D<sub>4</sub> receptor | Dopamine receptors | IUPHAR/BPS Guide to PHARMACOLOGY

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D4 receptor

Target not currently curated in GtoImmuPdb

Target id: 217

Nomenclature: D4 receptor

Family: Dopamine receptors

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

Contents:

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 467 11p15.5 DRD4 dopamine receptor D4 74
Mouse 7 387 7 F5 Drd4 dopamine receptor D4 41
Rat 7 387 1q41 Drd4 dopamine receptor D4 17
Previous and Unofficial Names
d(2C) dopamine receptor | dopamine receptor 4 | D4R
Database Links
Specialist databases
GPCRDB drd4_human (Hs), drd4_mouse (Mm), drd4_rat (Rn)
Other databases
ChEMBL Target CHEMBL219 (Hs), CHEMBL2574 (Mm), CHEMBL3361 (Rn)
DrugBank Target P21917 (Hs)
Ensembl Gene ENSG00000069696 (Hs), ENSMUSG00000025496 (Mm), ENSRNOG00000017927 (Rn)
Entrez Gene 1815 (Hs), 13491 (Mm), 25432 (Rn)
Human Protein Atlas ENSG00000069696 (Hs)
KEGG Gene hsa:1815 (Hs), mmu:13491 (Mm), rno:25432 (Rn)
OMIM 126452 (Hs)
Pharos P21917 (Hs)
RefSeq Nucleotide NM_000797 (Hs), NM_007878 (Mm), NM_012944 (Rn)
RefSeq Protein NP_000788 (Hs), NP_031904 (Mm), NP_037076 (Rn)
UniProtKB P21917 (Hs), P51436 (Mm), P30729 (Rn)
Wikipedia DRD4 (Hs)
Selected 3D Structures
Image of receptor 3D structure from RCSB PDB
Description:  Structure of the human D4 Dopamine receptor in complex with Nemonapride
PDB Id:  5WIU
Ligand:  nemonapride
Resolution:  1.96Å
Species:  Human
References:  77
Image of receptor 3D structure from RCSB PDB
Description:  Structure of the sodium-bound human D4 Dopamine receptor in complex with Nemonapride
PDB Id:  5WIV
Ligand:  nemonapride
Resolution:  2.14Å
Species:  Human
References:  77
Natural/Endogenous Ligands
dopamine

Download all structure-activity data for this target as a CSV file

Agonists
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
CP-226269 Rn Partial agonist 9.4 pKi 26
⤷ pKi 9.4 [26]
FAUC213 Rn Partial agonist 9.0 pKi 26
⤷ pKi 9.0 [26]
PD168,077 Rn Partial agonist 8.8 pKi 26
⤷ pKi 8.8 (Ki 1.58x10-9 M) [26]
apomorphine Hs Partial agonist 8.4 pKi 36
⤷ pKi 8.4 [36]
lisuride Hs Partial agonist 8.3 pKi 36
⤷ pKi 8.3 [36]
roxindole Hs Partial agonist 8.2 pKi 36
⤷ pKi 8.2 [36]
A412997 Rn Agonist 8.1 pKi 37
⤷ pKi 8.1 (Ki 7.9x10-9 M) [37]
A412997 Hs Full agonist 8.1 pKi 37
⤷ pKi 8.1 (Ki 7.9x10-9 M) [37]
WAY-100635 Hs Full agonist 7.8 pKi 1
⤷ pKi 7.8 (Ki 1.6x10-8 M) [1]
dopamine Hs Full agonist 7.6 pKi 74
⤷ pKi 7.6 [74]
quinpirole Hs Full agonist 7.5 pKi 36,44,74
⤷ pKi 7.5 [36,44,74]
dopamine Rn Full agonist 7.4 pKi 53
⤷ pKi 7.4 [53]
cabergoline Hs Partial agonist 7.3 pKi 36
⤷ pKi 7.3 [36]
rotigotine Hs Agonist 7.3 pKi 76
⤷ pKi 7.3 (Ki 5.5x10-8 M) [76]
pergolide Hs Partial agonist 7.2 pKi 36
⤷ pKi 7.2 [36]
fenoldopam Hs Full agonist 6.5 pKi 74
⤷ pKi 6.5 [74]
ABT-670 Hs Full agonist 5.8 pKi 42
⤷ pKi 5.8 (Ki 1.445x10-6 M) [42]
PF-592379 Hs Agonist 5.4 pIC50 12
⤷ pIC50 5.4 (IC50 4.165x10-6 M) [12]
View species-specific agonist tables
Antagonists
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
nemonapride Hs Antagonist 10.0 pKd 59
⤷ pKd 10.0 [59]
[125I]L750667 Hs Antagonist 9.8 pKd 44
⤷ pKd 9.8 (Kd 1.6x10-10 M) [44]
[3H]spiperone Hs Antagonist 9.5 pKd 24,74
⤷ pKd 9.5 (Kd 3x10-10 M) [24,74]
[3H]NGD941 Hs Antagonist 8.3 pKd 47
⤷ pKd 8.3 (Kd 5x10-9 M) [47]
benperidol Hs Antagonist 10.2 pKi 60
⤷ pKi 10.2 (Ki 6.6x10-11 M) [60]
perospirone Hs Antagonist 10.1 pKi 60
⤷ pKi 10.1 (Ki 9x10-11 M) [60]
L-750,667 Rn Antagonist 10.0 pKi 53
⤷ pKi 10.0 [53]
RBI257 Rn Antagonist 9.6 pKi 26
⤷ pKi 9.6 [26]
[3H]N-methylspiperone Rn Antagonist 9.5 pKi 53
⤷ pKi 9.5 [53]
CP-293019 Rn Antagonist 9.5 pKi 26
⤷ pKi 9.5 [26]
NGD 94-1 Rn Antagonist 9.5 pKi 26
⤷ pKi 9.5 [26]
L745870 Hs Antagonist 9.4 pKi 28
⤷ pKi 9.4 (Ki 3.9x10-10 M) [28]
spiperone Hs Antagonist 9.3 pKi 14
⤷ pKi 9.3 [14]
FAUC213 Rn Antagonist 9.0 pKi 26
⤷ pKi 9.0 [26]
sonepiprazole Hs Antagonist 8.9 pKi 54
⤷ pKi 8.9 (Ki 1.4x10-9 M) [54]
U101958 Hs Antagonist 8.9 pKi 54
⤷ pKi 8.9 (Ki 1.4x10-9 M) [54]
A-381393 Hs Antagonist 8.8 pKi 39
⤷ pKi 8.8 (Ki 1.5x10-9 M) [39]
haloperidol Hs Antagonist 8.7 – 8.8 pKi 29,61,73
⤷ pKi 8.7 – 8.8 [29,61,73]
sonepiprazole Rn Antagonist 8.7 pKi 26
⤷ pKi 8.7 [26]
L741742 Hs Antagonist 8.5 pKi 51
⤷ pKi 8.5 (Ki 3.16x10-9 M) [51]
sertindole Hs Antagonist 7.8 – 9.1 pKi 7,57-58,60
⤷ pKi 7.8 – 9.1 (Ki 1.6x10-8 – 8.5x10-10 M) [7,57-58,60]
loxapine Hs Antagonist 8.1 pKi 58
⤷ pKi 8.1 (Ki 7.8x10-9 M) [58]
terguride Hs Antagonist 8.1 pKi 36
⤷ pKi 8.1 [36]
chlorpromazine Hs Antagonist 7.8 pKi 29
⤷ pKi 7.8 [29]
Ro 10-4548 Rn Antagonist 7.8 pKi 26
⤷ pKi 7.8 [26]
clozapine Hs Antagonist 7.5 pKi 29
⤷ pKi 7.5 [29]
ML398 Hs Antagonist 7.4 pKi 6
⤷ pKi 7.4 (Ki 3.6x10-8 M) [6]
zotepine Hs Antagonist 7.4 pKi 56
⤷ pKi 7.4 (Ki 3.9x10-8 M) [56]
trifluoperazine Hs Antagonist 7.4 pKi 58
⤷ pKi 7.4 (Ki 4.4x10-8 M) [58]
aripiprazole Rn Antagonist 7.3 pKi 53
⤷ pKi 7.3 [53]
eticlopride Hs Antagonist 7.0 pKi 14
⤷ pKi 7.0 [14]
(-)-sulpiride Hs Antagonist 5.7 – 7.7 pKi 44,70
⤷ pKi 5.7 – 7.7 (Ki 1.9x10-6 – 2x10-8 M) [44,70]
piribedil Hs Antagonist 6.5 pKi 36
⤷ pKi 6.5 [36]
bromocriptine Hs Antagonist 6.4 pKi 36
⤷ pKi 6.4 [36]
nafadotride Rn Antagonist 6.4 pKi 53
⤷ pKi 6.4 [53]
(+)-butaclamol Hs Antagonist 6.3 pKi 44
⤷ pKi 6.3 [44]
prochlorperazine Hs Antagonist 6.1 pKi 3
⤷ pKi 6.1 (Ki 8.1x10-7 M) [3]
raclopride Rn Antagonist 5.7 pKi 53
⤷ pKi 5.7 [53]
(+)-sulpiride Hs Antagonist 5.7 pKi 44
⤷ pKi 5.7 (Ki 2.2x10-6 M) [44]
View species-specific antagonist tables
Immuno Process Associations
Immuno Process:  Antigen presentation
GO Annotations:  Associated to 1 GO processes, IEA only
click arrow to show/hide IEA associations
GO:0099149 regulation of postsynaptic neurotransmitter receptor internalization IEA
Primary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family Adenylate cyclase inhibition
References:  45
Tissue Distribution
Brain: Occipital lobe, cerebellum, hippocampus, middle frontal gyrus of temporal lobe, middle frontal gyrus of frontal lobe, cingulate gyrus of frontal lobe, amygdala > superior temporal gyrus of temporal lobe, superior frontal gyrus of frontal lobe, thalamus, septal nuclei, hypothalamus > substantia nigra, caudate nucleus, globus pallidus, superior parietal lobe.
Species:  Human
Technique:  RT-PCR
References:  38
Brain: Pre-frontal cortex, temporal neocortex > occipital cortex.
Species:  Human
Technique:  in situ hybridization.
References:  34
Aortic endothelium, umbilical vein endothelium.
Species:  Human
Technique:  RT-PCR
References:  80
Pulmonary artery.
Species:  Human
Technique:  Western Blot and Immunohistochemistry.
References:  48
Brain (especially cerebellum), eye, adrenal gland, testes, superior cervical ganglion, submaxillary gland, pancreas.
Species:  Mouse
Technique:  RT-PCR
References:  67
Retina, photoreceptor cell layer and inner nuclear and ganglion cell layers.
Species:  Mouse
Technique:  RT-PCR and in situ hybridization.
References:  11
Motor, somatosensory, visual, auditory, temporal association, cingulate, retrosplenial and granular insular cortices.
Species:  Rat
Technique:  Immunohistochemistry.
References:  49
Kidney: Cortical collecting duct.
Species:  Rat
Technique:  RT-PCR
References:  65
Striatum, substantia nigra pars reticulata, entopeduncular nucleus, globus pallidus.
Species:  Rat
Technique:  Immunohistochemistry.
References:  50
Nucleus accumbens shell.
Species:  Rat
Technique:  immunocytochemistry.
References:  68
Pineal gland.
Species:  Rat
Technique:  RT-PCR.
References:  25
Kidney: Cortex > inner medulla.
Species:  Rat
Technique:  Western Blot and Immunocytochemistry.
References:  63
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Measurement of cAMP levels in HEK 293 cells transfected with the human D4 receptor.
Species:  Human
Tissue:  HEK 293 cells.
Response measured:  Inhibition of cAMP accumulation.
References:  8,18,32
Measurement of cAMP levels in CHO cells transfected with the human D4 receptor.
Species:  Human
Tissue:  CHO cells.
Response measured:  Inhibition of cAMP accumulation.
References:  2,10,30,43,62,69
Measurement of cAMP levels in COS-7 cells transfected with the human D4 receptor.
Species:  Human
Tissue:  COS-7 cells.
Response measured:  Inhibition of cAMP accumulation.
References:  2
Measurement of cAMP levels in Chinese hamster lung fibroblast CCL39 cells transfected with the rat D4 receptor.
Species:  Rat
Tissue:  Chinese hamster lung fibroblast CCL39 cells.
Response measured:  Inhibition of cAMP accumulation.
References:  20
Measurement of high-voltage Ca2+ current in cultured granule cells from neonatal rat cerebellum endogenously expressing the D4 receptor.
Species:  Rat
Tissue:  Granule cells.
Response measured:  Inhibition of L-type Ca2+ current.
References:  35
Measurement of mitogenesis in CHO cells transfected with the human D4 receptor.
Species:  Human
Tissue:  CHO cells.
Response measured:  Mitogenesis.
References:  54
Measurement of Cl- currents in Xenopus oocytes transfected with murine D2L and D2S receptors.
Species:  Mouse
Tissue:  Xenopus oocytes.
Response measured:  Stimulation of Cl- influx.
References:  64
Measurement of inwardly rectifying K+ currents in Xenopus oocytes transfected with the human D4 receptor.
Species:  Human
Tissue:  Xenopus oocytes.
Response measured:  Activation of GIRK1 channels.
References:  46,78
Recruitment of beta-arrestin.
Species:  Human
Tissue:  Transfected CHO and HEK293 cells.
Response measured: 
References:  5,27,33
Physiological Functions
Modulation of the dark level of light-sensitive cAMP in photoreceptors.
Species:  Mouse
Tissue:  Retina.
References:  11
Regulation of electrolyte and water transport.
Species:  Rat
Tissue:  Kidney.
References:  63,65
Modulation of von Willebrand factor secretion in endothelial cells.
Species:  Human
Tissue:  Endothelium.
References:  80
Modulation of gastric acid secretion.
Species:  Rat
Tissue:  In vivo.
References:  21
Modulation of angiotensin II type 1 receptor expression.
Species:  Rat
Tissue:  Kidney.
References:  9
Modulation of neuronal electrophysiological properties.
Species:  Mouse
Tissue:  Prefrontal cortex.
References:  81
Modulation of AMPA receptor trafficking and functions.
Species:  Rat
Tissue:  Prefrontal cortex.
References:  79
Modulation of GABAA receptor functions.
Species:  Rat
Tissue:  Prefrontal cortex primary neurons.
References:  23
Physiological Consequences of Altering Gene Expression
D4 receptor knockout mice exhibit an increased sensitivity to ethanol, cocaine and methamphetamine.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  52
D4 receptor knockout mice exhibit a decrease in novelty-related exploration.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  13
D4 receptor knockout mice exhibit increased avoidance behaviour to unconditioned stimuli.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  16
D4 receptor knockout mice exhibit dysfunctional light-evoked regulation of cAMP in photoreceptors and abnormal retinal adaptation.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  40
D4 receptor knockout mice exhibit increased co-ordination.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells.
References:  52
D4 receptor knockout mice exhibit reduced longevity.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells and genotype polymorphism.
References:  22
D4 receptor knockout mice exhibit an increase in blood pressure.
Species:  Mouse
Tissue: 
Technique:  Gene targeting in embryonic stem cells and genotype polymorphism.
References:  4
D4 receptor knockout mice exhibit an increase in glutamate extracellular levels.
Species:  Mouse
Tissue:  Striatum.
Technique:  Gene targeting in embryonic stem cells and genotype polymorphism.
References:  71
D4 receptor knockout mice exhibit a decrease in dopamine release.
Species:  Mouse
Tissue:  Striatum.
Technique:  Gene targeting in embryonic stem cells and genotype polymorphism.
References:  72
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Drd4tm1(IL2RA)Tao Drd4tm1(IL2RA)Tao/Drd4tm1(IL2RA)Tao
involves: C57BL/6J		 MGI:94926  MP:0003463 abnormal single cell response PMID: 14684868 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0001417 decreased exploration in new environment PMID: 10531457 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0002757 decreased vertical activity PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0009754 enhanced behavioral response to cocaine PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0003858 enhanced coordination PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0002629 hyperactivity elicited by ethanol administration PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0001402 hypoactivity PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0009750 impaired behavioral response to addictive substance PMID: 9323127 
Drd4tm1Dkg Drd4tm1Dkg/Drd4tm1Dkg
involves: 129P2/OlaHsd * C57BL/6J		 MGI:94926  MP:0001906 increased dopamine level PMID: 9323127 
Biologically Significant Variants
Type:  Repeat polymorphism of Exon III
Species:  Human
Description:  DRD4 7-repeat allele may increase likelihood of ADHD.
References:  15,66
General Comments
Human and primate receptors have a variable number (2-10) of imperfect 48 bp repeats in exon 3 [31,75]. This leads to a highly variable length and amino acid composition of the putative third cytoplasmic loop, apparently not associated with any marked variation in pharmacology. In humans the D4.2, D4.4 and D4.7 variants are the predominant variants [55].

Most D4 receptor-antagonists display agonistic activities in some heterologous expression systems [18-19].

References

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1. Al Hussainy R, Verbeek J, van der Born D, Braker AH, Leysen JE, Knol RJ, Booij J, Herscheid JK. (2011) Design, synthesis, radiolabeling, and in vitro and in vivo evaluation of bridgehead iodinated analogues of N-{2-[4-(2-methoxyphenyl)piperazin-1-yl]ethyl}-N-(pyridin-2-yl)cyclohexanecarboxamide (WAY-100635) as potential SPECT ligands for the 5-HT1A receptor. J. Med. Chem., 54 (10): 3480-91. [PMID:21520940]

2. Asghari V, Sanyal S, Buchwaldt S, Paterson A, Jovanovic V, Van Tol HH. (1995) Modulation of intracellular cyclic AMP levels by different human dopamine D4 receptor variants. J. Neurochem., 65 (3): 1157-65. [PMID:7643093]

3. Auerbach SS, DrugMatrix® and ToxFX® Coordinator National Toxicology Program. National Toxicology Program: Dept of Health and Human Services. Accessed on 02/05/2014. Modified on 02/05/2014. DrugMatrix, https://ntp.niehs.nih.gov/drugmatrix/index.html

4. Bek MJ, Wang X, Asico LD, Jones JE, Zheng S, Li X, Eisner GM, Grandy DK, Carey RM, Soares-da-Silva P et al.. (2006) Angiotensin-II type 1 receptor-mediated hypertension in D4 dopamine receptor-deficient mice. Hypertension, 47 (2): 288-95. [PMID:16380537]

5. Bergman J, Roof RA, Furman CA, Conroy JL, Mello NK, Sibley DR, Skolnick P. (2013) Modification of cocaine self-administration by buspirone (buspar®): potential involvement of D3 and D4 dopamine receptors. Int. J. Neuropsychopharmacol., 16 (2): 445-58. [PMID:22827916]

6. Berry CB, Bubser M, Jones CK, Hayes JP, Wepy JA, Locuson CW, Daniels JS, Lindsley CW, Hopkins CR. (2014) Discovery and Characterization of ML398, a Potent and Selective Antagonist of the D4 Receptor with in Vivo Activity. ACS Med Chem Lett, 5 (9): 1060-4. [PMID:25221667]

7. Burstein ES, Ma J, Wong S, Gao Y, Pham E, Knapp AE, Nash NR, Olsson R, Davis RE, Hacksell U et al.. (2005) Intrinsic efficacy of antipsychotics at human D2, D3, and D4 dopamine receptors: identification of the clozapine metabolite N-desmethylclozapine as a D2/D3 partial agonist. J. Pharmacol. Exp. Ther., 315 (3): 1278-87. [PMID:16135699]

8. Chemel BR, Roth BL, Armbruster B, Watts VJ, Nichols DE. (2006) WAY-100635 is a potent dopamine D4 receptor agonist. Psychopharmacology (Berl.), 188 (2): 244-51. [PMID:16915381]

9. Chen K, Deng K, Wang X, Wang Z, Zheng S, Ren H, He D, Han Y, Asico LD, Jose PA et al.. (2015) Activation of D4 dopamine receptor decreases angiotensin II type 1 receptor expression in rat renal proximal tubule cells. Hypertension, 65 (1): 153-60. [PMID:25368031]

10. Chio CL, Drong RF, Riley DT, Gill GS, Slightom JL, Huff RM. (1994) D4 dopamine receptor-mediated signaling events determined in transfected Chinese hamster ovary cells. J. Biol. Chem., 269 (16): 11813-9. [PMID:7512953]

11. Cohen AI, Todd RD, Harmon S, O'Malley KL. (1992) Photoreceptors of mouse retinas possess D4 receptors coupled to adenylate cyclase. Proc. Natl. Acad. Sci. U.S.A., 89 (24): 12093-7. [PMID:1334557]

12. Collins GT, Butler P, Wayman C, Ratcliffe S, Gupta P, Oberhofer G, Caine SB. (2012) Lack of abuse potential in a highly selective dopamine D3 agonist, PF-592,379, in drug self-administration and drug discrimination in rats. Behav Pharmacol, 23 (3): 280-91. [PMID:22470105]

13. Dulawa SC, Grandy DK, Low MJ, Paulus MP, Geyer MA. (1999) Dopamine D4 receptor-knock-out mice exhibit reduced exploration of novel stimuli. J. Neurosci., 19 (21): 9550-6. [PMID:10531457]

14. Durcan MJ, Rigdon GC, Norman MH, Morgan PF. (1995) Is clozapine selective for the dopamine D4 receptor?. Life Sci., 57 (18): PL275-83. [PMID:7475902]

15. El-Faddagh M, Laucht M, Maras A, Vöhringer L, Schmidt MH. (2004) Association of dopamine D4 receptor (DRD4) gene with attention-deficit/hyperactivity disorder (ADHD) in a high-risk community sample: a longitudinal study from birth to 11 years of age. J Neural Transm (Vienna), 111 (7): 883-9. [PMID:15206004]

16. Falzone TL, Gelman DM, Young JI, Grandy DK, Low MJ, Rubinstein M. (2002) Absence of dopamine D4 receptors results in enhanced reactivity to unconditioned, but not conditioned, fear. Eur. J. Neurosci., 15 (1): 158-64. [PMID:11860516]

17. Fishburn CS, Carmon S, Fuchs S. (1995) Molecular cloning and characterisation of the gene encoding the murine D4 dopamine receptor. FEBS Lett., 361: 215-219. [PMID:7698326]

18. Gazi L, Bobirnac I, Danzeisen M, Schüpbach E, Bruinvels AT, Geisse S, Sommer B, Hoyer D, Tricklebank M, Schoeffter P. (1998) The agonist activities of the putative antipsychotic agents, L-745,870 and U-101958 in HEK293 cells expressing the human dopamine D4.4 receptor. Br. J. Pharmacol., 124 (5): 889-96. [PMID:9692773]

19. Gazi L, Bobirnac I, Danzeisen M, Schüpbach E, Langenegger D, Sommer B, Hoyer D, Tricklebank M, Schoeffter P. (1999) Receptor density as a factor governing the efficacy of the dopamine D4 receptor ligands, L-745,870 and U-101958 at human recombinant D4.4 receptors expressed in CHO cells. Br. J. Pharmacol., 128 (3): 613-20. [PMID:10516640]

20. Gazi L, Schoeffter P, Nunn C, Croskery K, Hoyer D, Feuerbach D. (2000) Cloning, expression, functional coupling and pharmacological characterization of the rat dopamine D4 receptor. Naunyn Schmiedebergs Arch. Pharmacol., 361 (5): 555-64. [PMID:10832611]

21. Glavin GB, Hall AM. (1994) Clozapine, a dopamine DA4 receptor antagonist, reduces gastric acid secretion and stress-induced gastric mucosal injury. Life Sci., 54 (16): PL261-4. [PMID:8152334]

22. Grady DL, Thanos PK, Corrada MM, Barnett Jr JC, Ciobanu V, Shustarovich D, Napoli A, Moyzis AG, Grandy D, Rubinstein M et al.. (2013) DRD4 genotype predicts longevity in mouse and human. J. Neurosci., 33 (1): 286-91. [PMID:23283341]

23. Graziane NM, Yuen EY, Yan Z. (2009) Dopamine D4 Receptors Regulate GABAA Receptor Trafficking via an Actin/Cofilin/Myosin-dependent Mechanism. J. Biol. Chem., 284 (13): 8329-36. [PMID:19179335]

24. Hidaka K, Matsumoto M, Tada S, Tasaki Y, Yamaguchi T. (1995) Differential effects of [3H]nemonapride and [3H]spiperone binding on human dopamine D4 receptors. Neurosci. Lett., 186 (2-3): 145-8. [PMID:7777184]

25. Kim JS, Bailey MJ, Weller JL, Sugden D, Rath MF, Møller M, Klein DC. (2010) Thyroid hormone and adrenergic signaling interact to control pineal expression of the dopamine receptor D4 gene (Drd4). Mol. Cell. Endocrinol., 314 (1): 128-35. [PMID:19482058]

26. Kortagere S, Gmeiner P, Weinstein H, Schetz JA. (2004) Certain 1,4-disubstituted aromatic piperidines and piperazines with extreme selectivity for the dopamine D4 receptor interact with a common receptor microdomain. Mol. Pharmacol., 66 (6): 1491-9. [PMID:15448188]

27. Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguère PM, Sciaky N, Roth BL. (2015) PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Nat. Struct. Mol. Biol., 22 (5): 362-9. [PMID:25895059]

28. Kulagowski JJ, Broughton HB, Curtis NR, Mawer IM, Ridgill MP, Baker R, Emms F, Freedman SB, Marwood R, Patel S et al.. (1996) 3-((4-(4-Chlorophenyl)piperazin-1-yl)-methyl)-1H-pyrrolo-2,3-b-pyridine: an antagonist with high affinity and selectivity for the human dopamine D4 receptor. J. Med. Chem., 39 (10): 1941-2. [PMID:8642550]

29. Lahti RA, Evans DL, Stratman NC, Figur LM. (1993) Dopamine D4 versus D2 receptor selectivity of dopamine receptor antagonists: possible therapeutic implications. Eur. J. Pharmacol., 236 (3): 483-6. [PMID:8102973]

30. Lanau F, Zenner MT, Civelli O, Hartman DS. (1997) Epinephrine and norepinephrine act as potent agonists at the recombinant human dopamine D4 receptor. J. Neurochem., 68 (2): 804-12. [PMID:9003072]

31. Livak KJ, Rogers J, Lichter JB. (1995) Variability of dopamine D4 receptor (DRD4) gene sequence within and among nonhuman primate species. Proc. Natl. Acad. Sci. U.S.A., 92 (2): 427-31. [PMID:7831304]

32. McAllister G, Knowles MR, Ward-Booth SM, Sinclair HA, Patel S, Marwood R, Emms F, Patel S, Smith A, Seabrook GR et al.. (1995) Functional coupling of human D2, D3, and D4 dopamine receptors in HEK293 cells. J. Recept. Signal Transduct. Res., 15 (1-4): 267-81. [PMID:8903944]

33. Meade JA, Free RB, Miller NR, Chun LS, Doyle TB, Moritz AE, Conroy JL, Watts VJ, Sibley DR. (2015) (-)-Stepholidine is a potent pan-dopamine receptor antagonist of both G protein- and β-arrestin-mediated signaling. Psychopharmacology (Berl.), 232 (5): 917-30. [PMID:25231919]

34. Meador-Woodruff JH, Damask SP, Wang J, Haroutunian V, Davis KL, Watson SJ. (1996) Dopamine receptor mRNA expression in human striatum and neocortex. Neuropsychopharmacology, 15 (1): 17-29. [PMID:8797188]

35. Mei YA, Griffon N, Buquet C, Martres MP, Vaudry H, Schwartz JC, Sokoloff P, Cazin L. (1995) Activation of dopamine D4 receptor inhibits an L-type calcium current in cerebellar granule cells. Neuroscience, 68 (1): 107-16. [PMID:7477916]

36. Millan MJ, Maiofiss L, Cussac D, Audinot V, Boutin JA, Newman-Tancredi A. (2002) Differential actions of antiparkinson agents at multiple classes of monoaminergic receptor. I. A multivariate analysis of the binding profiles of 14 drugs at 21 native and cloned human receptor subtypes. J. Pharmacol. Exp. Ther., 303 (2): 791-804. [PMID:12388666]

37. Moreland RB, Patel M, Hsieh GC, Wetter JM, Marsh K, Brioni JD. (2005) A-412997 is a selective dopamine D4 receptor agonist in rats. Pharmacol. Biochem. Behav., 82 (1): 140-7. [PMID:16153699]

38. Mulcrone J, Kerwin RW. (1997) The regional pattern of D4 gene expression in human brain. Neurosci. Lett., 234 (2-3): 147-50. [PMID:9364519]

39. Nakane M, Cowart MD, Hsieh GC, Miller L, Uchic ME, Chang R, Terranova MA, Donnelly-Roberts DL, Namovic MT, Miller TR et al.. (2005) 2-[4-(3,4-Dimethylphenyl)piperazin-1-ylmethyl]-1H benzoimidazole (A-381393), a selective dopamine D4 receptor antagonist. Neuropharmacology, 49 (1): 112-21. [PMID:15992586]

40. Nir I, Harrison JM, Haque R, Low MJ, Grandy DK, Rubinstein M, Iuvone PM. (2002) Dysfunctional light-evoked regulation of cAMP in photoreceptors and abnormal retinal adaptation in mice lacking dopamine D4 receptors. J. Neurosci., 22 (6): 2063-73. [PMID:11896146]

41. O'Malley KL, Harmon S, Tang L, Todd RD. (1992) The rat dopamine D4 receptor: sequence, gene structure, and demonstration of expression in the cardiovascular system. New Biol., 4 (2): 137-46. [PMID:1554689]

42. Patel MV, Kolasa T, Mortell K, Matulenko MA, Hakeem AA, Rohde JJ, Nelson SL, Cowart MD, Nakane M, Miller LN et al.. (2006) Discovery of 3-methyl-N-(1-oxy-3',4',5',6'-tetrahydro-2'H-[2,4'-bipyridine]-1'-ylmethyl)benzamide (ABT-670), an orally bioavailable dopamine D4 agonist for the treatment of erectile dysfunction. J. Med. Chem., 49 (25): 7450-65. [PMID:17149874]

43. Patel S, Freedman S, Chapman KL, Emms F, Fletcher AE, Knowles M, Marwood R, Mcallister G, Myers J, Curtis N et al.. (1997) Biological profile of L-745,870, a selective antagonist with high affinity for the dopamine D4 receptor. J. Pharmacol. Exp. Ther., 283 (2): 636-47. [PMID:9353380]

44. Patel S, Patel S, Marwood R, Emms F, Marston D, Leeson PD, Curtis NR, Kulagowski JJ, Freedman SB. (1996) Identification and pharmacological characterization of [125I]L-750,667, a novel radioligand for the dopamine D4 receptor. Mol. Pharmacol., 50 (6): 1658-64. [PMID:8967990]

45. Pilla M, Perachon S, Sautel F, Garrido F, Mann A, Wermuth CG, Schwartz JC, Everitt BJ, Sokoloff P. (1999) Selective inhibition of cocaine-seeking behaviour by a partial dopamine D3 receptor agonist. Nature, 400 (6742): 371-5. [PMID:10432116]

46. Pillai G, Brown NA, McAllister G, Milligan G, Seabrook GR. (1998) Human D2 and D4 dopamine receptors couple through betagamma G-protein subunits to inwardly rectifying K+ channels (GIRK1) in a Xenopus oocyte expression system: selective antagonism by L-741,626 and L-745,870 respectively. Neuropharmacology, 37: 983-987. [PMID:9833627]

47. Primus RJ, Thurkauf A, Xu J, Yevich E, McInerney S, Shaw K, Tallman JF, Gallagher DW. (1997) II. Localization and characterization of dopamine D4 binding sites in rat and human brain by use of the novel, D4 receptor-selective ligand [3H]NGD 94-1. J. Pharmacol. Exp. Ther., 282 (2): 1020-7. [PMID:9262371]

48. Ricci A, Mignini F, Tomassoni D, Amenta F. (2006) Dopamine receptor subtypes in the human pulmonary arterial tree. Auton Autacoid Pharmacol, 26 (4): 361-9. [PMID:16968475]

49. Rivera A, Peñafiel A, Megías M, Agnati LF, López-Téllez JF, Gago B, Gutiérrez A, de la Calle A, Fuxe K. (2008) Cellular localization and distribution of dopamine D(4) receptors in the rat cerebral cortex and their relationship with the cortical dopaminergic and noradrenergic nerve terminal networks. Neuroscience, 155 (3): 997-1010. [PMID:18620029]

50. Rivera A, Trías S, Peñafiel A, Angel Narváez J, Díaz-Cabiale Z, Moratalla R, de la Calle A. (2003) Expression of D4 dopamine receptors in striatonigral and striatopallidal neurons in the rat striatum. Brain Res., 989 (1): 35-41. [PMID:14519509]

51. Rowley M, Broughton HB, Collins I, Baker R, Emms F, Marwood R, Patel S, Patel S, Ragan CI, Freedman SB et al.. (1996) 5-(4-Chlorophenyl)-4-methyl-3-(1-(2-phenylethyl)piperidin-4-yl)isoxazole: a potent, selective antagonist at human cloned dopamine D4 receptors. J. Med. Chem., 39 (10): 1943-5. [PMID:8642551]

52. Rubinstein M, Phillips TJ, Bunzow JR, Falzone TL, Dziewczapolski G, Zhang G, Fang Y, Larson JL, McDougall JA, Chester JA, Saez C, Pugsley TA, Gershanik O, Low MJ, Grandy DK. (1997) Mice lacking dopamine D4 receptors are supersensitive to ethanol, cocaine, and methamphetamine. Cell, 90: 991-1001. [PMID:9323127]

53. Schetz JA, Benjamin PS, Sibley DR. (2000) Nonconserved residues in the second transmembrane-spanning domain of the D(4) dopamine receptor are molecular determinants of D(4)-selective pharmacology. Mol. Pharmacol., 57 (1): 144-52. [PMID:10617689]

54. Schlachter SK, Poel TJ, Lawson CF, Dinh DM, Lajiness ME, Romero AG, Rees SA, Duncan JN, Smith MW. (1997) Substituted 4-aminopiperidines having high in vitro affinity and selectivity for the cloned human dopamine D4 receptor. Eur. J. Pharmacol., 322 (2-3): 283-6. [PMID:9098699]

55. Schoots O, Van Tol HH. (2003) The human dopamine D4 receptor repeat sequences modulate expression. Pharmacogenomics J., 3 (6): 343-8. [PMID:14581929]

56. Schotte A, Janssen PF, Gommeren W, Luyten WH, Van Gompel P, Lesage AS, De Loore K, Leysen JE. (1996) Risperidone compared with new and reference antipsychotic drugs: in vitro and in vivo receptor binding. Psychopharmacology (Berl.), 124 (1-2): 57-73. [PMID:8935801]

57. Seeman P. (2001) Antipsychotic drugs, dopamine receptors, and schizophrenia. Clinical Neuroscience Research, 1 (1-2): 53-60. DOI: 10.1016/S1566-2772(00)00007-4

58. Seeman P, Corbett R, Van Tol HH. (1997) Atypical neuroleptics have low affinity for dopamine D2 receptors or are selective for D4 receptors. Neuropsychopharmacology, 16 (2): 93-110; discussion 111-35. [PMID:9015795]

59. Seeman P, Guan HC, Van Tol HH, Niznik HB. (1993) Low density of dopamine D4 receptors in Parkinson's, schizophrenia, and control brain striata. Synapse, 14: 247-253. [PMID:8248849]

60. Seeman P, Tallerico T. (1998) Antipsychotic drugs which elicit little or no parkinsonism bind more loosely than dopamine to brain D2 receptors, yet occupy high levels of these receptors. Mol. Psychiatry, 3 (2): 123-34. [PMID:9577836]

61. Shahid M, Walker GB, Zorn SH, Wong EH. (2009) Asenapine: a novel psychopharmacologic agent with a unique human receptor signature. J. Psychopharmacol. (Oxford), 23 (1): 65-73. [PMID:18308814]

62. Shih YH, Chung FZ, Pugsley TA. (1997) Cloning, expression and characterization of a human dopamine D4.2 receptor (CHO K1 cells) and various D4.2/D2L chimeras (COS-7 cells). Prog. Neuropsychopharmacol. Biol. Psychiatry, 21 (1): 153-67. [PMID:9075264]

63. Shin Y, Kumar U, Patel Y, Patel SC, Sidhu A. (2003) Differential expression of D2-like dopamine receptors in the kidney of the spontaneously hypertensive rat. J. Hypertens., 21 (1): 199-207. [PMID:12544452]

64. Skaaning Jensen B, Levavi-Sivan B, Fishburn CS, Fuchs S. (1997) Functional expression of the murine D2, D3, and D4 dopamine receptors in Xenopus laevis oocytes. FEBS Lett, 420: 191-195. [PMID:9459308]

65. Sun D, Wilborn TW, Schafer JA. (1998) Dopamine D4 receptor isoform mRNA and protein are expressed in the rat cortical collecting duct. Am. J. Physiol., 275 (5): F742-51. [PMID:9815131]

66. Sunohara GA, Roberts W, Malone M, Schachar RJ, Tannock R, Basile VS, Wigal T, Wigal SB, Schuck S, Moriarty J, Swanson JM, Kennedy JL, Barr CL. (2000) Linkage of the dopamine D4 receptor gene and attention-deficit/hyperactivity disorder. J Am Acad Child Adolesc Psychiatry, 39: 1537-1542. [PMID:11128331]

67. Suzuki T, Kobayashi K, Nagatsu T. (1995) Genomic structure and tissue distribution of the mouse dopamine D4 receptor. Neurosci Lett, 199: 69-72. [PMID:8584230]

68. Svingos AL, Periasamy S, Pickel VM. (2000) Presynaptic dopamine D(4) receptor localization in the rat nucleus accumbens shell. Synapse, 36 (3): 222-32. [PMID:10819901]

69. Tallman JF, Primus RJ, Brodbeck R, Cornfield L, Meade R, Woodruff K, Ross P, Thurkauf A, Gallager DW. (1997) I. NGD 94-1: identification of a novel, high-affinity antagonist at the human dopamine D4 receptor. J. Pharmacol. Exp. Ther., 282 (2): 1011-9. [PMID:9262370]

70. Tang L, Todd RD, Heller A, O'Malley KL. (1994) Pharmacological and functional characterization of D2, D3 and D4 dopamine receptors in fibroblast and dopaminergic cell lines. J. Pharmacol. Exp. Ther., 268 (1): 495-502. [PMID:8301592]

71. Thomas TC, Grandy DK, Gerhardt GA, Glaser PE. (2009) Decreased dopamine D4 receptor expression increases extracellular glutamate and alters its regulation in mouse striatum. Neuropsychopharmacology, 34 (2): 436-45. [PMID:18536704]

72. Thomas TC, Kruzich PJ, Joyce BM, Gash CR, Suchland K, Surgener SP, Rutherford EC, Grandy DK, Gerhardt GA, Glaser PE. (2007) Dopamine D4 receptor knockout mice exhibit neurochemical changes consistent with decreased dopamine release. J. Neurosci. Methods, 166 (2): 306-14. [PMID:17449106]

73. Tice MA, Hashemi T, Taylor LA, Duffy RA, McQuade RD. (1994) Characterization of the binding of SCH 39166 to the five cloned dopamine receptor subtypes. Pharmacol. Biochem. Behav., 49 (3): 567-71. [PMID:7862709]

74. Van Tol HH, Bunzow JR, Guan HC, Sunahara RK, Seeman P, Niznik HB, Civelli O. (1991) Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine. Nature, 350 (6319): 610-4. [PMID:1840645]

75. Van Tol HH, Wu CM, Guan HC, Ohara K, Bunzow JR, Civelli O, Kennedy J, Seeman P, Niznik HB, Jovanovic V. (1992) Multiple dopamine D4 receptor variants in the human population. Nature, 358 (6382): 149-52. [PMID:1319557]

76. van Vliet LA, Tepper PG, Dijkstra D, Damsma G, Wikström H, Pugsley TA, Akunne HC, Heffner TG, Glase SA, Wise LD. (1996) Affinity for dopamine D2, D3, and D4 receptors of 2-aminotetralins. Relevance of D2 agonist binding for determination of receptor subtype selectivity. J. Med. Chem., 39 (21): 4233-7. [PMID:8863800]

77. Wang S, Wacker D, Levit A, Che T, Betz RM, McCorvy JD, Venkatakrishnan AJ, Huang XP, Dror RO, Shoichet BK et al.. (2017) D4 dopamine receptor high-resolution structures enable the discovery of selective agonists. Science, 358 (6361): 381-386. [PMID:29051383]

78. Wedemeyer C, Goutman JD, Avale ME, Franchini LF, Rubinstein M, Calvo DJ. (2007) Functional activation by central monoamines of human dopamine D(4) receptor polymorphic variants coupled to GIRK channels in Xenopus oocytes. Eur. J. Pharmacol., 562 (3): 165-73. [PMID:17350612]

79. Yuen EY, Zhong P, Yan Z. (2010) Homeostatic regulation of glutamatergic transmission by dopamine D4 receptors. Proc. Natl. Acad. Sci. U.S.A., 107 (51): 22308-13. [PMID:21135234]

80. Zarei S, Frieden M, Rubi B, Villemin P, Gauthier BR, Maechler P, Vischer UM. (2006) Dopamine modulates von Willebrand factor secretion in endothelial cells via D2-D4 receptors. J. Thromb. Haemost., 4 (7): 1588-95. [PMID:16839358]

81. Zhong P, Yan Z. (2016) Distinct Physiological Effects of Dopamine D4 Receptors on Prefrontal Cortical Pyramidal Neurons and Fast-Spiking Interneurons. Cereb. Cortex, 26 (1): 180-91. [PMID:25146372]

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