atropine [Ligand Id: 320] activity data from GtoPdb and ChEMBL
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| ChEMBL ligand: CHEMBL517712 (Atroject, Atropen, Atrophate, Atropine, Atropine (autoinjector), Atropinum, Dl-hyoscyamine, Isopto Atropine) |
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| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3198] [GtoPdb: 2465] [UniProtKB: P21836] | ||||||||
| ChEMBL | Compound was evaluated for the binding affinity by displacing [3H]methylscopolamine [3H]NMS from mouse cerebral cortex tissue. | B | 9.15 | pKi | 0.7 | nM | Ki | J Med Chem (1992) 35: 1102-1108 [PMID:1552502] |
| ChEMBL | Compound was evaluated for the binding affinity by displacing [3H]oxotremorine from mouse cerebral cortex tissue. | B | 9.46 | pKi | 0.35 | nM | Ki | J Med Chem (1992) 35: 1102-1108 [PMID:1552502] |
| ADGRF1/Adhesion G-protein coupled receptor F1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523872] [GtoPdb: 190] [UniProtKB: Q5T601] | ||||||||
| ChEMBL | GPCR PRESTO-Tango dose-response in antagonist mode with target: ADGRF1 | F | 5 | pIC50 | >10000 | nM | IC50 | EUbOPEN Chemogenomics Library - GPCR Dose-Respose |
| α1D-adrenoceptor/Alpha-1d adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL223] [GtoPdb: 24] [UniProtKB: P25100] | ||||||||
| ChEMBL | DRUGMATRIX: Alpha-1D adrenergic receptor radioligand binding (ligand: prazosin) | B | 6.18 | pKi | 666 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | DRUGMATRIX: Alpha-1D adrenergic receptor radioligand binding (ligand: prazosin) | B | 5.87 | pIC50 | 1354 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229] | ||||||||
| ChEMBL | Binding affinity towards muscarinic m1 receptor | B | 8.7 | pKi | 2 | nM | Ki | J Med Chem (1994) 37: 2285-2291 [PMID:8057277] |
| ChEMBL | Competitive inhibition of EGFP-fused human M1 receptor N-terminal truncated at 17 residues expressed in HEK293 cells after 4 hrs by FRET assay in presence of para-LRB-AC42 | B | 8.92 | pKi | 1.2 | nM | Ki | J Med Chem (2012) 55: 2125-2143 [PMID:22329602] |
| ChEMBL | Displacement of [3H] N-methylscopolamine from human muscarinic M1 receptor expressed in CHOK1 cells after 30 mins by scintillation counting analysis | B | 9.04 | pKi | 0.92 | nM | Ki | Bioorg Med Chem (2013) 21: 2651-2662 [PMID:23523385] |
| ChEMBL | Displacement of [3H]NMS from EGFP-fused human M1 receptor N-terminal truncated at 17 residues expressed in HEK293 cells after 22 hrs by liquid scintillation counting | B | 9.24 | pKi | 0.58 | nM | Ki | J Med Chem (2012) 55: 2125-2143 [PMID:22329602] |
| ChEMBL | Displacement of [3H]NMS from human M1 AChR expressed in CHO cell membranes after 1 to 2 hrs by liquid scintillation spectrometry method | B | 9.36 | pKi | 0.44 | nM | Ki | Bioorg Med Chem Lett (2019) 29: 471-476 [PMID:30554957] |
| ChEMBL | DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.44 | pKi | 0.37 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | Inhibition of [3H]QNB binding to CHO cells bearing transfected muscarinic acetylcholine receptor M3 | B | 9.48 | pKi | 0.33 | nM | Ki | J Med Chem (1991) 34: 1436-1440 [PMID:2016720] |
| ChEMBL | Displacement of [3H]pirenzepine from muscarinic M1 receptor | B | 9.52 | pKi | 0.3 | nM | Ki | J Nat Prod (1993) 56: 441-455 [PMID:8496700] |
| GtoPdb | - | - | 9.6 | pKi | - | - | - |
EMBO J (1987) 6: 3923-9 [PMID:3443095]; Mol Pharmacol (1998) 53: 1120-30 [PMID:9614217]; AAPS PharmSci (2001) 3: E30 [PMID:12049493]; Mol Pharmacol (2006) 69: 1641-51 [PMID:16439611]; J Pharmacol Exp Ther (2001) 297: 790-7 [PMID:11303071]; J Recept Signal Transduct Res (1997) 17: 177-84 [PMID:9029489] |
| ChEMBL | Binding affinity (Ki) against binding of [3H]NMS using membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M1 | B | 9.6 | pKi | 0.25 | nM | Ki | J Med Chem (2002) 45: 984-987 [PMID:11831911] |
| ChEMBL | DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 8.82 | pIC50 | 1.52 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| Muscarinic acetylcholine receptor M1 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2672] [UniProtKB: Q8WMX0] | ||||||||
| ChEMBL | Inhibition of [3H]NMS binding to cerebral cortex membranes which contain predominantly the Muscarinic acetylcholine receptor M1 subtypes | B | 8.62 | pKi | 2.4 | nM | Ki | J Med Chem (1989) 32: 1522-1528 [PMID:2738887] |
| ChEMBL | Binding affinity against Muscarinic acetylcholine receptor M1 by displacement of [3H]pirenzepine in bovine striatum | B | 9.7 | pKi | 0.2 | nM | Ki | J Med Chem (1988) 31: 1463-1466 [PMID:3385735] |
| M1 receptor/Muscarinic acetylcholine receptor M1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL276] [GtoPdb: 13] [UniProtKB: P08482] | ||||||||
| ChEMBL | In vitro receptor binding against Muscarinic acetylcholine receptor M1 in rat cerebral cortex was determined using [3H]pirenzepine | B | 8.89 | pKd | 1.3 | nM | Kd | J Med Chem (1990) 33: 2108-2113 [PMID:2374141] |
| ChEMBL | Displacement of [3H]NMS from rat muscarinic M1 receptor expressed in CHO cells after 3 hrs | B | 8.85 | pKi | 1.4 | nM | Ki | Bioorg Med Chem Lett (2013) 23: 223-227 [PMID:23200253] |
| ChEMBL | Displacement of [3H]NMS from rat muscarinic M1 receptor expressed in CHO cells after 3 hrs | B | 8.86 | pKi | 1.38 | nM | Ki | Bioorg Med Chem Lett (2013) 23: 223-227 [PMID:23200253] |
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M1 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 9.1 | pKi | 0.79 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| ChEMBL | Evaluated for the phosphatidyl inositol turnover at Muscarinic acetylcholine receptor M1 in rat cortex | F | 9.3 | pKi | 0.5 | nM | Ki | J Med Chem (1988) 31: 1463-1466 [PMID:3385735] |
| ChEMBL | Ability to displace [3H]pirenzepine (PZ) from M1 receptor in rat cortex homogenate | B | 9.59 | pKi | 0.26 | nM | Ki | J Med Chem (1993) 36: 848-854 [PMID:8464039] |
| ChEMBL | Displacement of [3H]pirenzepine from muscarinic acetylcholine receptor M1 in rat cortex homogenates | B | 9.59 | pKi | 0.26 | nM | Ki | J Med Chem (1991) 34: 2984-2989 [PMID:1920350] |
| GtoPdb | - | - | 9.7 | pKi | - | - | - |
J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229]; Life Sci (1992) 51: 955-71 [PMID:1325587]; Mol Pharmacol (1989) 35: 469-76 [PMID:2704370] |
| M2 receptor/Muscarinic acetylcholine receptor M2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172] | ||||||||
| ChEMBL | Antimuscarinic activity on the acetylcholine-induced inhibition of contraction of guinea pig ileum which has M2 muscarinic receptor subtype. | F | 8.7 | pKd | 2 | nM | Kd | J Med Chem (1989) 32: 1522-1528 [PMID:2738887] |
| ChEMBL | Antagonist potency against muscarinic receptors was assed by antagonism of carbachol induced inhibition of electrically stimulated guinea pig atria | F | 8.87 | pKd | 1.35 | nM | Kd | J Med Chem (1989) 32: 79-84 [PMID:2909747] |
| ChEMBL | Displacement of 4-(2-((1E,3E)-5-((E)-3,3-Dimethyl-1-(6-oxo-6-((2-(4-(4-(1-(2-oxo-2-(11-oxo-10,11-dihydro-5H-dibenzo[b,e][1,4]diazepin-5-yl)ethyl)piperidin-4-yl)butyl)piperazin-1-yl)ethyl)amino)hexyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-1-yl)butane-1-sulfonate Tris(hydrotrifluoroacetate fluorescent tracer from human muscarinic M2 receptor expressed in CHO-K9 cells measured after 60 mins by Hoechst H33342 dye based confocal plate reader assay | B | 7.73 | pKi | 18.62 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of [3H]UR-AP060 from human muscarinic acetylcholine receptor M2 expressed in CHOK9 cell homogenate after 3 hrs by liquid scintillation counting assay | B | 7.77 | pKi | 16.98 | nM | Ki | J Med Chem (2017) 60: 3314-3334 [PMID:28388054] |
| ChEMBL | Displacement of [3H]NMS from human muscarinic acetylcholine receptor M2 expressed in CHOK1 cells | B | 7.8 | pKi | 15.85 | nM | Ki | J Med Chem (2017) 60: 3314-3334 [PMID:28388054] |
| ChEMBL | Displacement of 4-(2-((1E,3E)-5-((E)-1-(6-((3,5-Bis((2-(3-(1-(4-(1-(2-oxo-2-(11-oxo-10,11-dihydro-5H-dibenzo[b,e][1,4]diazepin-5-yl)ethyl)piperidin-4-yl)butyl)-1H-imidazol-4-yl)propanamido)ethyl)carbamoyl)benzyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-1-yl)butane-1-sulfonate Tris(hydrotrifluoroacetate) fluorescent tracer from human muscarinic M2 receptor expressed in CHO-K9 cells measured after 60 mins by Hoechst H33342 dye based confocal plate reader assay | B | 8.15 | pKi | 7.08 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of 4-(2-((1E,3E)-5-((E)-3,3-Dimethyl-1-(6-oxo-6-((2-(4-(4-(1-(2-oxo-2-(11-oxo-10,11-dihydro-5H-dibenzo[b,e][1,4]diazepin-5-yl)ethyl)piperidin-4-yl)butyl)piperazin-1-yl)ethyl)amino)hexyl)-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-1-yl)butane-1-sulfonate Bis(hydrotrifluoroacetate) fluorescent tracer from human muscarinic M2 receptor expressed in CHO-K9 cells measured after 60 mins by Hoechst H33342 dye based confocal plate reader assay | B | 8.31 | pKi | 4.9 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of 4-(2-((1E,3E)-5-((E)-3,3-Dimethyl-1-(6-oxo-6-((2-(3-(1-(4-(1-(2-oxo-2-(11-oxo-10,11-dihydro-5H-dibenzo[b,e][1,4]diazepin-5-yl)ethyl)piperidin-4-yl)butyl)-1H-imidazol-4-yl)propanamido)ethyl)-amino)hexyl)-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-1-yl)butane-1-sulfonateHydrotrifluoroacetate fluorescent tracer from human muscarinic M2 receptor expressed in CHO-K9 cells measured after 60 mins by Hoechst H33342 dye based confocal plate reader assay | B | 8.5 | pKi | 3.16 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of 4-(2-((1E,3E)-5-((E)-3,3-Dimethyl-1-(6-oxo-6-((2-(4-(4-(1-(2-oxo-2-(11-oxo-10,11-dihydro-5H-dibenzo[b,e][1,4]diazepin-5-yl)ethyl)piperidin-4-yl)butyl)piperazin-1-yl)ethyl)amino)hexyl)-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-1-yl)butane-1-sulfonate Bis(hydrotrifluoroacetate) fluorescent tracer from human muscarinic M2 receptor expressed in CHO-K9 cells by FACSCalibur flow cytometry | B | 8.6 | pKi | 2.51 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of [3H]-AFDX-384 from human muscarinic M2 receptor expressed in CHO-K1 cells | B | 8.72 | pKi | 1.9 | nM | Ki | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Binding affinity (Ki) against binding of [3H]NMS to membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M2 | B | 8.82 | pKi | 1.5 | nM | Ki | J Med Chem (2002) 45: 984-987 [PMID:11831911] |
| ChEMBL | Displacement of [3H] N-methylscopolamine from human muscarinic M2 receptor expressed in CHOK1 cells after 30 mins by scintillation counting analysis | B | 8.84 | pKi | 1.44 | nM | Ki | Bioorg Med Chem (2013) 21: 2651-2662 [PMID:23523385] |
| ChEMBL | DRUGMATRIX: Muscarinic M2 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 8.88 | pKi | 1.33 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | Binding affinity towards muscarinic m2 receptor | B | 9 | pKi | 1 | nM | Ki | J Med Chem (1994) 37: 2285-2291 [PMID:8057277] |
| ChEMBL | Displacement of [3H]-NMS from human muscarinic M2 receptor expressed in CHO-K9 cells measured after 60 mins by Hoechst H33342 dye based confocal plate reader assay | B | 9.04 | pKi | 0.91 | nM | Ki | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | Displacement of [3H]NMS from human M2 AChR expressed in CHO cell membranes after 1 to 2 hrs by liquid scintillation spectrometry method | B | 9.05 | pKi | 0.9 | nM | Ki | Bioorg Med Chem Lett (2019) 29: 471-476 [PMID:30554957] |
| ChEMBL | Displacement of [3H]UNSW-MK259 from human muscarinic acetylcholine receptor M2 expressed in CHOK9 cells after 3 hrs by liquid scintillation counting assay | B | 9.09 | pKi | 0.81 | nM | Ki | J Med Chem (2017) 60: 3314-3334 [PMID:28388054] |
| GtoPdb | - | - | 9.2 | pKi | - | - | - |
EMBO J (1987) 6: 3923-9 [PMID:3443095]; AAPS PharmSci (2001) 3: E30 [PMID:12049493]; J Pharmacol Exp Ther (2001) 297: 790-7 [PMID:11303071]; Eur J Pharmacol (2016) 782: 70-6 [PMID:27085897] |
| ChEMBL | muscarinic acetylcholine receptor M1 | B | 9.46 | pKi | 0.34 | nM | Ki | J Med Chem (1991) 34: 1436-1440 [PMID:2016720] |
| ChEMBL | GPCR PRESTO-Tango dose-response in antagonist mode with target: CHRM2 | F | 6.44 | pIC50 | 359.11 | nM | IC50 | EUbOPEN Chemogenomics Library - GPCR Dose-Respose |
| ChEMBL | Antagonist activity at human muscarinic M2 receptor expressed in HEK293 cells co-expressing HA-Galphaq/i5 assessed as inhibition of carbachol-induced IP1 accumulation pre-incubated for 30 mins followed by carbachol addition and measured after 1 hr by HTRF assay | F | 8.09 | pIC50 | 8.13 | nM | IC50 | J Med Chem (2019) 62: 5358-5369 [PMID:31074983] |
| ChEMBL | Antagonist activity at human muscarinic M2 receptor expressed in HEK293 cells co-expressing HA-Galphaq/i5 assessed as inhibition of carbachol-induced IP1 accumulation at 37 degree C pre-incubated for 30 mins followed by carbachol addition and measured after 60 mins by HTRF assay | F | 8.09 | pIC50 | 8.13 | nM | IC50 | J Med Chem (2020) 63: 4133-4154 [PMID:32233403] |
| ChEMBL | DRUGMATRIX: Muscarinic M2 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 8.43 | pIC50 | 3.73 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| M2 receptor/Muscarinic acetylcholine receptor M2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3197] [GtoPdb: 14] [UniProtKB: Q9ERZ4] | ||||||||
| ChEMBL | Ability to displace [3H]methylscopolamine ([3H]NMS) from mouse cerebral cortex | B | 9.15 | pKi | 0.7 | nM | Ki | J Med Chem (1992) 35: 3141-3147 [PMID:1507203] |
| ChEMBL | Ability to displace [3H]oxotremorine ([3H]-OXO-M) from mouse cerebral cortex | B | 9.46 | pKi | 0.35 | nM | Ki | J Med Chem (1992) 35: 3141-3147 [PMID:1507203] |
| M2 receptor/Muscarinic acetylcholine receptor M2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL309] [GtoPdb: 14] [UniProtKB: P10980] | ||||||||
| ChEMBL | In vitro receptor binding against Muscarinic acetylcholine receptor M2 in rat heart was determined using [3H]pirenzepine | B | 8.57 | pKd | 2.7 | nM | Kd | J Med Chem (1990) 33: 2108-2113 [PMID:2374141] |
| ChEMBL | Evaluated for the inhibition of adenylate cyclase at M2 receptor in rat heart | B | 8.12 | pKi | 7.5 | nM | Ki | J Med Chem (1988) 31: 1463-1466 [PMID:3385735] |
| ChEMBL | Inhibition of carbachol-induced release of alpha-amylase from pancreatic acinar cells from that of rat ileum contained the M2 receptor subtypes | B | 8.8 | pKi | 1.6 | nM | Ki | J Med Chem (1989) 32: 1522-1528 [PMID:2738887] |
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M2 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 8.84 | pKi | 1.45 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| GtoPdb | - | - | 9.1 | pKi | - | - | - |
Life Sci (1992) 51: 955-71 [PMID:1325587]; J Pharmacol Exp Ther (1998) 284: 500-7 [PMID:9454790] |
| ChEMBL | Ability to displace [3H](-)-quinuclidinyl benzilate(QNB) from M2 receptor in rat heart homogenate | B | 9.12 | pKi | 0.76 | nM | Ki | J Med Chem (1993) 36: 848-854 [PMID:8464039] |
| ChEMBL | Displacement of [3H](-)-quinuclidinyl benzilate(QNB) from muscarinic (M2) receptor in rat heart homogenates | B | 9.12 | pKi | 0.76 | nM | Ki | J Med Chem (1991) 34: 2984-2989 [PMID:1920350] |
| ChEMBL | Binding affinity against Muscarinic acetylcholine receptor M2 by displacement of [3H]QNB in rat myocardium | B | 9.15 | pKi | 0.7 | nM | Ki | J Med Chem (1988) 31: 1463-1466 [PMID:3385735] |
| M3 receptor/Muscarinic acetylcholine receptor M3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309] | ||||||||
| ChEMBL | Displacement of [3H] N-methylscopolamine from human muscarinic M3 receptor expressed in CHOK1 cells after 30 mins by scintillation counting analysis | B | 8.66 | pKi | 2.18 | nM | Ki | Bioorg Med Chem (2013) 21: 2651-2662 [PMID:23523385] |
| ChEMBL | Displacement of [3H]-4-DAMP from human muscarinic M3 receptor expressed in BHK-21 cells | B | 9.05 | pKi | 0.9 | nM | Ki | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Displacement of [3H]NMS from human M3 AChR expressed in CHO cell membranes after 1 to 2 hrs by liquid scintillation spectrometry method | B | 9.28 | pKi | 0.53 | nM | Ki | Bioorg Med Chem Lett (2019) 29: 471-476 [PMID:30554957] |
| ChEMBL | Binding affinity (Ki) against binding of [3H]NMS using membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M3 | B | 9.3 | pKi | 0.5 | nM | Ki | J Med Chem (2002) 45: 984-987 [PMID:11831911] |
| ChEMBL | DRUGMATRIX: Muscarinic M3 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.36 | pKi | 0.44 | nM | Ki | DrugMatrix in vitro pharmacology data |
| GtoPdb | - | - | 9.8 | pKi | - | - | - |
EMBO J (1987) 6: 3923-9 [PMID:3443095]; AAPS PharmSci (2001) 3: E30 [PMID:12049493]; J Pharmacol Exp Ther (2001) 297: 790-7 [PMID:11303071]; Eur J Pharmacol (2016) 782: 70-6 [PMID:27085897] |
| ChEMBL | [3H]-NMS Binding Assay: The binding of [3H]-NMS to human muscarinic receptors was performed according to Waelbroek et al (1990) (1). Assays were carried out at 25° C. Membrane preparations from stably transfected chinese hamster ovary-K1 cells (CHO) expressing the genes for the human muscarinic receptors Hm3 were used. | B | 8.49 | pIC50 | 3.2 | nM | IC50 | US-9333195-B2. Quinuclidine derivatives and medicinal compositions containing the same (2016) |
| ChEMBL | DRUGMATRIX: Muscarinic M3 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 8.68 | pIC50 | 2.07 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| M3 receptor/Muscarinic acetylcholine receptor M3 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL320] [GtoPdb: 15] [UniProtKB: P08483] | ||||||||
| ChEMBL | In vitro receptor binding against Muscarinic acetylcholine receptor M3 in rat submandibular gland was determined using [3H]pirenzepine | B | 9.2 | pKd | 0.63 | nM | Kd | J Med Chem (1990) 33: 2108-2113 [PMID:2374141] |
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M3 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 9.1 | pKi | 0.8 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| GtoPdb | - | - | 9.3 | pKi | - | - | - |
J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229]; Life Sci (1992) 51: 955-71 [PMID:1325587] |
| ChEMBL | Ability to displace [3H]N-methylscopolamine (NMS) from M3 receptor in rat submaxillary gland homogenate | B | 9.92 | pKi | 0.12 | nM | Ki | J Med Chem (1993) 36: 848-854 [PMID:8464039] |
| Muscarinic acetylcholine receptor M3 in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5498] [UniProtKB: Q8VH26] | ||||||||
| ChEMBL | Antagonist potency against carbachol induced contractions of isolated guinea pig ileum Muscarinic acetylcholine receptor | F | 8.83 | pKd | 1.48 | nM | Kd | J Med Chem (1989) 32: 79-84 [PMID:2909747] |
| ChEMBL | Antagonist potency at muscarinic M3 receptor in guinea-pig ileum assessed as inhibition of carbachol-induced contractions after 15 mins | F | 8.99 | pKd | 1.02 | nM | Kd | Bioorg Med Chem (2018) 26: 2573-2585 [PMID:29681486] |
| ChEMBL | Antagonist activity at muscarinic M3 receptor in guinea-pig ileum assessed as inhibition of carbachol-induced contractions after 15 mins | B | 8.99 | pKd | 1.02 | nM | Kd | Bioorg Med Chem (2019) 27: 1254-1262 [PMID:30792106] |
| ChEMBL | Antagonism to the H1 receptor of guinea pig ileum was determined | F | 9.11 | pKd | 0.78 | nM | Kd | Bioorg Med Chem Lett (1995) 5: 667-672 |
| M4 receptor/Muscarinic acetylcholine receptor M4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173] | ||||||||
| ChEMBL | Displacement of [3H]-4-DAMP from human muscarinic M4 receptor expressed in BHK-21 cells | B | 8.52 | pKi | 3 | nM | Ki | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Displacement of [3H]-NMS from recombinant human M4 receptor expressed in CHO cell membranes | B | 8.8 | pKi | 1.6 | nM | Ki | Bioorg Med Chem Lett (2017) 27: 2479-2483 [PMID:28427812] |
| ChEMBL | Displacement of [3H] N-methylscopolamine from human muscarinic M4 receptor expressed in CHOK1 cells after 30 mins by scintillation counting analysis | B | 8.86 | pKi | 1.39 | nM | Ki | Bioorg Med Chem (2013) 21: 2651-2662 [PMID:23523385] |
| ChEMBL | Displacement of [3H]NMS from human recombinant muscarinic M4 receptor expressed in CHO cell membranes | B | 9.19 | pKi | 0.64 | nM | Ki | Bioorg Med Chem Lett (2017) 27: 3576-3581 [PMID:28633897] |
| ChEMBL | Binding affinity (Ki) against binding of [3H]NMS using membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M4 | B | 9.47 | pKi | 0.34 | nM | Ki | J Med Chem (2002) 45: 984-987 [PMID:11831911] |
| GtoPdb | - | - | 9.5 | pKi | - | - | - |
EMBO J (1987) 6: 3923-9 [PMID:3443095]; AAPS PharmSci (2001) 3: E30 [PMID:12049493]; J Pharmacol Exp Ther (2001) 297: 790-7 [PMID:11303071]; Eur J Pharmacol (2016) 782: 70-6 [PMID:27085897] |
| ChEMBL | DRUGMATRIX: Muscarinic M4 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.98 | pKi | 0.1 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | DRUGMATRIX: Muscarinic M4 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.13 | pIC50 | 0.74 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| M4 receptor/Muscarinic acetylcholine receptor M4 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL317] [GtoPdb: 16] [UniProtKB: P08485] | ||||||||
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M4 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 9.28 | pKi | 0.52 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M4 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 9.28 | pKi | 0.52 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| GtoPdb | - | - | 9.7 | pKi | - | - | - | Life Sci (1992) 51: 955-71 [PMID:1325587] |
| M5 receptor/Muscarinic acetylcholine receptor M5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912] | ||||||||
| ChEMBL | Displacement of [3H]NMS from human muscarinic acetylcholine receptor subtype 5 expressed in CHO cell membranes by scintillation counting method | B | 8.83 | pKi | 1.47 | nM | Ki | J Med Chem (2014) 57: 7804-7810 [PMID:25147929] |
| ChEMBL | Displacement of [3H]-4-DAMP from human muscarinic M5 receptor expressed in BHK-21 cells | B | 9 | pKi | 1 | nM | Ki | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | DRUGMATRIX: Muscarinic M5 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.18 | pKi | 0.66 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | Displacement of [3H]NMS from human M5 AChR expressed in CHO cell membranes after 1 to 2 hrs by liquid scintillation spectrometry method | B | 9.22 | pKi | 0.6 | nM | Ki | Bioorg Med Chem Lett (2019) 29: 471-476 [PMID:30554957] |
| ChEMBL | Binding affinity (Ki) against binding of [3H]NMS using membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M5 | B | 9.27 | pKi | 0.54 | nM | Ki | J Med Chem (2002) 45: 984-987 [PMID:11831911] |
| GtoPdb | - | - | 9.3 | pKi | - | - | - |
J Pharmacol Exp Ther (2001) 297: 790-7 [PMID:11303071]; Eur J Pharmacol (2016) 782: 70-6 [PMID:27085897]; Life Sci (1999) 64: 2351-8 [PMID:10374898] |
| ChEMBL | Displacement of [3H]QNB from muscarinic acetylcholine M5 receptor after 1.5 hrs by scintillation counting | B | 9.4 | pKi | 0.4 | nM | Ki | Bioorg Med Chem (2011) 19: 5756-5762 [PMID:21907583] |
| ChEMBL | Activation of human muscarinic M5 receptor expressed in CHO cells coexpressing Gq protein assessed as increase in acetylcholine potency at 30 uM by calcium mobilization-based ACh concentration-response curve assay relative to control | F | 9.68 | pKi | 0.21 | nM | Ki | J Med Chem (2009) 52: 3445-3448 [PMID:19438238] |
| ChEMBL | DRUGMATRIX: Muscarinic M5 radioligand binding (ligand: [3H] N-Methylscopolamine) | B | 9.04 | pIC50 | 0.92 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| M5 receptor/Muscarinic acetylcholine receptor M5 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL277] [GtoPdb: 17] [UniProtKB: P08911] | ||||||||
| ChEMBL | Displacement of [3H]NMS from rat recombinant muscarinic M5 receptor expressed in CHO cells after 2 hrs by microplate scintillation counting | B | 9.07 | pKi | 0.86 | nM | Ki | Nat Chem Biol (2008) 4: 42-50 [PMID:18059262] |
| GtoPdb | - | - | 9.4 | pKi | - | - | - | Life Sci (1992) 51: 955-71 [PMID:1325587] |
| Nicotinic acetylcholine receptor alpha 5 subunit in Musca domestica (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366408] [UniProtKB: A9XFY4] | ||||||||
| ChEMBL | Displacement of [3H]alpha-bungarotoxin from nAChR in Musca domestica (house fly) head membrane after 60 min by scintillation counting | B | 4.49 | pKi | 32500 | nM | Ki | J Pestic Sci (1992) 17: 231-236 |
| 5-HT1A receptor/Serotonin 1a (5-HT1a) receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL273] [GtoPdb: 1] [UniProtKB: P19327] | ||||||||
| ChEMBL | Binding affinity towards 5-hydroxytryptamine 1A receptor by the displacement of [125I]trans-8-OH-PIPAT in membrane homogenates of hippocampal tissue of rat brain | B | 4.7 | pKi | >20000 | nM | Ki | J Med Chem (1993) 36: 3161-3165 [PMID:8230102] |
| 5-HT2C receptor/Serotonin 2c (5-HT2c) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL225] [GtoPdb: 8] [UniProtKB: P28335] | ||||||||
| ChEMBL | DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2C radioligand binding (ligand: [3H] Mesulergine) | B | 6.28 | pKi | 530 | nM | Ki | DrugMatrix in vitro pharmacology data |
| ChEMBL | DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2C radioligand binding (ligand: [3H] Mesulergine) | B | 5.99 | pIC50 | 1012 | nM | IC50 | DrugMatrix in vitro pharmacology data |
| Organic cation transporter 1/Solute carrier family 22 member 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5685] [GtoPdb: 1019] [UniProtKB: O15245] | ||||||||
| ChEMBL | Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy | B | 4.91 | pIC50 | 12200 | nM | IC50 | J Med Chem (2008) 51: 5932-5942 [PMID:18788725] |
| α2A-adrenoceptor in Human [GtoPdb: 25] [UniProtKB: P08913] | ||||||||
| GtoPdb | - | - | 4.85 | pKi | 14000 | nM | Ki | Invest Ophthalmol Vis Sci (2018) 59: 2778-2791 [PMID:29860464] |
| Glycine Receptor (All subtypes) in Human [GtoPdb: 428] | ||||||||
| GtoPdb | - | - | 3.6 | pIC50 | - | - | - |
J Neurochem (1999) 73: 802-6 [PMID:10428078]; J Neurochem (2007) 100: 758-69 [PMID:17181559] |
ChEMBL data shown on this page come from version 34:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
