rucaparib [Ligand Id: 7736] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL1173055 (Rucaparib, AG-014699, AG-14447)
  • poly(ADP-ribose) polymerase 1/Poly [ADP-ribose] polymerase-1 in Human [ChEMBL: CHEMBL3105] [GtoPdb: 2771] [UniProtKB: P09874]
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  • poly(ADP-ribose) polymerase 2/Poly [ADP-ribose] polymerase 2 in Human [ChEMBL: CHEMBL5366] [GtoPdb: 2772] [UniProtKB: Q9UGN5]
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  • poly (ADP-ribose) polymerase 3/Poly [ADP-ribose] polymerase 3 in Human [ChEMBL: CHEMBL5083] [GtoPdb: 2864] [UniProtKB: Q9Y6F1]
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  • Poly [ADP-ribose] polymerase 4 in Human [ChEMBL: CHEMBL6142] [UniProtKB: Q9UKK3]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
poly(ADP-ribose) polymerase 1/Poly [ADP-ribose] polymerase-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3105] [GtoPdb: 2771] [UniProtKB: P09874]
GtoPdb - - 8.85 pKi 1.4 nM Ki Mol Cancer Ther (2007) 6: 945-56 [PMID:17363489]
ChEMBL Inhibition of human full-length recombinant ARTD1 assessed as incorporation of [32P]NAD+ by scintillation counting analysis B 8.85 pKi 1.4 nM Ki ACS Med. Chem. Lett. (2014) 5: 18-22 [PMID:24900770]
ChEMBL Inhibition of PARP1 (unknown origin) B 8.85 pKi 1.4 nM Ki Bioorg Med Chem Lett (2016) 26: 4127-4132 [PMID:27353531]
ChEMBL Inhibition of human recombinant full-length PARP1 after 4 mins by [32P]NAD+ incorporation assay B 8.85 pKi 1.4 nM Ki Eur J Med Chem (2019) 165: 198-215 [PMID:30684797]
ChEMBL Inhibition of PARP1 (unknown origin) B 6.3 pIC50 >500 nM IC50 J. Med. Chem. (2015) 58: 3302-3314 [PMID:25761096]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 C-3-zinc finger domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.52 pIC50 302 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 C-3-zinc finger domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.52 pIC50 301 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.4 pIC50 39.81 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP1 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.4 pIC50 39.8 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human GST-tagged PARP1 expressed in Escherichia coli using damaged DNA as substrate after 30 mins in presence of NAD+ by resazurin dye based fluorescence assay B 7.59 pIC50 26 nM IC50 Bioorg Med Chem Lett (2018) 28: 847-852 [PMID:29456106]
ChEMBL Inhibition of human recombinant full-length PARP1 after 4 mins by [32P]NAD+ incorporation assay B 8.33 pIC50 4.7 nM IC50 Eur J Med Chem (2019) 165: 198-215 [PMID:30684797]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP1 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 8.49 pIC50 3.2 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP1 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 8.5 pIC50 3.16 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human PARP1 using [3H]NAD as substrate after 1 min by microplate scintillation counting analysis B 8.7 pIC50 1.98 nM IC50 J. Med. Chem. (2016) 59: 335-357 [PMID:26652717]
ChEMBL Inhibition of PARP1 (unknown origin) B 9.09 pIC50 0.8 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 4557-4561 [PMID:26342868]
ChEMBL Inhibition of PARP1 (unknown origin) B 9.1 pIC50 0.8 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
Poly [ADP-ribose] polymerase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2429708] [UniProtKB: Q53GL7]
ChEMBL Inhibition of recombinant human His6-tagged PARP10 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.64 pIC50 2310 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP10 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.64 pIC50 2290.87 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP10 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.24 pIC50 570 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP10 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.25 pIC50 562.34 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 12 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2429709] [UniProtKB: Q9H0J9]
ChEMBL Inhibition of recombinant human His6-tagged PARP12 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.18 pIC50 6650 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP12 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.18 pIC50 6606.93 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 14 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2176777] [UniProtKB: Q460N5]
ChEMBL Inhibition of recombinant human His6-tagged PARP14 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.05 pIC50 8912.51 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP14 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 5.05 pIC50 8830 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 15 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2176778] [UniProtKB: Q460N3]
ChEMBL Inhibition of recombinant human His6-tagged PARP15 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 4.49 pIC50 32600 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP15 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 4.49 pIC50 32359.37 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
poly(ADP-ribose) polymerase 2/Poly [ADP-ribose] polymerase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5366] [GtoPdb: 2772] [UniProtKB: Q9UGN5]
ChEMBL Inhibition of recombinant human His6-tagged PARP2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.86 pIC50 138.04 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.86 pIC50 138 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP2 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.55 pIC50 28.2 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP2 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 7.55 pIC50 28.18 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of PARP2 (unknown origin) B 9.3 pIC50 0.5 nM IC50 J Med Chem (2019) 62: 5330-5357 [PMID:31042381]
poly (ADP-ribose) polymerase 3/Poly [ADP-ribose] polymerase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5083] [GtoPdb: 2864] [UniProtKB: Q9Y6F1]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP3 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.29 pIC50 512 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of full length recombinant human His6-tagged PARP3 expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.39 pIC50 407.38 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
Poly [ADP-ribose] polymerase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6142] [UniProtKB: Q9UKK3]
ChEMBL Inhibition of recombinant human His6-tagged PARP4 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.08 pIC50 839 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human His6-tagged PARP4 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.08 pIC50 831.76 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
tankyrase/Tankyrase-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6164] [GtoPdb: 3108] [UniProtKB: O95271]
ChEMBL Inhibition of recombinant human TNKS1 ADP-ribosyltransferase/sterile alpha motif domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.84 pIC50 144.54 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human TNKS1 ADP-ribosyltransferase/sterile alpha motif domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.84 pIC50 144 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human 6xhis-tagged ARTD5 (1030 to 1317) expressed in Escherichia coli Rosetta2 (DE3) cells using NAD+ as substrate by fluorescence assay B 7.6 pIC50 25.12 nM IC50 ACS Med. Chem. Lett. (2014) 5: 18-22 [PMID:24900770]
ChEMBL Inhibition of human 6xhis-tagged ARTD5 (1030 to 1317) expressed in Escherichia coli Rosetta2 (DE3) cells using NAD+ as substrate by fluorescence assay B 7.6 pIC50 25 nM IC50 ACS Med. Chem. Lett. (2014) 5: 18-22 [PMID:24900770]
tankyrase 2/Tankyrase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6154] [GtoPdb: 3109] [UniProtKB: Q9H2K2]
ChEMBL Inhibition of recombinant human TNKS2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.05 pIC50 891.25 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of recombinant human TNKS2 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay B 6.05 pIC50 890 nM IC50 J Med Chem (2017) 60: 1262-1271 [PMID:28001384]
ChEMBL Inhibition of human N-terminal 6xhis-tagged ARTD6 (873 to 1161) expressed in Escherichia coli Rosetta2 (DE3) cells using NAD+ as substrate by fluorescence assay B 7.85 pIC50 14.13 nM IC50 ACS Med. Chem. Lett. (2014) 5: 18-22 [PMID:24900770]
ChEMBL Inhibition of human N-terminal 6xhis-tagged ARTD6 (873 to 1161) expressed in Escherichia coli Rosetta2 (DE3) cells using NAD+ as substrate by fluorescence assay B 7.85 pIC50 14 nM IC50 ACS Med. Chem. Lett. (2014) 5: 18-22 [PMID:24900770]

ChEMBL data shown on this page come from version 28:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]