abemaciclib [Ligand Id: 7382] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL3301610 (Verzenio, Abemaciclib, LY-2835219, Verzenios)
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  • cyclin dependent kinase 1/Cyclin-dependent kinase 1 in Human [ChEMBL: CHEMBL308] [GtoPdb: 1961] [UniProtKB: P06493]
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  • cyclin dependent kinase 4/Cyclin-dependent kinase 4 in Human [ChEMBL: CHEMBL331] [GtoPdb: 1976] [UniProtKB: P11802]
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  • cyclin dependent kinase 4/Cyclin-dependent kinase 4/G1/S-specific cyclin-E1 in Human [ChEMBL: CHEMBL3885554] [GtoPdb: 1976] [UniProtKB: P11802P24864]
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  • cyclin dependent kinase 5/Cyclin-dependent kinase 5/CDK5 activator 1 in Human [ChEMBL: CHEMBL1907600] [GtoPdb: 1977] [UniProtKB: Q00535Q15078]
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  • cyclin dependent kinase 6/Cyclin-dependent kinase 6 in Human [ChEMBL: CHEMBL2508] [GtoPdb: 1978] [UniProtKB: Q00534]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
cyclin dependent kinase 6/CDK6/cyclin D1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111455] [GtoPdb: 1978] [UniProtKB: P24385Q00534]
ChEMBL Inhibition of recombinant full length human N-terminal GST-tagged CDK6 (1 to 326 residues)/cyclin D1 (4 to 295 residues) expressed in sf9 cells using C-terminal retinoblastoma fragment as substrate after 90 mins by [gamma-33P]ATP based microbeta scintillation counting analysis B 8 pKi 10 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
GtoPdb CDK6/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8 pIC50 10 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
ChEMBL Inhibition of recombinant human N-terminal GST-tagged CDK4 (M1 to A326 residues)/Cyclin D1 (Q4 to I295 residues) expressed in sf9 cells using Rb protein (773 to 928 residues) as substrate in presence of [33P]-ATP by scintillation counting method B 8 pIC50 10 nM IC50 Bioorg Med Chem Lett (2018) 28: 974-978 [PMID:29429832]
cyclin dependent kinase 6/CDK6/cyclin D3 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111448] [GtoPdb: 1978] [UniProtKB: P30281Q00534]
ChEMBL Competitive inhibition of CDK6/cyclin D3 (unknown origin) assessed as phosphorylation of CTRF after 50 mins by Michaelis-Menten plot analysis in presence of ATP B 8.09 pKi 8.2 nM Ki Bioorg. Med. Chem. Lett. (2015) 25: 3420-3435 [PMID:26115571]
GtoPdb CDK6/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8 pIC50 10 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
ChEMBL Inhibition of human CDK6/Cyclin-D3 B 8 pIC50 10 nM IC50 Eur J Med Chem (2018) 148: 140-153 [PMID:29459274]
ChEMBL Inhibition of CDK6/Cyclin-D3 (unknown origin) using histoneH1 as substrate after 90 mins by ADP-Glo assay B 8.3 pIC50 5 nM IC50 Eur J Med Chem (2018) 144: 1-28 [PMID:29247857]
cyclin dependent kinase 7/CDK7/Cyclin H/MNAT1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038473] [GtoPdb: 1979] [UniProtKB: P50613P51946P51948]
ChEMBL Inhibition of recombinant human full length C-terminal His6-tagged CDK7/cyclin H/N-terminal GST-tagged MAT1 expressed in baculovirus infected Sf21 insect cells using cdk7 substrate peptide B 5.41 pKi 3910 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
ChEMBL Inhibition of CDK7/Cyclin H/MAT1 (unknown origin) using (YSPTSPS)2KK peptide as substrate in presence of [gamma-33P]ATP B 5.51 pIC50 3112 nM IC50 Eur. J. Med. Chem. (2016) 108: 701-719 [PMID:26741853]
cyclin dependent kinase 9/CDK9/cyclin T1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111389] [GtoPdb: 1981] [UniProtKB: O60563P50750]
ChEMBL Inhibition of CDK9/cyclin T (unknown origin) B 7.24 pKi 57 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
ChEMBL Inhibition of CDK9/Cyclin T1 (unknown origin) using (YSPTSPS)2KK peptide as substrate in presence of [gamma-33P]ATP B 7 pIC50 101 nM IC50 Eur. J. Med. Chem. (2016) 108: 701-719 [PMID:26741853]
ChEMBL Inhibition of human CDK9/Cyclin-T1 B 7.24 pIC50 57 nM IC50 Eur J Med Chem (2018) 148: 140-153 [PMID:29459274]
cyclin dependent kinase 1/Cyclin-dependent kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL308] [GtoPdb: 1961] [UniProtKB: P06493]
ChEMBL Inhibition of CDK1 (unknown origin) using U-light as substrate preincubated for 1 hr followed by EDTA addition measured after 1 hr by LANCE Ultra kinase assay B 5.82 pIC50 1523 nM IC50 Bioorg Med Chem Lett (2017) 27: 5332-5336 [PMID:29074254]
cyclin dependent kinase 1/Cyclin-dependent kinase 1/cyclin B1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907602] [GtoPdb: 1961] [UniProtKB: P06493P14635]
ChEMBL Inhibition of CDK1/cyclin B (unknown origin) B 5.79 pKi 1627 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
ChEMBL Inhibition of human CDK1/cyclin B1 (unknown origin) B 5.79 pIC50 1627 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 3420-3435 [PMID:26115571]
ChEMBL Inhibition of CDK1/Cyclin B (unknown origin) expressed in baculoviral infected insect Sf9 cells using histone H1 as substrate in presence of [gamma-33P]ATP B 6.43 pIC50 371 nM IC50 Eur. J. Med. Chem. (2016) 108: 701-719 [PMID:26741853]
cyclin dependent kinase 2/Cyclin-dependent kinase 2/cyclin E1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907605] [GtoPdb: 1973] [UniProtKB: P24864P24941]
ChEMBL Inhibition of human CDK2/cyclin E B 6.3 pKi 504 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
cyclin dependent kinase 4/Cyclin-dependent kinase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL331] [GtoPdb: 1976] [UniProtKB: P11802]
ChEMBL Inhibition of CDK4 (unknown origin) B 8.7 pIC50 2 nM IC50 Eur J Med Chem (2017) 142: 424-458 [PMID:28911822]
GtoPdb CDK4/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8.7 pIC50 2 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
ChEMBL Inhibition of CDK4 (unknown origin) using U-light as substrate preincubated for 1 hr followed by EDTA addition measured after 1 hr by LANCE Ultra kinase assay B 8.72 pIC50 1.9 nM IC50 Bioorg Med Chem Lett (2017) 27: 5332-5336 [PMID:29074254]
ChEMBL Inhibition of CDK4 (unknown origin) after 90 mins by ADP-Glo assay B 8.77 pIC50 1.7 nM IC50 Bioorg Med Chem Lett (2018) 28: 974-978 [PMID:29429832]
cyclin dependent kinase 4/Cyclin-dependent kinase 4/cyclin D1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907601] [GtoPdb: 1976] [UniProtKB: P11802P24385]
ChEMBL Inhibition of recombinant human N-terminal GST-tagged CDK4 (4 to 303 residues)/cyclin D1 (4 to 295 residues) expressed in sf9 cells using C-terminal retinoblastoma fragment as substrate after 90 mins by [gamma-33P]ATP based microbeta scintillation counting analysis B 8.7 pKi 2 nM Ki J Med Chem (2016) 59: 8667-8684 [PMID:27171036]
ChEMBL Competitive inhibition of human CDK4/cyclin D1 expressed in insect cells assessed as phosphorylation of CTRF after 50 mins by Michaelis-Menten plot analysis in presence of ATP B 9.22 pKi 0.6 nM Ki Bioorg. Med. Chem. Lett. (2015) 25: 3420-3435 [PMID:26115571]
ChEMBL Inhibition of CDK4/Cyclin D1 (unknown origin) using RPPTLSPIPHIPR peptide as substrate in presence of [gamma-33P]ATP B 8.3 pIC50 5 nM IC50 Eur. J. Med. Chem. (2016) 108: 701-719 [PMID:26741853]
ChEMBL Inhibition of human CDK4/cyclin D1 expressed in insect cells assessed as phosphorylation of CTRF after 50 mins by microplate scintillation counter B 8.7 pIC50 2 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 3420-3435 [PMID:26115571]
ChEMBL Inhibition of recombinant human N-terminal GST-tagged CDK4 (S4 to E303 residues)/Cyclin D1 (Q4 to I295 residues) expressed in sf9 cells using Rb protein (773 to 928 residues) as substrate in presence of [33P]-ATP by scintillation counting method B 8.7 pIC50 2 nM IC50 Bioorg Med Chem Lett (2018) 28: 974-978 [PMID:29429832]
GtoPdb CDK4/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8.7 pIC50 2 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
cyclin dependent kinase 4/Cyclin-dependent kinase 4/G1/S-specific cyclin-E1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3885554] [GtoPdb: 1976] [UniProtKB: P11802P24864]
ChEMBL Inhibition of human CDK4/cyclin D1 using ULight-substrate after 1 hr by LANCE assay B 8.7 pIC50 2 nM IC50 Eur J Med Chem (2018) 148: 140-153 [PMID:29459274]
GtoPdb CDK4/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8.7 pIC50 2 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
cyclin dependent kinase 5/Cyclin-dependent kinase 5/CDK5 activator 1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907600] [GtoPdb: 1977] [UniProtKB: Q00535Q15078]
ChEMBL Inhibition of CDK5/p35NCK (unknown origin) using histone H1 as substrate in presence of [gamma-33P]ATP B 6.39 pIC50 405 nM IC50 Eur. J. Med. Chem. (2016) 108: 701-719 [PMID:26741853]
cyclin dependent kinase 6/Cyclin-dependent kinase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2508] [GtoPdb: 1978] [UniProtKB: Q00534]
GtoPdb CDK6/cyclin D1 complex expressed in and purified from insect cells. Assays used the methanesulfonate salt of the compound. - 8 pIC50 10 nM IC50 Invest New Drugs (2014) 32: 825-37 [PMID:24919854]
ChEMBL Inhibition of CDK6 (unknown origin) B 8 pIC50 10 nM IC50 Eur J Med Chem (2017) 142: 424-458 [PMID:28911822]
ChEMBL Inhibition of recombinant human full length CDK6 expressed in baculovirus infected Sf9 insect cells using histone H1 substrate after 90 mins by ADP-Glo assay B 8.11 pIC50 7.8 nM IC50 Bioorg Med Chem Lett (2018) 28: 974-978 [PMID:29429832]
ChEMBL Inhibition of CDK6 (unknown origin) using U-light as substrate preincubated for 1 hr followed by EDTA addition measured after 1 hr by LANCE Ultra kinase assay B 8.72 pIC50 1.9 nM IC50 Bioorg Med Chem Lett (2017) 27: 5332-5336 [PMID:29074254]
Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL Inhibition of human ERG B 4.96 pIC50 10900 nM IC50 Bioorg Med Chem Lett (2018) 28: 974-978 [PMID:29429832]

ChEMBL data shown on this page come from version 27:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]