18800822]
ChEMBL Inhibition of PARP1 (unknown origin) B 8.3 pIC50 5 nM IC50 J. Med. Chem. (2015) 58: 3302-3314 [PMID:25761096]
ChEMBL Inhibition of PARP1 (unknown origin) B 8.3 pIC50 5 nM IC50 Bioorg Med Chem Lett (2016) 26: 4127-4132 [PMID:27353531]
ChEMBL Inhibition of recombinant human GST-fused PARP-1 expressed in Escherichia coli after 30 mins by fluorescence assay B 8.35 pIC50 4.5 nM IC50 Bioorg. Med. Chem. (2014) 22: 1089-1103 [PMID:24398383]
ChEMBL Inhibition of human recombinant PARP1 after 1 hr by ELISA B 8.4 pIC50 4 nM IC50 J. Med. Chem. (2013) 56: 2885-2903 [PMID:23473053]
ChEMBL Inhibition of PARP1 (unknown origin) by colorimetric assay B 8.44 pIC50 3.59 nM IC50 Bioorg Med Chem (2019) 27: 3979-3997 [PMID:31401008]
ChEMBL Inhibition of recombinant full length human N-terminal GST-tagged PARP1 expressed in baculovirus infected Sf9 cells using biotinylated substrate after 1 hr by UV/Vis spectrophotometric analysis B 8.44 pIC50 3.59 nM IC50 Bioorg Med Chem (2017) 25: 4100-4109 [PMID:28601509]
ChEMBL Inhibition of recombinant human PARP1 expressed in Escherichia coli BL21(DE3) using histone as substrate measured after 1 hr in presence of biotinylated NAD+ by ELISA B 8.68 pIC50 2.09 nM IC50 Eur J Med Chem (2017) 132: 26-41 [PMID:28340412]
ChEMBL Inhibition of human PARP1 using [3H]NAD as substrate after 1 min by microplate scintillation counting analysis B 8.71 pIC50 1.94 nM IC50 J. Med. Chem. (2016) 59: 335-357 [PMID:26652717]
ChEMBL Inhibition of recombinant human full length N-terminal GST- tagged PARP1 expressed in baculovirus infected sf9 cells using Colorimetric HRP as substrate incubated for 30 mins by UV/Vis spectrophotometer analysis B 8.74 pIC50 1.8 nM IC50 Eur J Med Chem (2019) 182: 111621-111621 [PMID:31442685]
ChEMBL Inhibition of PARP1 in human T98G cells incubated for 60 mins by immunofluorescence assay B 8.8 pIC50 1.6 nM IC50 J. Med. Chem. (2015) 58: 8683-8693 [PMID:26469301]
ChEMBL Inhibition of PARP1 in human G7 cells incubated for 60 mins by immunofluorescence assay B 8.8 pIC50 1.6 nM IC50 J. Med. Chem. (2015) 58: 8683-8693 [PMID:26469301]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP1 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 8.85 pIC50 1.4 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP1 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 8.86 pIC50 1.38 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human full-length N-terminal GST-tagged PARP1 expressed in baculovirus infected Sf9 insect cells using histone as substrate measured after 1 hr by horseradish peroxidase-coupled chemiluminescence assay B 8.92 pIC50 1.2 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
ChEMBL Inhibition of human full-length N-terminal GST-tagged PARP1 expressed in baculovirus infected Sf9 insect cells using histone as substrate measured after 1 hr by horseradish peroxidase-coupled chemiluminescence assay B 8.93 pIC50 1.18 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
ChEMBL Inhibition of full length human PARP1 expressed in Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 9 pIC50 1 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 9.05 pIC50 0.9 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of PARP1 in human Jurkat cells assessed as reduction of cell viability after 96 hrs by MTS assay B 4.8 pEC50 16000 nM EC50 Bioorg. Med. Chem. Lett. (2013) 23: 4501-4505 [PMID:23850199]
ChEMBL Inhibition of PARP1 in human Jurkat cells assessed as reduction of cell viability after 96 hrs by MTS assay in presence of 100 uM of temozolomide B 7.22 pEC50 60 nM EC50 Bioorg. Med. Chem. Lett. (2013) 23: 4501-4505 [PMID:23850199]
ChEMBL Inhibition of PARP-1 in human HeLa cells assessed as decrease of H2O2-induced PARylation after 3 hrs by fluorescence assay B 8.6 pEC50 2.5 nM EC50 Bioorg. Med. Chem. (2014) 22: 1089-1103 [PMID:24398383]
Poly [ADP-ribose] polymerase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2429708] [UniProtKB: Q53GL7]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP10 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.03 pIC50 9332.54 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP10 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.03 pIC50 9300 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP10 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.9 pIC50 1258.93 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP10 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.9 pIC50 1250 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 12 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2429709] [UniProtKB: Q9H0J9]
ChEMBL Inhibition of recombinant human His6-tagged PARP12 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 4.99 pIC50 10232.93 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP12 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5 pIC50 10050 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 15 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2176778] [UniProtKB: Q460N3]
ChEMBL Inhibition of recombinant human His6-tagged PARP15 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 4.75 pIC50 17782.79 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP15 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 4.75 pIC50 17600 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
poly(ADP-ribose) polymerase 2/Poly [ADP-ribose] polymerase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5366] [GtoPdb: 2772] [UniProtKB: Q9UGN5]
ChEMBL Binding affinity to recombinant human HisGST-tagged PARP-2 catalytic domain by surface plasmon resonance analysis B 9.55 pKd 0.28 nM Kd J. Med. Chem. (2015) 58: 6875-6898 [PMID:26222319]
ChEMBL Inhibition of recombinant human His6-tagged PARP2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.6 pIC50 251.19 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.6 pIC50 251 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP2 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.91 pIC50 12.3 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP2 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.91 pIC50 12.3 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human PARP2 using histone as substrate after 1.5 hr in presence of NAD+ by ELISA B 8.52 pIC50 3 nM IC50 Eur J Med Chem (2018) 145: 389-403 [PMID:29335205]
ChEMBL Inhibition of human PARP2 (2 to 583 residues) expressed in Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 8.52 pIC50 3 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
ChEMBL Inhibition of PARP2 (unknown origin) B 8.55 pIC50 2.81 nM IC50 Bioorg Med Chem (2019) 27: 3979-3997 [PMID:31401008]
ChEMBL Inhibition of recombinant human N-terminal GST-tagged PARP2 (2 to 583 residues) expressed in baculovirus infected Sf9 cells using biotinylated substrate after 1 hr by UV/Vis spectrophotometric analysis B 8.55 pIC50 2.81 nM IC50 Bioorg Med Chem (2017) 25: 4100-4109 [PMID:28601509]
ChEMBL Inhibition of recombinant human PARP-2 expressed in Escherichia coli BL21 (DE3) using sheared DNA as substrate in presence of biotinylated NAD after 1 hr by ELISA B 8.77 pIC50 1.7 nM IC50 Bioorg Med Chem (2017) 25: 4045-4054 [PMID:28622906]
GtoPdb Assayed using PARP-2 isolated from HeLa cells, in a scintillation proximity ELISA. - 9 pIC50 1 nM IC50 J Med Chem (2008) 51: 6581-91 [PMID:18800822];
J Biomol Screen (2003) 8: 347-52 [PMID:12857389]
ChEMBL Inhibition of recombinant human PARP2 using histone as substrate after 1 hr in presence of biotinylated NAD+ by ELISA B 9 pIC50 1 nM IC50 J Med Chem (2020) 63: 122-139 [PMID:31846325]
ChEMBL Inhibition of recombinant PARP2 by flashplate scintillation proximity assay B 9 pIC50 1 nM IC50 J. Med. Chem. (2008) 51: 6581-6591 [PMID:18800822]
ChEMBL Inhibition of human N-terminal GST-tagged PARP2 (2 to 583 residues) expressed in baculovirus infected Sf9 insect cells using histone as substrate measured after 1 hr by horseradish peroxidase-coupled chemiluminescence assay B 9.3 pIC50 0.5 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 9.35 pIC50 0.45 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of human N-terminal GST-tagged PARP2 (2 to 583 residues) expressed in baculovirus infected Sf9 insect cells using histone as substrate measured after 1 hr by horseradish peroxidase-coupled chemiluminescence assay B 9.4 pIC50 0.4 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
poly (ADP-ribose) polymerase 3/Poly [ADP-ribose] polymerase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5083] [GtoPdb: 2864] [UniProtKB: Q9Y6F1]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 6.49 pIC50 320 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of full length recombinant human His6-tagged PARP3 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.64 pIC50 230 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP3 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.64 pIC50 229.09 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of PARP3 (unknown origin) using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 7.34 pIC50 46 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
Poly [ADP-ribose] polymerase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6142] [UniProtKB: Q9UKK3]
ChEMBL Inhibition of recombinant human His6-tagged PARP4 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.39 pIC50 410 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP4 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.39 pIC50 407.38 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2380187] [UniProtKB: Q2NL67]
ChEMBL Inhibition of full length human PARP6 expressed in a Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 5.74 pIC50 1800 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
tankyrase/Tankyrase-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6164] [GtoPdb: 3108] [UniProtKB: O95271]
ChEMBL Inhibition of human recombinant TNKS in DLD1-Wnt-Luc cells after 20 to 22 hrs by luciferase reporter assay B 5.45 pIC50 3534 nM IC50 MedChemComm (2015) 6: 1687-1692
ChEMBL Inhibition of human TNKS1 (1001 to 1327 residues) expressed in Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 5.72 pIC50 1900 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
ChEMBL Inhibition of human N-terminal GST-tagged TNKS1 (1001 to 1327 residues) expressed in baculovirus infected sf9 cells using histone as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 5.74 pIC50 1800 nM IC50 J Med Chem (2018) 61: 5367-5379 [PMID:29856625]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 5.77 pIC50 1700 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
ChEMBL Inhibition of histidine-tagged recombinant tankyrase-1 by flashplate scintillation proximity assay B 5.82 pIC50 1500 nM IC50 J. Med. Chem. (2008) 51: 6581-6591 [PMID:18800822]
ChEMBL Inhibition of recombinant human TNKS1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.91 pIC50 1230.27 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human TNKS1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.91 pIC50 1230 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 7.98 pIC50 10.4 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)
tankyrase 2/Tankyrase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6154] [GtoPdb: 3109] [UniProtKB: Q9H2K2]
ChEMBL Inhibition of recombinant human TNKS2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.63 pIC50 2344.23 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human TNKS2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.63 pIC50 2340 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human TNKS2 (667 to 1166 residues) expressed in Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 5.77 pIC50 1700 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 5743-5747 [PMID:26546219]
ChEMBL Inhibition of human N-terminal GST-tagged TNKS2 (667 to 1166 residues) expressed in baculovirus infected sf9 cells using histone as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay B 5.92 pIC50 1200 nM IC50 J Med Chem (2018) 61: 5367-5379 [PMID:29856625]
ChEMBL Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested. B 8.28 pIC50 5.2 nM IC50 US-9255106-B2. Substituted [1,2,4]triazolo[4,3-a]pyrazines as PARP-1 inhibitors (2016)

ChEMBL data shown on this page come from version 28:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]