AQ-RA 741 [Ligand Id: 8584] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL43383
  • D1 receptor/Dopamine D1 receptor in Human [ChEMBL: CHEMBL2056] [GtoPdb: 214] [UniProtKB: P21728]
There should be some charts here, you may need to enable JavaScript!
  • D5 receptor/Dopamine D5 receptor in Human [ChEMBL: CHEMBL1850] [GtoPdb: 218] [UniProtKB: P21918]
There should be some charts here, you may need to enable JavaScript!
  • M1 receptor/Muscarinic acetylcholine receptor M1 in Human [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
  • M1 receptor/Muscarinic acetylcholine receptor M1 in Rat [ChEMBL: CHEMBL276] [GtoPdb: 13] [UniProtKB: P08482]
There should be some charts here, you may need to enable JavaScript!
  • M2 receptor/Muscarinic acetylcholine receptor M2 in Human [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172]
  • M2 receptor/Muscarinic acetylcholine receptor M2 in Rat [ChEMBL: CHEMBL309] [GtoPdb: 14] [UniProtKB: P10980]
There should be some charts here, you may need to enable JavaScript!
  • M3 receptor/Muscarinic acetylcholine receptor M3 in Human [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309]
  • M3 receptor/Muscarinic acetylcholine receptor M3 in Rat [ChEMBL: CHEMBL320] [GtoPdb: 15] [UniProtKB: P08483]
  • Muscarinic acetylcholine receptor M3 in Guinea pig [ChEMBL: CHEMBL5498] [UniProtKB: Q8VH26]
There should be some charts here, you may need to enable JavaScript!
  • M4 receptor/Muscarinic acetylcholine receptor M4 in Human [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173]
  • M4 receptor/Muscarinic acetylcholine receptor M4 in Rat [ChEMBL: CHEMBL317] [GtoPdb: 16] [UniProtKB: P08485]
There should be some charts here, you may need to enable JavaScript!
  • M5 receptor/Muscarinic acetylcholine receptor M5 in Human [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
D1 receptor/Dopamine D1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2056] [GtoPdb: 214] [UniProtKB: P21728]
ChEMBL PDSP Secondary Binding target: DRD1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 6.13 pKi 740.29 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
D5 receptor/Dopamine D5 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1850] [GtoPdb: 218] [UniProtKB: P21918]
ChEMBL PDSP Secondary Binding target: DRD5 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 5.73 pKi 1845.44 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
ChEMBL PDSP Secondary Binding target: CHRM1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 6.59 pKi 257.66 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
ChEMBL Binding affinity to muscarinic M1 receptor (unknown origin) expressed in CHO cells B 7.3 pKi 50.12 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
GtoPdb - - 7.5 pKi - - - J Pharmacol Exp Ther (1991) 256: 727-33 [PMID:1994002];
Eur J Pharmacol (1998) 349: 285-92 [PMID:9671109]
ChEMBL Displacement of [3H]-NMS from human muscarinic M1 receptor expressed in stable CHO-K1 cells by radioligand binding assay B 7.54 pKi 28.84 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
M1 receptor/Muscarinic acetylcholine receptor M1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL276] [GtoPdb: 13] [UniProtKB: P08482]
ChEMBL Tested for affinity constant against M1 muscarinic receptor in rat cortex using [3H]pirenzepine B 6.98 pKi 104.71 nM Ki J Med Chem (1994) 37: 3363-3372 [PMID:7932564]
ChEMBL Affinity constant measured against M1 muscarinic receptor in rat cortex B 7.6 pKi 25.12 nM Ki J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
M2 receptor/Muscarinic acetylcholine receptor M2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172]
ChEMBL Antagonistic activity against M2 muscarinic receptor in guinea pig left atrium derived by plotting log(DR - 1) vs log[antagonist] F 8.03 pKd 9.33 nM Kd J Med Chem (1994) 37: 3363-3372 [PMID:7932564]
ChEMBL Antagonistic activity measured in isolated guinea pig left atrium (M2 receptor) F 8.5 pKd 3.16 nM Kd J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
ChEMBL PDSP Secondary Binding target: CHRM2 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 7.84 pKi 14.46 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
ChEMBL Binding affinity to muscarinic M2 receptor (unknown origin) expressed in CHO cells B 8.3 pKi 5.01 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL Displacement of [3H]-NMS from human muscarinic M2 receptor expressed in stable CHO-K1 cells by radioligand binding assay B 8.37 pKi 4.27 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
GtoPdb - - 8.4 pKi - - - J Pharmacol Exp Ther (1991) 256: 727-33 [PMID:1994002];
Eur J Pharmacol (1998) 349: 285-92 [PMID:9671109]
ChEMBL GPCR PRESTO-Tango dose-response in antagonist mode with target: CHRM2 F 6.03 pIC50 931.3 nM IC50 EUbOPEN Chemogenomics Library - GPCR Dose-Respose
M2 receptor/Muscarinic acetylcholine receptor M2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL309] [GtoPdb: 14] [UniProtKB: P10980]
ChEMBL Affinity constant measured against M2 muscarinic receptor in rat heart B 8.3 pKi 5.01 nM Ki J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
ChEMBL Tested for affinity constant against M2 muscarinic receptor rat heart using [3H]-NMS B 8.49 pKi 3.24 nM Ki J Med Chem (1994) 37: 3363-3372 [PMID:7932564]
M3 receptor/Muscarinic acetylcholine receptor M3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309]
ChEMBL PDSP Secondary Binding target: CHRM3 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 6.82 pKi 152.23 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
ChEMBL Binding affinity to muscarinic M3 receptor (unknown origin) expressed in CHO cells B 7 pKi 100 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL Displacement of [3H]-NMS from human muscarinic M3 receptor expressed in stable CHO-K1 cells by radioligand binding assay B 7.2 pKi 63.1 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
GtoPdb - - 7.3 pKi - - - J Pharmacol Exp Ther (1991) 256: 727-33 [PMID:1994002];
Eur J Pharmacol (1998) 349: 285-92 [PMID:9671109]
M3 receptor/Muscarinic acetylcholine receptor M3 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL320] [GtoPdb: 15] [UniProtKB: P08483]
ChEMBL Tested for affinity constant against M3 muscarinic receptor in rat submaxillary gland using [3H]NMS B 6.6 pKi 251.19 nM Ki J Med Chem (1994) 37: 3363-3372 [PMID:7932564]
ChEMBL Affinity constant measured against M3 muscarinic receptor in rat submaxillary gland B 6.8 pKi 158.49 nM Ki J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
Muscarinic acetylcholine receptor M3 in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5498] [UniProtKB: Q8VH26]
ChEMBL Antagonistic activity measured in isolated guinea pig left ileum (M3 receptor) F 6.6 pKd 251.19 nM Kd J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
ChEMBL Antagonistic activity against M3 muscarinic receptor in guinea pig left ileum derived by plotting log(DR - 1) vs log[antagonist] F 7 pKd 100 nM Kd J Med Chem (1994) 37: 3363-3372 [PMID:7932564]
M4 receptor/Muscarinic acetylcholine receptor M4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173]
ChEMBL PDSP Secondary Binding target: CHRM4 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 7.87 pKi 13.59 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
ChEMBL Binding affinity to muscarinic M4 receptor (unknown origin) expressed in CHO cells B 8.1 pKi 7.94 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL Displacement of [3H]-NMS from human muscarinic M4 receptor expressed in stable CHO-K1 cells by radioligand binding assay B 8.19 pKi 6.46 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
GtoPdb - - 8.2 pKi - - - J Pharmacol Exp Ther (1991) 256: 727-33 [PMID:1994002];
Eur J Pharmacol (1998) 349: 285-92 [PMID:9671109]
M4 receptor/Muscarinic acetylcholine receptor M4 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL317] [GtoPdb: 16] [UniProtKB: P08485]
ChEMBL Affinity constant measured against M4 muscarinic receptor rat NG108-15 cells B 8 pKi 10 nM Ki J Med Chem (1993) 36: 3734-3737 [PMID:8246244]
M5 receptor/Muscarinic acetylcholine receptor M5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912]
ChEMBL Binding affinity to muscarinic M5 receptor (unknown origin) expressed in CHO cells B 5.9 pKi 1258.93 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
ChEMBL PDSP Secondary Binding target: CHRM5 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 6.02 pKi 944.28 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding
ChEMBL Displacement of [3H]-NMS from human muscarinic M5 receptor expressed in stable CHO-K1 cells by radioligand binding assay B 6.08 pKi 831.76 nM Ki J Med Chem (2019) 62: 5358-5369 [PMID:31074983]
GtoPdb - - 7.8 pKi - - - J Pharmacol Exp Ther (1991) 256: 727-33 [PMID:1994002];
Eur J Pharmacol (1998) 349: 285-92 [PMID:9671109]
σ2/Sigma intracellular receptor 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105907] [GtoPdb: 2553] [UniProtKB: Q5BJF2]
ChEMBL PDSP Secondary Binding target: TMEM97 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity. B 5.88 pKi 1308.88 nM Ki EUbOPEN Chemogenomics Library - PDSP Secondary Binding

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]