ChEMBL ligand: CHEMBL286080 (NCG-C00093856-01, SK-38393, SK-383933, SK&F-38393, SK&F-383933)
α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825] There should be some charts here, you may need to enable JavaScript!
D1 receptor/Dopamine D1 receptor in Human [ChEMBL: CHEMBL2056] [GtoPdb: 214] [UniProtKB: P21728] D1 receptor/Dopamine D1 receptor in Rat [ChEMBL: CHEMBL265] [GtoPdb: 214] [UniProtKB: P18901] There should be some charts here, you may need to enable JavaScript!
D2 receptor/Dopamine D2 receptor in Rat [ChEMBL: CHEMBL339] [GtoPdb: 215] [UniProtKB: P61169] There should be some charts here, you may need to enable JavaScript!
D5 receptor/Dopamine D5 receptor in Human [ChEMBL: CHEMBL1850] [GtoPdb: 218] [UniProtKB: P21918] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
CoV Replicase polyprotein 1ab/CoV RNA-dependent RNA polymerase/CoV Non-structural protein 15/CoV Non-structural protein 13/Replicase polyprotein 1ab in Severe acute respiratory syndrome coronavirus 2 [ChEMBL: CHEMBL4523582] [GtoPdb: 3125, 3139, 3206, 3261] [UniProtKB: P0DTD1] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825]
ChEMBL	Displacement of [3H]-UK14304 from recombinant human alpha2c adrenergic receptor expressed in CHOK1 cell membranes after 30 mins by scintillation counting analysis	B	7.08	pKi	83.18	nM	Ki	Bioorg Med Chem (2015) 23: 3991-3999 [PMID:25648685]
ChEMBL	Agonist activity at recombinant human alpha2c adrenergic receptor expressed in CHOK1 cells co-expressing Gqi5 assessed as induction of cytoplasmic calcium mobilization measured for 40 secs by fluorometric analysis	F	5.86	pEC50	1380.38	nM	EC50	Bioorg Med Chem (2015) 23: 3991-3999 [PMID:25648685]
D1 receptor/Dopamine D1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2056] [GtoPdb: 214] [UniProtKB: P21728]
ChEMBL	Displacement of [3H]SCH23390 from dopamine D1 receptor expressed in HEK293 cells by liquid scintillation counting	B	6.41	pKi	393	nM	Ki	Bioorg Med Chem (2009) 17: 4873-4880 [PMID:19559623]
ChEMBL	Displacement of [3H]SCH23390 from human dopamine D1 receptor expressed in HEK293 cell membranes by liquid scintillation counting based competition binding assay	B	6.41	pKi	393	nM	Ki	Eur J Med Chem (2014) 85: 16-26 [PMID:25076379]
ChEMBL	Agonist activity at dopamine D1 receptor	F	6.72	pKi	190	nM	Ki	Bioorg Med Chem (2009) 17: 4873-4880 [PMID:19559623]
GtoPdb	-	-	6.8	pKi	158	nM	Ki	Nature (1991) 350: 614-9 [PMID:1826762]; J Biol Chem (1994) 269: 27925-31 [PMID:7525564]
ChEMBL	Agonist activity at human dopamine D1 receptor expressed in CHOK1 cells assessed as assessed as increase in beta-arrestin-2 recruitment after 60 mins by luminescence assay	B	5	pEC50	>10000	nM	EC50	J Med Chem (2018) 61: 9841-9878 [PMID:29939744]
ChEMBL	Agonist activity at D1 receptor (unknown origin) expressed in CHOK1 cells assessed as increase in cAMP accumulation measured after 30 mins	F	6.18	pEC50	665.9	nM	EC50	Bioorg Med Chem (2019) 27: 2100-2111 [PMID:30981605]
ChEMBL	Formation of cAMP on Dopamine receptor D1 in vitro in carp retina	F	6.41	pEC50	386	nM	EC50	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Formation of cAMP on Dopamine receptor D1 in vitro in carp retina	F	6.41	pEC50	386	nM	EC50	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Agonist activity at dopamine D1 receptor expressed in HEK293 cells by by [35S]GTPgammaS binding assay	F	6.52	pEC50	300	nM	EC50	Bioorg Med Chem (2009) 17: 4873-4880 [PMID:19559623]
ChEMBL	[35S]GTPγS Binding Assay: For detecting the agonism action of the compounds, the [35S]GTPγS binding assay was performed at 30° C. for 40 mins in reaction buffer containing 50 mM Tris, pH 7.5, 5 mM MgCl2, 1 mM EDTA, 100 mM NaCl and 1 mM (DL)-dithiothreitol (DTT). The assay mixture (200 μL) contained 30 μg of membraneprotein, 0.1 nM [35S]GTPγS, and 40 μM guanosine triphosphate (GDP) with various concentration of the compound. The D1 receptor agonist SKF38393 and sntagonist SCH23390 were used for reference. Non specific binding was measured in the presence of 100 μM 50-guanylimidodiphosphate (Gpp(NH)p). The reaction was terminated by adding 3 mL of ice-cold washing buffer (50 mM Tris, pH 7.5, 5 mM MgCl2, 1 mM EDTA, and 100 mM NaCl) and was rapidly filtered with GF/C glass fiber filters (Whatman) and rinsed for three times.	B	6.61	pEC50	247.5	nM	EC50	US-9359372-B2. Hexahydrodibenzo[a,g]quinolizine compound, preparation method thereof, pharmaceutical composition and use thereof (2016)
ChEMBL	Agonist activity at D1 receptor (unknown origin) after 40 mins by [35S]GTP-gammaS binding assay	F	6.74	pEC50	180	nM	EC50	Bioorg Med Chem (2014) 22: 5838-5846 [PMID:25308766]
ChEMBL	Agonist activity at dopamine D1 receptor assessed as [35S]GTPgammaS binding in cell-based assay	F	6.77	pEC50	170	nM	EC50	Bioorg Med Chem (2012) 20: 4862-4871 [PMID:22748706]
ChEMBL	Agonist activity at dopamine D1 receptor (unknown origin) expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding by scintillation proximity assay	F	6.89	pEC50	130	nM	EC50	Bioorg Med Chem (2013) 21: 856-868 [PMID:23332346]
ChEMBL	Agonist activity at human dopamine D1 receptor expressed in CHOK1 cells assessed as reversal of Ro 20-1724 mediated decrease in cAMP accumulation after 60 mins by luminescence assay	F	8.96	pEC50	1.1	nM	EC50	J Med Chem (2018) 61: 9841-9878 [PMID:29939744]
D1 receptor/Dopamine D1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL265] [GtoPdb: 214] [UniProtKB: P18901]
ChEMBL	Binding affinity was determined by measuring the ability to displace [125I]SCH-23982 from Dopamine receptor D1 in rat caudate (in vitro)	B	7.19	pKi	64.1	nM	Ki	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Binding affinity was determined by measuring the ability to displace [125I]SCH-23982 from Dopamine receptor D1 in rat caudate (in vitro)	B	7.19	pKi	64	nM	Ki	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Displacement of [3H]-SCH- 23390 (0.3 nM) from dopamine receptor D1 in crude membrane fraction of rat brain corpus striatum	B	6.72	pIC50	190	nM	IC50	J Med Chem (1992) 35: 1466-1471 [PMID:1533424]
D2 receptor/Dopamine D2 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL339] [GtoPdb: 215] [UniProtKB: P61169]
ChEMBL	In vitro affinity at wild type Dopamine receptor D2 on C6 (glioma) cell membranes.	B	4.34	pKd	46000	nM	Kd	J Med Chem (2000) 43: 3005-3019 [PMID:10956209]
ChEMBL	In vitro affinity at mutant D2 receptor (S194A) in C6 (glioma) cell membranes.	B	4.41	pKd	39000	nM	Kd	J Med Chem (2000) 43: 3005-3019 [PMID:10956209]
ChEMBL	In vitro affinity at mutant D2 receptor (S194A) in C6 (glioma) cell membranes.	B	4.74	pKd	18000	nM	Kd	J Med Chem (2000) 43: 3005-3019 [PMID:10956209]
ChEMBL	Binding affinity was determined by measuring the ability to displace [125I]N-(p-aminophenethyl)-spiroperidol from Dopamine receptor D2 in rat caudate (in vitro)	B	5.16	pKi	6870	nM	Ki	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Binding affinity was determined by measuring the ability to displace [125I]N-(p-aminophenethyl)-spiroperidol from Dopamine receptor D2 in rat caudate (in vitro)	B	5.16	pKi	6870	nM	Ki	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Formation of cAMP on Dopamine receptor D2 in vitro in rat intermediate lobe	F	5	pEC50	>10000	nM	EC50	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
ChEMBL	Formation of cAMP on Dopamine receptor D2 in vitro in rat intermediate lobe	F	5	pEC50	>10000	nM	EC50	J Med Chem (1990) 33: 2948-2950 [PMID:1977907]
D5 receptor/Dopamine D5 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1850] [GtoPdb: 218] [UniProtKB: P21918]
ChEMBL	Displacement of [3H]SCH233930 from dopamine D5 receptor after 1.5 hrs by scintillation counting	B	6.91	pKi	124	nM	Ki	Bioorg Med Chem (2011) 19: 5756-5762 [PMID:21907583]
GtoPdb	-	-	7	pKi	-	-	-	Nature (1991) 350: 614-9 [PMID:1826762]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay	F	4.8	pIC50	15848.93	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
CoV Replicase polyprotein 1ab/CoV RNA-dependent RNA polymerase/CoV Non-structural protein 15/CoV Non-structural protein 13/Replicase polyprotein 1ab in Severe acute respiratory syndrome coronavirus 2 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523582] [GtoPdb: 3125, 3139, 3206, 3261] [UniProtKB: P0DTD1]
ChEMBL	SARS-CoV-2 3CL-Pro protease inhibition IC50 determined by FRET kind of response from peptide substrate	F	5.55	pIC50	2800	nM	IC50	Identification of inhibitors of SARS-Cov2 M-Pro enzymatic activity using a small molecule repurposing screen

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]