berberine [Ligand Id: 11353] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL12089 (Berberine chloride, Berberine hydrochloride, Berberinum, GNF-Pf-4545, NSC-163088, NSC-646666)
  • Acetylcholinesterase in Electrophorus electricus [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
  • acetylcholinesterase (Cartwright blood group)/Acetylcholinesterase in Human [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
There should be some charts here, you may need to enable JavaScript!
  • α2B-adrenoceptor/Alpha-2b adrenergic receptor in Human [ChEMBL: CHEMBL1942] [GtoPdb: 26] [UniProtKB: P18089]
There should be some charts here, you may need to enable JavaScript!
  • α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825]
There should be some charts here, you may need to enable JavaScript!
  • butyrylcholinesterase/Butyrylcholinesterase in Human [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
  • Butyrylcholinesterase in Equus caballus [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9]
There should be some charts here, you may need to enable JavaScript!
  • Cell division protein ftsZ in Escherichia coli K-12 [ChEMBL: CHEMBL3999] [UniProtKB: P0A9A6]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • M1 receptor/Muscarinic acetylcholine receptor M1 in Human [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • Plasmodium falciparum (isolate K1 / Thailand) in Plasmodium falciparum K1 [ChEMBL: CHEMBL612856]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • Retinoid X receptor-α/Retinoid X receptor alpha in Human [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793]
There should be some charts here, you may need to enable JavaScript!
  • NIMA related kinase 7/Serine/threonine-protein kinase NEK7 in Human [ChEMBL: CHEMBL4849] [GtoPdb: 2122] [UniProtKB: Q8TDX7]
There should be some charts here, you may need to enable JavaScript!
  • 5-HT2B receptor/Serotonin 2b (5-HT2b) receptor in Human [ChEMBL: CHEMBL1833] [GtoPdb: 7] [UniProtKB: P41595]
There should be some charts here, you may need to enable JavaScript!
  • Serotonin 4 (5-HT4) receptor in Guinea pig [ChEMBL: CHEMBL5017] [UniProtKB: O70528]
There should be some charts here, you may need to enable JavaScript!
  • sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Acetylcholinesterase in Electrophorus electricus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
ChEMBL Inhibition of electric eel AChE by enzyme kinetics assay B 6.54 pKi 289 nM Ki Bioorg. Med. Chem. (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL Inhibition of electric eel AChE by Ellman's method B 5.56 pIC50 2740 nM IC50 Bioorg. Med. Chem. (2012) 20: 6669-6679 [PMID:23062825]
ChEMBL Inhibition of electric eel AChE by Ellman's assay B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. (2010) 18: 4475-4484 [PMID:20471843]
ChEMBL Inhibition of electric eel AChE after 15 mins by Ellman's method using acetylcholine as substrate B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. Lett. (2010) 20: 6649-6652 [PMID:20880702]
ChEMBL Inhibition of electric eel AChE by modified Ellman method B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL Inhibition of electric eel AChE using acetylcholine chloride as substrate preincubated for 15 mins by Ellman's method B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. (2011) 19: 7228-7235 [PMID:22041172]
ChEMBL Inhibition of electric eel AChE using acetylthiocholine chloride as substrate preincubated for 15 mins prior to substrate addition by Ellman's method B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. (2013) 21: 5830-5840 [PMID:23932451]
ChEMBL Inhibition of electric eel Acetylcholinesterase B 6.43 pIC50 374 nM IC50 Bioorg. Med. Chem. (2011) 19: 2298-2305 [PMID:21397508]
acetylcholinesterase (Cartwright blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
ChEMBL Inhibition of recombinant human acetylcholinesterase using acetylthiocholine iodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured at 2 mins interval by Ellman's method B 4.76 pIC50 17400 nM IC50 Eur J Med Chem (2020) 203: 112593-112593 [PMID:32688201]
ChEMBL DRUGMATRIX: Acetylcholinesterase enzyme inhibition (substrate: acetylthiocholine) B 6.11 pIC50 774 nM IC50 DrugMatrix in vitro pharmacology data
ChEMBL Inhibition of human AChE by Ellman's method B 6.21 pIC50 610 nM IC50 Bioorg. Med. Chem. (2012) 20: 6669-6679 [PMID:23062825]
ChEMBL Inhibition of AChE assessed as hydrolysis of acetylcholine preincubated for 15 mins measured after 15 mins by colorimetric Ellman assay B 7.4 pIC50 40 nM IC50 Bioorg. Med. Chem. Lett. (2011) 21: 6603-6607 [PMID:21924611]
α2B-adrenoceptor/Alpha-2b adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1942] [GtoPdb: 26] [UniProtKB: P18089]
ChEMBL DRUGMATRIX: Alpha-2B adrenergic receptor radioligand binding (ligand: Rauwolscine) B 6.56 pKi 274 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Alpha-2B adrenergic receptor radioligand binding (ligand: Rauwolscine) B 6.22 pIC50 601 nM IC50 DrugMatrix in vitro pharmacology data
α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825]
ChEMBL DRUGMATRIX: Adrenergic Alpha-2C radioligand binding (ligand: [3H] MK-912) B 6.39 pKi 412 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Adrenergic Alpha-2C radioligand binding (ligand: [3H] MK-912) B 5.55 pIC50 2837 nM IC50 DrugMatrix in vitro pharmacology data
butyrylcholinesterase/Butyrylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
ChEMBL Inhibition of human butyrylcholinesterase in plasma using butyrylthiocholineiodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured at 2 mins interval by Ellman's method B 4.33 pIC50 47200 nM IC50 Eur J Med Chem (2020) 203: 112593-112593 [PMID:32688201]
ChEMBL Inhibition of BChE assessed as hydrolysis of butrylcholine preincubated for 15 mins measured after 15 mins by colorimetric Ellman assay B 4.72 pIC50 18970 nM IC50 Bioorg. Med. Chem. Lett. (2011) 21: 6603-6607 [PMID:21924611]
Butyrylcholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9]
ChEMBL Inhibition of equine BChE after 15 mins by Ellman's method using acetylcholine as substrate B 4.74 pIC50 18200 nM IC50 Bioorg. Med. Chem. Lett. (2010) 20: 6649-6652 [PMID:20880702]
ChEMBL Inhibition of equine serum Butyrylcholinesterase B 4.74 pIC50 18200 nM IC50 Bioorg. Med. Chem. (2011) 19: 2298-2305 [PMID:21397508]
ChEMBL Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate preincubated for 15 mins by Ellman's method B 4.74 pIC50 18200 nM IC50 Bioorg. Med. Chem. (2011) 19: 7228-7235 [PMID:22041172]
Cell division protein ftsZ in Escherichia coli K-12 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3999] [UniProtKB: P0A9A6]
ChEMBL Displacement of bis-ANS from Escherichia coli FtsZ B 7.64 pKd 23 nM Kd J Med Chem (2016) 59: 6975-6998 [PMID:26756351]
ChEMBL Inhibition of recombinant Escherichia coli K12 FtsZ GTPase activity by malachite green sodium molybdate assay B 4.8 pIC50 16000 nM IC50 Bioorg Med Chem (2016) 24: 6354-6369 [PMID:27189886]
ChEMBL Inhibition of recombinant Escherichia coli K12 FtsZ assembly by light-scattering assay B 5 pIC50 10000 nM IC50 Bioorg Med Chem (2016) 24: 6354-6369 [PMID:27189886]
Cholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5763] [UniProtKB: P81908]
ChEMBL Inhibition of horse serum BChE by enzyme kinetics assay B 5.04 pKi 9200 nM Ki Bioorg. Med. Chem. (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL Inhibition of equine serum BChE using butylthiocholine chloride as substrate preincubated for 15 mins prior to substrate addition by Ellman's method B 4.74 pIC50 18210 nM IC50 Bioorg. Med. Chem. (2013) 21: 5830-5840 [PMID:23932451]
ChEMBL Inhibition of horse serum BChE by Ellman's method B 4.74 pIC50 18200 nM IC50 Bioorg. Med. Chem. (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL Inhibition of equine serum BuChE by Ellman's method B 4.74 pIC50 18200 nM IC50 Bioorg. Med. Chem. (2010) 18: 4475-4484 [PMID:20471843]
CYP2D6/Cytochrome P450 2D6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL289] [GtoPdb: 1329] [UniProtKB: P10635]
ChEMBL DRUGMATRIX: CYP450, 2D6 enzyme inhibition (substrate: 3-Cyano-7-ethoxycoumarin) B 5.35 pIC50 4504.2 nM IC50 DrugMatrix in vitro pharmacology data
GtoPdb - - 5.4 pIC50 - - - Pharmaceutics (2020) 12: [PMID:32987920]
Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL Inhibition of human ERG by patch clamp assay B 5.51 pIC50 3100 nM IC50 Eur J Med Chem (2017) 140: 448-464 [PMID:28987606]
M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
ChEMBL DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine) B 6.13 pKi 742 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine) B 5.51 pIC50 3080 nM IC50 DrugMatrix in vitro pharmacology data
NAD(+) hydrolase SARM1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523350] [UniProtKB: Q6SZW1]
ChEMBL Inhibition of recombinant human SARM1 SAM1-2-TIR domains (412 to 724 residues) expressed in Expi293 cells lysates using ENAD as substrate preincubated for 20 mins followed by ENAD addition and measured at 15 sec interval for 1 hr B 4.11 pIC50 77000 nM IC50 Bioorg Med Chem (2020) 28: 115644-115644 [PMID:32828421]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL NOVARTIS: Inhibition of Plasmodium falciparum W2 (drug-resistant) proliferation in erythrocyte-based infection assay F 6.35 pEC50 442 nM EC50 Proc. Natl. Acad. Sci. U.S.A. (2008) 105: 9059-9064 [PMID:18579783]
ChEMBL NOVARTIS: Inhibition of Plasmodium falciparum 3D7 (drug-susceptible) proliferation in erythrocyte-based infection assay F 6.66 pEC50 218 nM EC50 Proc. Natl. Acad. Sci. U.S.A. (2008) 105: 9059-9064 [PMID:18579783]
Plasmodium falciparum (isolate K1 / Thailand) in Plasmodium falciparum K1 (target type: ORGANISM) [ChEMBL: CHEMBL612856]
ChEMBL Antiplasmodial activity against multi drug-resistant Plasmodium falciparum K1 in erythrocytes by [3H]hypoxanthine uptake F 6.01 pIC50 968 nM IC50 J. Nat. Prod. (2000) 63: 1638-1640 [PMID:11141105]
Plasmodium yoelii (target type: ORGANISM) [ChEMBL: CHEMBL612889]
ChEMBL NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration F 6.26 pIC50 548 nM IC50 Science (2011) 334: 1372-1377 [PMID:22096101]
Retinoid X receptor-α/Retinoid X receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793]
ChEMBL Binding affinity to His-tagged RXRalpha LBD (unknown origin) expressed in Escherichia coli strain BL21 (DE3) by fluorescence quenching analysis B 4.61 pKd 24800 nM Kd J Med Chem (2020) 63: 5841-5855 [PMID:32391701]
NIMA related kinase 7/Serine/threonine-protein kinase NEK7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4849] [GtoPdb: 2122] [UniProtKB: Q8TDX7]
ChEMBL Binding affinity to purified recombinant human NEK7 by SPR analysis B 4.81 pKd 15600 nM Kd J Med Chem (2021) 64: 768-781 [PMID:33440945]
ChEMBL Inhibition of purified recombinant human NEK7 incubated for 15 mins before ATP and substrate addition and further incubated for 40 mins by ADPGlo kinase assay B 5.38 pIC50 4200 nM IC50 J Med Chem (2021) 64: 768-781 [PMID:33440945]
5-HT2B receptor/Serotonin 2b (5-HT2b) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1833] [GtoPdb: 7] [UniProtKB: P41595]
ChEMBL DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2B radioligand binding (ligand: [3H] Lysergic acid diethylamide) B 5.66 pKi 2187 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2B radioligand binding (ligand: [3H] Lysergic acid diethylamide) B 5.46 pIC50 3437 nM IC50 DrugMatrix in vitro pharmacology data
Serotonin 4 (5-HT4) receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5017] [UniProtKB: O70528]
ChEMBL DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT4 radioligand binding (ligand: [3H] GR-113808) B 6.43 pKi 370 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT4 radioligand binding (ligand: [3H] GR-113808) B 5.65 pIC50 2222 nM IC50 DrugMatrix in vitro pharmacology data
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720]
ChEMBL DRUGMATRIX: Sigma1 radioligand binding (ligand: [3H] Haloperidol) B 6.27 pKi 542 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL DRUGMATRIX: Sigma1 radioligand binding (ligand: [3H] Haloperidol) B 5.89 pIC50 1289 nM IC50 DrugMatrix in vitro pharmacology data

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]