adenosine 5'-monophosphate [Ligand Id: 2455] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL752 (My-B-Den, 5'-adenylic acid, A-5MP, Adenyl, Adenosine Phosphate, NSC-20264, Adenosine Monophosphate, Vitamin B8)
  • 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 in Human [ChEMBL: CHEMBL3351218] [UniProtKB: O43598]
  • 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 in Rat [ChEMBL: CHEMBL3329079] [UniProtKB: O35820]
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  • A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
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  • protein kinase AMP-activated non-catalytic subunit beta 2/protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit gamma 3/protein kinase AMP-activated non-catalytic subunit gamma 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase, AMPK in Human [ChEMBL: CHEMBL2096907] [GtoPdb: 1544154515421541154715461543] [UniProtKB: O43741P54619P54646Q13131Q9UGI9Q9UGJ0Q9Y478]
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  • protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 in Human [ChEMBL: CHEMBL2111345] [GtoPdb: 154515411543] [UniProtKB: P54619Q13131Q9Y478]
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  • Human immunodeficiency virus type 1 protease in Human immunodeficiency virus 1 [ChEMBL: CHEMBL243] [UniProtKB: Q72874]
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  • Ketopantoate reductase in Escherichia coli K-12 [ChEMBL: CHEMBL4855] [UniProtKB: P0A9J4]
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  • L-lactate dehydrogenase A chain in Human [ChEMBL: CHEMBL4835] [UniProtKB: P00338]
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  • P2Y1 receptor/Purinergic receptor P2Y1 in Human [ChEMBL: CHEMBL4315] [GtoPdb: 323] [UniProtKB: P47900]
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  • P2Y11 receptor/Purinergic receptor P2Y11 in Human [ChEMBL: CHEMBL4867] [GtoPdb: 327] [UniProtKB: Q96G91]
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  • P2Y2 receptor/Purinergic receptor P2Y2 in Human [ChEMBL: CHEMBL4398] [GtoPdb: 324] [UniProtKB: P41231]
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  • TRPM2/Transient receptor potential cation channel subfamily M member 2 in Human [ChEMBL: CHEMBL1250402] [GtoPdb: 494] [UniProtKB: O94759]
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  • SRC proto-oncogene, non-receptor tyrosine kinase/Tyrosine-protein kinase SRC in Human [ChEMBL: CHEMBL267] [GtoPdb: 2206] [UniProtKB: P12931]
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  • TRPM4 in Human [GtoPdb: 496] [UniProtKB: Q8TD43]
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  • AMP kinase in Human [GtoPdb: 1540]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
2'-deoxynucleoside 5'-phosphate N-hydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3351218] [UniProtKB: O43598]
ChEMBL Inhibition of human DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically B 4.72 pKi 19200 nM Ki Eur. J. Med. Chem. (2014) 85: 418-437 [PMID:25108359]
2'-deoxynucleoside 5'-phosphate N-hydrolase 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3329079] [UniProtKB: O35820]
ChEMBL Inhibition of rat DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically B 4.4 pKi 40000 nM Ki Eur. J. Med. Chem. (2014) 85: 418-437 [PMID:25108359]
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
ChEMBL Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay F 6.3 pEC50 500 nM EC50 J. Med. Chem. (2012) 55: 6467-6477 [PMID:22738238]
Adenylate kinase 2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2376] [UniProtKB: P29410]
ChEMBL Inhibitory activity against rat adenylate kinase II was determined in the presence of AMP B 4.1 pKi 80000 nM Ki J. Med. Chem. (1982) 25: 1179-1184 [PMID:6292418]
protein kinase AMP-activated non-catalytic subunit beta 2/protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit gamma 3/protein kinase AMP-activated non-catalytic subunit gamma 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase, AMPK in Human (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2096907] [GtoPdb: 1544154515421541154715461543] [UniProtKB: O43741P54619P54646Q13131Q9UGI9Q9UGJ0Q9Y478]
ChEMBL Activation of human AMPK after 15 mins B 5.23 pEC50 5900 nM EC50 ACS Med. Chem. Lett. (2010) 1: 478-482 [PMID:24900234]
protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111345] [GtoPdb: 154515411543] [UniProtKB: P54619Q13131Q9Y478]
ChEMBL Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 by SPR binding assay B 5.43 pKd 3700 nM Kd J Med Chem (2016) 59: 8068-8081 [PMID:27490827]
ChEMBL Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay B 5.82 pEC50 1519 nM EC50 J Med Chem (2016) 59: 8068-8081 [PMID:27490827]
Fructose-1,6-bisphosphatase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3975] [UniProtKB: P09467]
ChEMBL Inhibition of human liver fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay B 5.01 pIC50 9800 nM IC50 Eur. J. Med. Chem. (2010) 45: 1478-1484 [PMID:20116906]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli BL21(DE3) Rosetta cells assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupling based spectrophotometry B 5.49 pIC50 3200 nM IC50 Eur. J. Med. Chem. (2015) 90: 394-405 [PMID:25461330]
ChEMBL Inhibition of human recombinant FBPase expressed in Escherichia coli BL21(DE3) by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupled assay B 5.49 pIC50 3200 nM IC50 Bioorg. Med. Chem. (2014) 22: 1850-1862 [PMID:24530031]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli by spectrophotometry B 6 pIC50 1000 nM IC50 J. Med. Chem. (2009) 52: 2880-2898 [PMID:19348494]
ChEMBL Inhibition of FBPase (unknown origin) B 6 pIC50 1000 nM IC50 MedChemComm (2011) 2: 287-290
ChEMBL Concentration required to inhibit the human liver recombinant fructose-1,6-bisphosphatase. B 6.1 pIC50 800 nM IC50 Bioorg. Med. Chem. Lett. (2001) 11: 17-21 [PMID:11140724]
ChEMBL Inhibition of FBPase in human liver B 6.36 pIC50 440 nM IC50 Bioorg. Med. Chem. Lett. (2010) 20: 594-599 [PMID:19969452]
ChEMBL Inhibition of FBPase in human liver using fructose-2,6phosphate as a substrate B 6.85 pIC50 140 nM IC50 Bioorg. Med. Chem. Lett. (2010) 20: 594-599 [PMID:19969452]
Fructose-1,6-bisphosphatase in Pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2263] [UniProtKB: P00636]
ChEMBL Inhibition of pig FBPase expressed in Escherichia coli EK1601 by spectrophotometry B 5.89 pIC50 1300 nM IC50 Bioorg. Med. Chem. (2009) 17: 3916-3922 [PMID:19419876]
ChEMBL Inhibition of pig kidney fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay B 5.89 pIC50 1300 nM IC50 Eur. J. Med. Chem. (2010) 45: 1478-1484 [PMID:20116906]
Human immunodeficiency virus type 1 protease in Human immunodeficiency virus 1 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL243] [UniProtKB: Q72874]
ChEMBL Equilibrium constant for the interaction between inhibitor and HIV-1 Protease B 8.95 pKd 1.13 nM Kd J. Med. Chem. (2002) 45: 5430-5439 [PMID:12459011]
ChEMBL Inhibition constant against HIV-1 Protease B 9.64 pKi 0.23 nM Ki J. Med. Chem. (2002) 45: 5430-5439 [PMID:12459011]
Ketopantoate reductase in Escherichia coli K-12 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4855] [UniProtKB: P0A9J4]
ChEMBL Binding affinity to Escherichia coli KPR B 2.3 pKd >5000000 nM Kd J. Med. Chem. (2006) 49: 4992-5000 [PMID:16884311]
ChEMBL Inhibition of Escherichia coli KPR B 2.2 pKi 6300000 nM Ki J. Med. Chem. (2006) 49: 4992-5000 [PMID:16884311]
L-lactate dehydrogenase A chain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4835] [UniProtKB: P00338]
ChEMBL Binding affinity to 6-His-tagged human LDHA (2 to 322 amino acid residue) expressed in Escherichia coli BL21 (DE3) cells by NMR analysis in presence of NADH B 3.19 pKd 650000 nM Kd J. Med. Chem. (2012) 55: 3285-3306 [PMID:22417091]
ChEMBL Inhibition of full length human recombinant LDHA expressed in Escherichia coli BL21 (DE3) cells after 10 mins by spectrophotometric analysis B 3.3 pIC50 >500000 nM IC50 J. Med. Chem. (2012) 55: 3285-3306 [PMID:22417091]
P2Y purinoceptor 1 in Turkey (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5720] [UniProtKB: P49652]
ChEMBL Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell F 5.46 pEC50 3500 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell F 5.55 pEC50 2800 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
P2Y1 receptor/Purinergic receptor P2Y1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4315] [GtoPdb: 323] [UniProtKB: P47900]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell F 5.14 pEC50 7200 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell F 5.82 pEC50 1500 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
P2Y11 receptor/Purinergic receptor P2Y11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4867] [GtoPdb: 327] [UniProtKB: Q96G91]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 11 (hP2Y11) stably expressed in 131N1 astrocytoma cell at 10 uM F 4.76 pEC50 17250 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
P2Y2 receptor/Purinergic receptor P2Y2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4398] [GtoPdb: 324] [UniProtKB: P41231]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell F 5.43 pEC50 3700 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell F 7.07 pEC50 85 nM EC50 J. Med. Chem. (2002) 45: 208-218 [PMID:11754592]
TRPM2/Transient receptor potential cation channel subfamily M member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1250402] [GtoPdb: 494] [UniProtKB: O94759]
ChEMBL Inhibition of TRPM2 (unknown origin) B 4.15 pIC50 70000 nM IC50 J. Med. Chem. (2013) 56: 10079-10102 [PMID:24304219]
ChEMBL Inhibition of human TRPM2 assessed as reduction in ADPR-induced channel currents by whole cell patch clamp electrophysiology method B 4.15 pIC50 70000 nM IC50 Eur J Med Chem (2018) 152: 235-252 [PMID:29723786]
SRC proto-oncogene, non-receptor tyrosine kinase/Tyrosine-protein kinase SRC in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL267] [GtoPdb: 2206] [UniProtKB: P12931]
ChEMBL Inhibition of p60c-src expressed in chick embryo fibroblast B 7 pIC50 100 nM IC50 J. Nat. Prod. (1992) 55: 1529-1560 [PMID:1479375]
TRPM4 in Human [GtoPdb: 496] [UniProtKB: Q8TD43]
GtoPdb - - 4.7 pIC50 - - - Pflugers Arch. (2004) 448: 70-5 [PMID:14758478]
AMP kinase in Human [GtoPdb: 1540]
GtoPdb Activation of AMPK partially purified from HEK cells. - 4.21 pEC50 61000 nM EC50 Cell Metab. (2006) 3: 403-16 [PMID:16753576]

ChEMBL data shown on this page come from version 27:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]