orlistat [Ligand Id: 5277] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL175247 (Orlistat, Ro-180647002, Tetrahydrolipstatin, Beacita, Xenical, Lipstatin, ALLI, Orlipastat, RO-180647-002)
  • Fatty acid amide hydrolase/Anandamide amidohydrolase in Human [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519]
  • Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612]
There should be some charts here, you may need to enable JavaScript!
  • CB1 receptor/Cannabinoid CB1 receptor in Human [ChEMBL: CHEMBL218] [GtoPdb: 56] [UniProtKB: P21554]
There should be some charts here, you may need to enable JavaScript!
  • CB2 receptor/Cannabinoid CB2 receptor in Human [ChEMBL: CHEMBL253] [GtoPdb: 57] [UniProtKB: P34972]
There should be some charts here, you may need to enable JavaScript!
  • fatty acid synthase/Fatty acid synthase in Human [ChEMBL: CHEMBL4158] [GtoPdb: 2608] [UniProtKB: P49327]
There should be some charts here, you may need to enable JavaScript!
  • PLA2-G7/LDL-associated phospholipase A2 in Human [ChEMBL: CHEMBL3514] [GtoPdb: 1432] [UniProtKB: Q13093]
There should be some charts here, you may need to enable JavaScript!
  • αβ-Hydrolase 12/Monoacylglycerol lipase ABHD12 in Human [ChEMBL: CHEMBL5516] [GtoPdb: 3070] [UniProtKB: Q8N2K0]
There should be some charts here, you may need to enable JavaScript!
  • αβ-Hydrolase 6/Monoacylglycerol lipase ABHD6 in Human [ChEMBL: CHEMBL2189127] [GtoPdb: 2919] [UniProtKB: Q9BV23]
There should be some charts here, you may need to enable JavaScript!
  • Neutral cholesterol ester hydrolase 1 in Human [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2]
There should be some charts here, you may need to enable JavaScript!
  • pancreatic lipase/Pancreatic lipase in Human [ChEMBL: CHEMBL1812] [GtoPdb: 2590] [UniProtKB: P16233]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • proteasome 20S subunit beta 1/Proteasome component C5 in Human [ChEMBL: CHEMBL4208] [GtoPdb: 2404] [UniProtKB: P20618]
There should be some charts here, you may need to enable JavaScript!
  • proteasome 20S subunit beta 2/Proteasome Macropain subunit in Human [ChEMBL: CHEMBL3492] [GtoPdb: 2405] [UniProtKB: P49721]
There should be some charts here, you may need to enable JavaScript!
  • proteasome 20S subunit beta 5/Proteasome Macropain subunit MB1 in Human [ChEMBL: CHEMBL4662] [GtoPdb: 2406] [UniProtKB: P28074]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • Diacylglycerol lipase α/Sn1-specific diacylglycerol lipase alpha in Human [ChEMBL: CHEMBL5545] [GtoPdb: 1396] [UniProtKB: Q9Y4D2]
  • Diacylglycerol lipase α/Sn1-specific diacylglycerol lipase alpha in Mouse [ChEMBL: CHEMBL5180] [GtoPdb: 1396] [UniProtKB: Q6WQJ1]
There should be some charts here, you may need to enable JavaScript!
  • Diacylglycerol lipase β/Sn1-specific diacylglycerol lipase beta in Human [ChEMBL: CHEMBL5521] [GtoPdb: 1397] [UniProtKB: Q8NCG7]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519]
ChEMBL Inhibition of recombinant FAAH transfected in HEK293T cells by SDS-PAGE using rhodamine-tagged FP probe B 4 pIC50 >100000 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612]
ChEMBL Inhibition of FAAH-mediated [14C]anandamide hydrolysis in rat brain membrane B 4.3 pIC50 >50000 nM IC50 J. Med. Chem. (2008) 51: 6970-6979 [PMID:18831576]
ChEMBL Inhibition of rat histidine tagged FAAH expressed in Escherichia coli by coumarin ester substrate fluorescence assay B 8 pIC50 10 nM IC50 Bioorg. Med. Chem. Lett. (2012) 22: 4585-4592 [PMID:22738638]
CB1 receptor/Cannabinoid CB1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL218] [GtoPdb: 56] [UniProtKB: P21554]
ChEMBL Displacement of [3H]CP-55940 from human recombinant CB1 receptor expressed in HEK293 cells by scintillation counting B 5.6 pKi 2500 nM Ki J. Med. Chem. (2008) 51: 6970-6979 [PMID:18831576]
ChEMBL Binding affinity for cannabinoid receptor 1 B 7 pIC50 <=100 nM IC50 J. Med. Chem. (2005) 48: 5059-5087 [PMID:16078824]
CB2 receptor/Cannabinoid CB2 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL253] [GtoPdb: 57] [UniProtKB: P34972]
ChEMBL Displacement of [3H]CP-55940 from human recombinant CB2 receptor expressed in HEK293 cells by scintillation counting B 5 pKi >10000 nM Ki J. Med. Chem. (2008) 51: 6970-6979 [PMID:18831576]
fatty acid synthase/Fatty acid synthase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4158] [GtoPdb: 2608] [UniProtKB: P49327]
ChEMBL Antagonist activity at recombinant FASN thioesterase domain by fluorogenic assay F 6.55 pKi 280 nM Ki J. Med. Chem. (2011) 54: 5615-5638 [PMID:21726077]
ChEMBL Inhibition of human FAS thioster domain preincubated for 30 mins followed by 4-methylumbelliferyl heptanoate substrate addition measured every 5 mins for 60 mins by fluorescence assay B 5.33 pIC50 4630 nM IC50 Bioorg Med Chem (2017) 25: 2901-2916 [PMID:28236510]
PLA2-G7/LDL-associated phospholipase A2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3514] [GtoPdb: 1432] [UniProtKB: Q13093]
ChEMBL Inhibition of recombinant PLA2g7 transfected in HEK293T cells by SDS-PAGE using rhodamine-tagged FP probe B 7.3 pIC50 50 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
αβ-Hydrolase 12/Monoacylglycerol lipase ABHD12 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5516] [GtoPdb: 3070] [UniProtKB: Q8N2K0]
ChEMBL Inhibition of human ABHD12 containing pCMV6-XL4-hABHD12 transfected into HEK293 cells B 6.72 pIC50 190.55 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1436-1442 [PMID:25752982]
ChEMBL Inhibition of human ABHD12 expressed in HEK293 cells pre-incubated for 10 mins before 2-AG substrate addition by HPLC method B 6.72 pIC50 190.55 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
ChEMBL Inhibition of human ABHD12 containing pCMV6-XL4-hABHD12 transfected into HEK293 cells B 6.72 pIC50 190 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1436-1442 [PMID:25752982]
ChEMBL Inhibition of human ABHD12 expressed in HEK293 cells pre-incubated for 10 mins before 2-AG substrate addition by HPLC method B 6.72 pIC50 190 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
ChEMBL Inhibition of recombinant ABHD12 transfected in HEK293T cells by SDS-PAGE using rhodamine-tagged FP probe B 7.1 pIC50 80 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
GtoPdb Inhibition of recombinant ABHD12 expressed in HEK293T cells, by SDS-PAGE using rhodamine-tagged FP probe. - 7.1 pIC50 80 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-41 [PMID:18657971]
αβ-Hydrolase 6/Monoacylglycerol lipase ABHD6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2189127] [GtoPdb: 2919] [UniProtKB: Q9BV23]
ChEMBL Inhibition of human ABHD6 containing pCMV6-AC-hABHD6 transfected into HEK293 cells B 7.32 pIC50 48 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1436-1442 [PMID:25752982]
ChEMBL Inhibition of human ABHD6 containing pCMV6-AC-hABHD6 transfected into HEK293 cells B 7.32 pIC50 47.86 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1436-1442 [PMID:25752982]
ChEMBL Inhibition of human ABHD6 expressed in HEK293 cells at 1 uM pre-incubated for 10 mins before 2-AG substrate addition followed by rapid 40 fold compound dilution measured after 90 mins by HPLC method B 7.43 pIC50 37.15 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
ChEMBL Inhibition of human ABHD6 expressed in HEK293 cells at 1 uM pre-incubated for 10 mins before 2-AG substrate addition followed by rapid 40 fold compound dilution measured after 60 mins by HPLC method B 7.7 pIC50 19.95 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
ChEMBL Inhibition of human ABHD6 expressed in HEK293 cells at 1 uM pre-incubated for 10 mins before 2-AG substrate addition followed by rapid 40 fold compound dilution measured after 30 mins by HPLC method B 7.85 pIC50 14.13 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
ChEMBL Inhibition of human ABHD6 expressed in HEK293 cells at 1 uM pre-incubated for 10 mins before 2-AG substrate addition followed by rapid 40 fold compound dilution measured after 10 mins by HPLC method B 7.88 pIC50 13.18 nM IC50 Bioorg. Med. Chem. (2015) 23: 6335-6345 [PMID:26344596]
Neutral cholesterol ester hydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2]
ChEMBL Inhibition of recombinant KIAA1363 transfected in HEK293T cells by SDS-PAGE using rhodamine-tagged FP probe B 4 pIC50 >100000 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
pancreatic lipase/Pancreatic lipase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1812] [GtoPdb: 2590] [UniProtKB: P16233]
ChEMBL Inhibition of human pancreatic lipase using emulsified olive oil as substrate pretreated with substrate for 5 mins followed by enzyme addition and measured after 30 mins B 6.72 pIC50 190 nM IC50 ACS Med Chem Lett (2018) 9: 662-666 [PMID:30034597]
GtoPdb - - 8.9 pIC50 1.3 nM IC50 Journal of Medicinal Plants Research (2010) 4: 2235-2242
Pancreatic triacylglycerol lipase in Pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1687677] [UniProtKB: P00591]
ChEMBL Competitive inhibition of porcine pancreatic lipase 4-nitrophenyl butyrate substrate preincubated for 30 mins followed by substrate addition measured after 5 mins by Dixon plot method B 5.01 pKi 9800 nM Ki Bioorg Med Chem (2017) 25: 609-620 [PMID:27908755]
ChEMBL Inhibition of porcine pancreatic lipase assessed as reduction in 4-nitrophenol formation from 4-nitrophenyl butyrate substrate preincubated for 30 mins followed by substrate addition measured after 5 mins by spectrophotometer B 6 pIC50 990 nM IC50 Bioorg Med Chem (2017) 25: 609-620 [PMID:27908755]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenyl butyrate as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by spectrophotometric analysis B 6 pIC50 990 nM IC50 Bioorg Med Chem Lett (2017) 27: 3749-3754 [PMID:28705641]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenyl butyrate as substrate pretreated for 15 mins followed by substrate addition and measured after 60 mins B 6.1 pIC50 800 nM IC50 ACS Med Chem Lett (2018) 9: 662-666 [PMID:30034597]
ChEMBL Inhibition of pig pancreatic lipase assessed as p-NPB hydrolysis by ELISA B 6.22 pIC50 600 nM IC50 Bioorg. Med. Chem. Lett. (2011) 21: 1512-1514 [PMID:21282056]
ChEMBL Inhibition of pig pancreatic lipase assessed as hydrolysis of p-nitrophenylbutyrate to p-nitrophenol B 6.22 pIC50 600 nM IC50 Bioorg. Med. Chem. Lett. (2013) 23: 1099-1103 [PMID:23317572]
ChEMBL Inhibition Assay: Particularly, pancreatic lipase inhibiting activity was measured by the conventional method known to those in the art (Kim, J. H.; Kim, H. J.; Park, H. W.; Youn, S. H.; Choi, D. Y.; Shin, C. S. FEMS Microbiol. Lett. 2007, 276, 93.). To prepare the enzyme buffer, 30 mL (10 units) of pig pancreatic lipase solution (Sigma, St. Louis, Mo.) and 1 mM EDTA (pH 6.8) were mixed with 10 mM MOPS (morpholinepropanesulphonic acid), which was added to 850 mL of tris buffer (100 mM Tris-HCl and 5 mM CaCl2, pH 7.0). Then, 100 mL of the rotenoisin A or B of the present invention was mixed with the prepared enzyme buffer at the experimental concentration. As the positive control, 880 mL of Orlistat (Roche, Basel, Switzerland) was mixed with the enzyme buffer. 20 mL of substrate solution containing 20 mM p-nitrophenylbutyrate dissolved in dimethyl formaide was added to the enzyme buffer, followed by culture for 15 minutes at 37° C. that was the temperature adequate for enzyme reaction. B 6.22 pIC50 600 nM IC50 US-9328123-B2. Rotenone derivatives and a use thereof (2016)
ChEMBL Inhibition of porcine pancreatic lipase using triolein as substrate measured after 10 mins B 6.44 pIC50 360 nM IC50 ACS Med Chem Lett (2018) 9: 274-278 [PMID:29541373]
ChEMBL Inhibition of porcine pancreatic lipase pre-incubated for 15 mins before p-nitrophenylbutyrate substrate addition by microplate reader based method B 6.49 pIC50 320 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 3455-3457 [PMID:26227773]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenylbutyrate as substrate preincubated for 15 mins prior substrate addition measured after 15 mins by spectrophotometry B 6.52 pIC50 300 nM IC50 Bioorg. Med. Chem. Lett. (2012) 22: 2760-2763 [PMID:22450131]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenylbutyrate as substrate assessed as formation of p-nitrophenol preincubated for 15 mins followed by substrate addition measured after 15 mins B 6.52 pIC50 300 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 2269-2274 [PMID:25935644]
ChEMBL Inhibition of porcine pancreatic lipase using micellar solution of triolein as substrate preincubated for 5 mins before substrate addition measured after 30 mins B 6.66 pIC50 220 nM IC50 Bioorg. Med. Chem. Lett. (2012) 22: 6410-6412 [PMID:22995617]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenyl butyrate as substrate assessed as formation of p-nitrophenol preincubated for 10 mins followed by substrate addition by spectrophotometric method B 7.92 pIC50 12 nM IC50 Bioorg. Med. Chem. Lett. (2016) 26: 2788-2794 [PMID:27156775]
ChEMBL Inhibition of porcine pancreatic lipase using p-nitrophenyl butyrate as substrate after 20 mins B 8.4 pIC50 4 nM IC50 ACS Med Chem Lett (2018) 9: 274-278 [PMID:29541373]
ChEMBL Inhibition of porcine pancreatic lipase using p-NPB as substrate pretreated for 15 mins followed by substrate addition measured after 15 mins by spectrophometric method B 8.4 pIC50 4 nM IC50 J Nat Prod (2018) 81: 1098-1102 [PMID:29667821]
proteasome 20S subunit beta 1/Proteasome component C5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4208] [GtoPdb: 2404] [UniProtKB: P20618]
ChEMBL Inhibition of caspase-like activity of 20S proteasome in human red blood cells pretreated for 20 mins followed by Z-Leu-Leu-Glu-AMC substrate addition by fluorescence assay B 4 pIC50 >100000 nM IC50 Bioorg Med Chem (2017) 25: 2901-2916 [PMID:28236510]
proteasome 20S subunit beta 2/Proteasome Macropain subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3492] [GtoPdb: 2405] [UniProtKB: P49721]
ChEMBL Inhibition of trypsin-like activity of 20S proteasome in human red blood cells pretreated for 20 mins followed by Boc-Leu-Arg-Arg-AMC substrate addition by fluorescence assay B 4 pIC50 >100000 nM IC50 Bioorg Med Chem (2017) 25: 2901-2916 [PMID:28236510]
proteasome 20S subunit beta 5/Proteasome Macropain subunit MB1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4662] [GtoPdb: 2406] [UniProtKB: P28074]
ChEMBL Inhibition of chymotrypsin-like activity of 20S proteasome in human red blood cells pretreated for 20 mins followed by Suc-Leu-Leu-Val-Tyr-AMC substrate addition by fluorescence assay B 4 pIC50 >100000 nM IC50 Bioorg Med Chem (2017) 25: 2901-2916 [PMID:28236510]
Protein BAT5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6168] [UniProtKB: O95870]
ChEMBL Inhibition of recombinant BAT5 transfected in HEK293T cells by SDS-PAGE using rhodamine-tagged FP probe B 7.52 pIC50 30 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
Diacylglycerol lipase α/Sn1-specific diacylglycerol lipase alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5545] [GtoPdb: 1396] [UniProtKB: Q9Y4D2]
ChEMBL Inhibition of human recombinant DAGL-alpha-mediated sn-1-[14C]oleoyl-2-arachidonoyl-glycerol hydrolysis to 2-AG overexpressed in african green monkey COS7 cell membrane by scintillation counting B 6 pIC50 1000 nM IC50 J. Med. Chem. (2008) 51: 6970-6979 [PMID:18831576]
GtoPdb - - 7.2 pIC50 60 nM IC50 J. Cell Biol. (2003) 163: 463-8 [PMID:14610053]
ChEMBL Inhibition of human recombinant DAGLalpha overexpressed in african green monkey COS7 cells B 7.22 pIC50 60 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]
ChEMBL Inhibition of human DAGLalpha expressed in HEK293T cell membrane using [14C]SAG substrate in detergent free solution by FRET assay B 8 pIC50 10 nM IC50 Bioorg. Med. Chem. Lett. (2012) 22: 4585-4592 [PMID:22738638]
ChEMBL Inhibition of human recombinant DAGLalpha expressed in African green monkey COS cells using sn-1-stearoyl-2-[14C]-arachidonoyl-glycerol as substrate incubated for 15 mins by beta counting analysis B 9 pIC50 1 nM IC50 Bioorg. Med. Chem. (2016) 24: 1455-1468 [PMID:26917221]
Diacylglycerol lipase α/Sn1-specific diacylglycerol lipase alpha in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5180] [GtoPdb: 1396] [UniProtKB: Q6WQJ1]
ChEMBL Inhibition of DAGLalpha in mouse N18TG2 cells assessed as inhibition of ionomycin-induced formation of 2-AG incubated for 20 mins by LC-MS analysis B 7.7 pIC50 20 nM IC50 Bioorg. Med. Chem. (2016) 24: 1455-1468 [PMID:26917221]
Diacylglycerol lipase β/Sn1-specific diacylglycerol lipase beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5521] [GtoPdb: 1397] [UniProtKB: Q8NCG7]
ChEMBL Inhibition of DAGLbeta (unknown origin) B 6.38 pIC50 420 nM IC50 Bioorg. Med. Chem. (2016) 24: 1455-1468 [PMID:26917221]
GtoPdb - - 7 pIC50 100 nM IC50 J. Cell Biol. (2003) 163: 463-8 [PMID:14610053]
ChEMBL Inhibition of human recombinant DAGLbeta overexpressed in african green monkey COS7 cells B 7.22 pIC50 60 nM IC50 Bioorg. Med. Chem. Lett. (2008) 18: 5838-5841 [PMID:18657971]

ChEMBL data shown on this page come from version 27:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]