idelalisib [Ligand Id: 6741] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL2216870 (GS-11CAL-101, CAL-101, Zydelig, Idelalisib, GS-1101)
  • protein kinase, DNA-activated, catalytic subunit/DNA-dependent protein kinase in Human [ChEMBL: CHEMBL3142] [GtoPdb: 2800] [UniProtKB: P78527]
  • This target only has 0 pki data point
  • 0
1 CHEMBL2216870_lig_chart_1 DNA-dependent protein kinase Human
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  • phosphatidylinositol 3-kinase catalytic subunit type 3/Phosphatidylinositol 3-kinase catalytic subunit type 3 in Human [ChEMBL: CHEMBL1075165] [GtoPdb: 2152] [UniProtKB: Q8NEB9]
  • This target only has 0 pki data point
  • 0
2 CHEMBL2216870_lig_chart_2 Phosphatidylinositol 3-kinase catalytic subunit type 3 Human
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  • phosphoinositide-3-kinase regulatory subunit 2/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/Phosphatidylinositol 3-kinase regulatory subunit beta/Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform in Human [ChEMBL: CHEMBL3885615] [GtoPdb: 25042154] [UniProtKB: O00459P42338]
  • This target only has 0 pki data point
  • 0
3 CHEMBL2216870_lig_chart_3 Phosphatidylinositol 3-kinase regulatory subunit beta/Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform Human
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  • phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta/Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide in Human [ChEMBL: CHEMBL5554] [GtoPdb: 2151] [UniProtKB: O00750]
  • This target only has 0 pki data point
  • 0
4 CHEMBL2216870_lig_chart_4 Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide Human
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  • phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha/Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha in Human [ChEMBL: CHEMBL1075102] [GtoPdb: 2150] [UniProtKB: O00443]
  • This target only has 0 pki data point
  • 0
5 CHEMBL2216870_lig_chart_5 Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha Human
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  • phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha/p85-alpha in Human [ChEMBL: CHEMBL2111367] [GtoPdb: 25032153] [UniProtKB: P27986P42336]
  • This target only has 0 pki data point
  • 0
6 CHEMBL2216870_lig_chart_6 PI3-kinase p110-alpha/p85-alpha Human
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  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha subunit in Human [ChEMBL: CHEMBL4005] [GtoPdb: 2153] [UniProtKB: P42336]
  • This target only has 0 pki data point
  • 0
7 CHEMBL2216870_lig_chart_7 PI3-kinase p110-alpha subunit Human
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  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3-kinase p110-beta subunit in Human [ChEMBL: CHEMBL3145] [GtoPdb: 2154] [UniProtKB: P42338]
  • This target only has 0 pki data point
  • 0
8 CHEMBL2216870_lig_chart_8 PI3-kinase p110-beta subunit Human
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  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta/phosphoinositide-3-kinase regulatory subunit 1/PI3-kinase p110-delta/p85-alpha in Human [ChEMBL: CHEMBL2111432] [GtoPdb: 21552503] [UniProtKB: O00329P27986]
  • This target only has 0 pki data point
  • 0
9 CHEMBL2216870_lig_chart_9 PI3-kinase p110-delta/p85-alpha Human
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  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta/PI3-kinase p110-delta subunit in Human [ChEMBL: CHEMBL3130] [GtoPdb: 2155] [UniProtKB: O00329]
  • This target only has 0 pki data point
  • 0
10 CHEMBL2216870_lig_chart_10 PI3-kinase p110-delta subunit Human
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  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma/PI3-kinase p110-gamma subunit in Human [ChEMBL: CHEMBL3267] [GtoPdb: 2156] [UniProtKB: P48736]
  • This target only has 0 pki data point
  • 0
11 CHEMBL2216870_lig_chart_11 PI3-kinase p110-gamma subunit Human
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  • phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3K p110 beta/p85 alpha in Human [ChEMBL: CHEMBL3038510] [GtoPdb: 25032154] [UniProtKB: P27986P42338]
  • This target only has 0 pki data point
  • 0
12 CHEMBL2216870_lig_chart_12 PI3K p110 beta/p85 alpha Human
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  • phosphatidylinositol 4-kinase alpha/PI4-kinase alpha subunit in Human [ChEMBL: CHEMBL3667] [GtoPdb: 2148] [UniProtKB: P42356]
  • This target only has 0 pki data point
  • 0
13 CHEMBL2216870_lig_chart_13 PI4-kinase alpha subunit Human
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  • phosphatidylinositol 4-kinase beta/PI4-kinase beta subunit in Human [ChEMBL: CHEMBL3268] [GtoPdb: 2149] [UniProtKB: Q9UBF8]
  • This target only has 0 pki data point
  • 0
14 CHEMBL2216870_lig_chart_14 PI4-kinase beta subunit Human
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  • mechanistic target of rapamycin kinase/Serine/threonine-protein kinase mTOR in Human [ChEMBL: CHEMBL2842] [GtoPdb: 2109] [UniProtKB: P42345]
  • This target only has 0 pki data point
  • 0
15 CHEMBL2216870_lig_chart_15 Serine/threonine-protein kinase mTOR Human
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
protein kinase, DNA-activated, catalytic subunit/DNA-dependent protein kinase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3142] [GtoPdb: 2800] [UniProtKB: P78527]
ChEMBL Inhibition of human DNA-PK by non-radiometric ADP-Glo assay B 5.17 pIC50 6700 nM IC50 ACS Med. Chem. Lett. (2015) 6: 3-6 [PMID:25589915]
ChEMBL Inhibition of human recombinant DNA-PK by Select-screen kinase inhibitor assay B 5.17 pIC50 6700 nM IC50 J Med Chem (2017) 60: 47-65 [PMID:27644332]
phosphatidylinositol 3-kinase catalytic subunit type 3/Phosphatidylinositol 3-kinase catalytic subunit type 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075165] [GtoPdb: 2152] [UniProtKB: Q8NEB9]
ChEMBL Inhibition of GST-tagged full length recombinant human VPS34 expressed in Baculovirus expression system using PI:PS as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 5.35 pIC50 4417 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of Vps34 (unknown origin) B 6.01 pIC50 980 nM IC50 J Med Chem (2019) 62: 4783-4814 [PMID:30582813]
phosphoinositide-3-kinase regulatory subunit 2/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/Phosphatidylinositol 3-kinase regulatory subunit beta/Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3885615] [GtoPdb: 25042154] [UniProtKB: O00459P42338]
ChEMBL Inhibition of human PI3K p110beta/p85beta by fluorescence-based immunoassay B 6.24 pIC50 579 nM IC50 MedChemComm (2015) 6: 2029-2035
GtoPdb - - 6.25 pIC50 565 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta/Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5554] [GtoPdb: 2151] [UniProtKB: O00750]
ChEMBL Inhibition of N-terminal GST-tagged recombinant human PIK3C2B catalytic domain expressed in Baculovirus expression system using PI as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 5 pIC50 >10000 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of human PI3KC2beta by non-radiometric ADP-Glo assay B 6 pIC50 >1000 nM IC50 ACS Med. Chem. Lett. (2015) 6: 3-6 [PMID:25589915]
ChEMBL Inhibition of human recombinant PI3KC2beta by Select-screen kinase inhibitor assay B 6 pIC50 >1000 nM IC50 J Med Chem (2017) 60: 47-65 [PMID:27644332]
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha/Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075102] [GtoPdb: 2150] [UniProtKB: O00443]
ChEMBL Inhibition of N-terminal GST-tagged recombinant human PIK3C2A catalytic domain expressed in Baculovirus expression system using PI as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 5 pIC50 >10000 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha/p85-alpha in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111367] [GtoPdb: 25032153] [UniProtKB: P27986P42336]
ChEMBL Inhibition of His-tagged recombinant human PIK3CA/PIK3R1 expressed in Baculovirus expression system B 5.07 pIC50 8600 nM IC50 J Med Chem (2020) 63: 441-469 [PMID:31550151]
ChEMBL Inhibition of His-tagged recombinant human full length PI3K p110alpha/p85alpha expressed in baculovirus expression system using lipid substrate incubated for 60 mins by kinase-Glo reagent based luminescence assay B 5.66 pIC50 2164 nM IC50 Eur J Med Chem (2019) 170: 112-125 [PMID:30878826]
ChEMBL Inhibition of human PI3K p110alpha/p85alpha by fluorescence-based immunoassay B 6.08 pIC50 825 nM IC50 MedChemComm (2015) 6: 2029-2035
GtoPdb - - 6.09 pIC50 820 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of human N-terminal His-tagged PI3Kalpha/p85alpha expressed in Spodoptera frugiperda using phosphatidylinositol as substrate B 6.1 pIC50 800 nM IC50 Eur J Med Chem (2019) 163: 413-427 [PMID:30530193]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4005] [GtoPdb: 2153] [UniProtKB: P42336]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 5 pIC50 10000 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 5 pIC50 10000 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kalpha (unknown origin) by biochemical Alphascreen assay B 5.01 pIC50 9700 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1104-1109 [PMID:25666823]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using PIP2 as substrate measured after 30 mins by HTRF assay B 5.78 pIC50 1650 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using PIP2 as substrate after 2 hrs by ADP-Glo assay B 6.09 pIC50 822 nM IC50 MedChemComm (2019) 10: 413-420
ChEMBL Inhibition of PI3K p110alpha (unknown origin) B 6.09 pIC50 820 nM IC50 J. Med. Chem. (2013) 56: 1922-1939 [PMID:23410005]
ChEMBL Inhibition of PI3Kalpha (unknown origin) B 6.09 pIC50 820 nM IC50 MedChemComm (2012) 3: 1337-1355
ChEMBL Inhibition of recombinant human full length His-tagged PI3Kalpha expressed in baculovirus expression system B 6.09 pIC50 820 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
GtoPdb - - 6.09 pIC50 820 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of PI3Kalpha (unknown origin) after 60 mins using fluorescein-labeled kinase tracer by HTRF assay B 6.1 pIC50 800 nM IC50 Bioorg Med Chem Lett (2017) 27: 2849-2853 [PMID:28209465]
ChEMBL Inhibition of PI3Kalpha (unknown origin) B 6.1 pIC50 800 nM IC50 J Med Chem (2017) 60: 5193-5208 [PMID:28541707]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using Biotin-S11S12 as substrate after 60 mins in presence of ATP by Kinase Glo luminescence assay B 6.13 pIC50 737 nM IC50 Eur J Med Chem (2018) 151: 9-17 [PMID:29601991]
ChEMBL Inhibition of PI3K alpha (unknown origin) using lipid substrate measured after 40 mins in presence of ATP by Kinase-Glo plus reagent based luminescence assay B 6.23 pIC50 583 nM IC50 Bioorg Med Chem (2019) 27: 114930-114930 [PMID:31176568]
ChEMBL Inhibition of PI3Kalpha (unknown origin) using PIP2:PS as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 10 uM ATP by ADP-Glo luminescence assay B 6.41 pIC50 391.3 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of PI3K p110alpha (unknown origin) using lipid substrate after 40 mins by kinase-Glo assay B 6.51 pIC50 306.4 nM IC50 Bioorg Med Chem (2018) 26: 2028-2040 [PMID:29534936]
ChEMBL Inhibition of PI3K alpha (unknown origin) using phosphatidyl inositol as substrate measured after 60 mins in presence of ATP by Kinase-Glo Plus reagent-based luminescence assay B 6.75 pIC50 177.2 nM IC50 Bioorg Med Chem (2019) 27: 115035-115035 [PMID:31434616]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3-kinase p110-beta subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3145] [GtoPdb: 2154] [UniProtKB: P42338]
ChEMBL Inhibition of His-tagged recombinant human PIK3CB B 5.4 pIC50 4000 nM IC50 J Med Chem (2020) 63: 441-469 [PMID:31550151]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 5.7 pIC50 1995.26 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 5.7 pIC50 1995.26 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kbeta (unknown origin) by biochemical Alphascreen assay B 5.85 pIC50 1400 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1104-1109 [PMID:25666823]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using PIP2:PS as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 6.07 pIC50 845.5 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using PIP2 as substrate measured after 30 mins by HTRF assay B 6.17 pIC50 669 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of human PI3Kbeta using PIP2 as substrate after 1 hr B 6.24 pIC50 571 nM IC50 Bioorg Med Chem Lett (2019) 29: 1313-1319 [PMID:30975623]
ChEMBL Inhibition of PI3Kbeta (unknown origin) B 6.25 pIC50 565 nM IC50 MedChemComm (2012) 3: 1337-1355
ChEMBL Inhibition of recombinant human full length His-tagged PI3Kbeta expressed in insect cells B 6.25 pIC50 565 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
GtoPdb - - 6.25 pIC50 565 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of PI3K p110beta (unknown origin) B 6.25 pIC50 562 nM IC50 J. Med. Chem. (2013) 56: 1922-1939 [PMID:23410005]
ChEMBL Inhibition of PI3Kbeta (unknown origin) B 6.42 pIC50 380 nM IC50 J Med Chem (2017) 60: 5193-5208 [PMID:28541707]
ChEMBL Inhibition of PI3Kbeta (unknown origin) after 60 mins using fluorescein-labeled kinase tracer by HTRF assay B 6.42 pIC50 380 nM IC50 Bioorg Med Chem Lett (2017) 27: 2849-2853 [PMID:28209465]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using PIP2 as substrate after 2 hrs by ADP-Glo assay B 6.55 pIC50 281 nM IC50 MedChemComm (2019) 10: 413-420
ChEMBL Inhibition of His6-tagged recombinant full length human N-terminal PI3Kbeta expressed in baculovirus infected Sf21 cells using lipid substrate incubated for 2 hrs by ADP-Glo assay B 6.81 pIC50 154 nM IC50 Eur J Med Chem (2019) 170: 112-125 [PMID:30878826]
ChEMBL Inhibition of PI3K beta (unknown origin) using phosphatidyl inositol as substrate measured after 60 mins in presence of ATP by Kinase-Glo Plus reagent-based luminescence assay B 6.86 pIC50 139.1 nM IC50 Bioorg Med Chem (2019) 27: 115035-115035 [PMID:31434616]
ChEMBL Inhibition of PI3K beta (unknown origin) using lipid substrate measured after 40 mins in presence of ATP by Kinase-Glo plus reagent based luminescence assay B 6.87 pIC50 134 nM IC50 Bioorg Med Chem (2019) 27: 114930-114930 [PMID:31176568]
ChEMBL Inhibition of PI3Kbeta (unknown origin) using Biotin-S11S12 as substrate after 60 mins in presence of ATP by ADPGlo luminescence assay B 6.89 pIC50 129 nM IC50 Eur J Med Chem (2018) 151: 9-17 [PMID:29601991]
ChEMBL Inhibition of PI3K p110beta (unknown origin) using lipid substrate after 40 mins by kinase-Glo assay B 6.92 pIC50 120.1 nM IC50 Bioorg Med Chem (2018) 26: 2028-2040 [PMID:29534936]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta/phosphoinositide-3-kinase regulatory subunit 1/PI3-kinase p110-delta/p85-alpha in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111432] [GtoPdb: 21552503] [UniProtKB: O00329P27986]
ChEMBL Inhibition of his-tagged human recombinant PIK3CD/PIK3R1 by Select-screen kinase inhibitor assay B 7.6 pIC50 25 nM IC50 J Med Chem (2017) 60: 47-65 [PMID:27644332]
ChEMBL Inhibition of His-tagged recombinant human PI3K p110delta/p85alpha expressed in Baculovirus expression system B 7.72 pIC50 19 nM IC50 J Med Chem (2020) 63: 441-469 [PMID:31550151]
ChEMBL Inhibition of human full length PI3K p110delta/p85 alpha using PIP2/ATP as substrate after 30 mins by TR-FRET assay B 7.74 pIC50 18 nM IC50 J Med Chem (2017) 60: 1555-1567 [PMID:28106991]
ChEMBL Inhibition of PI3KCdelta/PIK3R1 (unknown origin) B 7.77 pIC50 17 nM IC50 Eur J Med Chem (2018) 158: 405-413 [PMID:30237123]
ChEMBL Inhibition of PI3K p110delta/p85alpha (unknown origin) using lipid substrate after 40 mins by kinase-glo luminescence assay B 8.57 pIC50 2.7 nM IC50 Bioorg Med Chem Lett (2017) 27: 1972-1977 [PMID:28325601]
GtoPdb - - 8.6 pIC50 2.5 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of N-terminal His6-tagged recombinant full length human p110delta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence polarization assay B 8.7 pIC50 2 nM IC50 J Med Chem (2019) 62: 4936-4948 [PMID:31033293]
ChEMBL Inhibition of His-tagged recombinant human PI3K p110delta/p85alpha using lipid substrate incubated for 2 hrs by ADP-Glo assay B 8.74 pIC50 1.8 nM IC50 Eur J Med Chem (2019) 170: 112-125 [PMID:30878826]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta/PI3-kinase p110-delta subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3130] [GtoPdb: 2155] [UniProtKB: O00329]
ChEMBL Inhibition of human PI3Kalpha using PIP2 as substrate after 1 hr B 6.08 pIC50 831 nM IC50 Bioorg Med Chem Lett (2019) 29: 1313-1319 [PMID:30975623]
ChEMBL Inhibition of human PI3KCdelta by non-radiometric ADP-Glo assay B 7.6 pIC50 25 nM IC50 ACS Med. Chem. Lett. (2015) 6: 3-6 [PMID:25589915]
ChEMBL Inhibition of PI3Kdelta (unknown origin) by biochemical Alphascreen assay B 7.62 pIC50 24 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1104-1109 [PMID:25666823]
ChEMBL Inhibition of PIK3CD (unknown origin) B 7.72 pIC50 19 nM IC50 J Med Chem (2019) 62: 10005-10025 [PMID:31188592]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 8 pIC50 10 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 8 pIC50 10 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Biochemical Assays: Compounds of the invention were tested for inhibitory activity and potency against PI3Kδ. B 8.05 pIC50 9 nM IC50 US-8586597-B2. 6-fluoro-3-phenyl-2-[1-(9H-purin-6-ylamino)ethyl]-3H-quinazolin-4-one as an inhibitor of human phosphatidylinositol 3-kinase delta (2013)
ChEMBL Inhibition of PI3Kdelta B 8.05 pIC50 9 nM IC50 J. Med. Chem. (2012) 55: 8559-8581 [PMID:22924688]
ChEMBL Biochemical Assay: Using the method described in US9149477, Example 2, compounds of the invention were tested for inhibitory activity and potency against PI3Kδ, and for selectivity for PI3Kδ versus other Class I PI3K isozymes. The initial selectivity assays were performed identically to the selectivity assay protocol in US9149477, Example 2, except using 100 μL Ecoscint for radiolabel detection. Subsequent selectivity assays were done similarly using the same 3× substrate stocks except they contained 0.05 mCi/mL γ[32P]ATP and 3 mM PIP2. Subsequent selectivity assays also used the same 3× enzyme stocks, except they now contained 3 nM of any given PI3K isoform. B 8.05 pIC50 9 nM IC50 US-9149477-B2. 5-fluoro-3-phenyl-2-[1-(9h-purin-6-ylamino)propyl]-3h-quinazolin-4-one as an inhibitor of human phosphatidylinositol 3-kinase delta (2015)
ChEMBL Biochemical Assay: Using the method described in Example 2, compounds of the invention were tested for inhibitory activity and potency against PI3Kdelta, and for selectivity for PI3Kdelta versus other Class I PI3K isozymes. In Table 1, IC50 values (uM) are given for PI3Kdelta (Delta), and may be calculated for the other isoforms using the ratios of IC50 values discussed below. To illustrate selectivity of the compounds, the ratios of the IC50 values of the compounds for PI3Kalpha, PI3Kbeta, and PI3Kgamma relative to PI3Kdelta are given, respectively, as Alpha/Delta Ratio, Beta/Delta Ratio, and Gamma/Delta Ratio.The initial selectivity assays were performed identically to the selectivity assay protocol in Example 2, except, using 100 uL Ecosint for radiolabel detection. Subsequent selectivity assays were done similarly using the same 3 substrate stocks except they contained 0.05 mCi/mL gamma [32P] ATP and 3 mM PIP2. Subsequent selectivity assays also used the same 3 enzyme stocks. B 8.05 pIC50 9 nM IC50 US-8980901-B2. 5-fluoro-3-phenyl-2[1-(9H-purin-6-ylamino)propyl]-3H-quinazolin-4-one and 6-fluoro-3-phenyl-2-[1-(9H-purin-6-ylamino)ethyl]-3H-quinazolin-4-one as inhibitors of human phosphatidylinositol 3-kinase delta (2015)
ChEMBL Inhibition of PI3Kdelta in B-cells by proliferation assay B 8.21 pIC50 6.1 nM IC50 J. Med. Chem. (2012) 55: 8559-8581 [PMID:22924688]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using PIP2:PS as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 8.23 pIC50 5.9 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using PIP2 as substrate measured after 30 mins by HTRF assay B 8.37 pIC50 4.3 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of PI3Kdelta in human Ramos cells assessed as reduction in antihuman IgM-stimulated AKT phosphorylation at Ser473 residue preincubated for 60 mins followed by antihuman IgM stimulation and measured after 15 mins B 8.4 pIC50 4 nM IC50 J Med Chem (2019) 62: 4936-4948 [PMID:31033293]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using PIP2 as substrate after 2 hrs by ADP-Glo assay B 8.49 pIC50 3.2 nM IC50 MedChemComm (2019) 10: 413-420
ChEMBL Inhibition of PI3K delta (unknown origin) using lipid substrate measured after 40 mins in presence of ATP by Kinase-Glo plus reagent based luminescence assay B 8.51 pIC50 3.1 nM IC50 Bioorg Med Chem (2019) 27: 114930-114930 [PMID:31176568]
ChEMBL Inhibition of human PI3Kdelta by fluorescence-based immunoassay B 8.52 pIC50 3 nM IC50 MedChemComm (2015) 6: 2029-2035
ChEMBL Inhibition of PI3K p110delta (unknown origin) using lipid substrate after 40 mins by kinase-Glo assay B 8.57 pIC50 2.7 nM IC50 Bioorg Med Chem (2018) 26: 2028-2040 [PMID:29534936]
ChEMBL Inhibition of PI3Kdelta (unknown origin) assessed as inhibition of AKT phosphorylation by cell-based HTRF assay B 8.57 pIC50 2.7 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1104-1109 [PMID:25666823]
GtoPdb - - 8.6 pIC50 2.5 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of PI3K p110delta (unknown origin) B 8.6 pIC50 2.5 nM IC50 J. Med. Chem. (2013) 56: 1922-1939 [PMID:23410005]
ChEMBL Inhibition of human full length His-tagged PI3Kdelta expressed in baculovirus expression system B 8.6 pIC50 2.5 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of human recombinant PI3K-delta B 8.6 pIC50 2.5 nM IC50 Bioorg Med Chem Lett (2018) 28: 1005-1010 [PMID:29486969]
ChEMBL Inhibition of PI3Kdelta (unknown origin) B 8.6 pIC50 2.5 nM IC50 Bioorg Med Chem (2018) 26: 2028-2040 [PMID:29534936]
ChEMBL Inhibition of PI3Kdelta in basophil derived from B-cell malignant patient B 8.6 pIC50 2.5 nM IC50 J Med Chem (2019) 62: 4783-4814 [PMID:30582813]
ChEMBL Inhibition of PI3Kdelta (unknown origin) B 8.6 pIC50 2.5 nM IC50 MedChemComm (2012) 3: 1337-1355
ChEMBL Inhibition of human PI3Kdelta using PIP2 as substrate after 1 hr B 8.68 pIC50 2.1 nM IC50 Bioorg Med Chem Lett (2019) 29: 1313-1319 [PMID:30975623]
ChEMBL Inhibition of PI3Kdelta (unknown origin) after 60 mins using fluorescein-labeled kinase tracer by HTRF assay B 8.7 pIC50 2 nM IC50 Bioorg Med Chem Lett (2017) 27: 2849-2853 [PMID:28209465]
ChEMBL Inhibition of PI3Kdelta (unknown origin) B 8.7 pIC50 2 nM IC50 J Med Chem (2017) 60: 5193-5208 [PMID:28541707]
ChEMBL Inhibition of PI3Kdelta (unknown origin) B 8.7 pIC50 2 nM IC50 Eur J Med Chem (2019) 163: 413-427 [PMID:30530193]
ChEMBL Inhibition of human PI3Kdelta (R108 to Q1044 residues) expressed in mammalian expression system incubated for 60 mins by ADAPTA assay B 8.7 pIC50 2 nM IC50 ACS Med Chem Lett (2016) 7: 908-912 [PMID:27774127]
ChEMBL Inhibition of PI3Kdelta (unknown origin) using Biotin-S11S12 as substrate after 120 mins in presence of ATP by ADPGlo luminescence assay B 8.8 pIC50 1.6 nM IC50 Eur J Med Chem (2018) 151: 9-17 [PMID:29601991]
ChEMBL Inhibition of PI3K delta (unknown origin) using phosphatidyl inositol as substrate measured after 60 mins in presence of ATP by Kinase-Glo Plus reagent-based luminescence assay B 8.92 pIC50 1.2 nM IC50 Bioorg Med Chem (2019) 27: 115035-115035 [PMID:31434616]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma/PI3-kinase p110-gamma subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3267] [GtoPdb: 2156] [UniProtKB: P48736]
ChEMBL Inhibition of His-tagged recombinant human PIK3CG expressed in Baculovirus expression system B 5.68 pIC50 2100 nM IC50 J Med Chem (2020) 63: 441-469 [PMID:31550151]
ChEMBL Inhibition of PI3Kgamma (unknown origin) by biochemical Alphascreen assay B 6.24 pIC50 580 nM IC50 Bioorg. Med. Chem. Lett. (2015) 25: 1104-1109 [PMID:25666823]
ChEMBL Inhibition of PI3K gamma (unknown origin) using lipid substrate measured after 40 mins in presence of ATP by Kinase-Glo plus reagent based luminescence assay B 6.42 pIC50 381 nM IC50 Bioorg Med Chem (2019) 27: 114930-114930 [PMID:31176568]
ChEMBL Inhibition of PI3KCgamma (unknown origin) B 6.6 pIC50 250 nM IC50 Eur J Med Chem (2018) 158: 405-413 [PMID:30237123]
ChEMBL Inhibition of PI3Kgamma (unknown origin) using PIP2 as substrate measured after 30 mins by HTRF assay B 6.62 pIC50 241 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of PI3Kgamma (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 6.7 pIC50 199.53 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3Kgamma (unknown origin) using biotin-PIP3 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence polarization assay B 6.7 pIC50 199.53 nM IC50 J Med Chem (2019) 62: 10402-10422 [PMID:31647659]
ChEMBL Inhibition of PI3K gamma (unknown origin) using phosphatidyl inositol as substrate measured after 60 mins in presence of ATP by Kinase-Glo Plus reagent-based luminescence assay B 6.84 pIC50 145.3 nM IC50 Bioorg Med Chem (2019) 27: 115035-115035 [PMID:31434616]
ChEMBL Inhibition of PI3K p110gamma (unknown origin) using lipid substrate after 40 mins by kinase-Glo assay B 6.86 pIC50 139.4 nM IC50 Bioorg Med Chem (2018) 26: 2028-2040 [PMID:29534936]
ChEMBL Inhibition of PI3Kgamma (unknown origin) using Biotin-S11S12 as substrate after 60 mins in presence of ATP by ADPGlo luminescence assay B 6.86 pIC50 139 nM IC50 Eur J Med Chem (2018) 151: 9-17 [PMID:29601991]
ChEMBL Inhibition of recombinant human full-length His-tagged PI3K p110gamma expressed in baculovirus expression system using PIP2 as substrate measured after 80 mins by transcreener fluorescence polarization assay B 6.98 pIC50 104 nM IC50 J Med Chem (2019) 62: 4936-4948 [PMID:31033293]
ChEMBL Inhibition of human PI3K 110gamma by fluorescence-based immunoassay B 7.04 pIC50 92 nM IC50 MedChemComm (2015) 6: 2029-2035
ChEMBL Inhibition of human PI3Kgamma using PIP2 as substrate after 1 hr B 7.04 pIC50 92 nM IC50 Bioorg Med Chem Lett (2019) 29: 1313-1319 [PMID:30975623]
GtoPdb - - 7.05 pIC50 89 nM IC50 Blood (2011) 117: 591-4 [PMID:20959606]
ChEMBL Inhibition of PI3K p110gamma (unknown origin) B 7.05 pIC50 89 nM IC50 J. Med. Chem. (2013) 56: 1922-1939 [PMID:23410005]
ChEMBL Inhibition of recombinant human full length His-tagged PI3Kgamma expressed in insect cells B 7.05 pIC50 89 nM IC50 Eur J Med Chem (2017) 125: 1156-1171 [PMID:27846451]
ChEMBL Inhibition of PI3Kgamma in basophil derived from B-cell malignant patient B 7.05 pIC50 89 nM IC50 J Med Chem (2019) 62: 4783-4814 [PMID:30582813]
ChEMBL Inhibition of PI3Kgamma (unknown origin) B 7.05 pIC50 89 nM IC50 MedChemComm (2012) 3: 1337-1355
ChEMBL Inhibition of PI3Kgamma (unknown origin) using PIP2:PS as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr in presence of 50 uM ATP by ADP-Glo luminescence assay B 7.17 pIC50 67 nM IC50 Eur J Med Chem (2018) 156: 831-846 [PMID:30053721]
ChEMBL Inhibition of human PI3Kgamma (S144 to A1102 residues) expressed in mammalian expression system incubated for 60 mins by ADAPTA assay B 7.19 pIC50 64 nM IC50 ACS Med Chem Lett (2016) 7: 908-912 [PMID:27774127]
ChEMBL Inhibition of PI3Kgamma (unknown origin) after 60 mins using fluorescein-labeled kinase tracer by HTRF assay B 7.2 pIC50 63 nM IC50 Bioorg Med Chem Lett (2017) 27: 2849-2853 [PMID:28209465]
ChEMBL Inhibition of PI3Kgamma (unknown origin) B 7.2 pIC50 63 nM IC50 Eur J Med Chem (2019) 163: 413-427 [PMID:30530193]
ChEMBL Inhibition of His-tagged recombinant human full length PI3K p110gamma expressed in baculovirus expression system using lipid substrate incubated for 2 hrs by ADP-Glo assay B 7.2 pIC50 63 nM IC50 Eur J Med Chem (2019) 170: 112-125 [PMID:30878826]
ChEMBL Inhibition of PI3Kgamma (unknown origin) B 7.2 pIC50 63 nM IC50 J Med Chem (2017) 60: 5193-5208 [PMID:28541707]
ChEMBL Inhibition of PI3Kgamma (unknown origin) using PIP2 as substrate after 2 hrs by ADP-Glo assay B 7.22 pIC50 60 nM IC50 MedChemComm (2019) 10: 413-420
phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3K p110 beta/p85 alpha in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038510] [GtoPdb: 25032154] [UniProtKB: P27986P42338]
ChEMBL Inhibition of human full length recombinant N-terminal His-tagged PI3Kbeta/p85alpha expressed in baculovirus infected Sf21 insect cells B 5.42 pIC50 3807 nM IC50 Eur J Med Chem (2019) 163: 413-427 [PMID:30530193]
ChEMBL Inhibition of N-terminal His6-tagged recombinant full-length human PI3K p110beta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2/ATP as substrate after 30 mins by TR-FRET assay B 5.43 pIC50 3700 nM IC50 J Med Chem (2017) 60: 1555-1567 [PMID:28106991]