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rizavasertib   Click here for help

GtoPdb Ligand ID: 8204

Synonyms: A 443654 | A-443654 | A443654
PDB Ligand
Compound class: Synthetic organic
Comment: A-443654 is an ATP-competitive inhibitor of all three Akt (protein kinase B) subtypes [3], but causes paradixical hyperphosphorylation of two regulatory amino acid residues (Thr308 and Ser473) of the Akt protein [2,4]. A-443654 exhibits modest selectivity for Akt, compared to other AGC family kinases, such as PKA and PKC [3].
The chemical structure of A-443654 is identical to that for the INN rizavasertib (proposed list 132, Feb. 2025).
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Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

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View interactive charts of activity data across species

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2D Structure
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2D Structure

An image of the ligand's 2D structure. For small molecules with SMILES these are drawn using the NCI/CADD Chemical Identifier Resolver. Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. For other types of ligands, e.g. longer nucleotides and peptides, a manually drawn representation of the molecule may be provided.

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Physico-chemical Properties
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Physico-chemical Properties

Calculated molecular properties are available for small molecules and natural products (not peptides). Properties were generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/). All properties were selected to enable the prediction of the Lipinski Rule-of-Five profile or ‘druglikeness’ for each ligand. For more info on each category see the help pages.

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Hydrogen bond acceptors 3
Hydrogen bond donors 3
Rotatable bonds 6
Topological polar surface area 92.61
Molecular weight 397.19
XLogP 4.17
No. Lipinski's rules broken 0

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
SMILES / InChI / InChIKey
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SMILES / InChI / InChIKey

SMILES (Simplified Molecular Input Line Entry Specification) A specification for unambiguously describing the structure of chemical molecules using short ASCII strings. Canonical SMILES specify a unique representation of the 2D structure without chiral or isotopic specifications. Isomeric SMILES include chiral specification and isotopes.

Standard InChI (IUPAC International Chemical Identifier) and InChIKey InChI is a non-proprietary, standard, textual identifier for chemical substances designed to facilitate linking of information and database searching. An InChIKey is a simplified version of a full InChI, designed for easier web searching.

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)

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Canonical SMILES NC(Cc1c[nH]c2c1cccc2)COc1cncc(c1)c1ccc2c(c1)c(C)n[nH]2
Isomeric SMILES N[C@@H](Cc1c[nH]c2c1cccc2)COc1cncc(c1)c1ccc2c(c1)c(C)n[nH]2
InChI InChI=1S/C24H23N5O/c1-15-22-10-16(6-7-24(22)29-28-15)17-9-20(13-26-11-17)30-14-19(25)8-18-12-27-23-5-3-2-4-21(18)23/h2-7,9-13,19,27H,8,14,25H2,1H3,(H,28,29)/t19-/m0/s1
InChI Key YWTBGJGMTBHQTM-IBGZPJMESA-N

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
Bioactivity Comments
In a selectivity profile screen of 220 kinases, A-443654 is reported to inhibit 47 additional kinases by >90% using 1μM compound [4]. These off-targets include CAMK2D, MRCK alpha and beta, CLK1, -2 and -3, DYRK1A, -1B and -3, GRK7, GSK3β, the MAP kinases GCK, HGK and KHS1, MINK1 and others [4]. Claimed to reduce α-synuclein accumulation (pathological features of α-synucleinopathies, including Parkinson's disease) via AKT-mediated regulation of α-synuclein gene (SNCA) expression [1].
Selectivity at enzymes
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
AKT serine/threonine kinase 1 Primary target of this compound Hs Inhibitor Inhibition 9.8 pKi - 3
⤷ pKi 9.8 (Ki 1.6x10-10 M) [3]
AKT serine/threonine kinase 2 Hs Inhibitor Inhibition ~9.8 pKi - 3
⤷ pKi ~9.8 (Ki ~1.6x10-10 M) [3]
AKT serine/threonine kinase 3 Hs Inhibitor Inhibition ~9.8 pKi - 3
⤷ pKi ~9.8 (Ki ~1.6x10-10 M) [3]
CDC42 binding protein kinase beta Hs Inhibitor Inhibition - - - 4
⤷ [4]
Description: Measured as % inhibition using 1μM compound.
phosphorylase kinase catalytic subunit gamma 1 Hs Inhibitor Inhibition - - - 4
⤷ [4]
Description: Measured as % inhibition using 1μM compound.